CF417613 | Citrus Genome Database

Overview


Name	CF417613
Unique Name	CF417613
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	693

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CF417613_ssr299	microsatellite	CF417613_ssr299:299..310.	BLAST: Citrus ESTs to Prunus persica proteins V1
CF417613_ssr318	microsatellite	CF417613_ssr318:318..329.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig12293	contig	Csv1_Contig12293:64..755.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1B2_ARATH (Elongation factor 1-beta 2 OS=Arabidopsis thaliana GN=At5g19510 PE=1 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 1.767e-58
Identity = 92/179 (51.40%), Postives = 110/179 (61.45%), Query Frame = 1

Query:   25 MAITFSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSSRLAPSFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            MA+TFSDLHTE G+KS++++L+GK+YISG+QL+ DD+KVYAAV VKP+D+FPNAS WY+SV+S+LA SF                                 +DLFGD                             VL+DVKPWDDETDMKKLEEAVR VEMPGLFWGAS LVPVGYG
Sbjct:    1 MAVTFSDLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVASQLAKSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEEREAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYG 179

HSP 2 Score: 70.8626 bits (172), Expect = 1.767e-58
Identity = 35/44 (79.55%), Postives = 38/44 (86.36%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKL IM TI DDLVS D+LIE+ LT+EP NEYIQSCDIVAFN
Sbjct:  179 GIKKLTIMFTIVDDLVSPDNLIEDFLTSEPNNEYIQSCDIVAFN 222

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1B1_ARATH (Elongation factor 1-beta 1 OS=Arabidopsis thaliana GN=At5g12110 PE=2 SV=2)

HSP 1 Score: 164.851 bits (416), Expect = 1.339e-55
Identity = 91/183 (49.73%), Postives = 105/183 (57.38%), Query Frame = 1

Query:   25 MAITFSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSSRLAPSF----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            MA+TFSDLHTE GLK+L+++L+GK+YISG+QL+ DD+KVYAAVL  P D FPNAS WYDSV+S LA SF                                     +DLF D                             VLL+VKPWDDETDMKKLEEAVRSV+MPGL WGAS LVPVGYG
Sbjct:    1 MAVTFSDLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASHLAKSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAEEREAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPVGYG 183

HSP 2 Score: 73.1738 bits (178), Expect = 1.339e-55
Identity = 36/44 (81.82%), Postives = 40/44 (90.91%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKL IM+TI DDLVSVD+LIE+HLT+EP NEYIQS DIVAFN
Sbjct:  183 GIKKLTIMMTIVDDLVSVDNLIEDHLTSEPNNEYIQSVDIVAFN 226

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1B_ORYSJ (Elongation factor 1-beta OS=Oryza sativa subsp. japonica GN=Os07g0662500 PE=1 SV=3)

HSP 1 Score: 152.525 bits (384), Expect = 5.486e-54
Identity = 87/180 (48.33%), Postives = 102/180 (56.67%), Query Frame = 1

Query:   25 MAITFSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSSRLAPSF-XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            MA+TF+DLHT  GLK+L+Q+LSGK+Y+SGN ++KDDIKV+AAV  KP   FPNA+ WYD+V++ LA  F                                  LDLFGD                             VLLDVKPWDDETDMKKLEEAVRSV+M GL WGAS LVPVGYG
Sbjct:    1 MAVTFTDLHTADGLKALEQHLSGKTYVSGNAISKDDIKVFAAVPSKPGAEFPNAARWYDTVAAALASRFPGKAVGVNLPGGGAASSAAAAAPAAKDADEDDDDLDLFGD-ETEEDKKAADERAASKASSKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSVQMEGLTWGASKLVPVGYG 179

HSP 2 Score: 80.1073 bits (196), Expect = 5.486e-54
Identity = 38/44 (86.36%), Postives = 41/44 (93.18%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKLQIM+TI DDLVSVDSLIEEHLT EP NE++QSCDIVAFN
Sbjct:  179 GIKKLQIMLTIVDDLVSVDSLIEEHLTEEPINEFVQSCDIVAFN 222

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1B_WHEAT (Elongation factor 1-beta OS=Triticum aestivum PE=1 SV=2)

