CF503972

Overview
NameCF503972
Unique NameCF503972
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length520
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12923 contig Csv1_Contig12923:8..524. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 1.261e-39
Identity = 67/105 (63.81%), Postives = 81/105 (77.14%), Query Frame = 2 
Query:  164 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPC 472
            +L  IISR  FDD+L++RND  CPARGFYTYDAFI AA++FPGFG +G+   RK+EIAAFF QT HETTGGW  AP G Y+WGYCF  E +P S+YC P+  +PC
Sbjct:   70 DLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGDTATRKKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSAEWPC 174

HSP 2 Score: 31.187 bits (69), Expect = 1.261e-39
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  483 YYGRGPIQLSWN 518
            YYGRGP+QLSWN
Sbjct:  180 YYGRGPMQLSWN 191
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHIT_CARPA (Endochitinase OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 1.650e-39
Identity = 65/102 (63.73%), Postives = 80/102 (78.43%), Query Frame = 2 
Query:  173 KIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYC--DPNYPC 472
            KIISR MFD +L++RN+  CPA+GFYTYDAF+ AA++FP FG +G+  +RKRE+AAF  QT HETTGGWP AP G YAWGYCF+ E +P S+YC   P YPC
Sbjct:    4 KIISRSMFDQMLKHRNNPACPAKGFYTYDAFLAAAKSFPSFGTTGSTDVRKRELAAFLGQTSHETTGGWPSAPDGPYAWGYCFLKERNPSSNYCAPSPRYPC 105

HSP 2 Score: 31.5722 bits (70), Expect = 1.650e-39
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  483 YYGRGPIQLSWN 518
            YYGRGP+QLSWN
Sbjct:  111 YYGRGPLQLSWN 122
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHIB_ARATH (Basic endochitinase B OS=Arabidopsis thaliana GN=CHI-B PE=1 SV=3)

HSP 1 Score: 150.214 bits (378), Expect = 4.733e-39
Identity = 65/105 (61.90%), Postives = 81/105 (77.14%), Query Frame = 2 
Query:  164 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPC 472
            +L  IIS   FDD+L++RND  CPARGFYTY+AFI AA++FPGFG +G+   RK+E+AAFF QT HETTGGW  AP G Y+WGYCF  E +P SDYC+P+  +PC
Sbjct:   85 DLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPC 189

HSP 2 Score: 31.9574 bits (71), Expect = 4.733e-39
Identity = 11/13 (84.62%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  480 KYYGRGPIQLSWN 518
            +YYGRGP+QLSWN
Sbjct:  194 RYYGRGPMQLSWN 206
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHI2_ORYSJ (Chitinase 2 OS=Oryza sativa subsp. japonica GN=Cht2 PE=1 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.014e-38
Identity = 64/104 (61.54%), Postives = 79/104 (75.96%), Query Frame = 2 
Query:  167 LGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPC 472
            +G I+ R++F+ LL +RND  CPARGFYTY+AF+ AA AFP FG +GN   RKRE+AAF  QT HETTGGWP AP G ++WGYCF  E +PPSDYC P+  +PC
Sbjct:   89 VGSIVPRDLFERLLLHRNDGACPARGFYTYEAFLAAAAAFPAFGGTGNTETRKREVAAFLGQTSHETTGGWPTAPDGPFSWGYCFKQEQNPPSDYCQPSPEWPC 192

HSP 2 Score: 30.4166 bits (67), Expect = 3.014e-38
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  480 KYYGRGPIQLSWN 518
            KYYGRGPIQLS+N
Sbjct:  197 KYYGRGPIQLSFN 209
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHIB_VITVI (Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 3.018e-38
Identity = 64/105 (60.95%), Postives = 82/105 (78.10%), Query Frame = 2 
Query:  164 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPC 472
            ++G++I+R MF+D+L++RN+  CP +GFYTYDAFI AA+AFPGFG +G+ T RKREIAAF AQT HETTGGW  AP G YAWGYC++ E   P  YC P+  +PC
Sbjct:   64 DIGQLITRSMFNDMLKHRNEGSCPGKGFYTYDAFIAAAKAFPGFGTTGDTTTRKREIAAFLAQTSHETTGGWASAPDGPYAWGYCYLREQGSPGAYCVPSAQWPC 168

HSP 2 Score: 30.0314 bits (66), Expect = 3.018e-38
Identity = 11/13 (84.62%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  480 KYYGRGPIQLSWN 518
            KYYGRGPIQ+S+N
Sbjct:  173 KYYGRGPIQISYN 185
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHI6_POPTR (Acidic endochitinase WIN6 OS=Populus trichocarpa GN=WIN6 PE=2 SV=2)

HSP 1 Score: 147.132 bits (370), Expect = 1.474e-37
Identity = 67/104 (64.42%), Postives = 79/104 (75.96%), Query Frame = 2 
Query:  167 LGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDP--NYPC 472
            L  II +  FD LL++RND RCPA GFYTY+AFI AA+ FP FGN+G++ MRKREIAAF  QT HETTGGWPDAP G YAWGYC++ E++    YCDP  NY C
Sbjct:   89 LSDIIPKSKFDALLKFRNDARCPAAGFYTYNAFISAAKEFPDFGNTGDDLMRKREIAAFLGQTSHETTGGWPDAPCGPYAWGYCYLKEIN-CQPYCDPSSNYQC 191

