CX295728

Overview
NameCX295728
Unique NameCX295728
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length720
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_STELP (Triosephosphate isomerase, cytosolic OS=Stellaria longipes GN=TPI PE=2 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 8.330e-70
Identity = 137/206 (66.50%), Postives = 153/206 (74.27%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIAD-RVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLK-PEFIDIIKSAELK 671
            FL    + L+ +  VAAQNCWVKKGGAFTGE+SA+ML NL I WVILGHSERR +L ESNEFVG K AYA S+GL VIAC+GE LE+REAG T DV   Q K+ A      W N+V+AYEPVWAIGTGKVA+P QAQEVH  +R WL  N S E+A+ TRIIYGGSVNG N   LAAQ DVDGFLVGGASLK PEF  II S   K
Sbjct:   48 FLPDENAELKHEIQVAAQNCWVKKGGAFTGEVSAQMLANLGITWVILGHSERRTLLGESNEFVGKKAAYAQSEGLGVIACIGELLEEREAGKTFDVCFKQLKSFARFPAKPWDNVVVAYEPVWAIGTGKVASPEQAQEVHVAVRDWLKTNVSEEVASKTRIIYGGSVNGGNSLALAAQEDVDGFLVGGASLKGPEFATIINSVTAK 253          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIC_SECCE (Triosephosphate isomerase, chloroplastic OS=Secale cereale PE=1 SV=1)

HSP 1 Score: 261.151 bits (666), Expect = 4.134e-69
Identity = 128/205 (62.44%), Postives = 154/205 (75.12%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLK-PEFIDIIKSAELK 671
            ++  VKSSL     V+AQN W+ KGGAFTGEISAE LV++   WVILGHSERR ++ E +EF+G K AYALSQ LKV+AC+GE LE+REAG T DV   Q KA AD ++ W+N+V+AYEPVWAIGTGKVA+P QAQEVH  +R WL  N S ++A+  RIIYGGSVN ANC ELA + D+DGFLVGGASLK P+F  I  S   K
Sbjct:   90 YIDQVKSSLTDRIEVSAQNTWIGKGGAFTGEISAEQLVDIGCQWVILGHSERRHVIGEDDEFIGKKAAYALSQNLKVMACIGELLEEREAGKTFDVCFKQMKAFADNITDWTNVVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKTNVSADVASTVRIIYGGSVNAANCAELAKKEDIDGFLVGGASLKGPDFATICNSVTSK 294          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_RABIT (Triosephosphate isomerase OS=Oryctolagus cuniculus GN=TPI1 PE=1 SV=1)

HSP 1 Score: 260.766 bits (665), Expect = 5.399e-69
Identity = 128/202 (63.37%), Postives = 152/202 (75.25%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR +  ES+E +G KVA+ALS+GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   47 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALSEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 248          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIC_ARATH (Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana GN=TIM PE=1 SV=1)

HSP 1 Score: 260.381 bits (664), Expect = 7.052e-69
Identity = 132/205 (64.39%), Postives = 152/205 (74.15%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLK-PEFIDIIKSAELK 671
            ++  VKSSL     ++ QN WV KGGAFTGEIS E L +L   WVILGHSERR ++ E +EF+G K AYALS+GL VIAC+GE LE+REAG T DV  AQ KA AD V SW NIV+AYEPVWAIGTGKVA+P QAQEVH  +R WL  N S E+A+ TRIIYGGSVNG N  ELA + D+DGFLVGGASLK PEF  I+ S   K
Sbjct:  107 YIDQVKSSLTDRIDISGQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVIACIGEKLEEREAGKTFDVCFAQLKAFADAVPSWDNIVVAYEPVWAIGTGKVASPQQAQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFATIVNSVTSK 311          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_PONAB (Triosephosphate isomerase OS=Pongo abelii GN=TPI1 PE=2 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 1.203e-68
Identity = 127/202 (62.87%), Postives = 152/202 (75.25%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDYGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_PANTR (Triosephosphate isomerase OS=Pan troglodytes GN=TPI1 PE=2 SV=2)

HSP 1 Score: 259.61 bits (662), Expect = 1.203e-68
Identity = 127/202 (62.87%), Postives = 152/202 (75.25%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_MACMU (Triosephosphate isomerase OS=Macaca mulatta GN=TPI1 PE=3 SV=2)

