FC920720

Overview
NameFC920720
Unique NameFC920720
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length709
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig14122 contig Ccv1_Contig14122:50..758. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 362.844 bits (930), Expect = 9.969e-100
Identity = 172/208 (82.69%), Postives = 191/208 (91.83%), Query Frame = 2 
Query:   86 WPKKDKRRFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            WPKKDKRRFLH VYRVGDLDRTI+FYTECFGMK+LRKRDVPEEKYSNAFLGFGPE S+FVVELTYNYGV+SYDIGTGFGHFAI+T+DV K+VE +RAKGGNVTREPGPVKGG + IAFVKDPDGY FELIQRGPTPEPLCQVMLR GDL R++KF EKALGM+LLR +++PEY  T+ M+GYAEE ++ VLELTYNYGVTEYT GNAY
Sbjct:   10 WPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAY 216

HSP 2 Score: 98.5969 bits (244), Expect = 3.507e-20
Identity = 52/115 (45.22%), Postives = 68/115 (59.13%), Query Frame = 2 
Query:  128 RVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIR----AKGGNVTREPGPVKGGTTHIAFVKDPDGY 460
            RVGDLDR +KF  +  GM+LLR+ + PE   +   +G+  E    V+ELTYNYGVT Y  G  +   AI T+DVYK  E ++      GG +TRE GP+ G  T I    DPDG+
Sbjct:  154 RVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGW 267
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 358.992 bits (920), Expect = 1.440e-98
Identity = 171/209 (81.82%), Postives = 187/209 (89.47%), Query Frame = 2 
Query:   86 WPKKDKRRFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGG-NVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            WPKKDK+R LHAVYRVGDLDRTIK YTECFGMKLLRKRDVPEEKY+NAFLGFGPE ++F +ELTYNYGV  YDIG GFGHFAIATEDVYKL E I++     +TREPGPVKGG+T IAF +DPDGY+FELIQRGPTPEPLCQVMLR GDL RSIKFYEKALGMKLLR  D P+YKYT+AMLGYA+ED+TTV+ELTYNYGVTEYT GNAY
Sbjct:   17 WPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAY 225

HSP 2 Score: 124.79 bits (312), Expect = 4.569e-28
Identity = 63/120 (52.50%), Postives = 78/120 (65.00%), Query Frame = 2 
Query:  128 RVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENI----RAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELI 475
            RVGDLDR+IKFY +  GMKLLRK+DVP+ KY+ A LG+  E    V+ELTYNYGVT Y  G  +   AI TEDVYK  E +    +  GG + R+PGP+ G  T IA   DPDG+   L+
Sbjct:  162 RVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLV 281
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 167.933 bits (424), Expect = 4.707e-41
Identity = 78/124 (62.90%), Postives = 95/124 (76.61%), Query Frame = 2 
Query:  107 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 478
            R LH + RVGDLD++IKFYTE  GM+LLR  +  E +Y+ AF+G+G E    V+ELTYN+G T YD+GT FGH AI  +D+Y   + I+A GGNVTRE GPVKGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 86.2705 bits (212), Expect = 1.801e-16
Identity = 39/70 (55.71%), Postives = 51/70 (72.86%), Query Frame = 2 
Query:  500 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            +   MLR GDL +SIKFY + +GM+LLRT +  EY+YTLA +GY +E Q  V+ELTYN+G TEY  G A+
Sbjct:    6 ILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLGTAF 75
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_VIBCH (Probable lactoylglutathione lyase OS=Vibrio cholerae GN=gloA PE=3 SV=2)

