BQ623746

Overview
NameBQ623746
Unique NameBQ623746
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length587
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:1015..1600. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_SOLTU (S-adenosylmethionine synthetase 1 OS=Solanum tuberosum GN=METK1 PE=2 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 8.826e-60
Identity = 92/97 (94.85%), Postives = 96/97 (98.97%), Query Frame = 3 
Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 410
            +G ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMM+INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ
Sbjct:  296 SGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMMSINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 392

HSP 2 Score: 53.5286 bits (127), Expect = 8.826e-60
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVA+GL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK_MEDTR (S-adenosylmethionine synthetase OS=Medicago truncatula GN=METK PE=3 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 1.956e-59
Identity = 91/97 (93.81%), Postives = 95/97 (97.94%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL+IVKE+FDFRPGM+TINLDLKRGGN RFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA
Sbjct:  298 GLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKENFDFRPGMITINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 394

HSP 2 Score: 55.0694 bits (131), Expect = 1.956e-59
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAY+VRQAAKSIVANGL
Sbjct:  258 DTYGGWGAHGGGAFSGKDPTKVDRSGAYVVRQAAKSIVANGL 299
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK2_ELAUM (S-adenosylmethionine synthetase 2 OS=Elaeagnus umbellata GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 1.956e-59
Identity = 90/97 (92.78%), Postives = 95/97 (97.94%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRCIVQVSYAIGVP+PLSVFVDSYGTGKIPDKEILKIVKE+FDFRPGM+TINLDLKRGGN RFLKTAAYGHFGRDDPDFTWE+VKPLKWEKPQ+
Sbjct:  297 GLARRCIVQVSYAIGVPDPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMITINLDLKRGGNDRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKPQS 393

HSP 2 Score: 55.4546 bits (132), Expect = 1.956e-59
Identity = 29/42 (69.05%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVANGL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_POPTR (S-adenosylmethionine synthetase 1 OS=Populus trichocarpa GN=METK1 PE=2 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 4.336e-59
Identity = 93/97 (95.88%), Postives = 95/97 (97.94%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMMTINLDLKRGGN RFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA
Sbjct:  297 GLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMMTINLDLKRGGN-RFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 392

HSP 2 Score: 55.4546 bits (132), Expect = 4.336e-59
Identity = 29/42 (69.05%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVANGL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK2_VITVI (S-adenosylmethionine synthetase 2 OS=Vitis vinifera GN=METK2 PE=3 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 5.653e-59
Identity = 90/97 (92.78%), Postives = 95/97 (97.94%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK QA
Sbjct:  297 GLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKTQA 393

HSP 2 Score: 55.4546 bits (132), Expect = 5.653e-59
Identity = 29/42 (69.05%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVANGL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_ELAUM (S-adenosylmethionine synthetase 1 OS=Elaeagnus umbellata GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 7.370e-59
Identity = 88/97 (90.72%), Postives = 94/97 (96.91%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRC+VQVSYAIGVPEPLSVFVDSYGTG+IPDKEIL IVKE+FDFRPGM+TINLDLKRGGNGRFLKTA YGHFGRDDPDFTWE+VKPLKWEKPQ+
Sbjct:  297 GLARRCLVQVSYAIGVPEPLSVFVDSYGTGQIPDKEILNIVKENFDFRPGMITINLDLKRGGNGRFLKTAGYGHFGRDDPDFTWEIVKPLKWEKPQS 393

HSP 2 Score: 55.4546 bits (132), Expect = 7.370e-59
Identity = 29/42 (69.05%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVANGL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK_BRARP (S-adenosylmethionine synthetase OS=Brassica rapa subsp. pekinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 9.609e-59
Identity = 91/97 (93.81%), Postives = 94/97 (96.91%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARR +VQVSYAIGVPEPLSVFVD+YGTG IPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW+KPQA
Sbjct:  297 GMARRALVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393

