CF503954

Overview
NameCF503954
Unique NameCF503954
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length631
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF503954_ssr342 microsatellite CF503954_ssr342:342..353. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig2047 contig Csv1_Contig2047:104..733. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL212_ARATH (60S ribosomal protein L21-2 OS=Arabidopsis thaliana GN=RPL21E PE=2 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 5.929e-87
Identity = 148/164 (90.24%), Postives = 159/164 (96.95%), Query Frame = 1
Query:   19 MPAGHGLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMVEGATLETVTPIPYDVVNDLKGGY 510
            MPAGHG+R+RTRDLFARPFRKKGYIPLSTYLRT+K+GDYVDVKVNGA+HKGMPHKFYHGRTGR+WNVTKRA+GVE+NKQ+GNRII+KRIHVRVEHVQ SRC EEFKLRKK+ND LKADAKARGE ISTKRQPKGPKPGFMVEG TLETVTPIPYDVVNDLKGGY
Sbjct:    1 MPAGHGVRARTRDLFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIWNVTKRAVGVEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKQNDVLKADAKARGETISTKRQPKGPKPGFMVEGMTLETVTPIPYDVVNDLKGGY 164          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL211_ARATH (60S ribosomal protein L21-1 OS=Arabidopsis thaliana GN=RPL21A PE=2 SV=2)

HSP 1 Score: 319.316 bits (817), Expect = 1.011e-86
Identity = 148/164 (90.24%), Postives = 158/164 (96.34%), Query Frame = 1
Query:   19 MPAGHGLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMVEGATLETVTPIPYDVVNDLKGGY 510
            MPAGHG+R+RTRDLFARPFRKKGYIPLSTYLRT+K+GDYVDVKVNGA+HKGMPHKFYHGRTGR+WNVTKRA+GVE+NKQ+GNRII+KRIHVRVEHVQ SRC EEFKLRKKKNDELKA AKA GE ISTKRQPKGPKPGFMVEG TLETVTPIPYDVVNDLKGGY
Sbjct:    1 MPAGHGVRARTRDLFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIWNVTKRAVGVEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKKNDELKAAAKANGETISTKRQPKGPKPGFMVEGMTLETVTPIPYDVVNDLKGGY 164          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL21_CYAPA (60S ribosomal protein L21 OS=Cyanophora paradoxa GN=RPL21 PE=2 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 2.133e-44
Identity = 94/158 (59.49%), Postives = 115/158 (72.78%), Query Frame = 1
Query:   19 MPAGHGLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAK-ARGEVISTKRQPKGPKPGFMV--EGATLETVTPIPYD 483
            MP  +G R+RTR LFAR F+  G + LSTYL  +KIG+YVDV  N AVHKGMP K+YHGRTG VWNVTKRAIGVEINK+V +RIIKKRIHVR++HV+ S C + F  R K+ND++K DA  A+  V STKR P  PK G ++  +G   ET   IPY+
Sbjct:    1 MPHSYGYRARTRALFARAFKTNGPVHLSTYLTNFKIGEYVDVVGNAAVHKGMPFKYYHGRTGIVWNVTKRAIGVEINKRVRHRIIKKRIHVRIDHVKKSSCRDSFLKRVKENDKIKHDAHVAKLPVPSTKRLPVLPKAGAIIKSKGQVPETCHAIPYE 158          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL21A_YEAST (60S ribosomal protein L21-A OS=Saccharomyces cerevisiae GN=RPL21A PE=1 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 1.806e-43
Identity = 83/157 (52.87%), Postives = 113/157 (71.97%), Query Frame = 1
Query:   19 MPAGHGLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMV--EGATLETVTPIPYD 483
            M   HG RSRTR +F R FRK G + LSTYL+ YK+GD VD+K NG++ KGMPHKFY G+TG V+NVTK ++GV INK VGNR ++KR+++RVEH++ S+C +EF  R K N   +A+AKA+G  +  KRQP  P+   +V  EG   +T+ P+PY+
Sbjct:    1 MGKSHGYRSRTRYMFQRDFRKHGAVHLSTYLKVYKVGDIVDIKANGSIQKGMPHKFYQGKTGVVYNVTKSSVGVIINKMVGNRYLEKRLNLRVEHIKHSKCRQEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQTLAPVPYE 157          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL21B_YEAST (60S ribosomal protein L21-B OS=Saccharomyces cerevisiae GN=RPL21B PE=1 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 4.023e-43
Identity = 82/157 (52.23%), Postives = 113/157 (71.97%), Query Frame = 1
Query:   19 MPAGHGLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMV--EGATLETVTPIPYD 483
            M   HG RSRTR +F R FRK G + +STYL+ YK+GD VD+K NG++ KGMPHKFY G+TG V+NVTK ++GV INK VGNR ++KR+++RVEH++ S+C +EF  R K N   +A+AKA+G  +  KRQP  P+   +V  EG   +T+ P+PY+
Sbjct:    1 MGKSHGYRSRTRYMFQRDFRKHGAVHMSTYLKIYKVGDIVDIKANGSIQKGMPHKFYQGKTGVVYNVTKSSVGVIINKMVGNRYLEKRLNLRVEHIKHSKCRQEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQTLAPVPYE 157          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL21_DICDI (60S ribosomal protein L21 OS=Dictyostelium discoideum GN=rpl21 PE=3 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 8.963e-43
Identity = 84/159 (52.83%), Postives = 108/159 (67.92%), Query Frame = 1
Query:   19 MPAGHGLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMVEGATLE--TVTPIPYDVV 489
            MP   GLR+RTR LF+R FR  G +  +T+LRT+++GDYVD+K  G VHKGMPHKFYHGRTGRVWNVT RA+GV INK+VG RII K+IHVR EHV+PS        R ++N +   +AK  G+ +  +R P  PK GF V     E   V P+ Y+++
Sbjct:    1 MPHSFGLRARTRYLFSRGFRNHGLLQTTTFLRTFRLGDYVDIKATGNVHKGMPHKFYHGRTGRVWNVTPRAVGVIINKRVGPRIIAKKIHVRTEHVKPSNSMAAHLKRIQENKKAVVEAKKAGKWVDVRRNPAAPKDGFFVNPRNTEFVEVKPVKYELL 159          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL21B_SCHPO (60S ribosomal protein L21-B OS=Schizosaccharomyces pombe GN=rpl21b PE=2 SV=2)

