DY258284

Overview
NameDY258284
Unique NameDY258284
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1315
Libraries
Library NameType
AbsDev1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: PLK4_DROSE (Serine/threonine-protein kinase PLK4 OS=Drosophila sechellia GN=SAK PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 65/224 (29.02%), Postives = 105/224 (46.88%), Query Frame = 3
Query:  180 DGFSNKNILGRGGFGKVYKGR-LADGKLVAVKRLKEERTSGGEL--QFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKD-THITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELITGQRAFDLAMLANDENVMLL 839
            + +  +++LG+GGF  VYK R L   + VA+K + ++   G  L  + + EV+I S   H ++L L  F        LV     NG +   +          +  +  K  +    GL YLH H    I+HRD+  +N+LL  E    + DFGLA  +   D  H+T    GT  +I+PE +S        DV+  G ML  L+ G+  F+   + +  N +++
Sbjct:   12 EDYEVQHLLGKGGFATVYKARCLHTHQDVAIKMIDKKLIQGTGLTSRVRQEVEIHSRLKHPSVLQLYTFFQDANYVYLVLELAHNGELHRYMNHIARHFTETEAASILKQVVA---GLLYLHSH---NIMHRDISLSNLLLSREMHVKIADFGLATQLKRPDERHMTMC--GTPNYISPEVVSRSSHGLPADVWSVGCMLYTLLVGRPPFETDAVQSTLNKVVM 227          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: LIMK2_CHICK (LIM domain kinase 2 OS=Gallus gallus GN=LIMK2 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 66/215 (30.70%), Postives = 98/215 (45.58%), Query Frame = 3
Query:  201 ILGRGGFGKVYK-GRLADGKLVAVKRLK--EERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKL-------------------MDYKDTHITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELI 779
            +LG+G FG+  K    A GK++ +K L   +E T   +  F TEVK++    H N+L   G      +  L+  Y+  G++   LR       P  W  +   A G A G++YLH  C   IIHRD+ + N L+  +   VV DFGL++L                   +   D      V G    +APE L+     E  D+F +GI+L E+I
Sbjct:  336 VLGKGFFGQAIKVTHKATGKVMVMKELIRCDEET---QKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNAD----PFPWQQKVSFAKGIASGMAYLHSMC---IIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKKPTLEKPSAKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGQSYDETVDIFSFGIVLCEII 540          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: LIMK2_BOVIN (LIM domain kinase 2 OS=Bos taurus GN=LIMK2 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 66/215 (30.70%), Postives = 100/215 (46.51%), Query Frame = 3
Query:  201 ILGRGGFGKVYK-GRLADGKLVAVKRLK--EERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKL-------------------MDYKDTHITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELI 779
            +LG+G FG+  K    A GK++ +  L   +E T   +  F TEVK++    H N+L   G      +  L+  Y+  G++   LR    ++ P  W  + + A G A G++YLH  C   IIHRD+ + N L+  +   VV DFGL++L                   +   D      V G    +APE L+     E  DVF +GI+L E+I
Sbjct:  336 VLGKGFFGQAIKVTHKATGKVMVMNELIRCDEET---QKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLR----NVDPFPWQQKVRFAKGIASGMAYLHSMC---IIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKKPPVEKATTKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEII 540          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: INSR_AEDAE (Insulin-like receptor OS=Aedes aegypti GN=InR PE=2 SV=2)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 62/209 (29.67%), Postives = 100/209 (47.85%), Query Frame = 3
