Isolation of a Polygalacturonase Gene from Citrus sinensis Fruit and Its Expression Relative to Fruit Mastication Trait, Fruit Development, and Calcium or Boron Treatments

Publication Overview
TitleIsolation of a Polygalacturonase Gene from Citrus sinensis Fruit and Its Expression Relative to Fruit Mastication Trait, Fruit Development, and Calcium or Boron Treatments
AuthorsLiu Y, Dong T, Lei Y, Deng X, Gu Q
TypeJournal Article
Journal NamePlant molecular biology reporter
Volume29
Issue1
Year2011
Page(s)51-59
CitationLiu Y, Dong T, Lei Y, Deng X, Gu Q. Isolation of a Polygalacturonase Gene from Citrus sinensis Fruit and Its Expression Relative to Fruit Mastication Trait, Fruit Development, and Calcium or Boron Treatments. Plant molecular biology reporter. 2011; 29(1):51-59.

Abstract

Polygalacturonase (PG) plays a significant role in pectin solubilization and depolymerization in vivo and is involved in numerous plant developmental processes. In this study, a polygalacturonase gene (CitPG, EF185420) was isolated from Citrus sinensis. It is 1,686 bp with a putative open reading frame of 1,338 bp encoding 445 amino acids. Phylogenetic analysis showed that CitPG belongs to clade B which includes PGs expressed in fruits or dehiscence zones. CitPG expression profiles during fruit ripening were compared between two pairs of varieties which differ in their mastication traits: C. sinensis cv. Fengjiewancheng (FJWC) vs. C. sinensis cv. Fengjie 72-1(FJ72-1) and Citrus reticulata cv. Kinokuni (NM) vs. C. reticulata cv. Miguang (MG), in which the mastication trait of “FJWC” and “NM” are inferior to their respective partner cultivars. Results indicated that the CitPG transcript level was significantly lower in the inferior mastication variety at the late ripening stage or at all ripening stages. Calcium or boron treatment resulted in a significant reduction in CitPG transcription level in fruit pulp at harvest time (235 days after anthesis). This was accompanied by an increase in protopectin content. In addition, the mRNA level of CitPG in the fruit peel and pulp increased during the fruit's rapid cell enlargement stage and was inversely correlated with protopectin levels. These results suggest that CitPG is associated with the favorable enhancement of the fruit mastication trait and is involved in dissolving the protopectin during cell enlargement.
Stocks
This publication contains information about 5 stocks:
Stock NameUniquenameType
Fengjie 72-1Fengjie 72-1accession
FengjiewanchengFengjiewanchengaccession
MiguangMiguangaccession
NanfengmijuNanfengmijuaccession
Cara CaraCara Caraaccession
Features
This publication contains information about 9 features:
Feature NameUniquenameType
CitPGCitPGgenetic_marker
L1L1genetic_marker
L2L2genetic_marker
R1R1genetic_marker
R2R2genetic_marker
PGLPGLgenetic_marker
PGRPGRgenetic_marker
AlAlgenetic_marker
ArArgenetic_marker
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2011
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s11105-010-0206-0
KeywordsCitrus reticulata, Citrus sinensis, amino acids, boron, calcium, cell growth, cultivars, dehiscence, depolymerization, fruit peels, fruit pulp, fruiting, fruits, genes, harvest date, mastication, messenger RNA, open reading frames, pectins, phylogeny, polygalacturonase, ripening, transcription (genetics)
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4488532