BQ623282

Overview
NameBQ623282
Unique NameBQ623282
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length626
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:361..985. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_SOLSC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Solenostemon scutellarioides GN=MET PE=1 SV=2)

HSP 1 Score: 311.997 bits (798), Expect = 1.582e-84
Identity = 154/158 (97.47%), Postives = 155/158 (98.10%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 475
            ALREGLPLRKSEHAFYLDWAVHSFRITN GVQDTTQIHTHMCYSNFNDIIHSII+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKY EVKPAL NMVAAAKLLRTQLASAK
Sbjct:  607 ALREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIINMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYAEVKPALENMVAAAKLLRTQLASAK 764
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_ARATH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Arabidopsis thaliana GN=CIMS PE=1 SV=1)

HSP 1 Score: 307.375 bits (786), Expect = 3.896e-83
Identity = 149/158 (94.30%), Postives = 155/158 (98.10%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 475
            ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQD+TQIHTHMCYS+FNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS+EEIADR+NKMLAVLE NILWVNPDCGLKTRKYTEVKPAL NMV AAKL+R+QLASAK
Sbjct:  608 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLAVLEQNILWVNPDCGLKTRKYTEVKPALKNMVDAAKLIRSQLASAK 765
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_CATRO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus GN=METE PE=2 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 1.637e-81
Identity = 147/158 (93.04%), Postives = 153/158 (96.84%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 475
            ALREGLPLRK+EHAFYLDWAVHSFRITN  +QDTTQIHTHMCYSNFNDIIHSIIDMDADV+TIENSRS EKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVL+TNILWVNPDCGLKTRKY EVKPAL NMV+AAKL+RTQLASAK
Sbjct:  608 ALREGLPLRKAEHAFYLDWAVHSFRITNLPLQDTTQIHTHMCYSNFNDIIHSIIDMDADVMTIENSRSSEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLDTNILWVNPDCGLKTRKYAEVKPALENMVSAAKLIRTQLASAK 765
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_MESCR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum GN=METE PE=2 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 2.001e-79
Identity = 145/158 (91.77%), Postives = 149/158 (94.30%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLASAK 475
            ALREGLPLRKSEH FYL WAVHSFRITN GVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIP TEE+ADRI KMLAVLE+N+LWVNPDCGLKTRKY EV PALSNMVAAAK LR +LASAK
Sbjct:  608 ALREGLPLRKSEHDFYLKWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPPTEELADRIRKMLAVLESNVLWVNPDCGLKTRKYGEVNPALSNMVAAAKQLRQELASAK 765
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_BURS3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia sp. (strain 383) GN=metE PE=3 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 5.678e-50
Identity = 102/154 (66.23%), Postives = 114/154 (74.03%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 463
            ALREGLPLR+++   YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ E I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  611 ALREGLPLRRAQWGEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DNFKYPNEIGPGVYDIHSPNIPTQEHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKTLRNQV 763
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_BURCC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain MC0-3) GN=metE PE=3 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 7.416e-50
Identity = 101/154 (65.58%), Postives = 116/154 (75.32%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 463
            ALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK+LR Q+
Sbjct:  611 ALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMRKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKMLRNQV 763
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_BURCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain HI2424) GN=metE PE=3 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 1.652e-49
Identity = 101/154 (65.58%), Postives = 115/154 (74.68%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 463
            ALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  611 ALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKTLRNQV 763
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_BURCA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain AU 1054) GN=metE PE=3 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 1.652e-49
Identity = 101/154 (65.58%), Postives = 115/154 (74.68%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 463
            ALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  611 ALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAKTLRNQV 763
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_BURCJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metE PE=3 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 2.158e-49
Identity = 101/154 (65.58%), Postives = 115/154 (74.68%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQL 463
            ALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK LR Q+
Sbjct:  611 ALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DDFKYPNEIGPGVYDIHSPNIPTQDHIVGLMRKAAERIPAERLWVNPDCGLKTRQWGEVIPALTNMVAAAKTLRNQV 763
[Close]
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Match: METE_SACD2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=metE PE=3 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 2.818e-49
Identity = 98/155 (63.23%), Postives = 115/155 (74.19%), Query Frame = 2 
Query:    2 ALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRTQLA 466
            ALREGLPLRK +   YLDWAV +F+I+  GV DTTQIHTHMCY+ FNDII +I D+DADVITIE SRS+ +LLS F E   Y   IGPGVYDIHSP IP+TE++   I K    L    LW+NPDCGLKTRK+ +V PAL NMV AAK LR ++A
Sbjct:  612 ALREGLPLRKKDWQNYLDWAVEAFKISTAGVGDTTQIHTHMCYAEFNDIIQAIADLDADVITIETSRSNMELLSAF-EQFNYPNDIGPGVYDIHSPNIPATEQVVGLIKKAAENLPLQRLWINPDCGLKTRKWEDVIPALENMVNAAKQLRAEVA 765
The following BLAST results are available for this feature:
BLAST of BQ623282 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 274
Match NameE-valueIdentityDescription
METE_SOLSC1.582e-8497.475-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ARATH3.896e-8394.305-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_CATRO1.637e-8193.045-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MESCR2.001e-7991.775-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURS35.678e-5066.235-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCC7.416e-5065.585-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCH1.652e-4965.585-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCA1.652e-4965.585-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCJ2.158e-4965.585-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SACD22.818e-4963.235-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00373 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00373 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623282 ID=BQ623282; Name=BQ623282; organism=Citrus sinensis; type=EST; length=626bp
TGCCTTGAGGGAAGGATTGCCTCTTAGGAAATCTGAGCATGCTTTCTACT
TGGACTGGGCAGTCCACTCCTTCAGAATCACCAACTGTGGCGTCCAGGAC
ACTACCCAGATCCACACCCACATGTGCTACTCCAACTTCAACGACATTAT
CCACTCAATTATCGACATGGATGCTGATGTGATCACCATTGAGAACTCTC
GTTCTGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGTCAAGTATGGT
GCTGGAATTGGCCCTGGTGTGTATGACATCCACTCTCCAAGAATACCATC
AACTGAAGAAATTGCTGACAGAATCAACAAGATGCTTGCTGTGCTAGAAA
CCAACATTTTGTGGGTTAACCCTGACTGTGGTCTTAAGACTCGCAAGTAC
ACTGAGGTGAAGCCAGCCCTCTCAAACATGGTCGCTGCTGCCAAGCTACT
CCGCACCCAGCTTGCCAGTGCCAAGTGAGTGGTTCTGGAGATTGGAGGGA
TACTTCCTGAGCCATTGATTTTTTTTTTTTTCACTTTAAACAAAGAAAGA
AAATTCCTGTCTTATTACCTATTCAAATACTGGGGGTCCGGAAGGGATGA
AATTTTCCCTATGCATATATGCACTA
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