BQ623715

Overview
NameBQ623715
Unique NameBQ623715
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length674
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
BQ623715_ssr36 microsatellite BQ623715_ssr36:36..50. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig764 contig Csv1_Contig764:571..1245. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3C_SESIN (Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum indicum GN=FAD7 PE=2 SV=1)

HSP 1 Score: 333.183 bits (853), Expect = 1.629e-102
Identity = 152/176 (86.36%), Postives = 163/176 (92.61%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYW AQ TMFWALFVLGHDCGHGSFSND KLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIY+ LD+AT+ LRF +PFPLLAYP YLWSRSPGK GSHF P+SDLFVP+E+KDVITSTVCWTAM ALLVGLSFV+GP+QLLKLYG+PY   VMWLDLVT
Sbjct:  149 PLYWFAQSTMFWALFVLGHDCGHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKNLDTATKKLRFTLPFPLLAYPIYLWSRSPGKQGSHFHPDSDLFVPNEKKDVITSTVCWTAMLALLVGLSFVIGPVQLLKLYGIPYLGNVMWLDLVT 324

HSP 2 Score: 61.2326 bits (147), Expect = 1.629e-102
Identity = 23/24 (95.83%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHGHE+KLPWYRGKEWSYLRG
Sbjct:  325 YLHHHGHEDKLPWYRGKEWSYLRG 348
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3C_RICCO (Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus communis GN=FAD7A-1 PE=2 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 3.616e-102
Identity = 148/176 (84.09%), Postives = 162/176 (92.05%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYW  QGTMFWALFVLGHDCGHGSFSN+ KLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+++LD+ T+ LRF +PFP+LAYPFYLWSRSPGK GSHF P+S LFVP ERKD+ITST CWTAMAALLV L+F MGP+Q+LKLYG+PYWIFVMWLD VT
Sbjct:  159 PLYWFCQGTMFWALFVLGHDCGHGSFSNNPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFKSLDNVTKTLRFSLPFPMLAYPFYLWSRSPGKKGSHFHPDSGLFVPKERKDIITSTACWTAMAALLVYLNFSMGPVQMLKLYGIPYWIFVMWLDFVT 334

HSP 2 Score: 58.9214 bits (141), Expect = 3.616e-102
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHGHE+KLPWYRGK WSYLRG
Sbjct:  335 YLHHHGHEDKLPWYRGKAWSYLRG 358
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3C_ARATH (Omega-3 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana GN=FAD7 PE=1 SV=1)

HSP 1 Score: 330.102 bits (845), Expect = 1.367e-101
Identity = 146/176 (82.95%), Postives = 159/176 (90.34%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYW AQGTMFWALFVLGHDCGHGSFSND KLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHP+SEKIY TLD  TRF RF +P  +LAYPFYLW+RSPGK GSH+ P+SDLF+P ERKDV+TST CWTAMAALLV L+F +GP+Q+LKLYG+PYWI VMWLD VT
Sbjct:  145 PLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINVMWLDFVT 320

HSP 2 Score: 61.2326 bits (147), Expect = 1.367e-101
Identity = 23/24 (95.83%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHGHE+KLPWYRGKEWSYLRG
Sbjct:  321 YLHHHGHEDKLPWYRGKEWSYLRG 344
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3C_SOYBN (Omega-3 fatty acid desaturase, chloroplastic OS=Glycine max GN=FAD7 PE=2 SV=1)

HSP 1 Score: 329.717 bits (844), Expect = 1.784e-101
Identity = 149/176 (84.66%), Postives = 162/176 (92.05%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYWAAQGTMFWALFVLGHDCGHGSFSN+ KLNSVVGHLLHSSILVPYHGWRISHRTHHQ+HGH ENDESWHPL EK++R+LD+ TR LRF  PFPLLA+P YL+SRSPGK+GSHFDP+SDLFVP+ERKDVITST CW AM  LLVGL FVMGP+QLLKLYG+PY IFVMWLDLVT
Sbjct:  153 PLYWAAQGTMFWALFVLGHDCGHGSFSNNSKLNSVVGHLLHSSILVPYHGWRISHRTHHQHHGHAENDESWHPLPEKLFRSLDTVTRMLRFTAPFPLLAFPVYLFSRSPGKTGSHFDPSSDLFVPNERKDVITSTACWAAMLGLLVGLGFVMGPIQLLKLYGVPYVIFVMWLDLVT 328

HSP 2 Score: 61.2326 bits (147), Expect = 1.784e-101
Identity = 23/24 (95.83%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHGHE+KLPWYRGKEWSYLRG
Sbjct:  329 YLHHHGHEDKLPWYRGKEWSYLRG 352
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3D_ARATH (Temperature-sensitive omega-3 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana GN=FAD8 PE=2 SV=1)

HSP 1 Score: 328.946 bits (842), Expect = 3.037e-101
Identity = 143/176 (81.25%), Postives = 159/176 (90.34%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYW AQGTMFWALFVLGHDCGHGSFSND +LNSV GHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E IY+ L+  T+  RF +PFP+LAYPFYLW+RSPGK GSH+ P+SDLF+P E+KDV+TST CWTAMAALLV L+FVMGP+Q+LKLYG+PYWIFVMWLD VT
Sbjct:  138 PLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVT 313

