Development of indel markers from Citrus clementina (Rutaceae) BAC-end sequences and interspecific transferability in Citrus

Publication Overview
TitleDevelopment of indel markers from Citrus clementina (Rutaceae) BAC-end sequences and interspecific transferability in Citrus
AuthorsOllitrault F, Terol J, Martin AA, Pina JA, Navarro L, Talon M, Ollitrault P
TypeJournal Article
Journal NameAmerican Journal of Botany
Volume99
Issue7
Year2012
Page(s)e268-e273
CitationOllitrault F, Terol J, Martin AA, Pina JA, Navarro L, Talon M, and Ollitrault P. Development of indel markers from Citrus clementina (Rutaceae) BAC-end sequences and interspecific transferability in Citrus. American Journal of Botany. 2012. 99:e268-e273.

Abstract

PREMISE OF THE STUDY: Indel markers were developed from BAC-end sequences of Citrus clementina cv. Nules. Transferability and polymorphism were tested in the Citrus genus to estimate the potential of indel markers mined from a single genotype for use in genetic studies. METHODS AND RESULTS: Using polyacrylamide gel electrophoresis and DNA silver staining, 89 indel markers were tested for their transferability and polymorphism. Thirty-eight markers were selected. Heterozygosity in C. clementina cv. Nules was confirmed for 33 of these indel pairs. A preliminary diversity study using a capillary electrophoresis fragment analyzer was conducted with 21 indels using 45 accessions representing Citrus genus diversity. Intraspecific and interspecific polymorphisms were observed. CONCLUSIONS: These results indicate the utility of indel markers developed from sequence data of a single genotype of interspecific origin. In Citrus, these markers will be useful for genetic mapping, germplasm characterization, and phylogenetic assignment of DNA fragments.
Features
This publication contains information about 38 features:
Feature NameUniquenameType
CID0204CID0204genetic_marker
CID0502CID0502genetic_marker
CID0591CID0591genetic_marker
CID0593CID0593genetic_marker
CID0599CID0599genetic_marker
CID0806CID0806genetic_marker
CID0959CID0959genetic_marker
CID1553CID1553genetic_marker
CID1735CID1735genetic_marker
CID1862CID1862genetic_marker
CID2401CID2401genetic_marker
CID2493CID2493genetic_marker
CID2540CID2540genetic_marker
CID2590CID2590genetic_marker
CID2788CID2788genetic_marker
CID2945CID2945genetic_marker
CID3524CID3524genetic_marker
CID3541CID3541genetic_marker
CID4122CID4122genetic_marker
CID4210CID4210genetic_marker
CID4583CID4583genetic_marker
CID4721CID4721genetic_marker
CID4877CID4877genetic_marker
CID4894CID4894genetic_marker
CID5362CID5362genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Journal AbbreviationAm J Bot
URLhttp://www.amjbot.org/content/99/7/e268.full?sid=679cd9db-1f11-444f-a6ea-156b8749e8ce