FC931559

Overview
NameFC931559
Unique NameFC931559
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length683
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC931559_ssr95 microsatellite FC931559_ssr95:95..106. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig584 contig Ccv1_Contig584:11..694. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS1_ARATH (Ribose-phosphate pyrophosphokinase 1 OS=Arabidopsis thaliana GN=PRS1 PE=2 SV=2)

HSP 1 Score: 179.489 bits (454), Expect = 3.086e-47
Identity = 86/107 (80.37%), Postives = 98/107 (91.59%), Query Frame = 2 
Query:  320 AAVRCDMAEALNFPNGKPNIPVLNERTLPKFLETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            A V+CDM E+LN  NG P+IP++NERTLPKFLE+ARMEK+VNRT N R+KLFSGTANPAL+QEIA YMG++LGK+NIKRFADGEIYVQLQESVRGCDVYLVQPTC P
Sbjct:   46 ACVKCDMPESLNVGNGNPSIPIINERTLPKFLESARMEKSVNRT-NTRLKLFSGTANPALAQEIAWYMGLDLGKVNIKRFADGEIYVQLQESVRGCDVYLVQPTCTP 151

HSP 2 Score: 30.4166 bits (67), Expect = 3.086e-47
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDAC 683
            C+P    +ENLMELLIM+DAC
Sbjct:  149 CTPT---NENLMELLIMVDAC 166
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS2_SPIOL (Ribose-phosphate pyrophosphokinase 2, chloroplastic OS=Spinacia oleracea GN=PRS2 PE=2 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 1.560e-39
Identity = 69/107 (64.49%), Postives = 92/107 (85.98%), Query Frame = 2 
Query:  323 AVRCDMAEALNFPNGKPNIPVLNERTLPKFLETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPPA 643
            +V+C+  E L   NGKP++P+L+E++LPKFL + R+E +VNR+ N R+KLFSG ANPAL++E+A YMG+ELGK+ IKRFADGEIYVQL+ESVRGCDV+++QPT PPA
Sbjct:   39 SVKCNTVEPLKLVNGKPSVPILDEQSLPKFLHSKRLESSVNRS-NTRLKLFSGLANPALAKEVAWYMGLELGKVKIKRFADGEIYVQLEESVRGCDVFIIQPTSPPA 144

HSP 2 Score: 31.9574 bits (71), Expect = 1.560e-39
Identity = 16/20 (80.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDAC 683
            SPP   +ENLMELLIMIDAC
Sbjct:  141 SPPA--NENLMELLIMIDAC 158
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS2_ARATH (Ribose-phosphate pyrophosphokinase 2 OS=Arabidopsis thaliana GN=PRS2 PE=1 SV=2)

HSP 1 Score: 149.828 bits (377), Expect = 2.197e-38
Identity = 70/111 (63.06%), Postives = 93/111 (83.78%), Query Frame = 2 
Query:  308 VCNAAAVRCDMAEALNFPNGKPNIPVLNERTLPKFLETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            +C +   +C++ +ALN   G   +P++NE T+PKF +++R+EK+V+R  N ++KLFSGTANPALSQEIA YMG+ELGK++IKRFADGEIYVQL+ESVRGCDV+LVQPTC P
Sbjct:   42 ICVSGYAKCNLPKALN---GNARVPIINETTIPKFFDSSRLEKSVSRN-NTKLKLFSGTANPALSQEIAWYMGLELGKVSIKRFADGEIYVQLKESVRGCDVFLVQPTCTP 148

HSP 2 Score: 30.4166 bits (67), Expect = 2.197e-38
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDAC 683
            C+P    +ENLMELLIM+DAC
Sbjct:  146 CTPT---NENLMELLIMVDAC 163
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS1_SPIOL (Ribose-phosphate pyrophosphokinase 1 OS=Spinacia oleracea GN=PRS1 PE=2 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 5.908e-32
Identity = 57/86 (66.28%), Postives = 75/86 (87.21%), Query Frame = 2 
Query:  386 LNERTLPKFLETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPPA 643
            +++++ P F+ +   + ++NR  + R+++FSGTANPAL+QEIACYMG++LGKI IKRFADGEIYVQLQESVRGCDV+LVQPTCPPA
Sbjct:    1 MDQQSFPSFMASTHFDSSINRN-DTRLRIFSGTANPALAQEIACYMGLQLGKIKIKRFADGEIYVQLQESVRGCDVFLVQPTCPPA 85

HSP 2 Score: 30.4166 bits (67), Expect = 5.908e-32
Identity = 15/19 (78.95%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  627 PPVLRHENLMELLIMIDAC 683
            PP   +ENLMELLIMIDAC
Sbjct:   83 PPA--NENLMELLIMIDAC 99
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS1_ORYSJ (Ribose-phosphate pyrophosphokinase 1 OS=Oryza sativa subsp. japonica GN=Os02g0127700 PE=2 SV=2)

