FC931729

Overview
NameFC931729
Unique NameFC931729
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length771
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig15093 contig Ccv1_Contig15093:13..784. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1X_ARATH (Eukaryotic peptide chain release factor subunit 1-1 OS=Arabidopsis thaliana GN=ERF1-1 PE=2 SV=2)

HSP 1 Score: 299.286 bits (765), Expect = 2.681e-94
Identity = 150/177 (84.75%), Postives = 156/177 (88.14%), Query Frame = 2 
Query:  131 MADGQETDKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            M D  + DKN                RGNGTSMISLIMPPRDQVSRVTKMLGDE+GTASNIKSRVNRQSVLGAITSAQQRLKLYN+VPPNGLVLYTGTIV +DGKEKKVTIDFEPFRPINASLYLCDNKFHT+ALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLP+
Sbjct:    1 MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASNIKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPK 177

HSP 2 Score: 68.1662 bits (165), Expect = 2.681e-94
Identity = 32/35 (91.43%), Postives = 33/35 (94.29%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELAHSF 762
            KKHGRGGQSALRFARLRMEKRHNYVRKTAELA  +
Sbjct:  177 KKHGRGGQSALRFARLRMEKRHNYVRKTAELATQY 211
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1Z_ARATH (Eukaryotic peptide chain release factor subunit 1-3 OS=Arabidopsis thaliana GN=ERF1-3 PE=2 SV=1)

HSP 1 Score: 295.434 bits (755), Expect = 1.724e-93
Identity = 150/177 (84.75%), Postives = 157/177 (88.70%), Query Frame = 2 
Query:  131 MADGQETDKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            MAD QE+DKN                RGNGTSMISLIMPPRDQV+RVTKML DE+GTASNIKSRVNRQSVL AITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPF+PINASLYLCDNKFHT+ LNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLP+
Sbjct:    1 MAD-QESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASNIKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPINASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPK 176

HSP 2 Score: 69.3218 bits (168), Expect = 1.724e-93
Identity = 33/35 (94.29%), Postives = 33/35 (94.29%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELAHSF 762
            KKHGRGGQSALRFARLRMEKRHNYVRKTAELA  F
Sbjct:  176 KKHGRGGQSALRFARLRMEKRHNYVRKTAELATQF 210
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1Y_ARATH (Eukaryotic peptide chain release factor subunit 1-2 OS=Arabidopsis thaliana GN=ERF1-2 PE=2 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 5.465e-92
Identity = 146/173 (84.39%), Postives = 151/173 (87.28%), Query Frame = 2 
Query:  143 QETDKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            +E D N                RGNGTSMISLIMPPRDQVSRVTKMLGDE+GTASNIKSRVNRQSVL AITSAQQRLKLYNKVP NGLVLYTGTIV DDGKEKKVT DFEPFRPINASLYLCDNKFHT+ALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLP+
Sbjct:    3 EEADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASNIKSRVNRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPK 175

HSP 2 Score: 69.3218 bits (168), Expect = 5.465e-92
Identity = 33/35 (94.29%), Postives = 33/35 (94.29%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELAHSF 762
            KKHGRGGQSALRFARLRMEKRHNYVRKTAELA  F
Sbjct:  175 KKHGRGGQSALRFARLRMEKRHNYVRKTAELATQF 209
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1_POLMI (Eukaryotic peptide chain release factor subunit 1 OS=Polyandrocarpa misakiensis GN=ERF1 PE=2 SV=1)

HSP 1 Score: 265.774 bits (678), Expect = 5.511e-83
Identity = 134/178 (75.28%), Postives = 146/178 (82.02%), Query Frame = 2 
Query:  131 MADGQET-DKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            MA+ + T D+N                RGNGTSMISLI+PP+DQVSRV KML DEFGTASNIKSRVNR SVLGAITS QQRLKLYNKVPPNGLV+Y GTIVTD+GKEKKV IDFEPF+PIN SLYLCDNKFHT+AL  LL  D+KFGFIVMDGNG LFGTL GNTREVLHKF+VDLP+
Sbjct:    1 MAETETTADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQVSRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTDEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPK 178

HSP 2 Score: 63.929 bits (154), Expect = 5.511e-83
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELA 753
            KKHGRGGQSALRFARLRMEKRHNYVRK AE A
Sbjct:  178 KKHGRGGQSALRFARLRMEKRHNYVRKVAETA 209
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1_DROME (Eukaryotic peptide chain release factor subunit 1 OS=Drosophila melanogaster GN=eRF1 PE=1 SV=2)

