BQ623738

Overview
NameBQ623738
Unique NameBQ623738
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length640
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1387 contig Csv1_Contig1387:78..716. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAHC_TOBAC (Carbonic anhydrase, chloroplastic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 3.186e-57
Identity = 121/205 (59.02%), Postives = 141/205 (68.78%), Query Frame = 1 
Query:   10 NWCLTSVSQAQSCLIKSSTLRPSIVARLN---SPASPPSLIRNEPLFAAPAPIINPNWREDMANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQTPSDTKAFDSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNIV 615
            N CLT +S AQ+ L K +  RP   ARL+   S  S PSLIRNEP+FAAP PIINP  RE+MA +SY                  P+AAA+V+QITA+LQ+   +K FD VE +K GFIHFK EKYEKNPALY EL+KGQSPK MVFACSDSRVC SHVL+FQPGEAFVVRN+AN+VP YD+T+            LHLKV NIV
Sbjct:    7 NSCLT-ISPAQASLKKPT--RPVAFARLSNSSSSTSVPSLIRNEPVFAAPTPIINPILREEMAKESYEQAIAALEKLLSEKGELGPIAAARVDQITAELQSSDGSKPFDPVEHMKAGFIHFKTEKYEKNPALYGELSKGQSPKFMVFACSDSRVCPSHVLNFQPGEAFVVRNIANMVPAYDKTRYSGVGAAIEYAVLHLKVENIV 208

HSP 2 Score: 22.7126 bits (47), Expect = 3.186e-57
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + + + VIGHSACG
Sbjct:  203 KVENIVVIGHSACG 216
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAHC_ARATH (Carbonic anhydrase, chloroplastic OS=Arabidopsis thaliana GN=CA1 PE=1 SV=2)

HSP 1 Score: 215.698 bits (548), Expect = 4.516e-56
Identity = 123/218 (56.42%), Postives = 140/218 (64.22%), Query Frame = 1 
Query:    4 INNWCLTSVSQAQSCLIKSSTLRPSIVARL------------NSPASPPSLIRNEPLFAAPAPIINPNWREDMANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQT--PSDTKAFDSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNIV 615
            ++ + LTS+S +QS L K S    S VA L            +S  S P+LIRNEP+FAAPAPII P W E+M  ++Y                   VAAAKVEQITA LQT   SD KAFD VE IK GFI FK+EKYE NPALY ELAKGQSPK MVFACSDSRVC SHVLDFQPG+AFVVRN+AN+VPP+D+ K            LHLKV NIV
Sbjct:    6 LSGFFLTSLSPSQSSLQKLSLRTSSTVACLPPASSSSSSSSSSSSRSVPTLIRNEPVFAAPAPIIAPYWSEEMGTEAYDEAIEALKKLLIEKEELKTVAAAKVEQITAALQTGTSSDKKAFDPVETIKQGFIKFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFQPGDAFVVRNIANMVPPFDKVKYGGVGAAIEYAVLHLKVENIV 223

HSP 2 Score: 22.7126 bits (47), Expect = 4.516e-56
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + + + VIGHSACG
Sbjct:  218 KVENIVVIGHSACG 231
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAHX_FLABR (Carbonic anhydrase OS=Flaveria brownii PE=2 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 9.083e-54
Identity = 107/173 (61.85%), Postives = 119/173 (68.79%), Query Frame = 1 
Query:   94 NSPASPPSLIRNEPLFAAPAPIINPNWREDMANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQTPSDTKAFDSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNI 612
            +S A+PPSLIRNEP+FAAPAPII PNW ED  N+SY                  PVAAA+++QITAQ   P     FD VERIK GF+ FK EK+  NPALY ELAKGQSPK MVFACSDSRVC SHVLDFQPGEAFVVRNVAN+VPP+D+TK            LHLKV  I
Sbjct:   45 SSSATPPSLIRNEPVFAAPAPIITPNWTED-GNESYEEAIDALKKMLIEKGELEPVAAARIDQITAQAAAPDTKAPFDPVERIKSGFVKFKTEKFVTNPALYDELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANMVPPFDKTKYSGVGAAVEYAVLHLKVQEI 216

