FC930308

Overview
NameFC930308
Unique NameFC930308
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length741
Libraries
Library NameType
PostHarvC1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC930308_ssr21 microsatellite FC930308_ssr21:21..32. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig15134 contig Ccv1_Contig15134:1..741. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_AEDAE (Mediator of RNA polymerase II transcription subunit 10 OS=Aedes aegypti GN=MED10 PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 9.231e-19
Identity = 45/129 (34.88%), Postives = 78/129 (60.47%), Query Frame = 2 
Query:  164 PKQNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCNIQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 550
            P +NL    N ++  +  + Q+ + VS F    Q  L Q++ SLV+ L  + KL  + ++ VP+EV + ID G+NP  +T+D I+  + KN+  KGK D+++  + +L+ EL++TFP E+  Y+ IR +
Sbjct:    4 PLENLE---NHLEMFIENVRQIRIIVSDFQPQGQNVLNQKIQSLVTGLQEIDKLKNQVDVNVPLEVFDYIDQGRNPQLYTKDCIDKALTKNEEVKGKIDSYRKFKSNLMKELDETFPTEIAKYKAIRGD 129
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_ANOGA (Mediator of RNA polymerase II transcription subunit 10 OS=Anopheles gambiae GN=MED10 PE=3 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 2.056e-18
Identity = 45/129 (34.88%), Postives = 77/129 (59.69%), Query Frame = 2 
Query:  164 PKQNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCNIQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 550
            P +NL    N ++  +  + Q+ + VS F    Q  L Q++ SLV+ L  + KL  + ++ VP+EV + ID G+NP  +T+D I+  + KN+  KGK D+++  + +L+ EL +TFP E+  Y+ IR +
Sbjct:    4 PLENLE---NHLEMFIENVRQIRIIVSDFQPQGQNVLNQKIQSLVTGLQEIDKLKNQIDVNVPLEVFDYIDQGRNPQLYTKDCIDKALTKNEEVKGKIDSYRKFKSNLMKELSETFPVEISKYKAIRGD 129
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_MOUSE (Mediator of RNA polymerase II transcription subunit 10 OS=Mus musculus GN=Med10 PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.288e-16
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 2 
Query:  197 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 544
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLSQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_PONAB (Mediator of RNA polymerase II transcription subunit 10 OS=Pongo abelii GN=MED10 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.600e-16
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 2 
Query:  197 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 544
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_HUMAN (Mediator of RNA polymerase II transcription subunit 10 OS=Homo sapiens GN=MED10 PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.600e-16
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 2 
Query:  197 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 544
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_BOVIN (Mediator of RNA polymerase II transcription subunit 10 OS=Bos taurus GN=MED10 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.600e-16
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 2 
Query:  197 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 544
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_XENTR (Mediator of RNA polymerase II transcription subunit 10 OS=Xenopus tropicalis GN=med10 PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 7.314e-16
Identity = 43/117 (36.75%), Postives = 72/117 (61.54%), Query Frame = 2 
Query:  197 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 544
            ++K +  + QL + VS F  +SQ  L Q+LN LV+ L ++ K  ++ + I VP+EV + ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  Y+ IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFLVTGLQDIDKCRQQLHDITVPLEVFDYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLKKFKSLLIQELSKVFPEDMAKYKAIR 128
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_XENLA (Mediator of RNA polymerase II transcription subunit 10 OS=Xenopus laevis GN=med10 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.248e-15
Identity = 42/126 (33.33%), Postives = 75/126 (59.52%), Query Frame = 2 
Query:  170 QNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 544
            +  + + + ++K +  + QL + VS F  +SQ  L Q+LN LV  L ++ K  ++ + I VP+EV + ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  Y+ +R
Sbjct:    3 EKFDNLEDHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFLVRGLQDIDKCRQQLHDITVPLEVFDYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLKKFKSLLIQELSKVFPEDMAKYKAVR 128
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_MACFA (Mediator of RNA polymerase II transcription subunit 10 OS=Macaca fascicularis GN=MED10 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.630e-15
Identity = 42/117 (35.90%), Postives = 70/117 (59.83%), Query Frame = 2 
Query:  197 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 544
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+  L + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQGLSKVFPEDMAKYRSIR 128
[Close]
BLAST of FC930308 vs. ExPASy Swiss-Prot
Match: MED10_DANRE (Mediator of RNA polymerase II transcription subunit 10 OS=Danio rerio GN=med10 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.379e-14
Identity = 40/119 (33.61%), Postives = 70/119 (58.82%), Query Frame = 2 
Query:  197 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 550
            ++K +  + QL + VS F  +SQ  L Q+LN +++ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D     +  L+ EL + FP+E+  Y+ I  +
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQTGLNQKLNFMITGLQDIEKCRQQLHDINVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLTKFKSLLISELGKVFPEEMAKYKAIHGD 130
The following BLAST results are available for this feature:
BLAST of FC930308 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
MED10_AEDAE9.231e-1934.88Mediator of RNA polymerase II transcription subuni... [more]
MED10_ANOGA2.056e-1834.88Mediator of RNA polymerase II transcription subuni... [more]
MED10_MOUSE4.288e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_PONAB5.600e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_HUMAN5.600e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_BOVIN5.600e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_XENTR7.314e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_XENLA1.248e-1533.33Mediator of RNA polymerase II transcription subuni... [more]
MED10_MACFA3.630e-1535.90Mediator of RNA polymerase II transcription subuni... [more]
MED10_DANRE1.379e-1433.61Mediator of RNA polymerase II transcription subuni... [more]

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Properties
Property NameValue
Genbank descriptionC34006A11EF PostHarvC1 Citrus clementina cDNA clone C34006A11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930308 ID=FC930308; Name=FC930308; organism=Citrus clementina; type=EST; length=741bp
GGCAGAAGTTTTGGGGTCCTGAAGAAGAAGAAAACTGTAATTACAGTTTC
CTGATACGAGAGAAAGAAAGATGGATGGACCGGTGGGGGGCAGCAGAGCA
AGTGGTGGCAATGGAATGGTGAGCAATCAAGCAAACGATACAACAACTGT
GGCAGCTGACGATCCGAAGCAGAACTTGAACCAGGTTATTAATTCAGTTC
AGAAGACTCTTGGGCTCCTACATCAACTCTACCTTACCGTATCTTCCTTC
AATGCTGCCTCCCAGCTCCCTCTCCTACAAAGACTCAATTCTCTTGTTTC
GGAGCTTGACAACATGGTTAAGTTGTCTGAGAAGTGCAATATTCAAGTGC
CCATGGAAGTGCTTAATTTGATTGATGACGGAAAAAATCCTGATGAATTC
ACAAGGGATGTTATAAACAGCTGCATTGCTAAAAACCAAGTCACCAAAGG
AAAAACTGATGCTTTCAAGAGTTTACGTAAGCATCTTTTGGACGAACTTG
AGCAGACATTTCCGGATGAAGTTGAAGCATATAGAGAGATACGTGCAAAT
TCTGCTGCTGAAGCAAAGCGTCTTGCTCAATCACAAAGTATGTTGCCAAA
TGGAGATGTGAAAGTTAAATCTGAGCTTTAAATGTCCCATTTCATGCAGA
GTGTTGGGGCTCTTTCGACTACTACTTTCTCAATGTAATGTCATGAGTCG
TTCCAGTTCAGCAGCATTCAATGATTAAANTCTGAAAAATT
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