A new strategy employed for identification of sweet orange cultivars with RAPD markers

Publication Overview
TitleA new strategy employed for identification of sweet orange cultivars with RAPD markers
AuthorsSun X, Mu Q, Jiang D, Wang C, Wang XC, Fang JG
TypeJournal Article
Journal NameGenetics and molecular research : GMR
Volume11
Issue3
Year2012
Page(s)2071-80
CitationSun X, Mu Q, Jiang D, Wang C, Wang XC, Fang JG. A new strategy employed for identification of sweet orange cultivars with RAPD markers. Genetics and molecular research : GMR. 2012; 11(3):2071-80.

Abstract

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar identification. Thirteen effective primers were first screened from 54 RAPD arbitrary 11-mer primers based on their amplification products and amplified polymorphic bands; they were then used for PCR amplification of all 60 cultivars. All cultivars were manually and completely separated by the polymorphic bands appearing in DNA fingerprints from 13 primers; a CID of the 60 sweet orange cultivars was then constructed. This CID separated all the cultivars from each other, based on the polymorphic bands; the corresponding primers were marked in the correct positions on the sweet orange CID. The CID strategy facilitates the identification of fruit cultivars with DNA markers. This CID of sweet orange cultivars will be very useful for the protection of cultivar rights and for early identification of seedlings in the nursery industry.

Stocks
This publication contains information about 59 stocks:
Stock NameUniquenameType
Anjiangdahongcheng 1haoAnjiangdahongcheng 1haocultivar
Anyuan C3Anyuan C3cultivar
Cadenera Sweet OrangeCadenera Sweet Orangecultivar
Cara red flesh navel orangeCara red flesh navel orangecultivar
Chengxi late-maturingChengxi late-maturingcultivar
Cuba taoyechengCuba taoyechengcultivar
DeltaDeltacultivar
Egypt honey orangeEgypt honey orangecultivar
Eight lanes tianchengEight lanes tianchengcultivar
FengqiFengqicultivar
Fukumoto Navel OrangeFukumoto Navel Orangecultivar
GuangxixiangshuichengGuangxixiangshuichengcultivar
HamlinHamlincultivar
Hanyuan seedlessHanyuan seedlesscultivar
HongmaochengHongmaochengcultivar
HuangbaipitianchengHuangbaipitianchengcultivar
Huapeng 9-11Huapeng 9-11cultivar
Ichang papedaIchang papedacultivar
Italy hamlinItaly hamlincultivar
JiangbeimeiganJiangbeimeigancultivar
JintangchengJintangchengcultivar
Kaixian seedlessKaixian seedlesscultivar
KaixianbopiKaixianbopicultivar
Late-maturing navel orangeLate-maturing navel orangecultivar
LingyangshaohedahongtianchengLingyangshaohedahongtianchengcultivar

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic
ISSN1676-5680
eISSN1676-5680
Publication Date2012
Journal AbbreviationGenet. Mol. Res.
DOI10.4238/2012.August.6.11
Elocation10.4238/2012.August.6.11
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryBrazil
Cross References
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DatabaseAccession
PMID: PubMedPMID:22911591