HSP 2 Score: 23.0978 bits (48), Expect = 9.083e-54
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + Q ++VIGHS CG
Sbjct:  212 KVQEIFVIGHSRCG 225
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAH1_FLALI (Carbonic anhydrase 1 OS=Flaveria linearis PE=2 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 9.083e-54
Identity = 107/173 (61.85%), Postives = 119/173 (68.79%), Query Frame = 1 
Query:   94 NSPASPPSLIRNEPLFAAPAPIINPNWREDMANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQTPSDTKAFDSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNI 612
            +S A+PPSLIRNEP+FAAPAPII PNW ED  N+SY                  PVAAA+++QITAQ   P     FD VERIK GF+ FK EK+  NPALY ELAKGQSPK MVFACSDSRVC SHVLDFQPGEAFVVRNVAN+VPP+D+TK            LHLKV  I
Sbjct:   45 SSSATPPSLIRNEPVFAAPAPIITPNWTED-GNESYEEAIDALKKMLIEKGELEPVAAARIDQITAQAAAPDTKAPFDPVERIKSGFVKFKTEKFVTNPALYDELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANMVPPFDKTKYSGVGAAVEYAVLHLKVQEI 216

HSP 2 Score: 23.0978 bits (48), Expect = 9.083e-54
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + Q ++VIGHS CG
Sbjct:  212 KVQEIFVIGHSRCG 225
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAHX_FLAPR (Carbonic anhydrase OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 2.623e-53
Identity = 106/173 (61.27%), Postives = 118/173 (68.21%), Query Frame = 1 
Query:   94 NSPASPPSLIRNEPLFAAPAPIINPNWREDMANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQTPSDTKAFDSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNI 612
            +S A+PPSLIRNEP+FAAPAPII PNW ED  N+SY                  PVAAA+++QITAQ   P     FD VERIK GF+ FK EK+  NP LY ELAKGQSPK MVFACSDSRVC SHVLDFQPGEAFVVRNVAN+VPP+D+TK            LHLKV  I
Sbjct:   44 SSSATPPSLIRNEPVFAAPAPIITPNWTED-GNESYEEAIDALKKMLIEKGELEPVAAARIDQITAQAAAPDTKAPFDPVERIKSGFVKFKTEKFVTNPVLYDELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANMVPPFDKTKYSGVGAAVEYAVLHLKVQEI 215

HSP 2 Score: 23.0978 bits (48), Expect = 2.623e-53
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + Q ++VIGHS CG
Sbjct:  211 KVQEIFVIGHSRCG 224
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAHX_FLABI (Carbonic anhydrase OS=Flaveria bidentis PE=2 SV=2)

HSP 1 Score: 206.068 bits (523), Expect = 2.623e-53
Identity = 106/173 (61.27%), Postives = 118/173 (68.21%), Query Frame = 1 
Query:   94 NSPASPPSLIRNEPLFAAPAPIINPNWREDMANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQTPSDTKAFDSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNI 612
            +S A+PPSLIRNEP+FAAPAPII PNW ED  N+SY                  PVAA +++QITAQ   P     FD VERIK GF+ FK EK+  NPALY ELAKGQSPK MVFACSDSRVC SHVLDFQPGEAFVVRNVAN+VPP+D+TK            LHLKV  I
Sbjct:   45 SSSATPPSLIRNEPVFAAPAPIITPNWTED-GNESYEEAIDALKKTLIEKGELEPVAATRIDQITAQAAAPDTKAPFDPVERIKSGFVKFKTEKFVTNPALYDELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANMVPPFDKTKYSGVGAAVEYAVLHLKVQEI 216

HSP 2 Score: 23.0978 bits (48), Expect = 2.623e-53
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + Q ++VIGHS CG
Sbjct:  212 KVQEIFVIGHSRCG 225
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAHC_SPIOL (Carbonic anhydrase, chloroplastic OS=Spinacia oleracea PE=1 SV=2)

