CB322149

Overview
NameCB322149
Unique NameCB322149
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length469
Libraries
Library NameType
Mature leaf blade cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig426 contig Csv1_Contig426:164..632. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP71_ARATH (Heat shock cognate 70 kDa protein 1 OS=Arabidopsis thaliana GN=HSC70-1 PE=1 SV=3)

HSP 1 Score: 289.656 bits (740), Expect = 4.291e-78
Identity = 144/155 (92.90%), Postives = 148/155 (95.48%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQHDRVEIIANDQGNRTTPSYV FTD+ERLIGDAAKNQVAMNP NTVFDAKRLIGRRFSD+SVQ DMKLWPFK+ AGPADKPMI V YKGEEK+FAAEEISSMVLIKMREIAEAYLG TIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV
Sbjct:   19 SCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPVNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQAGPADKPMIYVEYKGEEKEFAAEEISSMVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 173
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP7C_PETHY (Heat shock cognate 70 kDa protein OS=Petunia hybrida GN=HSP70 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 9.559e-78
Identity = 144/155 (92.90%), Postives = 146/155 (94.19%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNP NTVFDAKRLIGRRFSD SVQ D+KLWPFKVI GP DKPMI V YKGEEKQFAAEEISSMVL KM+EIAEAYLG TIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV
Sbjct:   19 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDPSVQSDIKLWPFKVIPGPGDKPMIVVTYKGEEKQFAAEEISSMVLTKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 173
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP72_SOLLC (Heat shock cognate 70 kDa protein 2 OS=Solanum lycopersicum GN=HSC-2 PE=2 SV=1)

HSP 1 Score: 287.345 bits (734), Expect = 2.129e-77
Identity = 142/155 (91.61%), Postives = 148/155 (95.48%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQHDRVEIIANDQGNRTTPSYVGFTD+ERLIGDAAKNQVAMNP NTVFDAKRLIGRRFSDASVQ DMKLWPFKVI GP DKPMI VNYKGEEKQF+AEEISSMVLIKM+EIAEA+LG T+KNAVVTVPAY NDSQRQATKDAGVI+GLNV
Sbjct:   19 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDSERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDASVQSDMKLWPFKVIPGPGDKPMIVVNYKGEEKQFSAEEISSMVLIKMKEIAEAFLGTTVKNAVVTVPAYSNDSQRQATKDAGVISGLNV 173
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP71_SOLLC (Heat shock cognate 70 kDa protein 1 OS=Solanum lycopersicum GN=HSC-I PE=2 SV=1)

HSP 1 Score: 286.189 bits (731), Expect = 4.744e-77
Identity = 141/155 (90.97%), Postives = 148/155 (95.48%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVA+NP NTVFDAKRLIGRRFSDASVQ DMKLWPFKVI GP DKPMI V YKGEEK+FAAEEISSMVL KM+EIAEA+LG+T+KNAVVTVPAYFNDSQRQATKDAGVI+GLNV
Sbjct:   19 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVALNPINTVFDAKRLIGRRFSDASVQEDMKLWPFKVIPGPGDKPMIVVTYKGEEKEFAAEEISSMVLTKMKEIAEAFLGSTVKNAVVTVPAYFNDSQRQATKDAGVISGLNV 173
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP72_ARATH (Heat shock cognate 70 kDa protein 2 OS=Arabidopsis thaliana GN=HSC70-2 PE=2 SV=2)

HSP 1 Score: 280.796 bits (717), Expect = 1.993e-75
Identity = 139/155 (89.68%), Postives = 146/155 (94.19%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQHDRVEIIANDQGNRTTPSYV FTD+ERLIGDAAKNQVAMNP NTVFDAKRLIGRRFSDASVQ D +LWPF +I+G A+KPMI V YKGEEKQFAAEEISSMVLIKMREIAEA+LG T+KNAVVTVPAYFNDSQRQATKDAGVIAGLNV
Sbjct:   19 SCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPVNTVFDAKRLIGRRFSDASVQSDRQLWPFTIISGTAEKPMIVVEYKGEEKQFAAEEISSMVLIKMREIAEAFLGTTVKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 173
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP73_ARATH (Heat shock cognate 70 kDa protein 3 OS=Arabidopsis thaliana GN=HSC70-3 PE=1 SV=1)

