BG791315

Overview
NameBG791315
Unique NameBG791315
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length226
Libraries
Library NameType
Citrus sinensis flavedo cellscdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig10367 contig Csv1_Contig10367:586..811. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: FTHS_SPIOL (Formate--tetrahydrofolate ligase OS=Spinacia oleracea PE=1 SV=3)

HSP 1 Score: 145.206 bits (365), Expect = 9.863e-35
Identity = 67/75 (89.33%), Postives = 72/75 (96.00%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            EMY+ QGFS LPICMAKTQYSFSHNAAEKGAP+GFILPIRDVR SIGAGFIYPLVGTMSTMPGLPTRPCF++ID+
Sbjct:  552 EMYSKQGFSNLPICMAKTQYSFSHNAAEKGAPSGFILPIRDVRGSIGAGFIYPLVGTMSTMPGLPTRPCFFDIDL 626
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: FTHS_ARATH (Formate--tetrahydrofolate ligase OS=Arabidopsis thaliana GN=THFS PE=2 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.197e-34
Identity = 65/75 (86.67%), Postives = 72/75 (96.00%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            EMYT QGFS LPICM+KTQYSFSH+A++KGAP+GF+LPIRDVR SIGAGFIYPLVGTMSTMPGLPTRPCFYEID+
Sbjct:  549 EMYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVRGSIGAGFIYPLVGTMSTMPGLPTRPCFYEIDI 623
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: C1TC_HUMAN (C-1-tetrahydrofolate synthase, cytoplasmic OS=Homo sapiens GN=MTHFD1 PE=1 SV=3)

HSP 1 Score: 132.88 bits (333), Expect = 5.065e-31
Identity = 59/75 (78.67%), Postives = 66/75 (88.00%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            E+YT QGF  LPICMAKT  S SHN  +KG PTGFILPIRD+RAS+GAGF+YPLVGTMSTMPGLPTRPCFY+ID+
Sbjct:  850 EVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDL 924
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: C1TC_RAT (C-1-tetrahydrofolate synthase, cytoplasmic OS=Rattus norvegicus GN=Mthfd1 PE=1 SV=3)

HSP 1 Score: 132.494 bits (332), Expect = 6.616e-31
Identity = 58/75 (77.33%), Postives = 66/75 (88.00%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            E+YT QGF  LPICMAKT  S SHN  +KG PTGF+LPIRD+RAS+GAGF+YPLVGTMSTMPGLPTRPCFY+ID+
Sbjct:  850 EIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDL 924
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: C1TC_PONAB (C-1-tetrahydrofolate synthase, cytoplasmic OS=Pongo abelii GN=MTHFD1 PE=2 SV=3)

HSP 1 Score: 132.494 bits (332), Expect = 6.616e-31
Identity = 58/75 (77.33%), Postives = 66/75 (88.00%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            E+YT QGF  LP+CMAKT  S SHN  +KG PTGFILPIRD+RAS+GAGF+YPLVGTMSTMPGLPTRPCFY+ID+
Sbjct:  850 EVYTKQGFGNLPVCMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDL 924
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: C1TC_MOUSE (C-1-tetrahydrofolate synthase, cytoplasmic OS=Mus musculus GN=Mthfd1 PE=1 SV=3)

HSP 1 Score: 132.494 bits (332), Expect = 6.616e-31
Identity = 58/75 (77.33%), Postives = 66/75 (88.00%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            E+YT QGF  LPICMAKT  S SHN  +KG PTGF+LPIRD+RAS+GAGF+YPLVGTMSTMPGLPTRPCFY+ID+
Sbjct:  850 EIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDL 924
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: C1TM_BOVIN (Monofunctional C1-tetrahydrofolate synthase, mitochondrial OS=Bos taurus GN=MTHFD1L PE=2 SV=2)

