BQ623147

Overview
NameBQ623147
Unique NameBQ623147
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length649
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2169 contig Csv1_Contig2169:897..1543. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_ORYSJ (Elongation factor 1-alpha OS=Oryza sativa subsp. japonica GN=REFA1 PE=2 SV=2)

HSP 1 Score: 294.278 bits (752), Expect = 3.534e-85
Identity = 144/150 (96.00%), Postives = 150/150 (100.00%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            LQEALPGDNVGFNVKNVAVKDLKRG+VASNSKDDPA+EAA+FTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+ELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFS+YPPLGRFAVRDMRQTVAVGVIK+VEKKDPTG
Sbjct:  287 LQEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKNVEKKDPTG 436

HSP 2 Score: 42.3578 bits (98), Expect = 3.534e-85
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TFGPSGLTTEVKSVEMHH ++
Sbjct:  267 TFGPSGLTTEVKSVEMHHEAL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_MAIZE (Elongation factor 1-alpha OS=Zea mays GN=EF1A PE=3 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 3.863e-84
Identity = 143/150 (95.33%), Postives = 148/150 (98.67%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            LQEALPGDNVGFNVKNVAVKDLKRG+VAS SKDDPA+EAA+FTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+ELVTKIDRRSGKELEKEPKFLKNGDAGMVKM+PTKPMVVETFS YPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG
Sbjct:  287 LQEALPGDNVGFNVKNVAVKDLKRGYVASGSKDDPAKEAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKELEKEPKFLKNGDAGMVKMVPTKPMVVETFSQYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 436

HSP 2 Score: 41.2022 bits (95), Expect = 3.863e-84
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TFGP+GLTTEVKSVEMHH ++
Sbjct:  267 TFGPTGLTTEVKSVEMHHEAL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_VICFA (Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 293.893 bits (751), Expect = 5.040e-84
Identity = 143/150 (95.33%), Postives = 149/150 (99.33%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            L EALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPA+EAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+EL+TKIDRRSGKE+EKEPKFLKNGDAGMVKMIPTKPMVVETF++YPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG
Sbjct:  287 LTEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELITKIDRRSGKEIEKEPKFLKNGDAGMVKMIPTKPMVVETFAEYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 436

HSP 2 Score: 38.891 bits (89), Expect = 5.040e-84
Identity = 16/21 (76.19%), Postives = 19/21 (90.48%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P+GLTTEVKSVEMHH ++
Sbjct:  267 TFAPTGLTTEVKSVEMHHEAL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A2_DAUCA (Elongation factor 1-alpha OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.903e-83
Identity = 141/150 (94.00%), Postives = 146/150 (97.33%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            LQEALPGDNVGFNVKNVAVKDLKRG+VASNSKDDPA+EAANFT+QVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+E+ TKIDRRSGKELEKEPKFLKNGDAG VKMIPTKPMVVETF  YPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG
Sbjct:  287 LQEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTAQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEIQTKIDRRSGKELEKEPKFLKNGDAGFVKMIPTKPMVVETFMSYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 436

HSP 2 Score: 42.3578 bits (98), Expect = 1.903e-83
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TFGPSGLTTEVKSVEMHH ++
Sbjct:  267 TFGPSGLTTEVKSVEMHHEAL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_MANES (Elongation factor 1-alpha OS=Manihot esculenta GN=EF1 PE=3 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 2.482e-83
Identity = 142/150 (94.67%), Postives = 147/150 (98.00%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            LQEALPGDNVGFNVKNVAVKDLKRG VASNSKDDPA+EAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+E++TKIDRRSGKELEKEPKFLKNGDAG VKMIPTKPMVVETFS YPPLGRFAVRDMRQTVAVGVIKSVEKKDP+G
Sbjct:  287 LQEALPGDNVGFNVKNVAVKDLKRGIVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEILTKIDRRSGKELEKEPKFLKNGDAGFVKMIPTKPMVVETFSAYPPLGRFAVRDMRQTVAVGVIKSVEKKDPSG 436

HSP 2 Score: 41.2022 bits (95), Expect = 2.482e-83
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TFGP+GLTTEVKSVEMHH ++
Sbjct:  267 TFGPTGLTTEVKSVEMHHEAL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_TOBAC (Elongation factor 1-alpha OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 3.238e-83
Identity = 140/150 (93.33%), Postives = 149/150 (99.33%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPA+ AA+FTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+E++TKIDRRSGKE+EKEPKFLKNGDAGMVKMIPTKPMVVETFS+YPPLGRFAVRDMRQTVAVGVIK+V+KKDPTG
Sbjct:  287 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAKGAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKNVDKKDPTG 436

HSP 2 Score: 41.2022 bits (95), Expect = 3.238e-83
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TFGP+GLTTEVKSVEMHH ++
Sbjct:  267 TFGPTGLTTEVKSVEMHHEAL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana GN=A1 PE=1 SV=1)

HSP 1 Score: 287.345 bits (734), Expect = 2.080e-82
Identity = 140/150 (93.33%), Postives = 147/150 (98.00%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            L EALPGDNVGFNVKNVAVKDLKRG+VASNSKDDPA+ AANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSE++TKIDRRSGKE+EKEPKFLKNGDAGMVKM PTKPMVVETFS+YPPLGRFAVRDMRQTVAVGVIKSV+KKDPTG
Sbjct:  287 LLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVDKKDPTG 436

