FC919330

Overview
NameFC919330
Unique NameFC919330
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length588
Libraries
Library NameType
PCRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC919330 vs. ExPASy Swiss-Prot
Match: POL_CERV (Enzymatic polyprotein OS=Carnation etched ring virus GN=ORF V PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.849e-17
Identity = 39/108 (36.11%), Postives = 60/108 (55.56%), Query Frame = -3 
Query:  245 WTDEHTKTVRLIKNSVKCIPCLYLANPTLPKIVETDASDLGYGGILKQKENDKEQIIQYVSAHWNDCQKNYSTIKKEILSIVLCISKFQSDLLNQKFLLRIDCKAAKH 568
            W D  ++ +  IK ++K  P LY   P    ++ETDAS+  +GGILK   N  E I +Y S  +   ++NY + +KE+L+++  I KF   L   +FL+R D K   H
Sbjct:  500 WNDTDSQYMAKIKKNLKSFPKLYHPEPNDKLVIETDASEEFWGGILKAIHNSHEYICRYASGSFKAAERNYHSNEKELLAVIRVIKKFSIYLTPSRFLIRTDNKNFTH 607

HSP 2 Score: 33.113 bits (74), Expect = 2.849e-17
Identity = 17/34 (50.00%), Postives = 19/34 (55.88%), Query Frame = -2 
Query:   96 R*QAILSVFDFDIEFIKGDKNSIPDFLTREFLQN 197
            R Q  LS +DFD+E I G KN   DFL    L N
Sbjct:  624 RWQMWLSQYDFDVEHIAGTKNVFADFLQENTLTN 657
[Close]
BLAST of FC919330 vs. ExPASy Swiss-Prot
Match: POL_FMVD (Enzymatic polyprotein OS=Figwort mosaic virus (strain DxS) GN=ORF V PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.535e-14
Identity = 40/105 (38.10%), Postives = 59/105 (56.19%), Query Frame = -3 
Query:  257 WTDEHTKTVRLIKNSVKCIPCLYLANPTLPKIVETDASDLGYGGILKQKEND-KEQIIQYVSAHWNDCQKNYSTIKKEILSIVLCISKFQSDLLNQKFLLRIDCK 568
            WT   +  V+ IK ++   P LYL  P    I+ETDASD  +GG+LK +  D  E I +Y S  +   +KNY +  KE+L++   I+KF + L   +F +R D K
Sbjct:  509 WTQSDSDYVKKIKKNLGSFPKLYLPKPEDHLIIETDASDSFWGGVLKARALDGVELICRYSSGSFKQAEKNYHSNDKELLAVKQVITKFSAYLTPVRFTVRTDNK 613

HSP 2 Score: 30.4166 bits (67), Expect = 1.535e-14
Identity = 14/31 (45.16%), Postives = 20/31 (64.52%), Query Frame = -2 
Query:  105 R*QAILSVFDFDIEFIKGDKNSIPDFLTREF 197
            R Q   S + FD+E ++G KN + D LTR+F
Sbjct:  634 RWQNWFSKYQFDVEHLEGVKNVLADCLTRDF 664
[Close]
BLAST of FC919330 vs. ExPASy Swiss-Prot
Match: POL_CAMVS (Enzymatic polyprotein OS=Cauliflower mosaic virus (strain Strasbourg) GN=ORF V PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.993e-14
Identity = 38/109 (34.86%), Postives = 58/109 (53.21%), Query Frame = -3 
Query:  263 PAAWTDEHTKTVRLIKNSVKCIPCLYLANPTLPKIVETDASDLGYGGILK----QKENDKEQIIQYVSAHWNDCQKNYSTIKKEILSIVLCISKFQSDLLNQKFLLRID 577
            P  WT E T  ++ +K +++  P L+   P    I+ETDASD  +GG+LK     +  + E I +Y S  +   +KNY +  KE L+++  I KF   L    FL+R D
Sbjct:  513 PWRWTKEDTLYMQKVKKNLQGFPPLHHPLPEEKLIIETDASDDYWGGMLKAIKINEGTNTELICRYASGSFKAAEKNYHSNDKETLAVINTIKKFSIYLTPVHFLIRTD 621