Query:  204 LGRGGFGKVYKGRLAD------GKLVAVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSL-----PPLDWPTRKKI---ALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHG--TVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT 782
            LG+G FG VYKG L         +  A+K + E  T+  +  F  E  ++      +++ L G  +     L++   MANG + S LR  +        P    PT ++I   A+  A G++YL      K +HRD+ A N ++ ++    +GDFG+ + + Y+  +      G   V  +APE L  G  S  +DVF YG++L E+ T
Sbjct: 1031 LGQGSFGMVYKGILTQLRGEKCNQPCAIKTVNESATAREKDSFLLEASVMKQFNTHHVVRLLGVVSQGDPTLVIMELMANGDLKSYLRRHRPDYENGEDPSPQPPTLRQIIQMAIEIADGMAYLSAK---KFVHRDLAARNCMVADDMTVKIGDFGMTRDI-YETDYYRKGTKGFLPVRWMAPESLKDGIFSSSSDVFSYGVVLWEMAT 1235          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: FPS_DROME (Tyrosine-protein kinase Fps85D OS=Drosophila melanogaster GN=Fps85D PE=1 SV=3)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 59/197 (29.95%), Postives = 101/197 (51.27%), Query Frame = 3
Query:  204 LGRGGFGKVYKGRLADGKL-VAVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIAL--GSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHG-TVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT 782
            +GRG FG VYK +L   KL VAVK  +       + +F  E +I+    H N++ L G C      ++V   +  GS+ + LR+  + L      TR+++ +   +A G+ YL        IHRD+ A N L+D E    + DFG+++  + ++  ++  +    V   APE L+ G+ +   DV+ YGI++ E+ +
Sbjct: 1069 IGRGNFGDVYKAKLKSTKLDVAVKTCRMTLPDEQKRKFLQEGRILKQYDHPNIVKLIGICVQKQPIMIVMELVLGGSLLTYLRKNSNGL-----TTRQQMGMCRDAAAGMRYLE---SKNCIHRDLAARNCLVDLEHSVKISDFGMSR--EEEEYIVSDGMKQIPVKWTAPEALNFGKYTSLCDVWSYGILMWEIFS 1255          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: EPHA5_RAT (Ephrin type-A receptor 5 OS=Rattus norvegicus GN=Epha5 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 62/208 (29.81%), Postives = 103/208 (49.52%), Query Frame = 3
Query:  195 KNILGRGGFGKVYKGRLA-DGKL---VAVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLM--DYKDTHITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT-GQRAF 797
            + ++G G FG+V  GRL   GK    VA K LK   T      F +E  I+    H N++ L G  T     ++V  YM NGS+ + L++       +      +   G A G+ YL    D   +HRD+ A NIL++      V DFGL++++  D +  + T      +   APE ++  + +  +DV+ YGI++ E+++ G+R +
Sbjct:  680 ERVIGAGEFGEVCSGRLKLPGKRELPVATKTLKVGYTEKQRRDFLSEASIMGQFDHPNIIHLEGVVTKSKPVMIVTEYMENGSLDTFLKKNDGQFTVIQLVGMLR---GIAAGMKYL---SDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAFRKFTSASDVWSYGIVMWEVVSYGERPY 881          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: EGFR_DROME (Epidermal growth factor receptor OS=Drosophila melanogaster GN=Egfr PE=1 SV=3)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 68/225 (30.22%), Postives = 117/225 (52.00%), Query Frame = 3
