An arginine decarboxylase gene PtADC from Poncirus trifoliata confers abiotic stress tolerance and promotes primary root growth in Arabidopsis

Publication Overview
TitleAn arginine decarboxylase gene PtADC from Poncirus trifoliata confers abiotic stress tolerance and promotes primary root growth in Arabidopsis
AuthorsWang J, Sun P, Chen C, Wang Y, Fu X, Liu J
TypeJournal Article
Journal NameJournal of experimental botany
Volume62
Issue8
Year2011
Page(s)2899-2914
CitationWang J, Sun P, Chen C, Wang Y, Fu X, Liu J. An arginine decarboxylase gene PtADC from Poncirus trifoliata confers abiotic stress tolerance and promotes primary root growth in Arabidopsis. Journal of experimental botany. 2011; 62(8):2899-2914.

Abstract

Arginine decarboxylase (ADC) is an important enzyme responsible for polyamine synthesis under stress conditions. In this study, the gene encoding ADC in Poncirus trifoliata (PtADC) was isolated and it existed as a single-copy member. Transcript levels of PtADC were up-regulated by low temperature and dehydration. Overexpression of PtADC in an Arabidopsis thaliana ADC mutant adc1-1 promoted putrescine synthesis in the transgenic line and the stomatal density was reverted to that in the wild type. The transgenic line showed enhanced resistance to high osmoticum, dehydration, long-term drought, and cold stress compared with the wild type and the mutant. The accumulation of reactive oxygen species (ROS) in the transgenic line was appreciably decreased under the stresses, but ROS scavenging capacity was compromised when the transgenic plants were treated with the ADC inhibitor D-arginine prior to stress treatment. In addition, the transgenic line had longer roots than the wild type and the mutant under both normal and stressful conditions, consistent with larger cell number and length of the root meristematic zone. Taken together, these results demonstrated that PtADC is involved in tolerance to multiple stresses, and its function may be due, at least partly, to efficient ROS elimination and to its influence on root growth conducive to drought tolerance.
Features
This publication contains information about 12 features:
Feature NameUniquenameType
ADC1ADC1genetic_marker
ADC2ADC2genetic_marker
ADC3ADC3genetic_marker
ADC4ADC4genetic_marker
Actin.2Actin.2genetic_marker
NPTNPTgenetic_marker
35S-ADC235S-ADC2genetic_marker
SDD1SDD1genetic_marker
YDAYDAgenetic_marker
CYCB1;1CYCB1;1genetic_marker
TubulinTubulingenetic_marker
HQ008237HQ008237.1region
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
Trifoliate orangeTrifoliate orangeaccession
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2011
Published Location|||
Language Abbreng
KeywordsArabidopsis thaliana, Poncirus trifoliata, arginine decarboxylase, cold stress, drought, drought tolerance, genes, mutants, reactive oxygen species, rhizosphere, root growth, roots, stress tolerance, transgenic plants