HSP 2 Score: 36.965 bits (84), Expect = 1.993e-14
Identity = 18/31 (58.06%), Postives = 21/31 (67.74%), Query Frame = -2 
Query:  105 R*QAILSVFDFDIEFIKGDKNSIPDFLTREF 197
            R QA LS + FD+E IKG  N   DFL+REF
Sbjct:  644 RWQAWLSHYSFDVEHIKGTDNHFADFLSREF 674
[Close]
BLAST of FC919330 vs. ExPASy Swiss-Prot
Match: POL_CAMVD (Enzymatic polyprotein OS=Cauliflower mosaic virus (strain D/H) GN=ORF V PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.993e-14
Identity = 38/109 (34.86%), Postives = 58/109 (53.21%), Query Frame = -3 
Query:  263 PAAWTDEHTKTVRLIKNSVKCIPCLYLANPTLPKIVETDASDLGYGGILK----QKENDKEQIIQYVSAHWNDCQKNYSTIKKEILSIVLCISKFQSDLLNQKFLLRID 577
            P  WT E T  ++ +K +++  P L+   P    I+ETDASD  +GG+LK     +  + E I +Y S  +   +KNY +  KE L+++  I KF   L    FL+R D
Sbjct:  508 PWKWTKEDTLYMQKVKKNLQGFPPLHHPLPEEKLIIETDASDDYWGGMLKAIKINEGTNTELICRYASGSFKAAEKNYHSNDKETLAVINTIKKFSIYLTPVHFLIRTD 616

HSP 2 Score: 36.965 bits (84), Expect = 1.993e-14
Identity = 18/31 (58.06%), Postives = 21/31 (67.74%), Query Frame = -2 
Query:  105 R*QAILSVFDFDIEFIKGDKNSIPDFLTREF 197
            R QA LS + FD+E IKG  N   DFL+REF
Sbjct:  639 RWQAWLSHYSFDVEHIKGTDNHFADFLSREF 669
[Close]
BLAST of FC919330 vs. ExPASy Swiss-Prot
Match: POL_CAMVE (Enzymatic polyprotein OS=Cauliflower mosaic virus (strain BBC) GN=ORF V PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.370e-14
Identity = 37/109 (33.94%), Postives = 58/109 (53.21%), Query Frame = -3 
Query:  263 PAAWTDEHTKTVRLIKNSVKCIPCLYLANPTLPKIVETDASDLGYGGILK----QKENDKEQIIQYVSAHWNDCQKNYSTIKKEILSIVLCISKFQSDLLNQKFLLRID 577
            P  WT E T  ++ +K +++  P L+   P    I+ETDASD  +GG+LK     +  + E I +Y S  +   ++NY +  KE L+++  I KF   L    FL+R D
Sbjct:  513 PWKWTKEDTLYMQKVKKNLQGFPPLHHPLPEEKLIIETDASDDYWGGMLKAIKINEGTNTELICRYASGSFKAAERNYHSNDKETLAVINTIKKFSIYLTPVHFLIRTD 621

HSP 2 Score: 36.965 bits (84), Expect = 4.370e-14
Identity = 18/31 (58.06%), Postives = 21/31 (67.74%), Query Frame = -2 
Query:  105 R*QAILSVFDFDIEFIKGDKNSIPDFLTREF 197
            R QA LS + FD+E IKG  N   DFL+REF
Sbjct:  644 RWQAWLSHYSFDVEHIKGTDNHFADFLSREF 674
[Close]
BLAST of FC919330 vs. ExPASy Swiss-Prot
Match: POL_CAMVC (Enzymatic polyprotein OS=Cauliflower mosaic virus (strain CM-1841) GN=ORF V PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.370e-14
Identity = 37/109 (33.94%), Postives = 58/109 (53.21%), Query Frame = -3 
Query:  263 PAAWTDEHTKTVRLIKNSVKCIPCLYLANPTLPKIVETDASDLGYGGILK----QKENDKEQIIQYVSAHWNDCQKNYSTIKKEILSIVLCISKFQSDLLNQKFLLRID 577
            P  WT E T  ++ +K +++  P L+   P    I+ETDASD  +GG+LK     +  + E I +Y S  +   ++NY +  KE L+++  I KF   L    FL+R D
Sbjct:  513 PWKWTKEDTLYMQKVKKNLQGFPPLHHPLPEEKLIIETDASDDYWGGMLKAIKINEGTNTELICRYASGSFKAAERNYHSNDKETLAVINTIKKFSIYLTPVHFLIRTD 621

HSP 2 Score: 36.965 bits (84), Expect = 4.370e-14
Identity = 18/31 (58.06%), Postives = 21/31 (67.74%), Query Frame = -2 
Query:  105 R*QAILSVFDFDIEFIKGDKNSIPDFLTREF 197
            R QA LS + FD+E IKG  N   DFL+REF
Sbjct:  644 RWQAWLSHYSFDVEHIKGTDNHFADFLSREF 674
[Close]
BLAST of FC919330 vs. ExPASy Swiss-Prot
Match: POL_CAMVN (Enzymatic polyprotein OS=Cauliflower mosaic virus (strain NY8153) GN=ORF V PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 9.578e-14
Identity = 37/109 (33.94%), Postives = 58/109 (53.21%), Query Frame = -3 
Query:  263 PAAWTDEHTKTVRLIKNSVKCIPCLYLANPTLPKIVETDASDLGYGGILK----QKENDKEQIIQYVSAHWNDCQKNYSTIKKEILSIVLCISKFQSDLLNQKFLLRID 577
            P  WT E T  ++ +K +++  P L+   P    I+ETDASD  +GG+LK     +  + E I +Y S  +   ++NY +  KE L+++  I KF   L    FL+R D
Sbjct:  514 PWKWTKEDTLYMQKVKKNLQGFPPLHHPLPEEKLIIETDASDDYWGGMLKAIKINEGTNTELICRYRSGSFKAAERNYHSNDKETLAVINTIKKFSIYLTPVHFLIRTD 622

