CF417605 | Citrus Genome Database

Overview


Name	CF417605
Unique Name	CF417605
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	646

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig9884	contig	Csv1_Contig9884:45..689.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB23_APIGR (Chlorophyll a-b binding protein, chloroplastic OS=Apium graveolens GN=LHC0 PE=1 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 1.823e-90
Identity = 132/156 (84.62%), Postives = 140/156 (89.74%), Query Frame = 3

Query:   69 GKAVNLAPSGNELSNGGRISMRKTGSKSVSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            GKAV +APS +EL   GR+SMRKT    VS  SPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIH RWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSIL+IWA QV+L G+
Sbjct:   15 GKAVKVAPSSSELFGNGRVSMRKTVKAPVSD-SPWYGPDRVKYLGPFSGEAPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHSRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILSIWATQVILMGA 169

HSP 2 Score: 77.7962 bits (190), Expect = 1.823e-90
Identity = 33/37 (89.19%), Postives = 36/37 (97.30%), Query Frame = 1

Query:  529 MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPE 639
            MGAVEGYR+AGGPLGE+ DPLYPGGSFDPLGLA+DPE
Sbjct:  167 MGAVEGYRVAGGPLGEIVDPLYPGGSFDPLGLAEDPE 203

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB24_TOBAC (Chlorophyll a-b binding protein 40, chloroplastic OS=Nicotiana tabacum GN=CAB40 PE=2 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 3.626e-79
Identity = 138/160 (86.25%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   63 FVGKAVNLAPSGNELSNGGRISMRKTGSKS--VSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            F GKAV L+PS +E++  G+++MRKT SK+  VSSGSPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQVVL G+
Sbjct:   13 FAGKAVKLSPSSSEITGNGKVTMRKTASKAKTVSSGSPWYGPDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGA 172

HSP 2 Score: 82.4185 bits (202), Expect = 2.187e-15
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGAVEGYR+AGGPLGEV DPLYPGGSFDPLGLA+DPEAF
Sbjct:  160 LAIWACQVVLMGAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEAF 208

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB21_TOBAC (Chlorophyll a-b binding protein 16, chloroplastic OS=Nicotiana tabacum GN=CAB16 PE=2 SV=1)

HSP 1 Score: 293.893 bits (751), Expect = 4.736e-79
Identity = 138/160 (86.25%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   63 FVGKAVNLAPSGNELSNGGRISMRKTGSKS--VSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            F GKAV L+PS +E++  G+++MRKT SK+  VSSGSPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQVVL G+
Sbjct:   12 FAGKAVKLSPSSSEVTGNGKVTMRKTASKAKPVSSGSPWYGPDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGA 171

HSP 2 Score: 82.4185 bits (202), Expect = 2.187e-15
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGAVEGYR+AGGPLGEV DPLYPGGSFDPLGLA+DPEAF
Sbjct:  159 LAIWACQVVLMGAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEAF 207

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB2G_SOLLC (Chlorophyll a-b binding protein 3C, chloroplastic OS=Solanum lycopersicum GN=CAB3C PE=3 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.055e-78
Identity = 137/160 (85.62%), Postives = 148/160 (92.50%), Query Frame = 3

Query:   63 FVGKAVNLAPSGNELSNGGRISMRKTGSKS--VSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            F GKAV L+PS +E++  GR++MRKT +K+   SSGSPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQVVL G+
Sbjct:   13 FAGKAVKLSPSSSEITGNGRVTMRKTATKAKPASSGSPWYGPDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGA 172

HSP 2 Score: 84.3445 bits (207), Expect = 5.755e-16
Identity = 41/49 (83.67%), Postives = 42/49 (85.71%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGAVEGYRIAGGPLGEV DPLYPGGSFDPLGLADDPEAF
Sbjct:  160 LAIWACQVVLMGAVEGYRIAGGPLGEVVDPLYPGGSFDPLGLADDPEAF 208

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB25_NICPL (Chlorophyll a-b binding protein E, chloroplastic OS=Nicotiana plumbaginifolia GN=CABE PE=3 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.055e-78
Identity = 137/160 (85.62%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   63 FVGKAVNLAPSGNELSNGGRISMRKTGSKS--VSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            F GKAV L+PS +E++  G+++MRKT +K+  VSSGSPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQVVL G+
Sbjct:   12 FAGKAVKLSPSSSEVTGNGKVTMRKTANKAKPVSSGSPWYGPDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGA 171