HSP 1 Score: 125.176 bits (313), Expect = 4.873e-31
Identity = 64/106 (60.38%), Postives = 79/106 (74.53%), Query Frame = 2 
Query:  326 VRCDMAEALNFPNGKPNIPVLNERTLPKFLETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPPA 643
            VRC   + L   NG P    ++ R+L   + T  + +  N   + R+++FSGTANP+LSQEIA Y+G+ELGKINIKRFADGEIYVQLQESVRGCDV+LVQPTCPPA
Sbjct:   35 VRCKKIDQLRAVNGIPPYAPVSNRSLLSPV-TLPIIRDANIKNDTRLRIFSGTANPSLSQEIASYLGLELGKINIKRFADGEIYVQLQESVRGCDVFLVQPTCPPA 139

HSP 2 Score: 30.4166 bits (67), Expect = 4.873e-31
Identity = 15/19 (78.95%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  627 PPVLRHENLMELLIMIDAC 683
            PP   +ENLMELLIMIDAC
Sbjct:  137 PPA--NENLMELLIMIDAC 153
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS5_ARATH (Ribose-phosphate pyrophosphokinase 5 OS=Arabidopsis thaliana GN=PRS5 PE=2 SV=2)

HSP 1 Score: 124.405 bits (311), Expect = 1.076e-30
Identity = 60/107 (56.07%), Postives = 79/107 (73.83%), Query Frame = 2 
Query:  326 VRCDMAEALNFPNGKP-NIPVLNERTLPKFLETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPPA 643
            ++C+ A+   F  G      +L   T+P      R+E ++ + T  R+++FSGTANP L+QEI+CY+G++LGKI IKRFADGEIYVQLQESVRGCDV+LVQPTCPPA
Sbjct:   32 LKCNAADPYKFDGGNSAGFHLLTGDTVPASFSRTRLEDSIYQNTT-RLRIFSGTANPILAQEISCYLGLDLGKIKIKRFADGEIYVQLQESVRGCDVFLVQPTCPPA 137

HSP 2 Score: 30.0314 bits (66), Expect = 1.076e-30
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  627 PPVLRHENLMELLIMIDAC 683
            PP   +ENLMELL+MIDAC
Sbjct:  135 PPA--NENLMELLVMIDAC 151
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS2_ORYSJ (Ribose-phosphate pyrophosphokinase 2 OS=Oryza sativa subsp. japonica GN=Os06g0617800 PE=2 SV=2)

HSP 1 Score: 104.375 bits (259), Expect = 1.266e-24
Identity = 50/75 (66.67%), Postives = 61/75 (81.33%), Query Frame = 2 
Query:  416 ETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            + AR+        ++R+K+FSGTAN  L+QEIA Y+GV+LGK+ IKRFADGEIYVQLQESVRGCDV+LVQPTC P
Sbjct:   74 QEARLAALRTDGRDSRLKIFSGTANRPLAQEIASYLGVDLGKVLIKRFADGEIYVQLQESVRGCDVFLVQPTCSP 148

HSP 2 Score: 29.6462 bits (65), Expect = 1.266e-24
Identity = 14/21 (66.67%), Postives = 16/21 (76.19%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDAC 683
            CSP    +ENLMEL +MIDAC
Sbjct:  146 CSPV---NENLMELFVMIDAC 163
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_PROMM (Ribose-phosphate pyrophosphokinase OS=Prochlorococcus marinus (strain MIT 9313) GN=prs PE=3 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 2.462e-22
Identity = 46/80 (57.50%), Postives = 59/80 (73.75%), Query Frame = 2 
Query:  401 LPKFLETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            +  FL  AR E+        R++LFSGT+NPAL++EIA Y+GV  G    KRFADGE+YVQ+QES+RGCDV+L+QPTC P
Sbjct:    1 MTSFLTAARTEQESISYDMQRLRLFSGTSNPALAREIAAYLGVPDGPRICKRFADGELYVQIQESIRGCDVFLIQPTCAP 80

HSP 2 Score: 28.8758 bits (63), Expect = 2.462e-22
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDAC 683
            C+P    ++NLMELLIM+DAC
Sbjct:   78 CAPV---NDNLMELLIMVDAC 95
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_SYNPX (Ribose-phosphate pyrophosphokinase OS=Synechococcus sp. (strain WH8102) GN=prs PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.194e-21
Identity = 45/80 (56.25%), Postives = 58/80 (72.50%), Query Frame = 2 
Query:  401 LPKFLETARMEKTVNRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            +  FL  AR E+        R++LFSGT+NP L++EIA Y+GV  G    KRFADGE+YVQ+QES+RGCDV+L+QPTC P
Sbjct:    1 MTSFLTAARAEQEKLTPDTRRLRLFSGTSNPGLAREIAAYLGVPDGPRVCKRFADGELYVQIQESIRGCDVFLIQPTCAP 80