HSP 1 Score: 280.411 bits (716), Expect = 2.603e-75
Identity = 137/155 (88.39%), Postives = 148/155 (95.48%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQHDRVEIIANDQGNRTTPSYV FTD+ERLIGDAAKNQVAMNP NTVFDAKRLIGRRF+D+SVQ D+KLWPF + +GPA+KPMI VNYKGE+K+F+AEEISSM+LIKMREIAEAYLG TIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV
Sbjct:   19 SCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPINTVFDAKRLIGRRFTDSSVQSDIKLWPFTLKSGPAEKPMIVVNYKGEDKEFSAEEISSMILIKMREIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 173
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP7E_SPIOL (Chloroplast envelope membrane 70 kDa heat shock-related protein OS=Spinacia oleracea GN=SCE70 PE=1 SV=3)

HSP 1 Score: 278.87 bits (712), Expect = 7.574e-75
Identity = 139/155 (89.68%), Postives = 147/155 (94.84%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            S VGVWQHDRVEIIANDQGNRTTPSYV FTD+ERLIGDAAKNQVAMNP NTVFDAKRLIGRRFSDASVQ DMK  PFKV++GP +KPMIGVNYKGEEKQFAAEEISSMVL KM+EIAEAYLG+T+KNAVVTVPAYFNDSQRQATKDAGVI+GLNV
Sbjct:   19 SRVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDASVQADMKHRPFKVVSGPGEKPMIGVNYKGEEKQFAAEEISSMVLTKMKEIAEAYLGSTVKNAVVTVPAYFNDSQRQATKDAGVISGLNV 173
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_SOYBN (Heat shock 70 kDa protein OS=Glycine max GN=HSP70 PE=3 SV=1)

HSP 1 Score: 273.478 bits (698), Expect = 3.182e-73
Identity = 134/155 (86.45%), Postives = 143/155 (92.26%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQ+DRVEII NDQGNRTTPSYV FTDTERLIGDAAKNQVAMNP NTVFDAKRLIGRRFSD+SVQ DMKLWPFKV   P DKPMI VNYKGEEK+F+AEEISSMVL+KMRE+AEA+LG  +KNAVVTVPAYFNDSQRQATKDAG I+GLNV
Sbjct:   18 SCVGVWQNDRVEIIPNDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVFDAKRLIGRRFSDSSVQNDMKLWPFKVGGSPCDKPMIVVNYKGEEKKFSAEEISSMVLVKMREVAEAFLGHAVKNAVVTVPAYFNDSQRQATKDAGAISGLNV 172
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_MAIZE (Heat shock 70 kDa protein OS=Zea mays GN=HSP70 PE=3 SV=2)

HSP 1 Score: 271.166 bits (692), Expect = 1.579e-72
Identity = 138/155 (89.03%), Postives = 143/155 (92.26%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQHDRVEIIANDQGNRTTPSYV FTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFS  +VQ  MKLWP + + G  DKPMI  NYKGEEKQFAAEEISSMVLIKM+EIAEAYLG+TIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV
Sbjct:   18 SCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSSPAVQSSMKLWPSRHL-GLGDKPMIVFNYKGEEKQFAAEEISSMVLIKMKEIAEAYLGSTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 171
[Close]
BLAST of CB322149 vs. ExPASy Swiss-Prot
Match: HSP70_DAUCA (Heat shock 70 kDa protein OS=Daucus carota GN=HSP70 PE=3 SV=1)

