Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers

Publication Overview
TitleComparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers
AuthorsBiswas MK, Chai L, Amar MH, Zhang X, Deng X
TypeJournal Article
Journal NameScientia horticulturae
Volume129
Issue4
Year2011
Page(s)798-803
CitationBiswas MK, Chai L, Amar MH, Zhang X, Deng X. Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers. Scientia horticulturae. 2011; 129(4):798-803.

Abstract

In this study we evaluate the informativeness and efficiency of Amplified Fragment Length Polymorphism (AFLP), Sequence-Specific Amplified Polymorphism (S-SAP), Selectively Amplified Microsatellite Polymorphic Loci (SAMPL) and Simple Sequence Repeat (SSR) markers for genetic diversity, phylogenetic relationship among the Citrus species and mapping ability of the marker system. The SSR exhibited relatively higher level of polymorphism information content in terms of the expected heterozygosity, than that of the AFLPs, SSAPs and SAMPLs. For each marker system, average level of the discriminating potential was very close to the actual discriminating potential. Similarity matrices showed weak, yet significant correlations when Mantel's test was applied. The highest positive (0.72) correlation was found between the AFLP and SSAP markers. The SSR and SAMPL markers were poorly correlated. The dendrogram topology among the four marker systems had high similarity. Taken together, the SSAP and SAMPL were highly efficient in detecting genetic similarity in Citrus, while the SSR may be more useful for segregation studies and genome mapping in Citrus. The SSAP and SAMPL markers could be useful for Citrus genome mapping in combination with AFLP and SSR markers. To our knowledge, this was the first detail report of a comparison of performances among AFLP, SSR and retrotrasposon based molecular marker technique on a set of samples of Citrus. Our result provides guidance for future efficient use of these molecular methods in genetic analysis of Citrus sp. and its relatives.
Features
This publication contains information about 4 features:
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LTR3LTR3genetic_marker
LTR4LTR4genetic_marker
Stocks
This publication contains information about 33 stocks:
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Chinese LimonChinese Limonaccession
EurekaEurekaaccession
Luo GangLuo Gangaccession
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DaidaiDaidaiaccession
Fingered CitronFingered Citronaccession
Goutou sour orangeGoutou sour orangeaccession
Guoqin No 4Guoqin No 4accession
HamlinHamlinaccession
HonjuHonjuaccession
Ichang papedaIchang papedaaccession
JinchengJinchengaccession
KuikanKuikanaccession
MashuijuMashuijuaccession
MiyagawaMiyagawaaccession
NanfengNanfengaccession
QingpiQingpiaccession
Red marshRed marshaccession

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Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2011
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1016/j.scienta.2011.06.015
KeywordsCitrus, amplified fragment length polymorphism, genetic markers, genetic variation, germplasm conservation, microsatellite repeats, phylogeny