CF504018

Overview
NameCF504018
Unique NameCF504018
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length610
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF504018_ssr21 microsatellite CF504018_ssr21:21..32. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10199 contig Csv1_Contig10199:400..1011. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504018 vs. ExPASy Swiss-Prot
Match: WRK17_ARATH (Probable WRKY transcription factor 17 OS=Arabidopsis thaliana GN=WRKY17 PE=1 SV=2)

HSP 1 Score: 110.153 bits (274), Expect = 6.278e-43
Identity = 64/124 (51.61%), Postives = 76/124 (61.29%), Query Frame = 3 
Query:  111 SYVQSQPHSLTLDFTKPSLFSGNVKSTEL-EXXXXXXXXXXXXXXXXXAITGDGSVSNGKQGGSSIFLAPQ--APAVSAGKPPLAAQPYKKRCQDHKDHSDDLSGKFSGSTSGNNKCHCSKRRK 473
            S+VQ+   S+TLDFT+PS+F    KS+E+ E                 AITGDGSVS G    SSIFLAP    P  S+GKPPL+  PY+KRC +H DHS+  SGK SG  SGN KCHC K RK
Sbjct:  106 SFVQANQQSVTLDFTRPSVFGAKTKSSEVVEFAKESFSVSSNSSFMSSAITGDGSVSKG----SSIFLAPAPAVPVTSSGKPPLSGLPYRKRCFEH-DHSEGFSGKISG--SGNGKCHCKKSRK 222

HSP 2 Score: 75.485 bits (184), Expect = 6.278e-43
Identity = 31/32 (96.88%), Postives = 32/32 (100.00%), Query Frame = 1 
Query:  514 KIADIPPDEYSWRKYGQKPIKGSPYPRGYYKC 609
            KIADIPPDEYSWRKYGQKPIKGSP+PRGYYKC
Sbjct:  237 KIADIPPDEYSWRKYGQKPIKGSPHPRGYYKC 268

HSP 3 Score: 28.8758 bits (63), Expect = 6.278e-43
Identity = 10/17 (58.82%), Postives = 17/17 (100.00%), Query Frame = 2 
Query:  467 KENRVKKTIRVPAISSK 517
            ++NR+K+T+RVPA+S+K
Sbjct:  221 RKNRMKRTVRVPAVSAK 237
[Close]
BLAST of CF504018 vs. ExPASy Swiss-Prot
Match: WRK11_ARATH (Probable WRKY transcription factor 11 OS=Arabidopsis thaliana GN=WRKY11 PE=1 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 3.035e-42
Identity = 62/117 (52.99%), Postives = 70/117 (59.83%), Query Frame = 3 
Query:  129 PHSLTLDFTKPSLFSGNVKSTELEXXXXXXXXXXXXXXXXXAITGDGSVSNGKQGGSSIFLA--PQAPAVSAGKPPLAAQPYKKRCQDHKDHSDDLSGKFSGSTSGNNKCHCSKRRK 473
            P S+TLDF+KPS+F    KS ELE                 AITGDGSVSNGK     IFLA  P  P  S+GKPPLA  PY+KRC +H +HS+  SGK SGS  G  KCHC K RK
Sbjct:  117 PSSVTLDFSKPSIFGTKAKSAELEFSKENFSVSLNSSFMSSAITGDGSVSNGK-----IFLASAPLQPVNSSGKPPLAGHPYRKRCLEH-EHSESFSGKVSGSAYG--KCHCKKSRK 225

HSP 2 Score: 75.485 bits (184), Expect = 3.035e-42
Identity = 31/32 (96.88%), Postives = 32/32 (100.00%), Query Frame = 1 
Query:  514 KIADIPPDEYSWRKYGQKPIKGSPYPRGYYKC 609
            KIADIPPDEYSWRKYGQKPIKGSP+PRGYYKC
Sbjct:  240 KIADIPPDEYSWRKYGQKPIKGSPHPRGYYKC 271

HSP 3 Score: 29.261 bits (64), Expect = 3.035e-42
Identity = 11/17 (64.71%), Postives = 17/17 (100.00%), Query Frame = 2 
Query:  467 KENRVKKTIRVPAISSK 517
            ++NR+K+T+RVPAIS+K
Sbjct:  224 RKNRMKRTVRVPAISAK 240
[Close]
BLAST of CF504018 vs. ExPASy Swiss-Prot
Match: WRKY7_ARATH (Probable WRKY transcription factor 7 OS=Arabidopsis thaliana GN=WRKY7 PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.929e-17
Identity = 28/32 (87.50%), Postives = 31/32 (96.88%), Query Frame = 1 
Query:  514 KIADIPPDEYSWRKYGQKPIKGSPYPRGYYKC 609
            K+ADIP DE+SWRKYGQKPIKGSP+PRGYYKC
Sbjct:  275 KMADIPSDEFSWRKYGQKPIKGSPHPRGYYKC 306