Query:  153 SLRELQVATDGFSNKN-ILGRGGFGKVYKGR-LADGKLV----AVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPP---LDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHG--TVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT-GQR 791
            +L +L++  D    K  +LG G FG+VYKG  + +G+ V    A+K L +   +    +F  E  I++   H NLL L   C + ++ +L+ + M  G +   +R  +  +     L+W T+       A+G+SYL E    +++HRD+ A N+L+       + DFGLAKL+   D++   A  G   +  +A E +     + K+DV+ +G+ + EL+T GQR
Sbjct:  926 NLCKLRIVKDAELRKGGVLGMGAFGRVYKGVWVPEGENVKIPVAIKELLKSTGAESSEEFLREAYIMASEEHVNLLKLLAVCMS-SQMMLITQLMPLGCLLDYVRNNRDKIGSKALLNWSTQ------IAKGMSYLEEK---RLVHRDLAARNVLVQTPSLVKITDFGLAKLLS-SDSNEYKAAGGKMPIKWLALECIRNRVFTSKSDVWAFGVTIWELLTFGQR 1139          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: CIPKP_ARATH (CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana GN=CIPK25 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 69/249 (27.71%), Postives = 118/249 (47.39%), Query Frame = 3
Query:  117 DSELQLGQLKRFSLRELQVATDGFSNKNILGRGGFGKVYKGR-LADGKLVAVKRLKEERTS--GGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMD--YKDTHITTAVHGTVGHIAPEYL-STGRSSEKTDVFGYGIMLLELITGQRAFDLAMLANDENVMLLDR 845
            DS  Q         ++L+V    +    +LG+G FGKVY G+ +  G+ VA+K + +++    G   Q + E+ I+ +  H N++ L     T T+   +  Y+  G + S++ + +     L   + +K        + + H      + HRD+K  N+L+DE  +  V DFGL+ L +   +D  + T   GT  ++APE L   G    K D++  GI+L  L+ G   F       DEN+M + R
Sbjct:   20 DSRYQSAPTMEEEQQQLRVLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREISIMRLVRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKIVKGK-----LKEDSARKYFQQLISAVDFCHSR---GVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQC-GTPAYVAPEVLRKKGYDGAKGDIWSCGIILYVLLAGFLPF------QDENLMKMYR 253          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: STK_HYDAT (Tyrosine-protein kinase STK OS=Hydra attenuata GN=STK PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 8.497e-15
Identity = 61/196 (31.12%), Postives = 95/196 (48.47%), Query Frame = 3
Query:  204 LGRGGFGKVYKGRLADGKLVAVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELITGQR 791
            LG G FG+V+ G   +   VAVK LK    S     F  E  ++    H++L+ L   C+      +V  YM+ GS+   L + +     L  PT   +A   A G+++L        IHRD+ A NIL+ E +   V DFGLA+L++  +          +   APE     R + K+DV+ +GI++ E++T  R
Sbjct:  246 LGAGQFGEVWAGVWNNTTQVAVKTLKPGTMSPAS--FLDEAGVMKKLRHKHLVQLYAICSDREPIYIVTEYMSGGSLLDYLSKGEGV--NLQLPTLIDMAAQVASGMAFLEAQ---GYIHRDLAARNILVGENYICKVADFGLARLIEDDEYTAHEGAKFPIKWTAPEAALYNRFTIKSDVWSFGILMAEIVTKGR 434          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: STK6_MOUSE (Serine/threonine-protein kinase 6 OS=Mus musculus GN=Aurka PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 8.497e-15
Identity = 63/233 (27.04%), Postives = 103/233 (44.21%), Query Frame = 3
Query:  114 DDSELQLGQLKRFS-LRELQVATDGFSNKNILGRGGFGKVYKGRLADGKLVAVKRL---KEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELITGQRAFD 800
