FC930219

Overview
NameFC930219
Unique NameFC930219
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length702
Libraries
Library NameType
PostHarvC1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC930219_ssr121 microsatellite FC930219_ssr121:121..132. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig13179 contig Ccv1_Contig13179:44..747. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP22_ORYSJ (Probable aquaporin PIP2-2 OS=Oryza sativa subsp. japonica GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 242.662 bits (618), Expect = 2.049e-78
Identity = 122/173 (70.52%), Postives = 133/173 (76.88%), Query Frame = 1 
Query:   88 MGKDVEVAXXXXXXXXFSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLNTDQ--CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYTR 600
            M KD+E +        FSAKDY DPPPAPLID EEL +WS YRAVIAEFIA            IGYK Q+D  +NT    C GVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL RKVSLIRA+LY++AQCLGAICG G VK FQ SYY RYGGGANEL+DGY++
Sbjct:    1 MAKDIEASAPEGGE--FSAKDYTDPPPAPLIDVEELTKWSLYRAVIAEFIATLLFLYITVATVIGYKHQSDATVNTTDAACSGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYIIAQCLGAICGVGLVKGFQSSYYARYGGGANELSDGYSK 171

HSP 2 Score: 71.633 bits (174), Expect = 2.049e-78
Identity = 33/35 (94.29%), Postives = 35/35 (100.00%), Query Frame = 3 
Query:  597 KGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            KGTGLGAEIIGTFVLVYTVFSATDPKR+ARDSH+P
Sbjct:  171 KGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHIP 205
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP24_ARATH (Probable aquaporin PIP2-4 OS=Arabidopsis thaliana GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 1.316e-77
Identity = 116/171 (67.84%), Postives = 128/171 (74.85%), Query Frame = 1 
Query:   88 MGKDVEVAXXXXXXXXFSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLNTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYTR 600
            M KD++V          +A+DY DPPPAP  D EEL +W  YRAVIAEF+A            IGYK+QTD       CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVT GLFL RKVSL+R +LY+VAQCLGAICGCGFVKAFQ SYY RYGGGANELADGY +
Sbjct:    1 MAKDLDVNESGPP----AARDYKDPPPAPFFDMEELRKWPLYRAVIAEFVATLLFLYVSILTVIGYKAQTDATAGGVDCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTVGLFLARKVSLVRTVLYIVAQCLGAICGCGFVKAFQSSYYTRYGGGANELADGYNK 167

HSP 2 Score: 72.4034 bits (176), Expect = 1.316e-77
Identity = 34/36 (94.44%), Postives = 36/36 (100.00%), Query Frame = 3 
Query:  594 HKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            +KGTGLGAEIIGTFVLVYTVFSATDPKR+ARDSHVP
Sbjct:  166 NKGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVP 201
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP24_MAIZE (Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 2.239e-77
Identity = 121/173 (69.94%), Postives = 133/173 (76.88%), Query Frame = 1 
Query:   88 MGKDVEVAXXXXXXXXFSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLNTDQ--CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYTR 600
            M KD+E +        FSAKDY DPPPAPLID EEL +WS YRAVIAEFIA            IGYK QTD + +     CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL RKVSL+RALLY++AQCLGAICG G VK FQ +YY RYGGGANEL+DGY++
Sbjct:    1 MAKDIEASGPEAGE--FSAKDYTDPPPAPLIDAEELTQWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIIAQCLGAICGVGLVKGFQSAYYVRYGGGANELSDGYSK 171

HSP 2 Score: 70.8626 bits (172), Expect = 2.239e-77
Identity = 34/35 (97.14%), Postives = 34/35 (97.14%), Query Frame = 3 
Query:  597 KGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            KGTGL AEIIGTFVLVYTVFSATDPKRSARDSHVP
Sbjct:  171 KGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVP 205
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP25_ARATH (Probable aquaporin PIP2-5 OS=Arabidopsis thaliana GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 241.891 bits (616), Expect = 4.967e-77
Identity = 114/154 (74.03%), Postives = 125/154 (81.17%), Query Frame = 1 
Query:  136 FSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLNTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYT 597
            FS KDY DPPP PL D  ELG+WSFYRA+IAEFIA            IGYKSQTDP LN DQC GVG+LGIAWAFGGMIFILVYCTAGISGGHINPAVTFGL L RKV+L+RA++YMVAQCLGAICG   VKAFQ +Y+ RYGGGAN L+DGY+
Sbjct:   12 FSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDPALNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVTLVRAVMYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYS 165