HSP 2 Score: 38.5058 bits (88), Expect = 1.929e-17
Identity = 19/63 (30.16%), Postives = 34/63 (53.97%), Query Frame = 3 
Query:  306 FLAPQAPAVSAGKPPLAAQPYKKRCQDHKDHSDDLSGKFSGSTSGNNKCHCSKRRKTE*RKLL 494
            F  P   + S GKPPL++   K+RC                ++S +++CHCSK+RK+  ++++
Sbjct:  221 FTNPSQLSGSRGKPPLSSASLKRRC----------------NSSPSSRCHCSKKRKSRVKRVI 267
[Close]
BLAST of CF504018 vs. ExPASy Swiss-Prot
Match: WRK21_ARATH (Probable WRKY transcription factor 21 OS=Arabidopsis thaliana GN=WRKY21 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.021e-17
Identity = 30/32 (93.75%), Postives = 32/32 (100.00%), Query Frame = 1 
Query:  514 KIADIPPDEYSWRKYGQKPIKGSPYPRGYYKC 609
            K+ADIPPD+YSWRKYGQKPIKGSPYPRGYYKC
Sbjct:  307 KVADIPPDDYSWRKYGQKPIKGSPYPRGYYKC 338

HSP 2 Score: 26.5646 bits (57), Expect = 2.021e-17
Identity = 10/18 (55.56%), Postives = 18/18 (100.00%), Query Frame = 2 
Query:  464 EKENRVKKTIRVPAISSK 517
            ++++RV+++IRVPAIS+K
Sbjct:  290 KRKHRVRRSIRVPAISNK 307

HSP 3 Score: 25.409 bits (54), Expect = 2.021e-17
Identity = 24/75 (32.00%), Postives = 35/75 (46.67%), Query Frame = 3 
Query:  264 DGSVSNGKQGGSSIFLAPQAPAVSAGKPPLAAQPYKKRCQDHKDHSDDLSGKFSGSTSGNNKCHCSKRRKTE*RK 488
            DGSV+N +   S  F  P     S  +  L +   K++C    D    L     GS+S   +CHC+K+RK   R+
Sbjct:  236 DGSVANIEGKNSFHFGVPS----STDQNSLHS---KRKCPLKGDEHGSLK---CGSSS---RCHCAKKRKHRVRR 297
[Close]
BLAST of CF504018 vs. ExPASy Swiss-Prot
Match: WRK74_ARATH (Probable WRKY transcription factor 74 OS=Arabidopsis thaliana GN=WRKY74 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.033e-17
Identity = 31/32 (96.88%), Postives = 32/32 (100.00%), Query Frame = 1 
Query:  514 KIADIPPDEYSWRKYGQKPIKGSPYPRGYYKC 609
            KIADIPPDEYSWRKYGQKPIKGSP+PRGYYKC
Sbjct:  256 KIADIPPDEYSWRKYGQKPIKGSPHPRGYYKC 287

HSP 2 Score: 27.335 bits (59), Expect = 2.033e-17
Identity = 14/31 (45.16%), Postives = 16/31 (51.61%), Query Frame = 3 
Query:  384 DH-KDHSDDLSGKFSGSTSGNNKCHCSKRRK 473
            DH   HS   S   S      +KCHCSK+RK
Sbjct:  211 DHISQHSRRTSCSGSLKCGSKSKCHCSKKRK 241

HSP 3 Score: 25.0238 bits (53), Expect = 2.033e-17
Identity = 10/18 (55.56%), Postives = 17/18 (94.44%), Query Frame = 2 
Query:  464 EKENRVKKTIRVPAISSK 517
            +++ RVK++I+VPAIS+K
Sbjct:  239 KRKLRVKRSIKVPAISNK 256
[Close]
BLAST of CF504018 vs. ExPASy Swiss-Prot
Match: WRK39_ARATH (Probable WRKY transcription factor 39 OS=Arabidopsis thaliana GN=WRKY39 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 9.584e-17
Identity = 31/32 (96.88%), Postives = 32/32 (100.00%), Query Frame = 1 
Query:  514 KIADIPPDEYSWRKYGQKPIKGSPYPRGYYKC 609
            KIADIPPDEYSWRKYGQKPIKGSP+PRGYYKC
Sbjct:  256 KIADIPPDEYSWRKYGQKPIKGSPHPRGYYKC 287

HSP 2 Score: 25.0238 bits (53), Expect = 9.584e-17
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  444 NKCHCSKRRK 473
            +KCHCSK+RK
Sbjct:  232 SKCHCSKKRK 241