HSP 1 Score: 203.756 bits (517), Expect = 1.679e-52
Identity = 113/205 (55.12%), Postives = 138/205 (67.32%), Query Frame = 1 
Query:   10 NWCLTSVSQAQSCLIKSSTLRPSIVA--RLNSPAS-PPSLIRNEPLFAAPAPIINPNWREDMANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQTPSDTKAFDSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNIV 615
            N CLTS+S +++ L  +STLRP+ +A  R+N  +S PPSLIRN+P+FAAPAPII P  +EDMA   Y                    AA+KV QIT++L       A   V+RIK+GFI FK+EKYEKNPALY EL+KGQ+PK MVFACSDSRVC SHVLDFQPGEAF+VRN+AN+VP +D+ K            LHLKV NIV
Sbjct:    5 NGCLTSISPSRTQLKNTSTLRPTFIANSRVNPSSSVPPSLIRNQPVFAAPAPIITPTLKEDMA---YEEAIAALKKLLSEKGELENEAASKVAQITSELADGGTPSASYPVQRIKEGFIKFKKEKYEKNPALYGELSKGQAPKFMVFACSDSRVCPSHVLDFQPGEAFMVRNIANMVPVFDKDKYAGVGAAIEYAVLHLKVENIV 206

HSP 2 Score: 22.7126 bits (47), Expect = 1.679e-52
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + + + VIGHSACG
Sbjct:  201 KVENIVVIGHSACG 214
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAHC_PEA (Carbonic anhydrase, chloroplastic OS=Pisum sativum PE=1 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 1.824e-51
Identity = 116/213 (54.46%), Postives = 140/213 (65.73%), Query Frame = 1 
Query:    1 SINNWCLTSVSQAQSCLIKSSTLRPSIVARLNSPASP-----PSLIRNEPLFAAPAPIINPNWREDMANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQTPSDTKAF---DSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNIV 615
            SIN + L+S+S A++   K +TLRP + A LN+ +S      PSLI+++P+FA+ +PII P  RE+M  + Y                    AA KVEQITAQL T S +      ++ ERIK GF+HFK+EKY+KNPALY ELAKGQSP  MVFACSDSRVC SHVLDFQPGEAFVVRNVAN+VPPYDQ K            LHLKVSNIV
Sbjct:    5 SINGFSLSSLSPAKTST-KRTTLRPFVSASLNTSSSSSSSTFPSLIQDKPVFASSSPIITPVLREEMG-KGYDEAIEELQKLLREKTELKATAAEKVEQITAQLGTTSSSDGIPKSEASERIKTGFLHFKKEKYDKNPALYGELAKGQSPPFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQAKYAGTGAAIEYAVLHLKVSNIV 215

HSP 2 Score: 21.9422 bits (45), Expect = 1.824e-51
Identity = 8/8 (100.00%), Postives = 8/8 (100.00%), Query Frame = 2 
Query:  617 VIGHSACG 640
            VIGHSACG
Sbjct:  216 VIGHSACG 223
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAH2_ARATH (Carbonic anhydrase 2 OS=Arabidopsis thaliana GN=CA2 PE=1 SV=2)

HSP 1 Score: 167.548 bits (423), Expect = 1.098e-41
Identity = 87/146 (59.59%), Postives = 102/146 (69.86%), Query Frame = 1 
Query:  184 MANQSYXXXXXXXXXXXXXXXXXXPVAAAKVEQITAQLQ--TPSDTKAFDSVERIKDGFIHFKREKYEKNPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNIV 615
            M N+SY                   VAAAKV++ITA+LQ  + SD+K+FD VERIK+GF+ FK+EKYE NPALY ELAKGQSPK MVFACSDSRVC SHVLDF PG+AFVVRN+AN+VPP+D+ K            LHLKV NIV
Sbjct:    1 MGNESYEDAIEALKKLLIEKDDLKDVAAAKVKKITAELQAASSSDSKSFDPVERIKEGFVTFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKVENIV 146

