Construction of a citrus framework genetic map anchored by 708 gene-based markers

Publication Overview
TitleConstruction of a citrus framework genetic map anchored by 708 gene-based markers
AuthorsShimada T, Fujii H, Endo T, Ueda T, Sugiyama A, Nakano M, Kita M, Yoshioka T, Shimizu T, Nesumi H, Ikoma Y, Moriguchi T, Omura M
TypeJournal Article
Journal NameTree genetics & genomes
Volume10
Issue4
Year2014
Page(s)1001-1013
CitationShimada T, Fujii H, Endo T, Ueda T, Sugiyama A, Nakano M, Kita M, Yoshioka T, Shimizu T, Nesumi H, Ikoma Y, Moriguchi T, Omura M. Construction of a citrus framework genetic map anchored by 708 gene-based markers. Tree genetics & genomes. 2014; 10(4):1001-1013.

Abstract

We developed 708 gene-based markers for citrus genome analysis. Sequence-tagged site (STS) primers were designed that were located in conserved exon regions and whose PCR products spanned genomic introns. Of these, 79.7 % comprised cleaved amplified polymorphic sequence markers. The gene-based markers and their annotation and position on Clementine scaffolds ver. 1.0 permitted comparison of the genetic map and the Clementine genome sequence. The 708 gene-based markers were used to construct a genetic map using the 87 progenies (AG population) from the cross between ‘Okitsu 46 gou’ (‘Sweet Spring’ (‘Ueda unshiu’ (Citrus unshiu) × Hassaku (Citrus hassaku Hort. ex Tanaka)) × ‘Trovita’ orange) × ‘Kankitsu Chukanbohon Nou 5 gou’ (‘Lee’ (Citrus clementina × tangelo) × Citrus kinokuni). The markers were integrated using common STSs on different phase maps in cross-pollination mode. The integrated map (AGI map) comprised 706 loci, including two morphological traits, and spanned 990.9 centimorgans (cM) with an average marker distance of 1.40 cM. These markers formed nine linkage groups (LGs) (corresponding to citrus physical chromosomes): LG-01 to LG-09 corresponded to Scaffold_01, Scaffold_07, Scaffold_09, Scaffold_06, Scaffold_03, Scaffold_02, Scaffold_04, Scaffold_08, and Scaffold_05, respectively. LG-08 and LG-09 contained morphological traits controlling embryo color and seedlessness. Eighty-eight loci comprised three or more alleles on the AGI map; 36.4 % of them were related to transcription factors and DNA-binding proteins. The 708 gene-based markers and the AGI map are valuable for integrating various citrus genetic maps, alignment of genomic sequences, chromosome assignment, and understanding the diversity of citrus germplasms.
Features
This publication contains information about 710 features:
Feature NameUniquenameType
Gn0054Gn0054genetic_marker
Gn0056Gn0056genetic_marker
Gn0058Gn0058genetic_marker
Gn0061Gn0061genetic_marker
Gn0063Gn0063genetic_marker
Gn0064Gn0064genetic_marker
Gn0065Gn0065genetic_marker
Gn0066Gn0066genetic_marker
Gn0067Gn0067genetic_marker
Gn0068Gn0068genetic_marker
Gn0069Gn0069genetic_marker
Gn0071Gn0071genetic_marker
Gn0073Gn0073genetic_marker
Gn0086Gn0086genetic_marker
Gn0097Gn0097genetic_marker
Gn0100Gn0100genetic_marker
Gn0101Gn0101genetic_marker
If0002If0002genetic_marker
If0003If0003genetic_marker
If0010If0010genetic_marker
If0203If0203genetic_marker
If0205If0205genetic_marker
If0207If0207genetic_marker
If0208If0208genetic_marker
If0210If0210genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Citrus-Okitsu-46-gou/Kankitsu-Chukanbohon-Nou-5-gou-F2-2014
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Okitsu-46-gouOkitsu-46-gouaccession
Kankitsu-Chukanbohon-Nou-5-gouKankitsu-Chukanbohon-Nou-5-gouaccession
Citrus-Okitsu-46-gou/Kankitsu-Chukanbohon-Nou-5-gou-F2Citrus-Okitsu-46-gou/Kankitsu-Chukanbohon-Nou-5-gou-F2population
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2014
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s11295-014-0738-9
KeywordsCitrus clementina, Citrus hassaku, Citrus unshiu, DNA-binding proteins, alleles, clementines, color, cross pollination, exons, germplasm, introns, linkage groups, loci, polymerase chain reaction, seedless varieties, tangelos, transcription factors