Transferability and Level of Heterozygosity of Microsatellite Markers in Citrus Species

Publication Overview
TitleTransferability and Level of Heterozygosity of Microsatellite Markers in Citrus Species
AuthorsCristofani-Yaly M, Novelli VM, Bastianel M, Machado MA
TypeJournal Article
Journal NamePlant molecular biology reporter
CitationCristofani-Yaly M, Novelli VM, Bastianel M, Machado MA. Transferability and Level of Heterozygosity of Microsatellite Markers in Citrus Species. Plant molecular biology reporter. 2011; 29(2):418-423.


Microsatellite markers are a powerful tool for genetic studies, including germplasm conservation, cultivar identification, and integration of linkage maps. Several works have shown that primer pairs designed for one species can be used in related species to facilitate wider application because it reduces the costs for primer development. The objective of this study was to evaluate the transferability of microsatellite primers which was previously developed from the genomic library of Pêra sweet orange (Citrus sinensis L. Osbeck) and to determine the level of heterozygosity between citrus accessions and related genera. Twenty-four microsatellite loci were evaluated on 12 genotypes of Citrus, Poncirus, and an intergeneric hybrid. All analyzed markers were transferable across all genotypes. Seventeen loci were polymorphic, and the number of alleles per loci ranged from one to six. The lowest level of heterozygosity was observed for Poncirus trifoliata (L.) Raf. cultivars while the highest level was for Swingle citrumelo. In general, microsatellite markers showed wide genetic variation and demonstrated that they can be useful in citrus breeding programs.
This publication contains information about 24 features:
Feature NameUniquenameType
CCSM 01CCSM 01genetic_marker
CCSM 03CCSM 03genetic_marker
CCSM 04CCSM 04genetic_marker
CCSM 06CCSM 06genetic_marker
CCSM 09CCSM 09genetic_marker
CCSM 10CCSM 10genetic_marker
CCSM 12CCSM 12genetic_marker
CCSM 13CCSM 13genetic_marker
CCSM 14CCSM 14genetic_marker
CCSM 15CCSM 15genetic_marker
CCSM 17CCSM 17genetic_marker
CCSM 40CCSM 40genetic_marker
CCSM 46CCSM 46genetic_marker
CCSM 68CCSM 68genetic_marker
CCSM 69CCSM 69genetic_marker
CCSM 70CCSM 70genetic_marker
CCSM 75CCSM 75genetic_marker
CCSM 77CCSM 77genetic_marker
CCSM 95CCSM 95genetic_marker
CCSM 111CCSM 111genetic_marker
CCSM 112CCSM 112genetic_marker
CCSM 144CCSM 144genetic_marker
CCSM 146CCSM 146genetic_marker
CCSM 156CCSM 156genetic_marker
This publication contains information about 11 stocks:
Stock NameUniquenameType
Cabo pummeloCabo pummeloaccession
Murcott tangorMurcott tangoraccession
Rangpur limeRangpur limeaccession
Sunki mandarinSunki mandarinaccession
Swingle citrumeloSwingle citrumeloaccession
flying dragonflying dragonaccession
Additional details for this publication include:
Property NameValue
Published Location|||
Publication TypeJournal Article
Publication Date2011
Language Abbreng
Publication Model[electronic resource].
KeywordsCitrus sinensis, Poncirus trifoliata, alleles, chromosome mapping, cultivar identification, cultivars, genetic variation, genomic libraries, germplasm conservation, heterozygosity, hybrids, loci, microsatellite repeats, oranges