            +DSE +   L++    ++ Q   + F     LG+G FG VY  R    K +   ++    +   +  E Q + EV+I S   H N+L L G+    T   L+  Y   G+V   L++    L   D           A  LSY H     ++IHRD+K  N+LL    E  + DFG +    +  +   T + GT+ ++ PE +      EK D++  G++  E + G   F+
Sbjct:   99 NDSEKEQASLQKTEDTKKRQWTLEDFDIGRPLGKGKFGNVYLARERQSKFILALKVLFKTQLEKANVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQK----LSKFDEQRTATYITELANALSYCHSK---RVIHRDIKPENLLLGSNGELKIADFGWSV---HAPSSRRTTMCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGMPPFE 321          
The following BLAST results are available for this feature:
BLAST of DY258284 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
PLK4_DROSE6.506e-1529.02Serine/threonine-protein kinase PLK4 OS=Drosophila... [more]
LIMK2_CHICK6.506e-1530.70LIM domain kinase 2 OS=Gallus gallus GN=LIMK2 PE=2... [more]
LIMK2_BOVIN6.506e-1530.70LIM domain kinase 2 OS=Bos taurus GN=LIMK2 PE=2 SV... [more]
INSR_AEDAE6.506e-1529.67Insulin-like receptor OS=Aedes aegypti GN=InR PE=2... [more]
FPS_DROME6.506e-1529.95Tyrosine-protein kinase Fps85D OS=Drosophila melan... [more]
EPHA5_RAT6.506e-1529.81Ephrin type-A receptor 5 OS=Rattus norvegicus GN=E... [more]
EGFR_DROME6.506e-1530.22Epidermal growth factor receptor OS=Drosophila mel... [more]
CIPKP_ARATH6.506e-1527.71CBL-interacting serine/threonine-protein kinase 25... [more]
STK_HYDAT8.497e-1531.12Tyrosine-protein kinase STK OS=Hydra attenuata GN=... [more]
STK6_MOUSE8.497e-1527.04Serine/threonine-protein kinase 6 OS=Mus musculus ... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAI1AA05FM2 AbsDev1 Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY258284 ID=DY258284; Name=DY258284; organism=Citrus clementina; type=EST; length=1315bp
TTCCTGTGGGAGTTGCTCTTGGCGCTGCTTTACTTTTTGCTGTACCAGTT
ATTGGGTTTGCATATTGGCGGCGAACAAGACCACACGAATTTTTCTTTGA
CGTACCTGCTGAAGATGATTCAGAACTCCAGTTGGGACAGTTGAAAAGGT
TTTCTTTGCGAGAACTACAAGTTGCTACAGATGGTTTTAGCAACAAGAAC
ATCCTGGGAAGAGGTGGATTTGGCAAGGTGTATAAGGGACGCTTGGCTGA
TGGCAAATTGGTGGCAGTCAAAAGACTTAAGGAAGAGAGGACATCTGGAG
GGGAGTTGCAGTTTCAAACAGAAGTGAAGATAATCAGCATGGCTGTGCAT
CGGAATCTTCTGGGATTATGTGGGTTTTGCACGACTGTCACTGAGAAACT
GCTTGTTTATCGATACATGGCCAATGGAAGTGTTGCATCACGCCTAAGAG
AGCGGCAATCATCACTCCCGCCTCTTGACTGGCCAACTAGGAAGAAAATT
GCTTTAGGTTCAGCGAGGGGCCTTTCATATTTGCACGAGCATTGTGACCC
AAAGATCATTCATCGAGATGTCAAAGCTGCAAACATATTGTTGGATGAAG
AGTTTGAGGCTGTCGTTGGTGACTTTGGTTTAGCTAAACTAATGGACTAC
AAGGACACCCACATAACCACTGCTGTGCATGGCACCGTTGGGCATATAGC
CCCAGAGTATCTCTCCACAGGGAGGTCATCAGAGAAAACGGATGTTTTTG
GTTACGGTATCATGCTGTTAGAGCTAATTACCGGACAAAGGGCATTTGAT
CTTGCTATGCTTGCAAACGATGAAAATGTTATGCTCCTTGATAGAGTTAA
AGGATACCCTANGAGAGAAGAATTTTGATGTACTTGTTGATAAAGACCTG
CCGACTGATTATGAATGAAACTGAAGTGGGAAGAACTCCTCCCAAGTGGC
CCTCCCTTGCCCCCCAACCCCCCCCATCCCACGGGCCCAAAAAAGTCGAA
ATTTGCCAAAATGCTTGAAAGGGCATGGGCTGGGCAAAAAAAAAGGGGGC
AGGGGGGCCAACGGCCGGAGGGTATCCCCCCCCCAAGAAATCTTCATTTG
CCCCACCGGGCAAAAAATTTAAAAAAAAAAAAATTTACCCTCCCCCCCCA
CCCCTTTTTCAAAAATTGCTTTTTTCCCGCGGGCCGCCAAAAAAGAAAAA
GATTTTTTTCCTGTTGGGGCAAACAAAAAGAAAAAACCACCACAACCCCC
CCCTCTTCTCCCCCCTCCCCCCCCCTATTAATTAAGGGAGGGGGGGGTTT
GATTAAACCCCCCCC
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