HSP 2 Score: 79.337 bits (194), Expect = 1.851e-14
Identity = 38/49 (77.55%), Postives = 41/49 (83.67%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGAVEGYR+AG PLGEV DPLYPGGSFDPLGLA+DPEAF
Sbjct:  159 LAIWACQVVLMGAVEGYRVAGEPLGEVVDPLYPGGSFDPLGLAEDPEAF 207

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB22_TOBAC (Chlorophyll a-b binding protein 21, chloroplastic OS=Nicotiana tabacum GN=CAB21 PE=2 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 1.800e-78
Identity = 135/158 (85.44%), Postives = 146/158 (92.41%), Query Frame = 3

Query:   63 FVGKAVNLAPSGNELSNGGRISMRKTGSKSVSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            F G+AV L+PS  E++  GR+SMRKT +K V+S SPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQV+L G+
Sbjct:   13 FAGQAVKLSPSAPEITGNGRVSMRKTVAKPVASSSPWYGPDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVILMGA 170

HSP 2 Score: 82.4185 bits (202), Expect = 2.187e-15
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGAVEGYR+AGGPLGEV DPLYPGGSFDPLGLA+DPEAF
Sbjct:  158 LAIWACQVILMGAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEAF 206

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB2B_SOLLC (Chlorophyll a-b binding protein 1B, chloroplastic OS=Solanum lycopersicum GN=CAB1B PE=3 SV=1)

HSP 1 Score: 291.582 bits (745), Expect = 2.350e-78
Identity = 136/158 (86.08%), Postives = 145/158 (91.77%), Query Frame = 3

Query:   63 FVGKAVNLAPSGNELSNGGRISMRKTGSKSVSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            F G+AV L+PS +E+S  GRI+MRK  +KS  S SPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQVVL G+
Sbjct:   13 FAGQAVKLSPSASEISGNGRITMRKAVAKSAPSSSPWYGPDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGA 170

HSP 2 Score: 82.8037 bits (203), Expect = 1.674e-15
Identity = 40/49 (81.63%), Postives = 42/49 (85.71%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGAVEGYRIAGGPLGEV DPLYPGGSFDPLGLA+DPEAF
Sbjct:  158 LAIWACQVVLMGAVEGYRIAGGPLGEVVDPLYPGGSFDPLGLAEDPEAF 206

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB25_TOBAC (Chlorophyll a-b binding protein 50, chloroplastic OS=Nicotiana tabacum GN=CAB50 PE=2 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 4.009e-78
Identity = 136/160 (85.00%), Postives = 149/160 (93.12%), Query Frame = 3

Query:   63 FVGKAVNLAPSGNELSNGGRISMRKTGSKS--VSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            F GKAV L+PS +E++  G+++MRKT +K+  +SSGSPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQVVL G+
Sbjct:   13 FAGKAVKLSPSSSEITGNGKVTMRKTVTKAKPLSSGSPWYGPDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGA 172

HSP 2 Score: 82.4185 bits (202), Expect = 2.187e-15
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGAVEGYR+AGGPLGEV DPLYPGGSFDPLGLA+DPEAF
Sbjct:  160 LAIWACQVVLMGAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEAF 208

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB21_CUCSA (Chlorophyll a-b binding protein of LHCII type I, chloroplastic (Fragment) OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.990e-77
Identity = 134/156 (85.90%), Postives = 144/156 (92.31%), Query Frame = 3

Query:   69 GKAVNLAPSGNELSNGGRISMRKTGSKSVSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            G+AV L+P+  E+    + +MRKT SKSVSSGSPWYGPDRVKYLGP SGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIH RWAMLGALGCVFPELL+RNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQVVL G+
Sbjct:    5 GQAVKLSPNAPEIQGNAKFTMRKTASKSVSSGSPWYGPDRVKYLGPFSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHSRWAMLGALGCVFPELLSRNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGA 160

HSP 2 Score: 85.5001 bits (210), Expect = 2.583e-16
Identity = 41/49 (83.67%), Postives = 43/49 (87.76%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGAVEGYRIAGGPLGEVTDP+YPGGSFDPLGLADDPEAF
Sbjct:  148 LAIWACQVVLMGAVEGYRIAGGPLGEVTDPIYPGGSFDPLGLADDPEAF 196

[Close]

BLAST of CF417605 vs. ExPASy Swiss-Prot
Match: CB27_TOBAC (Chlorophyll a-b binding protein 7, chloroplastic OS=Nicotiana tabacum GN=CAB7 PE=3 SV=1)

