Endophytic Methylobacterium extorquens expresses a heterologous β-1,4-endoglucanase A (EglA) in Catharanthus roseus seedlings, a model host plant for Xylella fastidiosa

Publication Overview
TitleEndophytic Methylobacterium extorquens expresses a heterologous β-1,4-endoglucanase A (EglA) in Catharanthus roseus seedlings, a model host plant for Xylella fastidiosa
AuthorsFerreira Filho AS, Quecine MC, Bogas AC, Rossetto PdB, Lima AOdS, Lacava PT, Azevedo JL, Araújo WL
TypeJournal Article
Journal NameWorld journal of microbiology and biotechnology
Volume28
Issue4
Year2012
Page(s)1475-1481
CitationFerreira Filho AS, Quecine MC, Bogas AC, Rossetto PdB, Lima AOdS, Lacava PT, Azevedo JL, Araújo WL. Endophytic Methylobacterium extorquens expresses a heterologous β-1,4-endoglucanase A (EglA) in Catharanthus roseus seedlings, a model host plant for Xylella fastidiosa. World journal of microbiology and biotechnology. 2012; 28(4):1475-1481.

Abstract

Based on the premise of symbiotic control, we genetically modified the citrus endophytic bacterium Methylobacterium extorquens, strain AR1.6/2, and evaluated its capacity to colonize a model plant and its interaction with Xylella fastidiosa, the causative agent of Citrus Variegated Chlorosis (CVC). AR1.6/2 was genetically transformed to express heterologous GFP (Green Fluorescent Protein) and an endoglucanase A (EglA), generating the strains ARGFP and AREglA, respectively. By fluorescence microscopy, it was shown that ARGFP was able to colonize xylem vessels of the Catharanthus roseus seedlings. Using scanning electron microscopy, it was observed that AREglA and X. fastidiosa may co-inhabit the C. roseus vessels. M. extorquens was observed in the xylem with the phytopathogen X. fastidiosa, and appeared to cause a decrease in biofilm formation. AREglA stimulated the production of resistance protein, catalase, in the inoculated plants. This paper reports the successful transformation of AR1.6/2 to generate two different strains with a different gene each, and also indicates that AREglA and X. fastidiosa could interact inside the host plant, suggesting a possible strategy for the symbiotic control of CVC disease. Our results provide an enhanced understanding of the M. extorquens—X. fastidiosa interaction, suggesting the application of AR1.6/2 as an agent of symbiotic control.
Stocks
This publication contains information about 5 stocks:
Stock NameUniquenameType
Buddha's HandBuddha's Handcultivar
CleopatraCleopatracultivar
CorsicanCorsicancultivar
PinkPinkcultivar
Willow leafWillow leafcultivar
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2012
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s11274-011-0949-2
KeywordsCatharanthus roseus, Citrus, Methylobacterium extorquens, Xylella fastidiosa, bacteria, catalase, citrus variegated chlorosis, endophytes, fluorescence microscopy, genes, green fluorescent protein, host plants, scanning electron microscopy, seedlings, xylem vessels
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4746292
PMID: PubMedPMID:22805929