HSP 1 Score: 260.766 bits (665), Expect = 1.025e-81
Identity = 130/176 (73.86%), Postives = 144/176 (81.82%), Query Frame = 2 
Query:  140 GQET--DKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            G+ET  D+N                RGNGTSMISLI+PP+DQ+SRV+KML DEFGTASNIKSRVNR SVLGAITS Q RLKLY KVPPNGLV+Y GTIVT++GKEKKV IDFEPF+PIN SLYLCDNKFHT+AL  LL  D+KFGFIVMDGNG LFGTL GNTREVLHKF+VDLP+
Sbjct:    3 GEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPK 178

HSP 2 Score: 64.6994 bits (156), Expect = 1.025e-81
Identity = 30/32 (93.75%), Postives = 31/32 (96.88%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELA 753
            KKHGRGGQSALRFARLRMEKRHNYVRK AE+A
Sbjct:  178 KKHGRGGQSALRFARLRMEKRHNYVRKVAEVA 209
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1_XENTR (Eukaryotic peptide chain release factor subunit 1 OS=Xenopus tropicalis GN=etf1 PE=2 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 2.966e-81
Identity = 128/175 (73.14%), Postives = 141/175 (80.57%), Query Frame = 2 
Query:  137 DGQETDKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            D    D+N                RGNGTSMISLI+PP+DQ+SRV KML DEFGTASNIKSRVNR SVLGAITS QQRLKLYNKVPPNGLV+Y GTIVT++GKEKKV IDFEPF+PIN SLYLCDNKFHT+AL  LL  D KFGFIV+DG+G LFGTL GNTREVLHKF+VDLP+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPK 178

HSP 2 Score: 63.929 bits (154), Expect = 2.966e-81
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELA 753
            KKHGRGGQSALRFARLRMEKRHNYVRK AE A
Sbjct:  178 KKHGRGGQSALRFARLRMEKRHNYVRKVAETA 209
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1_XENLA (Eukaryotic peptide chain release factor subunit 1 OS=Xenopus laevis GN=etf1 PE=2 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 2.966e-81
Identity = 128/175 (73.14%), Postives = 141/175 (80.57%), Query Frame = 2 
Query:  137 DGQETDKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            D    D+N                RGNGTSMISLI+PP+DQ+SRV KML DEFGTASNIKSRVNR SVLGAITS QQRLKLYNKVPPNGLV+Y GTIVT++GKEKKV IDFEPF+PIN SLYLCDNKFHT+AL  LL  D KFGFIV+DG+G LFGTL GNTREVLHKF+VDLP+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPK 178

HSP 2 Score: 63.929 bits (154), Expect = 2.966e-81
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELA 753
            KKHGRGGQSALRFARLRMEKRHNYVRK AE A
Sbjct:  178 KKHGRGGQSALRFARLRMEKRHNYVRKVAETA 209
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1_RAT (Eukaryotic peptide chain release factor subunit 1 OS=Rattus norvegicus GN=Etf1 PE=2 SV=3)

HSP 1 Score: 259.996 bits (663), Expect = 2.966e-81
Identity = 128/175 (73.14%), Postives = 141/175 (80.57%), Query Frame = 2 
Query:  137 DGQETDKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            D    D+N                RGNGTSMISLI+PP+DQ+SRV KML DEFGTASNIKSRVNR SVLGAITS QQRLKLYNKVPPNGLV+Y GTIVT++GKEKKV IDFEPF+PIN SLYLCDNKFHT+AL  LL  D KFGFIV+DG+G LFGTL GNTREVLHKF+VDLP+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPK 178

HSP 2 Score: 63.929 bits (154), Expect = 2.966e-81
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELA 753
            KKHGRGGQSALRFARLRMEKRHNYVRK AE A
Sbjct:  178 KKHGRGGQSALRFARLRMEKRHNYVRKVAETA 209
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1_RABIT (Eukaryotic peptide chain release factor subunit 1 OS=Oryctolagus cuniculus GN=ETF1 PE=2 SV=3)

HSP 1 Score: 259.996 bits (663), Expect = 2.966e-81
Identity = 128/175 (73.14%), Postives = 141/175 (80.57%), Query Frame = 2 
Query:  137 DGQETDKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            D    D+N                RGNGTSMISLI+PP+DQ+SRV KML DEFGTASNIKSRVNR SVLGAITS QQRLKLYNKVPPNGLV+Y GTIVT++GKEKKV IDFEPF+PIN SLYLCDNKFHT+AL  LL  D KFGFIV+DG+G LFGTL GNTREVLHKF+VDLP+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPK 178