HSP 2 Score: 22.7126 bits (47), Expect = 1.098e-41
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + + + VIGHSACG
Sbjct:  141 KVENIVVIGHSACG 154
[Close]
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Match: CAH2_FLALI (Carbonic anhydrase 2 (Fragment) OS=Flaveria linearis PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 8.116e-25
Identity = 54/77 (70.13%), Postives = 58/77 (75.32%), Query Frame = 1 
Query:  385 NPALYSELAKGQSPKNMVFACSDSRVCQSHVLDFQPGEAFVVRNVANIVPPYDQTKXXXXXXXXXXXXLHLKVSNIV 615
            NP LY ELAKGQSPK +VFACSDSRVC SH+LDFQPGEAFVVRN+AN+VPPYD  K            LHLKV NIV
Sbjct:    1 NPTLYGELAKGQSPKFLVFACSDSRVCPSHILDFQPGEAFVVRNIANMVPPYDTIKHSGAGAAIEYAVLHLKVENIV 77

HSP 2 Score: 21.1718 bits (43), Expect = 8.116e-25
Identity = 7/14 (50.00%), Postives = 10/14 (71.43%), Query Frame = 2 
Query:  599 RFQTLWVIGHSACG 640
            + + + VIGHS CG
Sbjct:   72 KVENIVVIGHSCCG 85
The following BLAST results are available for this feature:
BLAST of BQ623738 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 14
Match NameE-valueIdentityDescription
CAHC_TOBAC3.186e-5759.02Carbonic anhydrase, chloroplastic OS=Nicotiana tab... [more]
CAHC_ARATH4.516e-5656.42Carbonic anhydrase, chloroplastic OS=Arabidopsis t... [more]
CAHX_FLABR9.083e-5461.85Carbonic anhydrase OS=Flaveria brownii PE=2 SV=1[more]
CAH1_FLALI9.083e-5461.85Carbonic anhydrase 1 OS=Flaveria linearis PE=2 SV=... [more]
CAHX_FLAPR2.623e-5361.27Carbonic anhydrase OS=Flaveria pringlei PE=2 SV=1[more]
CAHX_FLABI2.623e-5361.27Carbonic anhydrase OS=Flaveria bidentis PE=2 SV=2[more]
CAHC_SPIOL1.679e-5255.12Carbonic anhydrase, chloroplastic OS=Spinacia oler... [more]
CAHC_PEA1.824e-5154.46Carbonic anhydrase, chloroplastic OS=Pisum sativum... [more]
CAH2_ARATH1.098e-4159.59Carbonic anhydrase 2 OS=Arabidopsis thaliana GN=CA... [more]
CAH2_FLALI8.116e-2570.13Carbonic anhydrase 2 (Fragment) OS=Flaveria linear... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00829 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00829 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623738 ID=BQ623738; Name=BQ623738; organism=Citrus sinensis; type=EST; length=640bp
TCGATCAATAACTGGTGCCTTACTTCTGTTTCTCAAGCTCAATCCTGTCT
CATCAAAAGCTCCACATTACGTCCCTCCATCGTTGCAAGGCTCAACTCTC
CTGCTTCTCCTCCCAGCCTCATCCGAAACGAGCCCCTTTTTGCTGCACCA
GCTCCTATCATCAACCCTAACTGGAGGGAAGACATGGCAAATCAGTCGTA
CGAGGAAGCCATTGAAGCTCTCAAGAAACTTCTCAAGGAGAAGGAAGACT
TGAAACCTGTTGCTGCTGCAAAAGTAGAGCAGATTACTGCGCAGCTACAG
ACTCCATCTGACACCAAAGCATTTGATTCCGTGGAGAGGATCAAAGACGG
CTTCATTCACTTCAAGAGAGAAAAATACGAGAAAAATCCAGCTCTGTACT
CTGAGCTTGCCAAAGGCCAAAGCCCGAAAAATATGGTATTTGCCTGCTCA
GACTCCAGGGTTTGCCAGTCCCATGTGTTGGATTTTCAACCTGGGGAAGC
TTTCGTGGTTCGCAATGTTGCTAACATTGTTCCACCCTATGACCAGACTA
AGTATGCTGGAGTTGGAGCTGCTGTCGAGTATGCAGTTTTGCATCTCAAG
GTTTCAAACATTGTGGGTTATTGGGCACAGCGCCTGTGGC
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