HSP 1 Score: 145.976 bits (367), Expect = 1.063e-45
Identity = 80/179 (44.69%), Postives = 103/179 (57.54%), Query Frame = 1

Query:   25 MAITFSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSSRLAPSFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            MA+TFSDLHT  GLK+L+ +L+GK+YISG+ +TKDD+KV+AAV +KP+  FPNA+ WYD+V++ ++  F                                 +DLFGD                             VL+D+KPWDDETDMKKLEEAVRSV+M GL WGAS L+PVGYG
Sbjct:    1 MAVTFSDLHTADGLKALEAHLAGKTYISGDGITKDDVKVFAAVPLKPSAEFPNAARWYDTVAAAVSSRF----PGQASGVSASSAPAAAAPAASKDEDDDDDMDLFGD--ETEEDKKAAAEREAAKPAKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSVQMEGLTWGASKLMPVGYG 173

HSP 2 Score: 58.9214 bits (141), Expect = 1.063e-45
Identity = 31/44 (70.45%), Postives = 33/44 (75.00%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKLQIM+TI DDL S    IEE L   P NEY+QSCDIVAFN
Sbjct:  173 GIKKLQIMLTIIDDLASTP--IEEVLCEAPINEYVQSCDIVAFN 214

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1D_PIMBR (Elongation factor 1-delta OS=Pimpinella brachycarpa PE=2 SV=3)

HSP 1 Score: 122.479 bits (306), Expect = 1.151e-44
Identity = 71/181 (39.23%), Postives = 91/181 (50.28%), Query Frame = 1

Query:   25 MAITFSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSS--RLAPSFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            MA+TF DL +E+GL+ LD+YL  +SYISG Q +KDD+ V+AA+   P+  + N S WY+ V +  R++                                    +DLFG+                             VLLDVKPWDDETDM KLEEAVRS++M GL WGAS LV VGYG
Sbjct:    1 MAVTFYDLSSEAGLEKLDEYLLSRSYISGYQASKDDLAVHAALAKPPSSKYVNVSRWYNHVEALLRISGVSAEGCGVTVEGSSVATPPVADTKASAAEDDDDDDVDLFGEETEEEKKASEERAAAVKASGKKKESGKSSVLLDVKPWDDETDMTKLEEAVRSIKMDGLLWGASKLVAVGYG 181

HSP 2 Score: 78.9518 bits (193), Expect = 1.151e-44
Identity = 37/44 (84.09%), Postives = 41/44 (93.18%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKLQIM+TI DDLVSVD L+E++LTAEP NEYIQSCDIVAFN
Sbjct:  181 GIKKLQIMLTIVDDLVSVDDLVEDYLTAEPANEYIQSCDIVAFN 224

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1D2_ORYSJ (Elongation factor 1-delta 2 OS=Oryza sativa subsp. japonica GN=Os03g0406200 PE=2 SV=3)

HSP 1 Score: 122.479 bits (306), Expect = 6.112e-43
Identity = 72/181 (39.78%), Postives = 93/181 (51.38%), Query Frame = 1

Query:   25 MAITFSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSSRLAPS--FXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            MAIT S++++E+GL+ LD+YL  +SYISG Q +KDD+ V+ ++   PA S+ N + WYD +S+ L  S                                    +DLFG+                             VLLDVKPWDDETDM KLEEAVR+V+M GL WGAS LVPVGYG
Sbjct:    1 MAITLSNVNSEAGLQKLDEYLLTRSYISGYQASKDDMTVFTSLPSAPAASYVNVTRWYDHISALLRSSGVTAEGEGVKVESTACSVSPTADQKAPAADEEDDDDVDLFGEETEEEKKAAEERAAAVKASGKKKESGKSSVLLDVKPWDDETDMAKLEEAVRNVKMEGLLWGASKLVPVGYG 181

HSP 2 Score: 73.1738 bits (178), Expect = 6.112e-43
Identity = 35/44 (79.55%), Postives = 39/44 (88.64%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKLQIM+TI DDLVSVDSLIE++   EP NE+IQSCDIVAFN
Sbjct:  181 GIKKLQIMMTIVDDLVSVDSLIEDYFYTEPANEFIQSCDIVAFN 224