HSP 2 Score: 30.0314 bits (66), Expect = 1.474e-37
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  471 VEGKYY-GRGPIQLSWN 518
            V GK Y GRGPIQLSWN
Sbjct:  192 VAGKQYCGRGPIQLSWN 208
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 150.214 bits (378), Expect = 1.474e-37
Identity = 66/105 (62.86%), Postives = 80/105 (76.19%), Query Frame = 2 
Query:  164 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPC 472
            +LG IIS  MFD +L++RND  C  +GFY+Y+AFI AA++FPGFG SG+ T RKREIAAFFAQT HETTGGW  AP G YAWGYC++ E   P DYC P+  +PC
Sbjct:   79 DLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPC 183

HSP 2 Score: 26.9498 bits (58), Expect = 1.474e-37
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  480 KYYGRGPIQLSWN 518
            KY+GRGPIQ+S N
Sbjct:  188 KYFGRGPIQISHN 200
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.475e-37
Identity = 66/105 (62.86%), Postives = 80/105 (76.19%), Query Frame = 2 
Query:  164 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPC 472
            +LG IIS  MFD +L++RND  C  +GFY+Y+AFI AA++FPGFG SG+ T RKREIAAFFAQT HETTGGW  AP G YAWGYC++ E   P DYC P+  +PC
Sbjct:   74 DLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPC 178

HSP 2 Score: 26.9498 bits (58), Expect = 1.475e-37
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  480 KYYGRGPIQLSWN 518
            KY+GRGPIQ+S N
Sbjct:  183 KYFGRGPIQISHN 195
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHIT_PHAVU (Endochitinase OS=Phaseolus vulgaris PE=1 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 3.260e-37
Identity = 65/105 (61.90%), Postives = 78/105 (74.29%), Query Frame = 2 
Query:  164 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCD--PNYPC 472
            +L  +ISR  FD +L++RND  CPA+GFYTYDAFI AA+A+P FGN+G+   RKREIAAF  QT HETTGGW  AP G YAWGYCF+ E + PS YC   P +PC
Sbjct:   75 DLSALISRSTFDQMLKHRNDGACPAKGFYTYDAFIAAAKAYPSFGNTGDTATRKREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVRERN-PSTYCSATPQFPC 178

HSP 2 Score: 31.9574 bits (71), Expect = 3.260e-37
Identity = 11/13 (84.62%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  480 KYYGRGPIQLSWN 518
            +YYGRGPIQ+SWN
Sbjct:  183 QYYGRGPIQISWN 195
[Close]
BLAST of CF503972 vs. ExPASy Swiss-Prot
Match: CHI5_PHAVU (Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 5.532e-37
Identity = 65/105 (61.90%), Postives = 78/105 (74.29%), Query Frame = 2 
Query:  164 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCD--PNYPC 472
            +L  +ISR  FD +L++RND  CPA+GFYTYDAFI AA+A+P FGN+G+   RKREIAAF  QT HETTGGW  AP G YAWGYCF+ E + PS YC   P +PC
Sbjct:   74 DLSALISRSTFDQVLKHRNDGACPAKGFYTYDAFIAAAKAYPSFGNTGDTATRKREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVRERN-PSAYCSATPQFPC 177

HSP 2 Score: 31.9574 bits (71), Expect = 5.532e-37
Identity = 11/13 (84.62%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  480 KYYGRGPIQLSWN 518
            +YYGRGPIQ+SWN
Sbjct:  182 QYYGRGPIQISWN 194
The following BLAST results are available for this feature:
BLAST of CF503972 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55
Match NameE-valueIdentityDescription
CHI2_BRANA1.261e-3963.81Endochitinase CH25 OS=Brassica napus PE=2 SV=1[more]
CHIT_CARPA1.650e-3963.73Endochitinase OS=Carica papaya PE=1 SV=1[more]
CHIB_ARATH4.733e-3961.90Basic endochitinase B OS=Arabidopsis thaliana GN=C... [more]
CHI2_ORYSJ3.014e-3861.54Chitinase 2 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHIB_VITVI3.018e-3860.95Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE... [more]
CHI6_POPTR1.474e-3764.42Acidic endochitinase WIN6 OS=Populus trichocarpa G... [more]
CHI1_TOBAC1.474e-3762.86Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1... [more]
CHI2_TOBAC1.475e-3762.86Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1... [more]
CHIT_PHAVU3.260e-3761.90Endochitinase OS=Phaseolus vulgaris PE=1 SV=1[more]
CHI5_PHAVU5.532e-3761.90Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-460 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-11_H03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF503972 ID=CF503972; Name=CF503972; organism=Citrus sinensis; type=EST; length=520bp
GGATAATTGATATCATAACTGTTGCTTTCAAAATGAGGCTTATTGGGTCC
TTACTAATTTTCTCTCTAGTTTTATCTTTTGTACTAGGAGGCTCAGCACA
GAACTGTGGAAGCCGGCGTTGTGTACCGGTGGACGTGATACCGGCCATGG
GACGCATGGCGGTGAACTGGGCAAGATCATCTCAAGGGAGATGTTTGATG
ACTTGCTTGAGTATAGAAATGATGAACGATGCCCTGCCAGAGGCTTCTAC
ACTTATGATGCTTTTATAGAGGCAGCCCAAGCTTTTCCAGGCTTTGGTAA
CTCTGGAAATGAAACCATGCGTAAAAGAGAAATCGCTGCCTTTTTTGCCC
AAACTGGCCATGAAACTACTGGGGGATGGCCCGATGCACCTGGTGGCGAA
TATGCTTGGGGATATTGCTTTATAAGCGAAGTTAGCCCTCCGTCCGACTA
CTGTGATCCTAACTATCCATGTCGAGGGGAAGTACTATGGCCGAGGTCCA
ATTCAACTCTCTTGGAATTA
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