HSP 2 Score: 61.2326 bits (147), Expect = 3.037e-101
Identity = 23/24 (95.83%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHGHE+KLPWYRGKEWSYLRG
Sbjct:  314 YLHHHGHEDKLPWYRGKEWSYLRG 337
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3C_BRANA (Omega-3 fatty acid desaturase, chloroplastic (Fragment) OS=Brassica napus GN=FAD7 PE=2 SV=1)

HSP 1 Score: 325.865 bits (834), Expect = 2.550e-100
Identity = 144/176 (81.82%), Postives = 158/176 (89.77%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYW AQGTMFWALFVLGHDCGHGSFSND +LNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHP+SEKIY++LD  TRF RF +P  +LAYPFYLW+RSPGK GSH+ P+SDLF+P ER DV+TST CWTAMA LLV L+FVMGPMQ+LKLY +PYWI VMWLD VT
Sbjct:  103 PLYWIAQGTMFWALFVLGHDCGHGSFSNDPRLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPMSEKIYKSLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLFLPKERNDVLTSTACWTAMAVLLVCLNFVMGPMQMLKLYVIPYWINVMWLDFVT 278

HSP 2 Score: 61.2326 bits (147), Expect = 2.550e-100
Identity = 23/24 (95.83%), Postives = 24/24 (100.00%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHGHE+KLPWYRGKEWSYLRG
Sbjct:  279 YLHHHGHEDKLPWYRGKEWSYLRG 302
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3E_PHAAU (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Phaseolus aureus GN=ARG1 PE=2 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 2.601e-88
Identity = 128/176 (72.73%), Postives = 148/176 (84.09%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYW AQGTMFWALFVLGHDCGHGSFSN  KLNS VGH+LHS ILVPY+GWRISHRTHHQNHGHVE DESW PL+EK+Y+ LD  TR LR+  PFP+ AYPFYLW+RSPGK GSHF+P S+LF P ERK V+TST+CW  + ++L+ LS  +GP+ +LKLYG+PY IFVMWLD VT
Sbjct:   79 PLYWPAQGTMFWALFVLGHDCGHGSFSNSSKLNSFVGHILHSLILVPYNGWRISHRTHHQNHGHVEKDESWVPLTEKVYKNLDDMTRMLRYSFPFPIFAYPFYLWNRSPGKEGSHFNPYSNLFSPGERKGVVTSTLCWGIVLSVLLYLSLTIGPIFMLKLYGVPYLIFVMWLDFVT 254

HSP 2 Score: 55.0694 bits (131), Expect = 2.601e-88
Identity = 20/24 (83.33%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHG+ +KLPWYRG+EWSYLRG
Sbjct:  255 YLHHHGYTHKLPWYRGQEWSYLRG 278
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3E_SOYBN (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max GN=FAD3 PE=2 SV=1)

HSP 1 Score: 286.96 bits (733), Expect = 5.774e-88
Identity = 126/175 (72.00%), Postives = 146/175 (83.43%), Query Frame = 3 
Query:   78 LYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            +Y   QGTMFWALFVLGHDCGHGSFS+   LNS+VGH+LHSSILVPYHGWRISHRTHHQNHGH+E DESW PL+EKIY+ LDS TR +RF +PFPL  YP YL+SRSPGK GSHF+P S+LF PSERK +  ST+CW  M +LL+ LSF+  P+ +LKLYG+PYWIFVMWLD VT
Sbjct:   83 IYCPIQGTMFWALFVLGHDCGHGSFSDSPLLNSLVGHILHSSILVPYHGWRISHRTHHQNHGHIEKDESWVPLTEKIYKNLDSMTRLIRFTVPFPLFVYPIYLFSRSPGKEGSHFNPYSNLFPPSERKGIAISTLCWATMFSLLIYLSFITSPLLVLKLYGIPYWIFVMWLDFVT 257

HSP 2 Score: 58.9214 bits (141), Expect = 5.774e-88
Identity = 22/24 (91.67%), Postives = 22/24 (91.67%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHGH  KLPWYRGKEWSYLRG
Sbjct:  258 YLHHHGHHQKLPWYRGKEWSYLRG 281
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3E_BRANA (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Brassica napus GN=FAD3 PE=2 SV=1)

HSP 1 Score: 285.419 bits (729), Expect = 9.825e-88
Identity = 127/176 (72.16%), Postives = 146/176 (82.95%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYW AQGT+FWA+FVLGHDCGHGSFS+   LNSVVGH+LHS ILVPYHGWRISHRTHHQNHGHVENDESW PL EK+Y+ L  +TR LR+ +P P+LAYP YLW RSPGK GSHF+P S LF PSERK + TST CW+ M A LV LSF++ P+ +LK+YG+PY IFVMWLD VT
Sbjct:   80 PLYWVAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPEKLYKNLPHSTRMLRYTVPLPMLAYPIYLWYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMLATLVYLSFLVDPVTVLKVYGVPYIIFVMWLDAVT 255