HSP 2 Score: 36.965 bits (84), Expect = 9.578e-14
Identity = 18/31 (58.06%), Postives = 21/31 (67.74%), Query Frame = -2 
Query:  105 R*QAILSVFDFDIEFIKGDKNSIPDFLTREF 197
            R QA LS + FD+E IKG  N   DFL+REF
Sbjct:  645 RWQAWLSHYSFDVEHIKGTDNHFADFLSREF 675
[Close]
BLAST of FC919330 vs. ExPASy Swiss-Prot
Match: POL_CYLCV (Enzymatic polyprotein OS=Cestrum yellow leaf curling virus GN=ORF V PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 6.565e-11
Identity = 35/114 (30.70%), Postives = 58/114 (50.88%), Query Frame = -3 
Query:  263 PAAWTDEHTKTVRLIKNSVKCIPCLYLANPTLPKIVETDASDLGYGGILK---------QKENDKEQIIQYVSAHWNDCQKNYSTIKKEILSIVLCISKFQSDLLNQKFLLRID 577
            P  W+D  +K V  IK   K +  LY   P    I+ETDAS   + G LK          K    E++ +Y S  ++  ++ Y+  +KE L+ +  + K++++LL ++F LR D
Sbjct:  474 PWHWSDLDSKLVNQIKKKCKDLSPLYFPKPEDYLIIETDASGDTWAGCLKAAELLFPKGTKNKVVERLCKYTSGIFSSAEQKYTVHEKETLAALKTMRKWKAELLPKEFTLRTD 587

HSP 2 Score: 31.9574 bits (71), Expect = 6.565e-11
Identity = 13/21 (61.90%), Postives = 18/21 (85.71%), Query Frame = -2 
Query:   99 IEFIKGDKNSIPDFLTREFLQ 161
            +E+IKG+KNS+ D LTRE+ Q
Sbjct:  622 VEYIKGEKNSLADTLTREWKQ 642
The following BLAST results are available for this feature:
BLAST of FC919330 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 8
Match NameE-valueIdentityDescription
POL_CERV2.849e-1736.11Enzymatic polyprotein OS=Carnation etched ring vir... [more]
POL_FMVD1.535e-1438.10Enzymatic polyprotein OS=Figwort mosaic virus (str... [more]
POL_CAMVS1.993e-1434.86Enzymatic polyprotein OS=Cauliflower mosaic virus ... [more]
POL_CAMVD1.993e-1434.86Enzymatic polyprotein OS=Cauliflower mosaic virus ... [more]
POL_CAMVE4.370e-1433.94Enzymatic polyprotein OS=Cauliflower mosaic virus ... [more]
POL_CAMVC4.370e-1433.94Enzymatic polyprotein OS=Cauliflower mosaic virus ... [more]
POL_CAMVN9.578e-1433.94Enzymatic polyprotein OS=Cauliflower mosaic virus ... [more]
POL_CYLCV6.565e-1130.70Enzymatic polyprotein OS=Cestrum yellow leaf curli... [more]
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Properties
Property NameValue
Genbank descriptionC16000A03SK PCR Citrus clementina cDNA clone C16000A03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC919330 ID=FC919330; Name=FC919330; organism=Citrus clementina; type=EST; length=588bp
TGAAGGAGTTGATTGGGATTCTTTGGAGGGTTTTTGGGAATCAGTGTCTT
TGGCTATGCCTTTTCCTTTGTCTCGACGCTTTGGCGGCATTATCTGTTTT
GAAGAAATTCACGAGTTAGAAAATCAGGAATAGAATTTTTATCGCCTTTA
ATAAATTCAATATCAAAATCAAAAACACTTAAAATGGCTTGTCATCGTGC
AAAAATTTGTTTTGATGCAATGTTTTGAACATCTTTTTCTAAACATGTTT
TGCAGCTTTGCAATCTATTCTAAGTAAAAATTTTTGATTTAGTAAATCAC
TTTGGAATTTTGATATGCACAAAACAATGGAAAGAATTTCTTTTTTAATA
GTAGAATAGTTCTTTTGACAATCATTCCAATGTGCAGAAACATATTGAAT
AATTTGTTCTTTATCATTTTCTTTTTGCTTTAAAATACCTCCATAACCTA
AATCAGATGCATCAGTTTCAACTATCTTTGGCAATGTAGGATTTGCAAGA
TATAAACATGGAATGCACTTAACAGAATTCTTTATTAATCTAACAGTCTT
TGTATGCTCATCAGTCCATGCAGCAGGTTTCTTTTTTA
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