HSP 2 Score: 54.299 bits (129), Expect = 9.609e-59
Identity = 27/42 (64.29%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKS+VANG+
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGM 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK5_BRAJU (S-adenosylmethionine synthetase 5 OS=Brassica juncea GN=MSAMS5 PE=2 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 9.609e-59
Identity = 91/97 (93.81%), Postives = 94/97 (96.91%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARR +VQVSYAIGVPEPLSVFVD+YGTG IPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW+KPQA
Sbjct:  297 GMARRALVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393

HSP 2 Score: 54.299 bits (129), Expect = 9.609e-59
Identity = 27/42 (64.29%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKS+VANG+
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGM 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK3_BRAJU (S-adenosylmethionine synthetase 3 OS=Brassica juncea GN=MSAMS3 PE=2 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 9.609e-59
Identity = 91/97 (93.81%), Postives = 94/97 (96.91%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARR +VQVSYAIGVPEPLSVFVD+YGTG IPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKW+KPQA
Sbjct:  297 GMARRALVQVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 393

HSP 2 Score: 54.299 bits (129), Expect = 9.609e-59
Identity = 27/42 (64.29%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKS+VANG+
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGM 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK5_VITVI (S-adenosylmethionine synthetase 5 OS=Vitis vinifera GN=METK5 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.253e-58
Identity = 90/94 (95.74%), Postives = 92/94 (97.87%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 404
            G ARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKE+FDFRPGM+ INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK
Sbjct:  297 GLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMIAINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 390

HSP 2 Score: 55.4546 bits (132), Expect = 1.253e-58
Identity = 29/42 (69.05%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVANGL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGL 298
The following BLAST results are available for this feature:
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_SOLTU8.826e-6094.85S-adenosylmethionine synthetase 1 OS=Solanum tuber... [more]
METK_MEDTR1.956e-5993.81S-adenosylmethionine synthetase OS=Medicago trunca... [more]
METK2_ELAUM1.956e-5992.78S-adenosylmethionine synthetase 2 OS=Elaeagnus umb... [more]
METK1_POPTR4.336e-5995.88S-adenosylmethionine synthetase 1 OS=Populus trich... [more]
METK2_VITVI5.653e-5992.78S-adenosylmethionine synthetase 2 OS=Vitis vinifer... [more]
METK1_ELAUM7.370e-5990.72S-adenosylmethionine synthetase 1 OS=Elaeagnus umb... [more]
METK_BRARP9.609e-5993.81S-adenosylmethionine synthetase OS=Brassica rapa s... [more]
METK5_BRAJU9.609e-5993.81S-adenosylmethionine synthetase 5 OS=Brassica junc... [more]
METK3_BRAJU9.609e-5993.81S-adenosylmethionine synthetase 3 OS=Brassica junc... [more]
METK5_VITVI1.253e-5895.74S-adenosylmethionine synthetase 5 OS=Vitis vinifer... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00837 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00837 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623746 ID=BQ623746; Name=BQ623746; organism=Citrus sinensis; type=EST; length=587bp
GGACACTTATGGTGGCTGGGGAGCCCATGGTGGCGGTGCTTTCTCTGGAA
AAGACCCTACTAAGGTGGATAGGAGTGGTGCATACATTGTTAGGCAGGCT
GCTAAGAGCATTGTTGCAAACGGGCTTTGCTCGCAGGTGCATTGTCCAAG
TCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCTGTTTTTGTGGACTCA
TATGGAACCGGCAAGATTCCTGACAAGGAGATCCTCAAAATTGTGAAGGA
AAGCTTTGATTTCAGGCCAGGAATGATGACCATCAATTTGGATCTCAAAA
GGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCCTATGGACACTTTGGC
AGGGATGACCCAGACTTCACTTGGGAAGTTGTGAAGCCCCTCAAGTGGGA
AAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAAAACTATCCCTGCTGT
TCAAGTTTGCTTCTATTGGTAGTGGATGAATAATTTGAATATTATCTTGC
TGCTGCTTTGTAATACTAAGCAAATCTCCTGGGTTCAAAACTCATACATG
TTCCCCATTCAATTCCCTTTCTTATTCTTCTTTCCCC
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