HSP 1 Score: 164.081 bits (414), Expect = 6.796e-40
Identity = 76/124 (61.29%), Postives = 93/124 (75.00%), Query Frame = 2 
Query:  107 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 478
            R LH + RVGDLD++I+FYT+  GM LLRK +  E KY+ AFLG+G E    V+ELTYN+GV  Y+ G  +GH AI  +D+Y   + I+A GG VTREPGPVKGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 87.8113 bits (216), Expect = 6.190e-17
Identity = 40/70 (57.14%), Postives = 50/70 (71.43%), Query Frame = 2 
Query:  500 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            +   MLR GDL +SI+FY + +GM LLR  +  EYKYTLA LGY +E Q  V+ELTYN+GV +Y  GNAY
Sbjct:    6 ILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAY 75
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.361e-38
Identity = 74/124 (59.68%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:  107 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 478
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 90.5077 bits (223), Expect = 9.550e-18
Identity = 43/70 (61.43%), Postives = 50/70 (71.43%), Query Frame = 2 
Query:  500 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            L   MLR GDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y  G AY
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAY 72
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.361e-38
Identity = 74/124 (59.68%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:  107 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 478
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 90.5077 bits (223), Expect = 9.550e-18
Identity = 43/70 (61.43%), Postives = 50/70 (71.43%), Query Frame = 2 
Query:  500 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            L   MLR GDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y  G AY
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAY 72
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.361e-38
Identity = 74/124 (59.68%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:  107 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 478
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 90.5077 bits (223), Expect = 9.550e-18
Identity = 43/70 (61.43%), Postives = 50/70 (71.43%), Query Frame = 2 
Query:  500 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            L   MLR GDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y  G AY
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAY 72
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.417e-37
Identity = 74/124 (59.68%), Postives = 91/124 (73.39%), Query Frame = 2 
Query:  107 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 478
            R LH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GPVKGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 90.5077 bits (223), Expect = 9.550e-18
Identity = 43/70 (61.43%), Postives = 49/70 (70.00%), Query Frame = 2 
Query:  500 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            L   MLR GDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV  Y  GNAY
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAY 72
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_SALTI (Lactoylglutathione lyase OS=Salmonella typhi GN=gloA PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.417e-37
Identity = 74/124 (59.68%), Postives = 91/124 (73.39%), Query Frame = 2 
Query:  107 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 478
            R LH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GPVKGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 90.5077 bits (223), Expect = 9.550e-18
Identity = 43/70 (61.43%), Postives = 49/70 (70.00%), Query Frame = 2 
Query:  500 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            L   MLR GDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV  Y  GNAY
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAY 72
[Close]
BLAST of FC920720 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 1.567e-36
Identity = 68/122 (55.74%), Postives = 90/122 (73.77%), Query Frame = 2 
Query:  113 LHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 478
            LH + RVGDLD++++FY +  GM LLRK+D P  +++ AF+G+G E  + V+ELT+N+G   YD+G GFGH A+  ED+Y   + IR KGG V REPGP+K GTT IAFV+DPDGY  ELIQ
Sbjct:    4 LHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLGNGFGHIALGVEDIYSTCDKIRDKGGKVVREPGPMKHGTTVIAFVEDPDGYKIELIQ 125

HSP 2 Score: 71.633 bits (174), Expect = 4.591e-12
Identity = 32/70 (45.71%), Postives = 46/70 (65.71%), Query Frame = 2 
Query:  500 LCQVMLRAGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTXGNAY 709
            L   M+R GDL +S++FY   LGM LLR  D P  ++TLA +GY +E +  V+ELT+N+G  +Y  GN +
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLGNGF 72
The following BLAST results are available for this feature:
BLAST of FC920720 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28
Match NameE-valueIdentityDescription
LGUL_BRAOG9.969e-10082.69Putative lactoylglutathione lyase OS=Brassica oler... [more]
LGUL_ORYSJ1.440e-9881.82Lactoylglutathione lyase OS=Oryza sativa subsp. ja... [more]
LGUL_VIBPA4.707e-4162.90Probable lactoylglutathione lyase OS=Vibrio paraha... [more]
LGUL_VIBCH6.796e-4061.29Probable lactoylglutathione lyase OS=Vibrio choler... [more]
LGUL_SHIFL6.361e-3859.68Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI6.361e-3859.68Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO576.361e-3859.68Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_SALTY1.417e-3759.68Lactoylglutathione lyase OS=Salmonella typhimurium... [more]
LGUL_SALTI1.417e-3759.68Lactoylglutathione lyase OS=Salmonella typhi GN=gl... [more]
LGUL_SYNY31.567e-3655.74Probable lactoylglutathione lyase OS=Synechocystis... [more]

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Properties
Property NameValue
Genbank descriptionC32012B10EF RVDevelop1 Citrus clementina cDNA clone C32012B10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920720 ID=FC920720; Name=FC920720; organism=Citrus clementina; type=EST; length=709bp
TGCGACTTCACTCCGACTTTCTGAGTGATTCCATATCAAAATGGCTGAGG
CTTCTCCTGCTGCTGCTAATGCCGAGTTGCTGGAATGGCCAAAGAAAGAT
AAGCGACGCTTCCTTCATGCTGTGTATCGTGTTGGTGATCTTGATCGCAC
CATCAAATTTTACACTGAATGCTTTGGGATGAAGCTGTTGAGAAAGAGAG
ACGTCCCAGAAGAGAAATACTCAAATGCCTTTCTTGGATTTGGCCCCGAA
CAGTCTCACTTTGTTGTGGAGTTAACATACAACTATGGTGTAACTTCATA
TGATATTGGAACTGGTTTTGGCCATTTTGCAATTGCAACTGAAGATGTTT
ACAAACTGGTTGAAAACATTCGTGCCAAGGGTGGCAATGTCACTAGAGAG
CCTGGTCCAGTTAAAGGTGGAACAACTCACATTGCCTTTGTGAAGGATCC
CGATGGTTATATTTTTGAACTCATCCAGAGAGGTCCAACTCCTGAACCAC
TCTGTCAAGTAATGCTTCGCGCTGGCGATCTTGGTCGCTCCATCAAGTTC
TATGAAAAGGCTTTGGGGATGAAACTGTTAAGGACAGTTGATAAACCTGA
ATACAAGTACACCCTGGCCATGCTTGGATATGCTGAGGAAGATCAGACTA
CTGTTCTGGAGTTAACTTATAATTATGGTGTGACTGAATATACCNAGGGA
AATGCATAT
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