BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig809 contig Csv1_Contig809:454..857. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYN_FLAPR (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.222e-56
Identity = 92/100 (92.00%), Postives = 93/100 (93.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVATTQSA 405
            ENHLVLVNLKNK IDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAY FD AVKLAVKIK E QGTKLKDFVA  QS+
Sbjct:  384 ENHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYLFDLAVKLAVKIKGEAQGTKLKDFVAAMQSS 483

HSP 2 Score: 61.2326 bits (147), Expect = 1.222e-56
Identity = 27/34 (79.41%), Postives = 32/34 (94.12%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T EYKAYQEQV+SNC+KFA+TL++ GYELVSGGT
Sbjct:  350 TAEYKAYQEQVMSNCAKFAETLVKSGYELVSGGT 383
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_SOLTU (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 1.330e-55
Identity = 88/100 (88.00%), Postives = 96/100 (96.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVATTQSA 405
            +NHLVLVN+KNK IDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+EEDF KVA FFDAAVK+AVK+K+ETQGTKLKDFVAT +S+
Sbjct:  384 DNHLVLVNMKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEEDFVKVADFFDAAVKIAVKVKAETQGTKLKDFVATLESS 483

HSP 2 Score: 60.077 bits (144), Expect = 1.330e-55
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPEY+AYQEQVLSN SKFAQ L E GYELVSGGT
Sbjct:  350 TPEYRAYQEQVLSNSSKFAQALGEKGYELVSGGT 383
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_FLAPR (Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 1.446e-54
Identity = 89/100 (89.00%), Postives = 93/100 (93.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVATTQSA 405
            ENHLVLVNLKNK IDGS+VEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFD AVKLAVKIK E +GTKLKDFV   +S+
Sbjct:  384 ENHLVLVNLKNKGIDGSKVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDLAVKLAVKIKGEAKGTKLKDFVTAMESS 483

HSP 2 Score: 57.3806 bits (137), Expect = 1.446e-54
Identity = 26/34 (76.47%), Postives = 31/34 (91.18%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T EYKAYQEQV+SN +KFA+TL++ GYELVSGGT
Sbjct:  350 TAEYKAYQEQVMSNSAKFAETLVKSGYELVSGGT 383
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_ARATH (Serine hydroxymethyltransferase, mitochondrial OS=Arabidopsis thaliana GN=SHM1 PE=1 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 1.006e-52
Identity = 83/100 (83.00%), Postives = 92/100 (92.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVATTQSA 405
            +NHLVLVNLK K IDGSRVEKVLEAVHIA+NKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVA +FD AV +A+K+KSE QGTKLKDFV+  +S+
Sbjct:  383 DNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMESS 482

HSP 2 Score: 61.2326 bits (147), Expect = 1.006e-52
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T EYKAYQEQVLSN +KFAQTL+E GYELVSGGT
Sbjct:  349 TSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGT 382
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_PEA (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum PE=1 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 2.228e-52
Identity = 84/100 (84.00%), Postives = 92/100 (92.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVATTQSA 405
            ENHLVLVNLKNK IDGSRVEKVLE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDF KVA +FDAAV LA+K+K+E++GTKLKDFV   Q++
Sbjct:  384 ENHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFVKVAEYFDAAVSLALKVKAESKGTKLKDFVEALQTS 483

HSP 2 Score: 58.151 bits (139), Expect = 2.228e-52
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPEY+AYQEQVLSN SKFA+ L E GY+LVSGGT
Sbjct:  350 TPEYRAYQEQVLSNSSKFAKALSEKGYDLVSGGT 383
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_CANGA (Serine hydroxymethyltransferase, mitochondrial OS=Candida glabrata GN=SHM1 PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.088e-31
Identity = 50/84 (59.52%), Postives = 70/84 (83.33%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSE 357
            +NHL+L+NL N  IDG+R+E +LE ++IAANKNT+PGD SA+ P G+R+GTPA+T+RGF E+DF KVA + D AVKL++ +KS+
Sbjct:  357 DNHLLLLNLSNMGIDGARLEAILEKINIAANKNTIPGDKSALFPSGLRVGTPAMTTRGFQEQDFKKVAEYIDNAVKLSIALKSQ 440