HSP 2 Score: 67.781 bits (164), Expect = 4.967e-77
Identity = 32/34 (94.12%), Postives = 33/34 (97.06%), Query Frame = 3 
Query:  600 GTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            GTG+ AEIIGTFVLVYTVFSATDPKRSARDSHVP
Sbjct:  167 GTGVAAEIIGTFVLVYTVFSATDPKRSARDSHVP 200
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP21_MAIZE (Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 238.039 bits (606), Expect = 8.450e-77
Identity = 120/173 (69.36%), Postives = 132/173 (76.30%), Query Frame = 1 
Query:   88 MGKDVEVAXXXXXXXXFSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLNTDQ--CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYTR 600
            MGKD +V         F+AKDY DPPPAPLID  ELG WS YRAVIAEFIA            IGYK QTD + +     CGGVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFL RKVSL+RALLY+VAQCLGAICG G VKAFQ +Y++RYGGGAN LA GY+R
Sbjct:    1 MGKD-DVIESGAGGGEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASASGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIVAQCLGAICGVGLVKAFQSAYFDRYGGGANSLASGYSR 172

HSP 2 Score: 70.8626 bits (172), Expect = 8.450e-77
Identity = 33/35 (94.29%), Postives = 35/35 (100.00%), Query Frame = 3 
Query:  597 KGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            +GTGLGAEIIGTFVLVYTVFSATDPKR+ARDSHVP
Sbjct:  172 RGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVP 206
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP21_ORYSJ (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 1.437e-76
Identity = 121/173 (69.94%), Postives = 132/173 (76.30%), Query Frame = 1 
Query:   88 MGKDVEVAXXXXXXXXFSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLNTDQ--CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYTR 600
            MGKD EV         F+AKDY DPPPAPLID  ELG WS YRAVIAEFIA            IGYK QTD + +     CGGVG+LGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL RKVSL+RA+LY+VAQCLGAICG G VKAFQ +Y+NRYGGGAN LA GY++
Sbjct:    1 MGKD-EVMESGGAAGEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASASGADAACGGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILYIVAQCLGAICGVGLVKAFQSAYFNRYGGGANTLAAGYSK 172

HSP 2 Score: 69.707 bits (169), Expect = 1.437e-76
Identity = 33/35 (94.29%), Postives = 34/35 (97.14%), Query Frame = 3 
Query:  597 KGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            KGTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVP
Sbjct:  172 KGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVP 206
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP21_ARATH (Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 1.875e-76
Identity = 119/170 (70.00%), Postives = 127/170 (74.71%), Query Frame = 1 
Query:   88 MGKDVEVAXXXXXXXXFSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLNTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYT 597
            M KDVE          F  +DY DPPPAP ID  EL +WSFYRAVIAEF+A            IGYK Q+D +     CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL RKVSL RALLY++AQCLGAICG GFVKAFQ SYY RYGGGAN LADGY+
Sbjct:    1 MAKDVEAVPGEG----FQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYKIQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYS 166

HSP 2 Score: 68.9366 bits (167), Expect = 1.875e-76
Identity = 33/34 (97.06%), Postives = 33/34 (97.06%), Query Frame = 3 
Query:  600 GTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            GTGL AEIIGTFVLVYTVFSATDPKRSARDSHVP
Sbjct:  168 GTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVP 201
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP27_MAIZE (Aquaporin PIP2-7 OS=Zea mays GN=PIP2-7 PE=2 SV=1)

HSP 1 Score: 237.269 bits (604), Expect = 2.445e-76
Identity = 117/172 (68.02%), Postives = 129/172 (75.00%), Query Frame = 1 
Query:   88 MGKDVEVAXXXXXXXXFSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNL-NTDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYTR 600
            M KDVE          +SAKDYHDPPPAPLID +EL +WS YRA IAEFIA            IGYK Q+D  +    +C GVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSL+RALLYM+AQC GAICG G  K FQKS+YNRYGGG N ++DGY +
Sbjct:    1 MAKDVEQVTEQGE---YSAKDYHDPPPAPLIDPDELTKWSLYRAAIAEFIATLLFLYITVLTIIGYKRQSDTKIPGNTECDGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLVRALLYMIAQCAGAICGAGLAKGFQKSFYNRYGGGVNTVSDGYNK 169

HSP 2 Score: 70.0922 bits (170), Expect = 2.445e-76
Identity = 33/36 (91.67%), Postives = 35/36 (97.22%), Query Frame = 3 
Query:  594 HKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            +KGT LGAEIIGTFVLVYTVFSATDPKR+ARDSHVP
Sbjct:  168 NKGTALGAEIIGTFVLVYTVFSATDPKRNARDSHVP 203
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP22_MAIZE (Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 3.189e-76
Identity = 119/175 (68.00%), Postives = 132/175 (75.43%), Query Frame = 1 
Query:   88 MGKDVEVAXXXXXXXXFSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLN----TDQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYTR 600
            MGKD +V         F+AKDY DPPPAPL+D  ELG WS YRAVIAEFIA            IGYK QTD + +       CGGVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFL RKVSL+RALLYMVAQCLGA+CG G VKAFQ +Y++RYGGGAN LA GY+R
Sbjct:    1 MGKD-DVVQSGAGGGEFAAKDYTDPPPAPLVDAAELGSWSLYRAVIAEFIATLLFLYVTVATVIGYKHQTDASASGAGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYMVAQCLGAVCGVGLVKAFQSAYFDRYGGGANSLASGYSR 174

