Proteomic analysis of the Mexican lime tree response to "Candidatus Phytoplasma aurantifolia" infection

Publication Overview
TitleProteomic analysis of the Mexican lime tree response to "Candidatus Phytoplasma aurantifolia" infection
AuthorsTaheri F, Nematzadeh G, Zamharir MG, Nekouei MK, Naghavi M, Mardi M, Salekdeh GH
TypeJournal Article
Journal NameMolecular bioSystems
Volume7
Issue11
Year2011
Page(s)3028-35
CitationTaheri F, Nematzadeh G, Zamharir MG, Nekouei MK, Naghavi M, Mardi M, Salekdeh GH. Proteomic analysis of the Mexican lime tree response to "Candidatus Phytoplasma aurantifolia" infection. Molecular bioSystems. 2011 Nov; 7(11):3028-35.

Abstract

"Candidatus Phytoplasma aurantifolia" is the causative agent of witches' broom disease in the Mexican lime tree (Citrus aurantifolia L.), and is responsible for major tree losses in Southern Iran and Oman. The pathogen is strictly biotrophic, and, therefore, completely dependent on living host cells for its survival. The molecular basis of compatibility and disease development in this system is poorly understood. We applied a proteomics approach to analyse gene expression in Mexican limes infected with "Ca. Phytoplasma aurantifolia". Leaf samples were collected from healthy and infected plants and were analysed using 2-DE coupled with MS. Among 800 leaf proteins that were detected reproducibly in eight biological replicates of healthy and eight biological replicates of infected plants, 55 showed a significant response to the disease. MS resulted in identification of 39 regulated proteins, which included proteins that were involved in oxidative stress defence, photosynthesis, metabolism, and the stress response. Our results provide the first proteomic view of the molecular basis of the infection process and identify genes that could help inhibit the effects of the pathogen.

Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
Mexican limeMexican limebreeding_research_material
Features
This publication contains information about 7 features:
Feature NameUniquenameType
6-PGL6-PGLgenetic_marker
AP2.1AP2.1genetic_marker
Cu/ZnSDCu/ZnSDgenetic_marker
GSTOGSTOgenetic_marker
MP1MP1genetic_marker
PRP10.6PRP10.6genetic_marker
TSTTSTgenetic_marker
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Property NameValue
CopyrightThis journal is © The Royal Society of Chemistry 2011
Publication ModelPrint-Electronic
ISSN1742-2051
eISSN1742-2051
Publication Date2011 Nov
Journal AbbreviationMol Biosyst
DOI10.1039/c1mb05268c
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Elocation10.1039/c1mb05268c
Journal CountryEngland
Publication TypeResearch Support, Non-U.S. Gov't
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DatabaseAccession
PMID: PubMedPMID:21853195