Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers

Publication Overview
TitleComparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers
AuthorsBiswas MK, Chai L, Amar MH, Zhang X, Deng X
TypeJournal Article
Journal NameScientia horticulturae
Volume129
Issue4
Year2011
Page(s)798-803
CitationBiswas MK, Chai L, Amar MH, Zhang X, Deng X. Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers. Scientia horticulturae. 2011; 129(4):798-803.

Abstract

In this study we evaluate the informativeness and efficiency of Amplified Fragment Length Polymorphism (AFLP), Sequence-Specific Amplified Polymorphism (S-SAP), Selectively Amplified Microsatellite Polymorphic Loci (SAMPL) and Simple Sequence Repeat (SSR) markers for genetic diversity, phylogenetic relationship among the Citrus species and mapping ability of the marker system. The SSR exhibited relatively higher level of polymorphism information content in terms of the expected heterozygosity, than that of the AFLPs, SSAPs and SAMPLs. For each marker system, average level of the discriminating potential was very close to the actual discriminating potential. Similarity matrices showed weak, yet significant correlations when Mantel's test was applied. The highest positive (0.72) correlation was found between the AFLP and SSAP markers. The SSR and SAMPL markers were poorly correlated. The dendrogram topology among the four marker systems had high similarity. Taken together, the SSAP and SAMPL were highly efficient in detecting genetic similarity in Citrus, while the SSR may be more useful for segregation studies and genome mapping in Citrus. The SSAP and SAMPL markers could be useful for Citrus genome mapping in combination with AFLP and SSR markers. To our knowledge, this was the first detail report of a comparison of performances among AFLP, SSR and retrotrasposon based molecular marker technique on a set of samples of Citrus. Our result provides guidance for future efficient use of these molecular methods in genetic analysis of Citrus sp. and its relatives.
Features
This publication contains information about 4 features:
Feature NameUniquenameType
LTR1LTR1genetic_marker
LTR2LTR2genetic_marker
LTR3LTR3genetic_marker
LTR4LTR4genetic_marker
Stocks
This publication contains information about 33 stocks:
Stock NameUniquenameType
Chinese LimonChinese Limoncultivar
EurekaEurekacultivar
Luo GangLuo Gangcultivar
Mexican limeMexican limebreeding_research_material
MurcottMurcottcultivar
NewhallNewhallcultivar
NovaNovacultivar
Xin huiXin huicultivar
BendizaojuBendizaojucultivar
Cara CaraCara Caracultivar
ClementineClementinecultivar
DaidaiDaidaiwild_unimproved
Fingered CitronFingered Citroncultivar
Goutou sour orangeGoutou sour orangecultivar
Guoqin No 4Guoqin No 4cultivar
HamlinHamlincultivar
HonjuHonjucultivar
Ichang papedaIchang papedacultivar
JinchengJinchengcultivar
KuikanKuikancultivar
MashuijuMashuijucultivar
MiyagawaMiyagawacultivar
NanfengNanfengcultivar
QingpiQingpicultivar
Red marshRed marshcultivar

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2011
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1016/j.scienta.2011.06.015
KeywordsCitrus, amplified fragment length polymorphism, genetic markers, genetic variation, germplasm conservation, microsatellite repeats, phylogeny
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4602307