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1D1_ARATH (Elongation factor 1-delta 1 OS=Arabidopsis thaliana GN=At1g30230 PE=2 SV=2)

HSP 1 Score: 116.701 bits (291), Expect = 1.464e-41
Identity = 67/183 (36.61%), Postives = 90/183 (49.18%), Query Frame = 1

Query:   37 FSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSSRLAPS--------FXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            F +L++++GLK LD++L  +SYI+G Q +KDDI V+AA+   P   + NAS WY+ + + L  S                                          +DLFG+                             VL+D+KPWDDETDMKKLEEAV+S++M GLFWGAS LVPVGYG
Sbjct:    4 FPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDALLRISGVSAEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGEETEEEKKAAEERAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWGASKLVPVGYG 186

HSP 2 Score: 74.3294 bits (181), Expect = 1.464e-41
Identity = 34/44 (77.27%), Postives = 39/44 (88.64%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKLQI+ TI DDLVS+D++IEE LT EP NEY+QSCDIVAFN
Sbjct:  186 GIKKLQILCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIVAFN 229

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1D1_ORYSJ (Elongation factor 1-delta 1 OS=Oryza sativa subsp. japonica GN=Os07g0614500 PE=2 SV=3)

HSP 1 Score: 116.316 bits (290), Expect = 1.464e-41
Identity = 68/184 (36.96%), Postives = 90/184 (48.91%), Query Frame = 1

Query:   25 MAITFSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSSRLAPSFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----LDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            MA++F+++ +E+GLK LD+YL  +SYISG Q + DD+ VY+A    P+ S+ N + W+  + + L  S                                       +DLFG+                             VLLDVKPWDDETDM KLEEAVR+V+M GL WGAS LVPVGYG
Sbjct:    1 MAVSFTNVSSEAGLKKLDEYLLTRSYISGYQASNDDLAVYSAFSTAPSSSYTNVARWFTHIDALLRLSGVTADGQGVKVESTAVPSASTPDVADAKAPAADDDDDDDVDLFGEETEEEKKAAEERAAAVKASGKKKESGKSSVLLDVKPWDDETDMTKLEEAVRNVKMEGLLWGASKLVPVGYG 184

HSP 2 Score: 74.7146 bits (182), Expect = 1.464e-41
Identity = 36/44 (81.82%), Postives = 39/44 (88.64%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKLQIM+TI DDLVSVDSLIE++   EP NEYIQSCDIVAFN
Sbjct:  184 GIKKLQIMMTIVDDLVSVDSLIEDYFYTEPANEYIQSCDIVAFN 227

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1D2_ARATH (Elongation factor 1-delta 2 OS=Arabidopsis thaliana GN=At2g18110 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 2.065e-40
Identity = 67/183 (36.61%), Postives = 87/183 (47.54%), Query Frame = 1

Query:   37 FSDLHTESGLKSLDQYLSGKSYISGNQLTKDDIKVYAAVLVKPADSFPNASSWYDSVSSRLAPS--------FXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLFGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            F +L++ SGLK LD++L  +SYI+G Q +KDDI V+ A+   P   F N S W++ + + L  S                                          +DLFG+                             VL+D+KPWDDETDMKKLEEAVRS++M GLFWGAS LVPVGYG
Sbjct:    4 FPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDALLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGEETEEEKKAAEERAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWGASKLVPVGYG 186

HSP 2 Score: 73.559 bits (179), Expect = 2.065e-40
Identity = 34/44 (77.27%), Postives = 38/44 (86.36%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKL IM TI DDLVS+D++IEE LT EP NEY+QSCDIVAFN
Sbjct:  186 GIKKLHIMCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIVAFN 229

[Close]

BLAST of CF417613 vs. ExPASy Swiss-Prot
Match: EF1D_BETVU (Elongation factor 1-delta OS=Beta vulgaris PE=2 SV=3)

HSP 1 Score: 74.7146 bits (182), Expect = 4.772e-29
Identity = 35/44 (79.55%), Postives = 40/44 (90.91%), Query Frame = 2