HSP 1 Score: 259.61 bits (662), Expect = 1.203e-68
Identity = 127/202 (62.87%), Postives = 152/202 (75.25%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSEAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_MACFA (Triosephosphate isomerase OS=Macaca fascicularis GN=TPI1 PE=2 SV=3)

HSP 1 Score: 259.61 bits (662), Expect = 1.203e-68
Identity = 127/202 (62.87%), Postives = 152/202 (75.25%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSEAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_HUMAN (Triosephosphate isomerase OS=Homo sapiens GN=TPI1 PE=1 SV=2)

HSP 1 Score: 259.61 bits (662), Expect = 1.203e-68
Identity = 127/202 (62.87%), Postives = 152/202 (75.25%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_BOVIN (Triosephosphate isomerase OS=Bos taurus GN=TPI1 PE=2 SV=3)

HSP 1 Score: 259.61 bits (662), Expect = 1.203e-68
Identity = 127/202 (62.87%), Postives = 152/202 (75.25%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +L   WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A + RIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVANGAFTGEISPGMIKDLGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSARIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
The following BLAST results are available for this feature:
BLAST of CX295728 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIS_STELP8.330e-7066.50Triosephosphate isomerase, cytosolic OS=Stellaria ... [more]
TPIC_SECCE4.134e-6962.44Triosephosphate isomerase, chloroplastic OS=Secale... [more]
TPIS_RABIT5.399e-6963.37Triosephosphate isomerase OS=Oryctolagus cuniculus... [more]
TPIC_ARATH7.052e-6964.39Triosephosphate isomerase, chloroplastic OS=Arabid... [more]
TPIS_PONAB1.203e-6862.87Triosephosphate isomerase OS=Pongo abelii GN=TPI1 ... [more]
TPIS_PANTR1.203e-6862.87Triosephosphate isomerase OS=Pan troglodytes GN=TP... [more]
TPIS_MACMU1.203e-6862.87Triosephosphate isomerase OS=Macaca mulatta GN=TPI... [more]
TPIS_MACFA1.203e-6862.87Triosephosphate isomerase OS=Macaca fascicularis G... [more]
TPIS_HUMAN1.203e-6862.87Triosephosphate isomerase OS=Homo sapiens GN=TPI1 ... [more]
TPIS_BOVIN1.203e-6862.87Triosephosphate isomerase OS=Bos taurus GN=TPI1 PE... [more]

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Properties
Property NameValue
Genbank descriptionC05075C06SK DroRLeaf1 Citrus clementina cDNA clone C05075C06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295728 ID=CX295728; Name=CX295728; organism=Citrus clementina; type=EST; length=720bp
CTGAAGGTCAAGTTCCATCTTCTGATGTCGTTGAGGTCGTTGTTAGCCCT
CCGTTTGTGTTTCTTGGACTGGTGAAGAGTTCGTTGAGGCCTGATTTTCA
TGTTGCGGCACAGAATTGCTGGGTCAAGAAGGGAGGTGCTTTCACCGGTG
AGATTAGTGCGGAAATGCTCGTCAATTTGGATATTCCTTGGGTCATTCTT
GGTCATTCTGAAAGGAGACTCATTTTGAATGAATCAAATGAGTTTGTTGG
GGATAAGGTTGCATATGCACTTTCTCAAGGTTTGAAGGTGATTGCTTGTG
TTGGTGAGACACTTGAGCAGCGTGAAGCAGGATCTACTATGGATGTTGTT
GCTGCACAAACTAAAGCAATCGCAGATCGAGTCTCAAGCTGGTCAAACAT
TGTTTTGGCCTATGAACCTGTGTGGGCTATTGGAACTGGGAAGGTTGCCA
CTCCAGTGCAGGCTCAAGAAGTACATTTTGAACTGAGGAAATGGCTTTTT
GCAAACACCAGTCCTGAAATTGCTGCAGCAACACGGATCATCTACGGAGG
ATCCGTAAATGGTGCAAACTGCAAGGAATTGGCTGCACAGCCTGATGTTG
ACGGATTTTTGGTTGGTGGTGCTTCGTTGAAGCCGGAGTTCATTGACATC
ATCAAATCTGCTGAGCTGAAGTAAATTCTTGAGCTTTATTTCAGGGCAGT
TTGCTGATGAGTATTACTGT
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