HSP 1 Score: 124.79 bits (312), Expect = 1.379e-28
Identity = 58/75 (77.33%), Postives = 62/75 (82.67%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            + YT QGF  LPICMAKT  S SH   +KG P GFILPI DVRASIGAGFIYPLVGTMSTMPGLPTRPCFY+ID+
Sbjct:  890 DRYTEQGFGNLPICMAKTHLSLSHQPDKKGVPKGFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDL 964
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: C1TM_HUMAN (Monofunctional C1-tetrahydrofolate synthase, mitochondrial OS=Homo sapiens GN=MTHFD1L PE=1 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 8.941e-28
Identity = 57/75 (76.00%), Postives = 61/75 (81.33%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            + YT QGF  LPICMAKT  S SH   +KG P  FILPI DVRASIGAGFIYPLVGTMSTMPGLPTRPCFY+ID+
Sbjct:  893 DRYTQQGFGNLPICMAKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDL 967
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: C1TM_MOUSE (Monofunctional C1-tetrahydrofolate synthase, mitochondrial OS=Mus musculus GN=Mthfd1l PE=1 SV=2)

HSP 1 Score: 120.168 bits (300), Expect = 3.398e-27
Identity = 56/75 (74.67%), Postives = 60/75 (80.00%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            + YT QGF  LPICMAKT  S SH   +KG P  F LPI DVRASIGAGFIYPLVGTMSTMPGLPTRPCFY+ID+
Sbjct:  892 DRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFTLPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDL 966
[Close]
BLAST of BG791315 vs. ExPASy Swiss-Prot
Match: C1TC_SCHPO (C-1-tetrahydrofolate synthase, cytoplasmic OS=Schizosaccharomyces pombe GN=SPBC839.16 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 2.977e-23
Identity = 47/75 (62.67%), Postives = 59/75 (78.67%), Query Frame = 2 
Query:    2 EMYTGQGFSGLPICMAKTQYSFSHNAAEKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYEIDV 226
            E +T QG++ LPIC+AKTQYS SH+   KGAPT F +PIRD+R S GAGFIYPL   +ST+PGLPT+P +Y ID+
Sbjct:  853 ETFTKQGYNNLPICIAKTQYSLSHDPDLKGAPTNFTVPIRDMRLSAGAGFIYPLAAAISTIPGLPTKPAYYNIDI 927
The following BLAST results are available for this feature:
BLAST of BG791315 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 275
Match NameE-valueIdentityDescription
FTHS_SPIOL9.863e-3589.33Formate--tetrahydrofolate ligase OS=Spinacia olera... [more]
FTHS_ARATH2.197e-3486.67Formate--tetrahydrofolate ligase OS=Arabidopsis th... [more]
C1TC_HUMAN5.065e-3178.67C-1-tetrahydrofolate synthase, cytoplasmic OS=Homo... [more]
C1TC_RAT6.616e-3177.33C-1-tetrahydrofolate synthase, cytoplasmic OS=Ratt... [more]
C1TC_PONAB6.616e-3177.33C-1-tetrahydrofolate synthase, cytoplasmic OS=Pong... [more]
C1TC_MOUSE6.616e-3177.33C-1-tetrahydrofolate synthase, cytoplasmic OS=Mus ... [more]
C1TM_BOVIN1.379e-2877.33Monofunctional C1-tetrahydrofolate synthase, mitoc... [more]
C1TM_HUMAN8.941e-2876.00Monofunctional C1-tetrahydrofolate synthase, mitoc... [more]
C1TM_MOUSE3.398e-2774.67Monofunctional C1-tetrahydrofolate synthase, mitoc... [more]
C1TC_SCHPO2.977e-2362.67C-1-tetrahydrofolate synthase, cytoplasmic OS=Schi... [more]

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Properties
Property NameValue
Genbank descriptionCs-31d Citrus sinensis flavedo cells Citrus sinensis cDNA clone Cs-31d, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BG791315 ID=BG791315; Name=BG791315; organism=Citrus sinensis; type=EST; length=226bp
CGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATTTGCATGGCAA
AAACCCAGTATTCATTTTCACACAATGCCGCCGAGAAGGGAGCCCCAACT
GGGTTTATCTTACCGATAAGGGATGTAAGAGCTAGCATTGGGGCTGGATT
CATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGGTCTCCCAACAA
GGCCATGCTTTTACGAGATTGATGTC
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