FC868662

Overview
NameFC868662
Unique NameFC868662
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length736
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC868662_ssr372 microsatellite FC868662_ssr372:372..389. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig9374 contig Ccv1_Contig9374:1..737. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 1.849e-24
Identity = 49/108 (45.37%), Postives = 65/108 (60.19%), Query Frame = 3 
Query:  321 LLAAICMWVCPCILASMXXXXXXSHGLLRIQLKKRQLGINTINAARLITKNEVHNRFNHPKADVVYLNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLF 644
            LL   C+W   C L         S G LR+ L K++L    + AA+L  +     +     +D V L +YL+ QYYG IG+GSPPQ+F+V+FDTGSSNLWVPS+KC F
Sbjct:    7 LLVTTCLWALSCALL----LHASSDGFLRVNLNKKRLDKEDLTAAKLAQQGNRLLKTGSSDSDPVPLVDYLNTQYYGVIGLGSPPQNFTVIFDTGSSNLWVPSAKCYF 110

HSP 2 Score: 36.5798 bits (83), Expect = 1.849e-24
Identity = 15/27 (55.56%), Postives = 19/27 (70.37%), Query Frame = 2 
Query:  641 FSISCYLHSRYRARLSRTYTKIGVPCK 721
            FSI+CYLHSRY ++ S +Y   G  CK
Sbjct:  110 FSIACYLHSRYNSKKSSSYKADGETCK 136
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.978e-23
Identity = 47/111 (42.34%), Postives = 68/111 (61.26%), Query Frame = 3 
Query:  330 AICMWVCPCILASMXXXXXXSHGLLRIQLKKRQLGINTINAARLITKNE------VHNRFNHPKADVVYLNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLF 644
            A+ +     +L ++      + GL+RI LKKR +  N+  A  L    E       +   +  + D+V L NY++AQY+GEIG+G+PPQ F+V+FDTGSSNLWVPS+KC F
Sbjct:    7 ALALLAAVLLLQTVLPAASEAEGLVRIALKKRPIDRNSRVATGLSGGEEQPLLSGANPLRSEEEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYF 117

HSP 2 Score: 38.891 bits (89), Expect = 1.978e-23
Identity = 17/25 (68.00%), Postives = 19/25 (76.00%), Query Frame = 2 
Query:  641 FSISCYLHSRYRARLSRTYTKIGVP 715
            FSI+CYLHSRY+A  S TY K G P
Sbjct:  117 FSIACYLHSRYKAGASSTYKKNGKP 141
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 90.8929 bits (224), Expect = 6.054e-22
Identity = 50/115 (43.48%), Postives = 69/115 (60.00%), Query Frame = 3 
Query:  330 AICMWVCPCILASMXXXXXXSHGLLRIQLKKRQLGINTINAARLITKNEVHNRFNHPKA----------DVVYLNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLF 644
            ++ + +   +L         + GL+RI LKKR +  N+  AARL +  E   R     A          D+V L NY++AQY+GEIG+G+PPQ F+V+FDTGSSNLWVPS+KC F
Sbjct:    5 SVALVLLAAVLLQALLPASAAEGLVRIALKKRPIDENSRVAARL-SGEEGARRLGLRGANSLGGGGGEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYF 118

HSP 2 Score: 34.2686 bits (77), Expect = 6.054e-22
Identity = 14/25 (56.00%), Postives = 18/25 (72.00%), Query Frame = 2 
Query:  641 FSISCYLHSRYRARLSRTYTKIGVP 715
            FSI+C+ HSRY++  S TY K G P
Sbjct:  118 FSIACFFHSRYKSGQSSTYQKNGKP 142
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 3.133e-20
Identity = 42/77 (54.55%), Postives = 52/77 (67.53%), Query Frame = 3 
Query:  432 GINTINAARLITKNEVHNRFNHPKADVVYLNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLFLYLAIY 662
            G N  NA R   K  V   F     +++ L NY+DAQY+GEIGIG+PPQ F+V+FDTGSSNLWVPSSKC F    ++
Sbjct:   17 GSNDANARR---KYGVRGNFRDSDGELIALKNYMDAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCYFSVACLF 90