HSP 2 Score: 40.0466 bits (92), Expect = 2.080e-82
Identity = 17/21 (80.95%), Postives = 19/21 (90.48%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P+GLTTEVKSVEMHH S+
Sbjct:  267 TFAPTGLTTEVKSVEMHHESL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_WHEAT (Elongation factor 1-alpha OS=Triticum aestivum GN=TEF1 PE=2 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 2.080e-82
Identity = 141/150 (94.00%), Postives = 145/150 (96.67%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            L EALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPA+EAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+ELVTKIDRRSGKELE  PKFLKNGDAG+VKMIPTKPMVVETF+ YPPLGRFAVRDMRQTVAVGVIK VEKKDPTG
Sbjct:  287 LLEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKELEALPKFLKNGDAGIVKMIPTKPMVVETFATYPPLGRFAVRDMRQTVAVGVIKGVEKKDPTG 436

HSP 2 Score: 42.3578 bits (98), Expect = 2.080e-82
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TFGP+GLTTEVKSVEMHH S+
Sbjct:  267 TFGPTGLTTEVKSVEMHHESL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_SOLLC (Elongation factor 1-alpha OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.713e-82
Identity = 137/150 (91.33%), Postives = 149/150 (99.33%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            LQEALPGDNVGFNVKNVAVKDLKRG+VASNSKDDPA+ AA+FT+QVIIMNHPGQIGNGYAPVLDCHTSHIAVKF+E++TKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETF++YPPLGRFAVRDMRQTVAVGV+K+V+KKDPTG
Sbjct:  287 LQEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAASFTAQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAEILTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFAEYPPLGRFAVRDMRQTVAVGVVKNVDKKDPTG 436

HSP 2 Score: 41.2022 bits (95), Expect = 2.713e-82
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TFGP+GLTTEVKSVEMHH ++
Sbjct:  267 TFGPTGLTTEVKSVEMHHEAL 287
[Close]
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Match: EF1A_SOYBN (Elongation factor 1-alpha OS=Glycine max GN=TEFS1 PE=3 SV=2)

HSP 1 Score: 285.419 bits (729), Expect = 7.854e-82
Identity = 139/150 (92.67%), Postives = 147/150 (98.00%), Query Frame = 2 
Query:   65 LQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 514
            L EA PGDNVGFNVKNVAVKDLKRG+VASNSKDDPA+EAANFT+QVII+NHPGQIGNGYAPVLDCHTSHIAVKF+EL+TKIDRRSGKELEKEPKFLKNGDAG VKMIPTKPMVVETFS+YPPLGRFAVRDMRQTVAVGVIK+VEKKDPTG
Sbjct:  287 LTEAHPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTAQVIIINHPGQIGNGYAPVLDCHTSHIAVKFAELMTKIDRRSGKELEKEPKFLKNGDAGFVKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKNVEKKDPTG 436

HSP 2 Score: 40.0466 bits (92), Expect = 7.854e-82
Identity = 17/21 (80.95%), Postives = 19/21 (90.48%), Query Frame = 3 
Query:    3 TFGPSGLTTEVKSVEMHHRSV 65
            TF P+GLTTEVKSVEMHH S+
Sbjct:  267 TFAPTGLTTEVKSVEMHHESL 287
The following BLAST results are available for this feature:
BLAST of BQ623147 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A_ORYSJ3.534e-8596.00Elongation factor 1-alpha OS=Oryza sativa subsp. j... [more]
EF1A_MAIZE3.863e-8495.33Elongation factor 1-alpha OS=Zea mays GN=EF1A PE=3... [more]
EF1A_VICFA5.040e-8495.33Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1[more]
EF1A2_DAUCA1.903e-8394.00Elongation factor 1-alpha OS=Daucus carota PE=2 SV... [more]
EF1A_MANES2.482e-8394.67Elongation factor 1-alpha OS=Manihot esculenta GN=... [more]
EF1A_TOBAC3.238e-8393.33Elongation factor 1-alpha OS=Nicotiana tabacum PE=... [more]
EF1A_ARATH2.080e-8293.33Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
EF1A_WHEAT2.080e-8294.00Elongation factor 1-alpha OS=Triticum aestivum GN=... [more]
EF1A_SOLLC2.713e-8291.33Elongation factor 1-alpha OS=Solanum lycopersicum ... [more]
EF1A_SOYBN7.854e-8292.67Elongation factor 1-alpha OS=Glycine max GN=TEFS1 ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00238 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00238 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623147 ID=BQ623147; Name=BQ623147; organism=Citrus sinensis; type=EST; length=649bp
TGACATTTGGTCCCTCTGGATTGACAACTGAGGTTAAGTCTGTTGAGATG
CACCATCGAAGCGTCTGCAGGAGGCCCTTCCGGGTGACAATGTTGGCTTC
AACGTGAAGAACGTTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCTTC
CAACTCGAAGGATGATCCGGCCAGGGAAGCTGCCAACTTCACTTCACAGG
TCATCATCATGAACCATCCTGGCCAGATTGGAAATGGATATGCCCCAGTG
CTTGACTGCCACACCTCCCACATTGCCGTCAAGTTTTCTGAGCTCGTGAC
GAAGATTGATAGGCGATCAGGCAAGGAGCTTGAGAAGGAGCCCAAGTTCT
TGAAGAATGGAGATGCCGGAATGGTTAAGATGATTCCCACCAAGCCCATG
GTTGTTGAGACATTCTCCGACTACCCACCCCTTGGTCGTTTTGCTGTTAG
GGACATGCGTCAGACTGTTGCTGTGGGTGTTATCAAGAGTGTTGAGAAGA
AGGATCCCACAGGTGCCAAGGTCACCAAGTCCGCAGCCAAGAAGAAATGA
GAAATTTTTATGGGTCTGTCTACAATAGTTAGGGGTTATTTTCTGTCTTT
TTTATTTTCTTTTCTGGTTTTGGTTTGCATCAATGTTTCTGCAAACAGA
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