HSP 2 Score: 45.0542 bits (105), Expect = 1.088e-31
Identity = 20/34 (58.82%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+  YQ+QV+SN   F   LL+ G+ELVSGGT
Sbjct:  323 TPEFVEYQKQVVSNAKAFGDELLKRGFELVSGGT 356
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_CANAL (Serine hydroxymethyltransferase, mitochondrial OS=Candida albicans GN=SHM1 PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.845e-31
Identity = 54/96 (56.25%), Postives = 77/96 (80.21%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVAT 393
            + HL+LV+L+++ IDG+RVE VLE  +IAANKNTVPGDVSA+ P G+R+GTPA+T+RGF  E+F KVA F D AV +A+++K++ QG   K+ +A+
Sbjct:  367 DTHLILVDLRSRNIDGARVEAVLERANIAANKNTVPGDVSALFPSGLRVGTPAMTTRGFGPEEFDKVAEFIDQAVNIAIELKAQEQGKVPKELLAS 462

HSP 2 Score: 40.0466 bits (92), Expect = 1.845e-31
Identity = 17/33 (51.52%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PEY  YQ++V+SN   FA  L+  G++LVS GT
Sbjct:  334 PEYVKYQQEVVSNAKHFADALVSKGFKLVSDGT 366
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_BOVIN (Serine hydroxymethyltransferase, mitochondrial OS=Bos taurus GN=SHMT2 PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 3.128e-31
Identity = 56/93 (60.22%), Postives = 73/93 (78.49%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDF 384
            +NHLVLV+L+ K +DG+R E+VLE V I ANKNT PGD SA+ PGG+R+G PALTSRGF+E+DF KV  F D  V + +++KS+T  TKL+DF
Sbjct:  378 DNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRGFLEDDFRKVVGFIDEGVNIGLEVKSKT--TKLQDF 468

HSP 2 Score: 40.4318 bits (93), Expect = 3.128e-31
Identity = 18/34 (52.94%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TP ++ Y  Q+L N    A  LLE GY LVSGGT
Sbjct:  344 TPMFREYSLQILKNAQAMANALLERGYSLVSGGT 377
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_NEUCR (Putative serine hydroxymethyltransferase, mitochondrial OS=Neurospora crassa GN=NCU05805 PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 5.300e-31
Identity = 56/91 (61.54%), Postives = 72/91 (79.12%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLK 378
            +NHLVL++LK + IDGSRVE+VLE V +AANKNTVPGD SA+ PGG+R+GTPA+T+RGF EEDFA+VA   D AV +AV+I    +   +K
Sbjct:  389 DNHLVLIDLKPQGIDGSRVERVLELVGVAANKNTVPGDKSALTPGGLRIGTPAMTTRGFTEEDFARVADIIDRAVTIAVRINKAAKEDAVK 479

HSP 2 Score: 38.891 bits (89), Expect = 5.300e-31
Identity = 20/40 (50.00%), Postives = 26/40 (65.00%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLE------MGYELVSGGT 104
            TPE++AYQ QVL+N +  A  L E      +GY +VSGGT
Sbjct:  349 TPEFRAYQSQVLANATALAARLGEPKDKNGLGYTIVSGGT 388
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_YEAST (Serine hydroxymethyltransferase, mitochondrial OS=Saccharomyces cerevisiae GN=SHM1 PE=1 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 1.525e-30
Identity = 48/90 (53.33%), Postives = 72/90 (80.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKL 375
            +NHL++++L   ++DG+RVE +L A++IAANKNT+PGD SA+ P G+R+GTPA+T+RGF  E+F++VA + D+AVKLA  +K+    TKL
Sbjct:  362 DNHLIVIDLSGTQVDGARVETILSALNIAANKNTIPGDKSALFPSGLRIGTPAMTTRGFGREEFSQVAKYIDSAVKLAENLKTLEPTTKL 451

HSP 2 Score: 45.8246 bits (107), Expect = 1.525e-30
Identity = 19/34 (55.88%), Postives = 27/34 (79.41%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +PE+K YQ++++ N   FAQ L +MGY+LVSGGT
Sbjct:  328 SPEFKEYQQKIVDNSKWFAQELTKMGYKLVSGGT 361
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYN_FLAPR1.222e-5692.00Serine hydroxymethyltransferase 2, mitochondrial O... [more]
GLYM_SOLTU1.330e-5588.00Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_FLAPR1.446e-5489.00Serine hydroxymethyltransferase 1, mitochondrial O... [more]
GLYM_ARATH1.006e-5283.00Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_PEA2.228e-5284.00Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_CANGA1.088e-3159.52Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_CANAL1.845e-3156.25Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_BOVIN3.128e-3160.22Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_NEUCR5.300e-3161.54Putative serine hydroxymethyltransferase, mitochon... [more]
GLYM_YEAST1.525e-3053.33Serine hydroxymethyltransferase, mitochondrial OS=... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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