HSP 2 Score: 30.0314 bits (66), Expect = 3.133e-20
Identity = 12/23 (52.17%), Postives = 16/23 (69.57%), Query Frame = 2 
Query:  641 FSISCYLHSRYRARLSRTYTKIG 709
            FS++C  HS+YR+  S TY K G
Sbjct:   84 FSVACLFHSKYRSTDSTTYKKNG 106
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.601e-18
Identity = 53/114 (46.49%), Postives = 69/114 (60.53%), Query Frame = 3 
Query:  342 WVCPCILASMXXXXXXSH-GLLRIQLKKRQLGINTINAARLITKN----EVHNRFNHPKA--------DVVYLNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLF 644
            ++C  +L S       S+ GLLR+ LKK +L      AAR+ +K+    +   R  +PK         D+V L NYLDAQYYGEI IG+PPQ F+V+FDTGSSNLWV   +CLF
Sbjct:   10 FLCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWV-LCECLF 122

HSP 2 Score: 29.261 bits (64), Expect = 4.601e-18
Identity = 10/23 (43.48%), Postives = 17/23 (73.91%), Query Frame = 2 
Query:  641 FSISCYLHSRYRARLSRTYTKIG 709
            FS++C+ H+RY++  S +Y K G
Sbjct:  122 FSVACHFHARYKSSRSSSYKKNG 144
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: RENI_CALJA (Renin OS=Callithrix jacchus GN=REN PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.451e-16
Identity = 49/118 (41.53%), Postives = 63/118 (53.39%), Query Frame = 3 
Query:  303 RMGIKFLLAAICMWVCPCILASMXXXXXXSHGLLRIQLKKRQLGINTINAARLITKNEVHNRFNHPKADVVYLNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLFLYLA 656
            R G+  LL   C +  P    +           +R  LK+R + +  +   R+   N   +         V L NY+D QYYGEIGIG+PPQ+F VVFDTGSSN+WVPSSKC  LY A
Sbjct:    9 RWGLLLLLWGSCTFGLPTETTTFKRISLKRMPSIRESLKERGVDMARLGPERMALVNITSS---------VILTNYMDTQYYGEIGIGTPPQTFKVVFDTGSSNVWVPSSKCSRLYTA 117
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: CATD_HUMAN (Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.999e-16
Identity = 36/49 (73.47%), Postives = 41/49 (83.67%), Query Frame = 3 
Query:  519 LNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLFLYLAIYI 665
            L NY+DAQYYGEIGIG+PPQ F+VVFDTGSSNLWVPS  C  L +A +I
Sbjct:   71 LKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWI 119

HSP 2 Score: 25.409 bits (54), Expect = 3.999e-16
Identity = 9/21 (42.86%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  647 ISCYLHSRYRARLSRTYTKIG 709
            I+C++H +Y +  S TY K G
Sbjct:  115 IACWIHHKYNSDKSSTYVKNG 135
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: CATD_CANFA (Cathepsin D OS=Canis familiaris GN=CTSD PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.999e-16
Identity = 36/49 (73.47%), Postives = 41/49 (83.67%), Query Frame = 3 
Query:  519 LNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLFLYLAIYI 665
            L NY+DAQYYGEIGIG+PPQ F+VVFDTGSSNLWVPS  C  L +A +I
Sbjct:   71 LRNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWI 119

HSP 2 Score: 25.409 bits (54), Expect = 3.999e-16
Identity = 9/21 (42.86%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  647 ISCYLHSRYRARLSRTYTKIG 709
            I+C++H +Y +  S TY K G
Sbjct:  115 IACWIHHKYNSGKSSTYVKNG 135
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: CATD_RAT (Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 4.000e-16
Identity = 36/49 (73.47%), Postives = 41/49 (83.67%), Query Frame = 3 
Query:  519 LNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLFLYLAIYI 665
            L NYLDAQYYGEIGIG+PPQ F+VVFDTGSSNLWVPS  C  L +A ++
Sbjct:   71 LKNYLDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWV 119