HSP 2 Score: 59.6918 bits (143), Expect = 9.825e-88
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHGH+ KLPWYRGKEWSYLRG
Sbjct:  256 YLHHHGHDEKLPWYRGKEWSYLRG 279
[Close]
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Match: FAD3E_TOBAC (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum GN=FAD3 PE=2 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 1.672e-87
Identity = 127/176 (72.16%), Postives = 147/176 (83.52%), Query Frame = 3 
Query:   75 PLYWAAQGTMFWALFVLGHDCGHGSFSNDHKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRTLDSATRFLRFKIPFPLLAYPFYLWSRSPGKSGSHFDPNSDLFVPSERKDVITSTVCWTAMAALLVGLSFVMGPMQLLKLYGLPYWIFVMWLDLVT 602
            PLYWA QGTMFWA+FVLGHDCGHGSFS+   LN+VVGH+LHS+ILVPYHGWRISH+THHQNHG+VE DESW P+ EK+Y  +  +T+FLR+KIPFPLLAYP YL  RSPGKSGSHF+P SDLF P ERK V+TST+CWT MAALL+ L    G +Q+ K+YG PY IFVMWLD VT
Sbjct:   79 PLYWAIQGTMFWAIFVLGHDCGHGSFSDSQLLNNVVGHILHSAILVPYHGWRISHKTHHQNHGNVETDESWVPMPEKLYNKVGYSTKFLRYKIPFPLLAYPMYLMKRSPGKSGSHFNPYSDLFQPHERKYVVTSTLCWTVMAALLLYLCTAFGSLQMFKIYGAPYLIFVMWLDFVT 254

HSP 2 Score: 58.5362 bits (140), Expect = 1.672e-87
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 2 
Query:  602 YLHHHGHENKLPWYRGKEWSYLRG 673
            YLHHHG+E KLPWYRGKEWSYLRG
Sbjct:  255 YLHHHGYEKKLPWYRGKEWSYLRG 278
The following BLAST results are available for this feature:
BLAST of BQ623715 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 24
Match NameE-valueIdentityDescription
FAD3C_SESIN1.629e-10286.36Omega-3 fatty acid desaturase, chloroplastic OS=Se... [more]
FAD3C_RICCO3.616e-10284.09Omega-3 fatty acid desaturase, chloroplastic OS=Ri... [more]
FAD3C_ARATH1.367e-10182.95Omega-3 fatty acid desaturase, chloroplastic OS=Ar... [more]
FAD3C_SOYBN1.784e-10184.66Omega-3 fatty acid desaturase, chloroplastic OS=Gl... [more]
FAD3D_ARATH3.037e-10181.25Temperature-sensitive omega-3 fatty acid desaturas... [more]
FAD3C_BRANA2.550e-10081.82Omega-3 fatty acid desaturase, chloroplastic (Frag... [more]
FAD3E_PHAAU2.601e-8872.73Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3E_SOYBN5.774e-8872.00Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3E_BRANA9.825e-8872.16Omega-3 fatty acid desaturase, endoplasmic reticul... [more]
FAD3E_TOBAC1.672e-8772.16Omega-3 fatty acid desaturase, endoplasmic reticul... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00806 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00806 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623715 ID=BQ623715; Name=BQ623715; organism=Citrus sinensis; type=EST; length=674bp
ATGTTGTTAGAGATGTTGTGGTGGTTTTTGGCTTGGCTGCTGCTGCTGCT
TATTTGAACAATTGGGTTGTTTGGCCTTTGTACTGGGCTGCTCAGGGAAC
CATGTTTTGGGCTCTTTTTGTGCTCGGTCATGATTGTGGTCATGGAAGCT
TTTCAAATGATCACAAGTTGAATAGTGTGGTGGGGCATCTGCTGCATTCT
TCAATTCTGGTTCCTTATCATGGATGGAGAATTAGCCACAGGACTCATCA
TCAAAACCATGGGCATGTTGAGAATGATGAATCCTGGCACCCGTTGTCTG
AGAAAATTTACAGGACTTTGGATAGTGCAACAAGATTTTTGAGATTCAAA
ATTCCTTTTCCCTTGCTTGCATATCCCTTTTATCTTTGGAGTAGGAGTCC
GGGGAAGTCTGGTTCTCACTTTGACCCTAACAGTGACCTGTTTGTCCCAA
GTGAGAGGAAGGATGTGATCACTTCAACAGTATGCTGGACGGCCATGGCT
GCTTTGCTAGTGGGTTTATCATTTGTAATGGGTCCTATGCAGTTGCTTAA
ACTCTATGGCCTTCCATATTGGATTTTTGTCATGTGGCTGGACCTTGTGA
CTACTTGCATCACCATGGGCATGAAAACAAACTTCCGTGGTATCGGGGAA
AGGAATGGAGTTACTTAAGGGGAG
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