HSP 1 Score: 287.345 bits (734), Expect = 4.433e-77
Identity = 136/160 (85.00%), Postives = 146/160 (91.25%), Query Frame = 3

Query:   63 FVGKAVNLAPSGNELSNGGRISMRKTGSKS--VSSGSPWYGPDRVKYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLXGS 536
            F G AV L+PS +E++  G+  MRKT +K+  VSSGSPWYGPDRVKYLGP SGE PSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG+QIFSEGGLDYLGNPSL+HAQSILAIWACQVVL G+
Sbjct:   13 FAGNAVKLSPSSSEITGNGKAIMRKTVTKAKPVSSGSPWYGPDRVKYLGPFSGEAPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGA 172

HSP 2 Score: 85.5001 bits (210), Expect = 2.583e-16
Identity = 40/49 (81.63%), Postives = 43/49 (87.76%), Query Frame = 1

Query:  511 LARWSX----MGAVEGYRIAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 645
            LA W+     MGA+EGYR+AGGPLGEVTDPLYPGGSFDPLGLADDPEAF
Sbjct:  160 LAIWACQVVLMGAIEGYRVAGGPLGEVTDPLYPGGSFDPLGLADDPEAF 208

The following BLAST results are available for this feature:

BLAST of CF417605 vs. ExPASy Swiss-Prot

BLAST of CF417605 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 70


Match Name	E-value	Identity	Description
CB23_APIGR	1.823e-90	84.62	Chlorophyll a-b binding protein, chloroplastic OS=...	[more]
CB24_TOBAC	3.626e-79	86.25	Chlorophyll a-b binding protein 40, chloroplastic ...	[more]
CB21_TOBAC	4.736e-79	86.25	Chlorophyll a-b binding protein 16, chloroplastic ...	[more]
CB2G_SOLLC	1.055e-78	85.63	Chlorophyll a-b binding protein 3C, chloroplastic ...	[more]
CB25_NICPL	1.055e-78	85.63	Chlorophyll a-b binding protein E, chloroplastic O...	[more]
CB22_TOBAC	1.800e-78	85.44	Chlorophyll a-b binding protein 21, chloroplastic ...	[more]
CB2B_SOLLC	2.350e-78	86.08	Chlorophyll a-b binding protein 1B, chloroplastic ...	[more]
CB25_TOBAC	4.009e-78	85.00	Chlorophyll a-b binding protein 50, chloroplastic ...	[more]
CB21_CUCSA	1.990e-77	85.90	Chlorophyll a-b binding protein of LHCII type I, c...	[more]
CB27_TOBAC	4.433e-77	85.00	Chlorophyll a-b binding protein 7, chloroplastic O...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-535 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-007_B06 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417605 ID=CF417605; Name=CF417605; organism=Citrus sinensis; type=EST; length=646bp
CAGCTTCTGCTTCTCAATTAAATTCCTCAATGGCAACCTCAACAATGGCT
CTCTCCTCATCATTCGTCGGCAAGGCCGTGAACCTCGCCCCTTCAGGCAA
TGAGCTGAGCAATGGTGGCCGCATCTCCATGCGTAAAACCGGGAGCAAGT
CTGTTTCTTCAGGTAGCCCGTGGTACGGCCCGGACAGAGTCAAGTACTTG
GGCCCACTCTCTGGCGAGCCCCCAAGCTACTTGACTGGTGAGTTCCCTGG
CGACTACGGTTGGGACACTGCCGGGCTTTCAGCTGACCCAGAAACCTTTG
CCAAGAACCGTGAACTCGAAGTCATCCACTGCAGATGGGCCATGTTGGGA
GCTCTTGGATGCGTCTTCCCGGAGCTCTTGGCCCGCAACGGAGTCAAGTT
CGGTGAGGCTGTGTGGTTCAAGGCGGGAGCTCAGATCTTCAGCGAGGGCG
GGCTTGACTACTTGGGAAACCCAAGCTTGATCCACGCCCAGAGCATCTTG
GCCATCTGGGCTTGCCAGGTGGTCTTGNATGGGAGCCGTTGAGGGTTACA
GAATTGCTGGAGGGCCGCTCGGTGAAGTGACTGACCCATTGTACCCAGGT
GGCAGCTTTGATCCATTGGGTTTGGCTGATGACCCGGAGGCTTTTG