HSP 2 Score: 68.5514 bits (166), Expect = 3.189e-76
Identity = 31/35 (88.57%), Postives = 34/35 (97.14%), Query Frame = 3 
Query:  597 KGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            +G GLGAEI+GTFVLVYTVFSATDPKR+ARDSHVP
Sbjct:  174 RGAGLGAEIVGTFVLVYTVFSATDPKRNARDSHVP 208
[Close]
BLAST of FC930219 vs. ExPASy Swiss-Prot
Match: PIP23_MAIZE (Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 4.159e-76
Identity = 116/157 (73.89%), Postives = 126/157 (80.25%), Query Frame = 1 
Query:  136 FSAKDYHDPPPAPLIDFEELGRWSFYRAVIAEFIAXXXXXXXXXXXXIGYKSQTDPNLNTDQ--CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLIRALLYMVAQCLGAICGCGFVKAFQKSYYNRYGGGANELADGYTR 600
            FSAKDY DPPPAPLID +EL +WS YRAVIAEFIA            IGYK QTD   +     CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL RKVSL+RALLY++AQCLGAICG G VK FQ +YY RYGGGANEL+DGY++
Sbjct:   16 FSAKDYTDPPPAPLIDADELTKWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDAAASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIIAQCLGAICGVGLVKGFQSAYYVRYGGGANELSDGYSK 172

HSP 2 Score: 70.8626 bits (172), Expect = 4.159e-76
Identity = 34/35 (97.14%), Postives = 34/35 (97.14%), Query Frame = 3 
Query:  597 KGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVP 701
            KGTGL AEIIGTFVLVYTVFSATDPKRSARDSHVP
Sbjct:  172 KGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVP 206
The following BLAST results are available for this feature:
BLAST of FC930219 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 89
Match NameE-valueIdentityDescription
PIP22_ORYSJ2.049e-7870.52Probable aquaporin PIP2-2 OS=Oryza sativa subsp. j... [more]
PIP24_ARATH1.316e-7767.84Probable aquaporin PIP2-4 OS=Arabidopsis thaliana ... [more]
PIP24_MAIZE2.239e-7769.94Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1[more]
PIP25_ARATH4.967e-7774.03Probable aquaporin PIP2-5 OS=Arabidopsis thaliana ... [more]
PIP21_MAIZE8.450e-7769.36Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2[more]
PIP21_ORYSJ1.437e-7669.94Probable aquaporin PIP2-1 OS=Oryza sativa subsp. j... [more]
PIP21_ARATH1.875e-7670.00Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1... [more]
PIP27_MAIZE2.445e-7668.02Aquaporin PIP2-7 OS=Zea mays GN=PIP2-7 PE=2 SV=1[more]
PIP22_MAIZE3.189e-7668.00Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1[more]
PIP23_MAIZE4.159e-7673.89Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionC34005A09EF PostHarvC1 Citrus clementina cDNA clone C34005A09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930219 ID=FC930219; Name=FC930219; organism=Citrus clementina; type=EST; length=702bp
CATCATTAAACTCTGTAGCAACACAACAGTCGTAGCTCTTGTTTCTCCCT
CTCGTTTAATTAACAAAACAAAAATAAAAGGGAAAAAATGGGGAAGGATG
TTGAAGTTGCTGAGCAGCAAGGCGGCGGCGGCGAGTTCTCAGCCAAGGAC
TACCACGACCCCCCTCCGGCGCCGCTGATCGATTTCGAGGAGCTGGGCAG
GTGGTCTTTTTACAGAGCTGTAATTGCTGAGTTCATCGCTACGCTTTTGT
TTCTTTATGTTACCGTCTTGACTGTGATTGGATACAAGAGCCAGACTGAT
CCCAACTTGAATACCGATCAGTGTGGTGGCGTTGGCATTCTTGGCATCGC
TTGGGCCTTTGGTGGCATGATCTTCATTCTCGTTTACTGCACAGCCGGTA
TTTCTGGTGGACATATCAACCCGGCTGTGACATTCGGACTGTTCTTGGGG
CGCAAGGTGTCGCTCATCCGAGCTCTTTTGTACATGGTAGCACAGTGCTT
GGGTGCAATCTGTGGTTGTGGGTTTGTGAAGGCTTTCCAAAAATCATACT
ACAACAGGTATGGAGGTGGGGCCAATGAGCTAGCTGATGGATACACAAGG
GCACCGGACTGGGTGCTGAGATCATCGGTACTTTCGTTCTTGTCTACACT
GTTTTCTCTGCCACTGATCCTAAGAGGAGCGCAAGAGACTCCCATGTTCC
GG
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