HSP 2 Score: 25.0238 bits (53), Expect = 4.000e-16
Identity = 9/21 (42.86%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  647 ISCYLHSRYRARLSRTYTKIG 709
            I+C++H +Y +  S TY K G
Sbjct:  115 IACWVHHKYNSDKSSTYVKNG 135
[Close]
BLAST of FC868662 vs. ExPASy Swiss-Prot
Match: CATD_PIG (Cathepsin D OS=Sus scrofa GN=CTSD PE=1 SV=2)

HSP 1 Score: 80.1073 bits (196), Expect = 4.017e-16
Identity = 36/49 (73.47%), Postives = 41/49 (83.67%), Query Frame = 3 
Query:  519 LNNYLDAQYYGEIGIGSPPQSFSVVFDTGSSNLWVPSSKCLFLYLAIYI 665
            L NY+DAQYYGEIGIG+PPQ F+VVFDTGSSNLWVPS  C  L +A +I
Sbjct:    7 LKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWI 55

HSP 2 Score: 25.409 bits (54), Expect = 4.017e-16
Identity = 9/21 (42.86%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  647 ISCYLHSRYRARLSRTYTKIG 709
            I+C++H +Y +  S TY K G
Sbjct:   51 IACWIHHKYNSGKSSTYVKNG 71
The following BLAST results are available for this feature:
BLAST of FC868662 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 65
Match NameE-valueIdentityDescription
ASPRX_ORYSJ1.849e-2445.37Aspartic proteinase OS=Oryza sativa subsp. japonic... [more]
ASPR_HORVU1.978e-2342.34Phytepsin OS=Hordeum vulgare PE=1 SV=1[more]
ASPR1_ORYSJ6.054e-2243.48Aspartic proteinase oryzasin-1 OS=Oryza sativa sub... [more]
CYPR1_CYNCA3.133e-2054.55Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO... [more]
ASPR_CUCPE4.601e-1846.49Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1[more]
RENI_CALJA1.451e-1641.53Renin OS=Callithrix jacchus GN=REN PE=2 SV=1[more]
CATD_HUMAN3.999e-1673.47Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1[more]
CATD_CANFA3.999e-1673.47Cathepsin D OS=Canis familiaris GN=CTSD PE=2 SV=1[more]
CATD_RAT4.000e-1673.47Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1[more]
CATD_PIG4.017e-1673.47Cathepsin D OS=Sus scrofa GN=CTSD PE=1 SV=2[more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC31003B12EF AbioticL1 Citrus clementina cDNA clone C31003B12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC868662 ID=FC868662; Name=FC868662; organism=Citrus clementina; type=EST; length=736bp
GATTACGAAACCACGGTAGTGAGACTATTCACTGAAACAAATAAATAAAC
TTACTTGTTCAATAATTAATCTATCTAAACTATAAACGAATAAAATTATT
TTGTCAATGACTCACTGATCTCCAAGTACAACGCGGGAAGAGAAATTGCG
CAGTGGTTTCCTGAGCATTTAATCACACCCACACGAAACAAGCTCACAGA
TGATTCACTACAGGGTGCAAACATAAAAAGGCAGGCCAGGCAGCTCGTTG
GTTCGTTCGTTGATTGGCGCCATATCATCAACTCAGCAGGAAAATTTGTG
TAAGAATGGGGATTAAGTTTCTTCTTGCTGCAATATGTATGTGGGTTTGC
CCCTGCATCTTAGCCTCGATGCCTCCTCCTCCTCCTCCTTCTCATGGGTT
ACTGAGGATTCAACTGAAGAAGCGGCAGTTAGGCATTAACACCATCAACG
CTGCAAGGTTAATCACTAAAAACGAGGTTCACAATCGTTTCAATCATCCT
AAAGCAGATGTAGTTTATTTAAACAACTATCTTGATGCCCAATATTATGG
AGAGATTGGTATTGGTTCACCTCCACAAAGTTTCTCTGTCGTCTTTGATA
CTGGCAGCTCTAATCTTTGGGTCCCATCTTCTAAATGCCTTTTTCTATAT
CTTGCTATTTACATTCGAGATACAGGGCGAGGCTGTCCAGGACTTATACT
AAGATTGGAGTACCTTGCAAATTCATTATGGCTCTG
back to top