HSP 2 Score: 63.929 bits (154), Expect = 2.966e-81
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELA 753
            KKHGRGGQSALRFARLRMEKRHNYVRK AE A
Sbjct:  178 KKHGRGGQSALRFARLRMEKRHNYVRKVAETA 209
[Close]
BLAST of FC931729 vs. ExPASy Swiss-Prot
Match: ERF1_PONAB (Eukaryotic peptide chain release factor subunit 1 OS=Pongo abelii GN=ETF1 PE=2 SV=3)

HSP 1 Score: 259.996 bits (663), Expect = 2.966e-81
Identity = 128/175 (73.14%), Postives = 141/175 (80.57%), Query Frame = 2 
Query:  137 DGQETDKNXXXXXXXXXXXXXXXXRGNGTSMISLIMPPRDQVSRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFRPINASLYLCDNKFHTDALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPR 661
            D    D+N                RGNGTSMISLI+PP+DQ+SRV KML DEFGTASNIKSRVNR SVLGAITS QQRLKLYNKVPPNGLV+Y GTIVT++GKEKKV IDFEPF+PIN SLYLCDNKFHT+AL  LL  D KFGFIV+DG+G LFGTL GNTREVLHKF+VDLP+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPK 178

HSP 2 Score: 63.929 bits (154), Expect = 2.966e-81
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 1 
Query:  658 KKHGRGGQSALRFARLRMEKRHNYVRKTAELA 753
            KKHGRGGQSALRFARLRMEKRHNYVRK AE A
Sbjct:  178 KKHGRGGQSALRFARLRMEKRHNYVRKVAETA 209
The following BLAST results are available for this feature:
BLAST of FC931729 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 63
Match NameE-valueIdentityDescription
ERF1X_ARATH2.681e-9484.75Eukaryotic peptide chain release factor subunit 1-... [more]
ERF1Z_ARATH1.724e-9384.75Eukaryotic peptide chain release factor subunit 1-... [more]
ERF1Y_ARATH5.465e-9284.39Eukaryotic peptide chain release factor subunit 1-... [more]
ERF1_POLMI5.511e-8375.28Eukaryotic peptide chain release factor subunit 1 ... [more]
ERF1_DROME1.025e-8173.86Eukaryotic peptide chain release factor subunit 1 ... [more]
ERF1_XENTR2.966e-8173.14Eukaryotic peptide chain release factor subunit 1 ... [more]
ERF1_XENLA2.966e-8173.14Eukaryotic peptide chain release factor subunit 1 ... [more]
ERF1_RAT2.966e-8173.14Eukaryotic peptide chain release factor subunit 1 ... [more]
ERF1_RABIT2.966e-8173.14Eukaryotic peptide chain release factor subunit 1 ... [more]
ERF1_PONAB2.966e-8173.14Eukaryotic peptide chain release factor subunit 1 ... [more]

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Properties
Property NameValue
Genbank descriptionC34108C12EF PostHarvP1 Citrus clementina cDNA clone C34108C12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931729 ID=FC931729; Name=FC931729; organism=Citrus clementina; type=EST; length=771bp
GACTTTTATTCTTATTACATCCTCCCTTCTCCTACGAGTTAAGCAAATTC
CATCCTCTTGTTTGTTAGAAACCGATCTGCAATTCTCGGTGCTTTTATCA
CTGTTTTAGAGTTACTGTATCCACTTTACAATGGCAGATGGTCAGGAGAC
TGATAAAAATATTGAGATTTGGAAGATTAAGAAGTTGATCAAAGCACTTG
AAGCTGCTAGAGGAAATGGTACCAGCATGATATCTTTAATCATGCCTCCT
CGTGATCAGGTATCACGAGTCACAAAAATGCTGGGTGATGAGTTTGGAAC
TGCTTCAAACATCAAAAGCAGGGTCAATCGTCAATCTGTATTGGGTGCAA
TCACATCTGCTCAGCAAAGATTGAAGCTTTACAACAAGGTCCCTCCCAAT
GGGCTTGTGCTGTACACGGGAACAATTGTTACTGATGATGGGAAGGAAAA
GAAGGTTACAATTGACTTCGAGCCTTTCAGGCCTATTAATGCATCTCTTT
ACCTCTGTGACAATAAGTTTCACACTGACGCTCTGAATGAACTGTTGGAG
TCTGATGACAAGTTTGGTTTTATTGTCATGGATGGTAATGGAACCCTTTT
TGGGACTCTAAGTGGTAACACCCGTGAGGTGCTTCACAAGTTCAGTGTTG
ACCTTCCAAGAAACATGGAAGAGGTGGACAGTCAGCCCTTCGGTTTGCCC
GACTTCGAATGGAGAAGCGCCATAACTATGTGAGGAAAACAGCTGAACTT
GCACACAGTTTTATATCAATC
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