HSP 3 Score: 25.0238 bits (53), Expect = 9.584e-17
Identity = 10/18 (55.56%), Postives = 17/18 (94.44%), Query Frame = 2 
Query:  464 EKENRVKKTIRVPAISSK 517
            +++ RVK++I+VPAIS+K
Sbjct:  239 KRKLRVKRSIKVPAISNK 256
[Close]
BLAST of CF504018 vs. ExPASy Swiss-Prot
Match: WRK15_ARATH (Probable WRKY transcription factor 15 OS=Arabidopsis thaliana GN=WRKY15 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 7.607e-16
Identity = 27/32 (84.38%), Postives = 32/32 (100.00%), Query Frame = 1 
Query:  514 KIADIPPDEYSWRKYGQKPIKGSPYPRGYYKC 609
            K++D+PPD+YSWRKYGQKPIKGSP+PRGYYKC
Sbjct:  234 KMSDVPPDDYSWRKYGQKPIKGSPHPRGYYKC 265

HSP 2 Score: 31.187 bits (69), Expect = 7.607e-16
Identity = 13/35 (37.14%), Postives = 23/35 (65.71%), Query Frame = 3 
Query:  390 KDHSDDLSGKFSGSTSGNNKCHCSKRRKTE*RKLL 494
            K +S++L      S S + +CHCSK+RK + R+++
Sbjct:  192 KCNSENLLTGKCASASSSGRCHCSKKRKIKQRRII 226
[Close]
BLAST of CF504018 vs. ExPASy Swiss-Prot
Match: WRKY1_MAIZE (Protein WRKY1 OS=Zea mays PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.279e-14
Identity = 28/32 (87.50%), Postives = 31/32 (96.88%), Query Frame = 1 
Query:  514 KIADIPPDEYSWRKYGQKPIKGSPYPRGYYKC 609
            K+ADIP DE+SWRKYGQKPIKGSP+PRGYYKC
Sbjct:  326 KVADIPADEFSWRKYGQKPIKGSPHPRGYYKC 357

HSP 2 Score: 27.335 bits (59), Expect = 2.279e-14
Identity = 12/43 (27.91%), Postives = 23/43 (53.49%), Query Frame = 3 
Query:  369 KKRCQDHKDHSDDLSGKFSGSTSGNNKCHCSKRRKTE*RKLLE 497
            ++RC   +D +        G  +  ++CHCSK+RK   R+ ++
Sbjct:  285 RRRCAGREDGT--------GRCATGSRCHCSKKRKLRIRRSIK 319
The following BLAST results are available for this feature:
BLAST of CF504018 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 8
Match NameE-valueIdentityDescription
WRK17_ARATH6.278e-4351.61Probable WRKY transcription factor 17 OS=Arabidops... [more]
WRK11_ARATH3.035e-4252.99Probable WRKY transcription factor 11 OS=Arabidops... [more]
WRKY7_ARATH1.929e-1787.50Probable WRKY transcription factor 7 OS=Arabidopsi... [more]
WRK21_ARATH2.021e-1793.75Probable WRKY transcription factor 21 OS=Arabidops... [more]
WRK74_ARATH2.033e-1796.88Probable WRKY transcription factor 74 OS=Arabidops... [more]
WRK39_ARATH9.584e-1796.88Probable WRKY transcription factor 39 OS=Arabidops... [more]
WRK15_ARATH7.607e-1684.38Probable WRKY transcription factor 15 OS=Arabidops... [more]
WRKY1_MAIZE2.279e-1487.50Protein WRKY1 OS=Zea mays PE=1 SV=1[more]
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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-506 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-12_D06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504018 ID=CF504018; Name=CF504018; organism=Citrus sinensis; type=EST; length=610bp
CTTCATCTGCATCTGCGCCAGCCGCCGCCGCCTCAGGCAACTCTCCGCAC
ACACAGACCTTAACTCTTACTCCGCCTGCTCCTACGATGGCTGTCGCTCC
CTCTACGGCGAGTTACGTCCAGTCACAGCCGCACAGTCTGACTCTTGATT
TTACAAAACCTAGTTTGTTTAGCGGAAACGTCAAGAGCACTGAGCTCGAG
TTTTCCAAAGACAGCTTCTGTGTGTCGTCGAACTCGTCCTTCATGTCCTC
TGCTATCACCGGAGACGGCAGCGTTTCGAACGGGAAGCAAGGAGGATCTT
CGATCTTCCTGGCGCCACAAGCTCCTGCTGTTTCCGCTGGAAAGCCGCCG
CTCGCGGCTCAGCCTTACAAGAAGAGGTGTCAAGATCATAAAGATCATTC
CGATGATCTTTCCGGCAAGTTCTCCGGCTCCACCTCTGGCAACAACAAGT
GTCACTGCTCTAAGAGAAGGAAAACCGAGTAAAGAAAACTATTAGAGTGC
CGGCGATTAGTTCAAAATAGCCGATATTCCACCGGACGAGTATTCTTGGA
GGAAGTATGGGCAAAAGCCGATCAAGGGCTCACCATACCCACGCGGCTAT
TACAAGTGCA
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