CX296271

Overview
NameCX296271
Unique NameCX296271
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length710
Libraries
Library NameType
FerrChloL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig9336 contig Ccv1_Contig9336:541..1249. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 1.924e-18
Identity = 43/71 (60.56%), Postives = 56/71 (78.87%), Query Frame = -3 
Query:  496 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 708
            TLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    7 TLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 78.9518 bits (193), Expect = 2.875e-14
Identity = 36/60 (60.00%), Postives = 48/60 (80.00%), Query Frame = -3 
Query:  313 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQ 492
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIE 62
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.282e-18
Identity = 42/70 (60.00%), Postives = 54/70 (77.14%), Query Frame = -3 
Query:  499 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 708
            TLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    7 TLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 80.8777 bits (198), Expect = 7.567e-15
Identity = 36/61 (59.02%), Postives = 48/61 (78.69%), Query Frame = -3 
Query:  316 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 498
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKV 61
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.629e-17
Identity = 40/70 (57.14%), Postives = 54/70 (77.14%), Query Frame = -3 
Query:  499 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 708
            TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    7 TLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 75.8702 bits (185), Expect = 2.434e-13
Identity = 32/65 (49.23%), Postives = 49/65 (75.38%), Query Frame = -3 
Query:  304 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKES 498
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGS 65
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.740e-17
Identity = 39/70 (55.71%), Postives = 54/70 (77.14%), Query Frame = -3 
Query:  499 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 708
            TLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    7 TLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.093e-13
Identity = 33/61 (54.10%), Postives = 48/61 (78.69%), Query Frame = -3 
Query:  316 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 498
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKI 61
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.740e-17
Identity = 39/70 (55.71%), Postives = 54/70 (77.14%), Query Frame = -3 
Query:  499 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 708
            TLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    7 TLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.093e-13
Identity = 33/61 (54.10%), Postives = 48/61 (78.69%), Query Frame = -3 
Query:  316 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 498
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKI 61
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 4.740e-17
Identity = 39/70 (55.71%), Postives = 54/70 (77.14%), Query Frame = -3 
Query:  499 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 708
            TLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    7 TLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.093e-13
Identity = 33/61 (54.10%), Postives = 48/61 (78.69%), Query Frame = -3 
Query:  316 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 498
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKI 61
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.740e-17
Identity = 39/70 (55.71%), Postives = 54/70 (77.14%), Query Frame = -3 
Query:  499 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 708
            TLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    7 TLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.093e-13
Identity = 33/61 (54.10%), Postives = 48/61 (78.69%), Query Frame = -3 
Query:  316 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 498
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKI 61
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.740e-17
Identity = 39/70 (55.71%), Postives = 54/70 (77.14%), Query Frame = -3 
Query:  499 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 708
            TLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    7 TLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.093e-13
Identity = 33/61 (54.10%), Postives = 48/61 (78.69%), Query Frame = -3 
Query:  316 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 498
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKI 61
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 5.240e-16
Identity = 37/71 (52.11%), Postives = 54/71 (76.06%), Query Frame = -3 
Query:  496 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 708
            TLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    7 TLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 72.0182 bits (175), Expect = 3.515e-12
Identity = 31/62 (50.00%), Postives = 47/62 (75.81%), Query Frame = -3 
Query:  313 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQ 498
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIE 62
[Close]
BLAST of CX296271 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.167e-15
Identity = 38/70 (54.29%), Postives = 52/70 (74.29%), Query Frame = -3 
Query:  499 TLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 708
            TLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    7 TLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 73.1738 bits (178), Expect = 1.578e-12
Identity = 33/65 (50.77%), Postives = 48/65 (73.85%), Query Frame = -3 
Query:  304 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKES 498
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGS 65
The following BLAST results are available for this feature:
BLAST of CX296271 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 95
Match NameE-valueIdentityDescription
RUB2_ARATH1.924e-1860.56NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
NEDD8_CAEEL3.282e-1860.00NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB3_ORYSJ1.629e-1757.14NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
NEDD8_RAT4.740e-1755.71NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT4.740e-1755.71NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE4.740e-1755.71NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN4.740e-1755.71NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN4.740e-1755.71NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
NEDD8_DICDI5.240e-1652.11NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]
RUB3_ARATH1.167e-1554.29NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]

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Properties
Property NameValue
Genbank descriptionC05138B10SK FerrChloL1 Citrus clementina cDNA clone C05138B10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX296271 ID=CX296271; Name=CX296271; organism=Citrus clementina; type=EST; length=710bp
GGAATCTCATCTTTTTCATTGCAAAGTAGAACTGCATTGCAAACCGAAAA
ATCAATTATCACACAAATCAAAACCAACATTATAGTAATATATTTAAACT
AGAGTTTTTTATTCAAAAGCATAACTCGTTCAACAAACAACAACATATAA
AAACAAAAGACCATATTATCAATTATAACTAGTCTTCTTCAAGAAGACAA
AGACAACATAAGGACCATCTTGAACAGAAAAGACATTGACACGGGGCGCA
GTGAATCCCCCTCAGAGACCACCACCTCGGAGACGGAGGACGAGATGGAG
GGTGGACTCCTTCTGAATGTTGTAATCAGCCAGAGTACGCCCGTCCTCAA
GCTGCTTCCCAGCGAAGATGAGCCTCTGCTGATCAGGTGGGATCCCTTCC
TTGTCCTGAATCTTGGCTTTGACATTGTCAATGGTATCAGAACTCTCTAC
CTCCAGGGTAATCGTCTTCCCGGTCAATGTCTTAACAAAAATCTGCATGC
CACCCCTCAGACGCAAAACCAGATGAAGAGTTGATTCCTTCTGAATGTTG
TAATCCGCGAGCGTCCTTCCATCCTCCAGCTGCTTGCCAGCGAAGATCAA
ACGCTGCTGGTCCGGCGGAATCCCTTCCTTGTCCTGGATCTTGGCCTTGA
CGTTGTCGATCGTGTCGGAGCTCTCGACTTCGAGGGTAATCGTCTTTCCC
GTTAGGGTTT
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