Query:  560 GIKKLQIMITIXDDLVSVDSLIEEHLTAEPCNEYIQSCDIVAFN 691
            GIKKL IM+TI DDLVSVD+LIE++LT EP NEY+QSCDIVAFN
Sbjct:  186 GIKKLTIMMTIVDDLVSVDNLIEDYLTVEPINEYVQSCDIVAFN 229

HSP 2 Score: 74.3294 bits (181), Expect = 4.772e-29
Identity = 36/42 (85.71%), Postives = 37/42 (88.10%), Query Frame = 1

Query:  436 VLLDVKPWDDETDMKKLEEAVRSVEMPGLFWGASXLVPVGYG 561
            VLLDVKPWDDETDMKKLEEAVRSV+  GL  GAS LVPVGYG
Sbjct:  145 VLLDVKPWDDETDMKKLEEAVRSVQQEGLTLGASKLVPVGYG 186

The following BLAST results are available for this feature:

BLAST of CF417613 vs. ExPASy Swiss-Prot

BLAST of CF417613 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34


Match Name	E-value	Identity	Description
EF1B2_ARATH	1.767e-58	51.40	Elongation factor 1-beta 2 OS=Arabidopsis thaliana...	[more]
EF1B1_ARATH	1.339e-55	49.73	Elongation factor 1-beta 1 OS=Arabidopsis thaliana...	[more]
EF1B_ORYSJ	5.486e-54	48.33	Elongation factor 1-beta OS=Oryza sativa subsp. ja...	[more]
EF1B_WHEAT	1.063e-45	44.69	Elongation factor 1-beta OS=Triticum aestivum PE=1...	[more]
EF1D_PIMBR	1.151e-44	39.23	Elongation factor 1-delta OS=Pimpinella brachycarp...	[more]
EF1D2_ORYSJ	6.112e-43	39.78	Elongation factor 1-delta 2 OS=Oryza sativa subsp....	[more]
EF1D1_ARATH	1.464e-41	36.61	Elongation factor 1-delta 1 OS=Arabidopsis thalian...	[more]
EF1D1_ORYSJ	1.464e-41	36.96	Elongation factor 1-delta 1 OS=Oryza sativa subsp....	[more]
EF1D2_ARATH	2.065e-40	36.61	Elongation factor 1-delta 2 OS=Arabidopsis thalian...	[more]
EF1D_BETVU	4.772e-29	79.55	Elongation factor 1-delta OS=Beta vulgaris PE=2 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_115000-543 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-007_C03 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417613 ID=CF417613; Name=CF417613; organism=Citrus sinensis; type=EST; length=693bp
CTCTGCCTTGATTTGAAGACCAAAATGGCCATCACTTTTTCGGATCTCCA
CACAGAGTCAGGCCTTAAGTCTCTCGATCAGTATCTTTCTGGGAAATCTT
ATATCTCCGGTAATCAACTGACAAAAGATGACATCAAGGTCTACGCTGCC
GTTTTGGTGAAACCTGCTGATTCTTTCCCCAACGCCAGCAGCTGGTACGA
CAGCGTTTCTTCACGTCTCGCCCCAAGCTTCCCTGGCAAAGCCGTTGGTG
TGANAGTTGATGGCAAAGGTGCTCCAGTTGACGCCGCCGCAGCCAAGCCT
GCTGCTGCTGGAGGTGGTGATGATGATGATTTGGATCTCTTTGGAGATGA
GACTGAGGAGGATAAGAAAGCAGCAGAGGAGAGGGAGGCTGCTAAAAAGC
AATCATCCAAGAAGAAAGAGAGTGGAAAATCATCGGTACTTTTGGATGTA
AAGCCTTGGGATGACGAGACAGACATGAAAAAGCTAGAAGAGGCAGTTAG
GAGTGTTGAGATGCCGGGTCTCTTTTGGGGAGCATCAANATTGGTCCCAG
TCGGTTATGGGGATTAAGAAGCTGCAAATCATGATTACNATCGNTGACGA
TCTTGTTTCTGTGGATTCACTGATAGAGGAACACCTCACAGCTGAGCCCT
GCAATGAGTATATTCAGAGCTGCGACATCGTCGCCTTCAATAA