HSP 1 Score: 169.859 bits (429), Expect = 9.910e-42
Identity = 82/157 (52.23%), Postives = 112/157 (71.34%), Query Frame = 1
Query:   19 MPAGHGLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMVEGATLETVT--PIPYD 483
            MP  +G+R+RTR  F R FR+ G I LSTYL+TYK+GD VD+KVNGAV KGMPHK+YHG+TG V+NVT+ ++GV I K VGNR ++KR++VR+EHV+ S+C ++F  R K N+  + +AKA+G+ +  +RQP  P     V     E VT  P+ YD
Sbjct:    1 MPHSYGIRARTRYTFQRGFREHGQIRLSTYLKTYKVGDIVDIKVNGAVQKGMPHKYYHGKTGVVYNVTQSSVGVLIYKVVGNRYMEKRVNVRIEHVKHSKCRQDFLDRVKANEAKRKEAKAQGKTVQLRRQPAPPATAHFVSTENNEPVTLHPVAYD 157          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL21A_SCHPO (60S ribosomal protein L21-A OS=Schizosaccharomyces pombe GN=rpl21a PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 9.910e-42
Identity = 82/157 (52.23%), Postives = 112/157 (71.34%), Query Frame = 1
Query:   19 MPAGHGLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMVEGATLETVT--PIPYD 483
            MP  +G+R+RTR  F R FR+ G I LSTYL+TYK+GD VD+KVNGAV KGMPHK+YHG+TG V+NVT+ ++GV I K VGNR ++KR++VR+EHV+ S+C ++F  R K N+  + +AKA+G+ +  +RQP  P     V     E VT  P+ YD
Sbjct:    1 MPHSYGIRARTRYTFQRGFREHGQIRLSTYLKTYKVGDIVDIKVNGAVQKGMPHKYYHGKTGVVYNVTQSSVGVLIYKVVGNRYMEKRVNVRIEHVKHSKCRQDFLDRVKANEAKRKEAKAQGKTVQLRRQPAPPAKAHFVSTENNEPVTLHPVAYD 157          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL21_HUMAN (60S ribosomal protein L21 OS=Homo sapiens GN=RPL21 PE=1 SV=2)