HSP 2 Score: 26.9498 bits (58), Expect = 1.194e-21
Identity = 12/21 (57.14%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDAC 683
            C+P    +++LMELLIM+DAC
Sbjct:   78 CAPV---NDHLMELLIMVDAC 95
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_SYNY3 (Ribose-phosphate pyrophosphokinase OS=Synechocystis sp. (strain PCC 6803) GN=prs PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.020e-21
Identity = 39/62 (62.90%), Postives = 55/62 (88.71%), Query Frame = 2 
Query:  455 NNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            NNR++LFSG++NP+LSQE+A Y+G+++G +  KRFADGE+Y+Q+QES+RG DVYL+QP C P
Sbjct:   22 NNRLRLFSGSSNPSLSQEVARYLGMDIGPMLRKRFADGELYIQIQESIRGGDVYLIQPCCHP 83

HSP 2 Score: 30.4166 bits (67), Expect = 2.020e-21
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDAC 683
            C  PV  ++NLMELLIMIDAC
Sbjct:   80 CCHPV--NDNLMELLIMIDAC 98
The following BLAST results are available for this feature:
BLAST of FC931559 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 91
Match NameE-valueIdentityDescription
KPRS1_ARATH3.086e-4780.37Ribose-phosphate pyrophosphokinase 1 OS=Arabidopsi... [more]
KPRS2_SPIOL1.560e-3964.49Ribose-phosphate pyrophosphokinase 2, chloroplasti... [more]
KPRS2_ARATH2.197e-3863.06Ribose-phosphate pyrophosphokinase 2 OS=Arabidopsi... [more]
KPRS1_SPIOL5.908e-3266.28Ribose-phosphate pyrophosphokinase 1 OS=Spinacia o... [more]
KPRS1_ORYSJ4.873e-3160.38Ribose-phosphate pyrophosphokinase 1 OS=Oryza sati... [more]
KPRS5_ARATH1.076e-3056.07Ribose-phosphate pyrophosphokinase 5 OS=Arabidopsi... [more]
KPRS2_ORYSJ1.266e-2466.67Ribose-phosphate pyrophosphokinase 2 OS=Oryza sati... [more]
KPRS_PROMM2.462e-2257.50Ribose-phosphate pyrophosphokinase OS=Prochlorococ... [more]
KPRS_SYNPX1.194e-2156.25Ribose-phosphate pyrophosphokinase OS=Synechococcu... [more]
KPRS_SYNY32.020e-2162.90Ribose-phosphate pyrophosphokinase OS=Synechocysti... [more]

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Properties
Property NameValue
Genbank descriptionC34106E01EF PostHarvP1 Citrus clementina cDNA clone C34106E01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931559 ID=FC931559; Name=FC931559; organism=Citrus clementina; type=EST; length=683bp
GGAAAATCCAAAGCGACGAAGCAAAATAGCCAAAGAAGAAGAAAAAGCAA
CCGAAGCTCTCTCTGCCTCCTCACCGTCACCACAAAAACAAAAGGAAAGA
AAGAAAGAGAGTGAGTGAGAGAAAGAGAGATCAAAAGGAGAGAGAGCTGA
AACAACACAACAGATAACAATGGCTTCGCTGACGATGCCGCGCCCTTCAC
AATCCACGTCAGCTTCTTGCTCTTCGCCTATCCCCAGTCGCTGTTCTTTC
GGTGACCAAAACCCAACTTTTTCATTCAGTTTCAGGGACTCTCGCTCTCG
GGTTCACGTCTGTAATGCCGCCGCCGTTAGATGTGACATGGCTGAGGCAT
TGAATTTTCCAAATGGGAAGCCTAATATTCCAGTTCTAAACGAACGGACG
CTGCCCAAATTTTTGGAAACGGCGCGTATGGAGAAAACTGTCAACAGAAC
TACAAATAACAGGATCAAATTGTTCTCTGGCACCGCCAATCCTGCTCTTT
CCCAGGAAATTGCTTGCTACATGGGCGTGGAACTGGGAAAGATCAACATA
AAGCGATTTGCTGATGGTGAAATTTATGTTCAGCTGCAAGAGAGTGTTAG
AGGATGTGATGTTTATTTGGTGCAGCCCACCTGTCCTCCGGCATGAAAAT
CTCATGGAGCTTTTGATAATGATAGATGCGTGT
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