HSP 1 Score: 257.684 bits (657), Expect = 1.807e-68
Identity = 130/155 (83.87%), Postives = 139/155 (89.68%), Query Frame = 3 
Query:    3 SCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDASVQGDMKLWPFKVIAGPADKPMIGVNYKGEEKQFAAEEISSMVLIKMREIAEAYLGATIKNAVVTVPAYFNDSQRQATKDAGVIAGLNV 467
            SCVGVWQ+DRVEIIANDQGNRTTPSY  FTDT RLIGDA KNQVAMNP+NTVFDAKRLIGRRF+  SVQ DMKLWP +VI GP +KPMI VNYKGE KQFAAEEISSMVLIKM EIAEA+LG ++ +AVVTVPAYFNDSQRQATKD GVIAGLNV
Sbjct:   18 SCVGVWQNDRVEIIANDQGNRTTPSYFAFTDTSRLIGDA-KNQVAMNPSNTVFDAKRLIGRRFNHPSVQSDMKLWPLQVIPGPGEKPMIVVNYKGESKQFAAEEISSMVLIKMLEIAEAFLGHSVNDAVVTVPAYFNDSQRQATKDTGVIAGLNV 171
The following BLAST results are available for this feature:
BLAST of CB322149 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HSP71_ARATH4.291e-7892.90Heat shock cognate 70 kDa protein 1 OS=Arabidopsis... [more]
HSP7C_PETHY9.559e-7892.90Heat shock cognate 70 kDa protein OS=Petunia hybri... [more]
HSP72_SOLLC2.129e-7791.61Heat shock cognate 70 kDa protein 2 OS=Solanum lyc... [more]
HSP71_SOLLC4.744e-7790.97Heat shock cognate 70 kDa protein 1 OS=Solanum lyc... [more]
HSP72_ARATH1.993e-7589.68Heat shock cognate 70 kDa protein 2 OS=Arabidopsis... [more]
HSP73_ARATH2.603e-7588.39Heat shock cognate 70 kDa protein 3 OS=Arabidopsis... [more]
HSP7E_SPIOL7.574e-7589.68Chloroplast envelope membrane 70 kDa heat shock-re... [more]
HSP70_SOYBN3.182e-7386.45Heat shock 70 kDa protein OS=Glycine max GN=HSP70 ... [more]
HSP70_MAIZE1.579e-7289.03Heat shock 70 kDa protein OS=Zea mays GN=HSP70 PE=... [more]
HSP70_DAUCA1.807e-6883.87Heat shock 70 kDa protein OS=Daucus carota GN=HSP7... [more]

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Properties
Property NameValue
Genbank descriptionEST0265 Mature leaf blade cDNA subtraction library Citrus sinensis cDNA clone 24LB184 similar to HSP70 (acc# AAB88009), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB322149 ID=CB322149; Name=CB322149; organism=Citrus sinensis; type=EST; length=469bp
ACTCCTGCGTCGGAGTCTGGCAGCATGACAGAGTCGAGATTATCGCCAAT
GACCAAGGGAACCGGACGACGCCGTCTTATGTGGGGTTCACCGACACGGA
GCGTTTGATCGGGGACGCTGCTAAGAACCAGGTCGCCATGAACCCCACGA
ACACCGTCTTTGATGCAAAGAGGTTGATTGGTAGGAGATTCAGCGATGCA
TCAGTCCAAGGCGATATGAAGCTATGGCCATTTAAGGTGATTGCTGGACC
TGCTGACAAGCCCATGATTGGTGTTAACTACAAGGGCGAAGAGAAGCAGT
TTGCTGCTGAAGAAATCTCGTCTATGGTGCTCATCAAGATGCGTGAGATT
GCTGAGGCTTACCTTGGCGCAACTATTAAGAATGCAGTCGTTACTGTCCC
TGCTTACTTCAATGACTCACAGAGGCAGGCCACCAAGGATGCCGGTGTCA
TTGCTGGGCTTAATGTGAT
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