HSP 1 Score: 161.384 bits (407), Expect = 3.525e-39
Identity = 77/152 (50.66%), Postives = 105/152 (69.08%), Query Frame = 1
Query:   34 GLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMV--EGATLETVTPIPYD 483
            G R  TR +F+RPFRK G +PL+TY+R YK GD VD+K  G V KGMPHK YHG+TGRV+NVT+ A+G+ +NKQV  +I+ KRI+VR+EH++ S+  + F  R K+ND+ K +AK +G  +  KRQP  P+    V   G   E + PIPY+
Sbjct:    6 GKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYE 157          
BLAST of CF503954 vs. ExPASy Swiss-Prot
Match: RL21_CHILA (60S ribosomal protein L21 OS=Chinchilla lanigera GN=RPL21 PE=2 SV=3)

HSP 1 Score: 160.229 bits (404), Expect = 7.852e-39
Identity = 76/152 (50.00%), Postives = 105/152 (69.08%), Query Frame = 1
Query:   34 GLRSRTRDLFARPFRKKGYIPLSTYLRTYKIGDYVDVKVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEINKQVGNRIIKKRIHVRVEHVQPSRCTEEFKLRKKKNDELKADAKARGEVISTKRQPKGPKPGFMV--EGATLETVTPIPYD 483
            G R  TR +F+RP+RK G +PL+TY+R YK GD VD+K  G V KGMPHK YHG+TGRV+NVT+ A+G+ +NKQV  +I+ KRI+VR+EH++ S+  + F  R K+ND+ K +AK +G  +  KRQP  P+    V   G   E + PIPY+
Sbjct:    6 GKRRGTRYMFSRPYRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYE 157          
The following BLAST results are available for this feature:
BLAST of CF503954 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 23
Match NameE-valueIdentityDescription
RL212_ARATH5.929e-8790.2460S ribosomal protein L21-2 OS=Arabidopsis thalian... [more]
RL211_ARATH1.011e-8690.2460S ribosomal protein L21-1 OS=Arabidopsis thalian... [more]
RL21_CYAPA2.133e-4459.4960S ribosomal protein L21 OS=Cyanophora paradoxa G... [more]
RL21A_YEAST1.806e-4352.8760S ribosomal protein L21-A OS=Saccharomyces cerev... [more]
RL21B_YEAST4.023e-4352.2360S ribosomal protein L21-B OS=Saccharomyces cerev... [more]
RL21_DICDI8.963e-4352.8360S ribosomal protein L21 OS=Dictyostelium discoid... [more]
RL21B_SCHPO9.910e-4252.2360S ribosomal protein L21-B OS=Schizosaccharomyces... [more]
RL21A_SCHPO9.910e-4252.2360S ribosomal protein L21-A OS=Schizosaccharomyces... [more]
RL21_HUMAN3.525e-3950.6660S ribosomal protein L21 OS=Homo sapiens GN=RPL21... [more]
RL21_CHILA7.852e-3950.0060S ribosomal protein L21 OS=Chinchilla lanigera G... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-442 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-11_F08 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF503954 ID=CF503954; Name=CF503954; organism=Citrus sinensis; type=EST; length=631bp
GGAGCAGAGTACTCAAACATGCCGGCAGGTCACGGTTTGAGGTCCCGAAC
AAGGGATTTGTTCGCACGCCCCTTCAGAAAGAAGGGTTACATCCCCTTGT
CCACTTACCTTAGGACTTACAAAATCGGCGACTACGTTGACGTCAAAGTG
AACGGCGCCGTTCACAAAGGTATGCCCCACAAGTTCTACCATGGGCGTAC
AGGCCGTGTCTGGAATGTCACCAAGCGCGCCATCGGCGTGGAGATTAACA
AGCAGGTTGGTAACAGGATCATCAAGAAGAGGATTCATGTTCGTGTGGAG
CATGTTCAACCTTCAAGGTGTACCGAGGAGTTCAAGCTGAGGAAGAAGAA
GAATGATGAGCTCAAGGCAGATGCTAAGGCAAGAGGTGAGGTCATCAGTA
CAAAGAGGCAGCCAAAGGGCCCTAAACCAGGTTTCATGGTGGAAGGTGCA
ACATTGGAAACTGTCACTCCTATTCCTTATGATGTCGTCAATGATCTTAA
GGGTGGTTACTAGGTTATCTTTTTGGTATTGGTGCTGTTTTGTTACTTGT
TGCAGACTTTTCTGGACATGGTCTTATTTGGATGTATTTTCATCCTTATT
AGTTTTGTTTTTGGAGAGGAACTGCAATTGC
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