CF504058

Overview
NameCF504058
Unique NameCF504058
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length626
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_CYACA (Acetolactate synthase small subunit OS=Cyanidium caldarium GN=ilvH PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 8.161e-25
Identity = 43/83 (51.81%), Postives = 65/83 (78.31%), Query Frame = 1 
Query:  232 HTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDITHLPFAERE 480
            +TLS+LV +  GVL  + G+ +RRG+NI+SLAVGPAE++G+SRIT VVPG D ++ +L + L+KL+++ +V +IT +P  ERE
Sbjct:    3 YTLSVLVEDEAGVLTRIAGLFARRGFNIESLAVGPAEQKGISRITMVVPGDDRTVEQLTKQLYKLVNILKVDNITEIPCVERE 85

HSP 2 Score: 40.817 bits (94), Expect = 8.161e-25
Identity = 17/38 (44.74%), Postives = 30/38 (78.95%), Query Frame = 3 
Query:  504 NTAARRDVLDIAKIFRARAVDVSDHTITLELTGDLTKL 617
            +T++R ++L I ++FRA+ VD+S++ + LELTGD  K+
Sbjct:   94 STSSRPEILSILQVFRAKVVDLSENLLVLELTGDPGKV 131
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_GALSU (Acetolactate synthase small subunit OS=Galdieria sulphuraria GN=ilvH PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.796e-24
Identity = 43/83 (51.81%), Postives = 64/83 (77.11%), Query Frame = 1 
Query:  232 HTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDITHLPFAERE 480
            HTLS+LV +  GVL  ++G+ +RRG+NI SLAVGPAEK G+SRIT V+ G +  I ++ + L+KL+++ ++QDIT++P  ERE
Sbjct:    3 HTLSVLVEDESGVLTRISGLFARRGFNIDSLAVGPAEKAGISRITMVIRGDNRIIEQITKHLYKLVNVLKIQDITNIPCVERE 85

HSP 2 Score: 41.2022 bits (95), Expect = 1.796e-24
Identity = 19/34 (55.88%), Postives = 26/34 (76.47%), Query Frame = 3 
Query:  516 RRDVLDIAKIFRARAVDVSDHTITLELTGDLTKL 617
            R ++L+IA IFRAR VD+S+  I LE+TGD  K+
Sbjct:   98 RNEILEIANIFRARVVDLSESFIILEITGDPGKI 131
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_SYNY3 (Acetolactate synthase small subunit OS=Synechocystis sp. (strain PCC 6803) GN=ilvH PE=3 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 1.799e-24
Identity = 44/83 (53.01%), Postives = 62/83 (74.70%), Query Frame = 1 
Query:  232 HTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDITHLPFAERE 480
            HTLS+LV +  GVL  + G+ +RRG+NI+SLAVG AE+  +SRIT VVPG + +I +L + L+KL+++ +VQDIT  P  ERE
Sbjct:    3 HTLSVLVEDEAGVLTRIAGLFARRGFNIESLAVGSAEQGDVSRITMVVPGDENTIEQLTKQLYKLVNVIKVQDITETPCVERE 85

HSP 2 Score: 43.5134 bits (101), Expect = 1.799e-24
Identity = 18/43 (41.86%), Postives = 31/43 (72.09%), Query Frame = 3 
Query:  492 KNCCNTAARRDVLDIAKIFRARAVDVSDHTITLELTGDLTKLL 620
            K   N   R +V+++A++FRAR VD+S+ T+T+E+ GD  K++
Sbjct:   90 KVSANAPNRAEVIELAQVFRARIVDISEDTVTIEVVGDPGKMV 132
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_GUITH (Acetolactate synthase small subunit OS=Guillardia theta GN=ilvH PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 3.049e-24
Identity = 45/83 (54.22%), Postives = 64/83 (77.11%), Query Frame = 1 
Query:  232 HTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDITHLPFAERE 480
            HTLS+LV +  GVL  + G+ +RRG+NI+SLAVGP EK G+SRIT VVP  + +I +L + L+KL+++ +V+DIT+LP  ERE
Sbjct:    3 HTLSVLVEDESGVLTRIAGLFARRGFNIESLAVGPTEKLGISRITMVVPSDERTIEQLTKQLYKLVNILKVEDITNLPTVERE 85

HSP 2 Score: 37.7354 bits (86), Expect = 3.049e-24
Identity = 17/39 (43.59%), Postives = 27/39 (69.23%), Query Frame = 3 
Query:  504 NTAARRDVLDIAKIFRARAVDVSDHTITLELTGDLTKLL 620
            +T  R + LDI  IFRA+ VD+S+  + +E+TGD  K++
Sbjct:   94 STFERTEALDIINIFRAKVVDLSEDFLIIEVTGDPGKIV 132
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_PORYE (Acetolactate synthase small subunit OS=Porphyra yezoensis GN=ilvH PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.138e-23
Identity = 44/83 (53.01%), Postives = 65/83 (78.31%), Query Frame = 1 
Query:  232 HTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDITHLPFAERE 480
            HTLS+LV +  GVL+ ++G+ +RRG+NI SLAVGPAE+ G+SRIT VV G + +I +L + L+KL+++  VQD+T++P  ERE
Sbjct:    3 HTLSVLVQDEAGVLSRISGLFARRGFNIASLAVGPAEQIGVSRITMVVQGDNRTIEQLTKQLYKLVNILNVQDVTNIPSVERE 85

HSP 2 Score: 38.1206 bits (87), Expect = 1.138e-23
Identity = 15/39 (38.46%), Postives = 28/39 (71.79%), Query Frame = 3 
Query:  504 NTAARRDVLDIAKIFRARAVDVSDHTITLELTGDLTKLL 620
            N+  R + L+I +IFRA+ VD+++  + +E+TGD  K++
Sbjct:   94 NSQTRTEALEIVRIFRAKIVDIAEDLLIIEVTGDPGKIV 132
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_PORPU (Acetolactate synthase small subunit OS=Porphyra purpurea GN=ilvH PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.480e-23
Identity = 44/83 (53.01%), Postives = 65/83 (78.31%), Query Frame = 1 
Query:  232 HTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDITHLPFAERE 480
            HTLS+LV +  GVL+ ++G+ +RRG+NI SLAVGPAE+ G+SRIT VV G + +I +L + L+KL+++  VQD+T++P  ERE
Sbjct:    3 HTLSVLVQDEAGVLSRISGLFARRGFNIASLAVGPAEQIGVSRITMVVQGDNRTIEQLTKQLYKLVNILNVQDVTNIPSVERE 85

HSP 2 Score: 37.7354 bits (86), Expect = 1.480e-23
Identity = 17/39 (43.59%), Postives = 27/39 (69.23%), Query Frame = 3 
Query:  504 NTAARRDVLDIAKIFRARAVDVSDHTITLELTGDLTKLL 620
            N+  R + L+I KIFRA  VD+++  I +E+TGD  K++
Sbjct:   94 NSQNRIEALEIVKIFRANVVDIAEDLIIVEVTGDPGKIV 132
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_ARCFU (Probable acetolactate synthase small subunit OS=Archaeoglobus fulgidus GN=ilvH PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 5.885e-18
Identity = 36/75 (48.00%), Postives = 55/75 (73.33%), Query Frame = 1 
Query:  232 HTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDIT 456
            HT+++LV N PGVL  V G+  RRG+NI+SL VG  E++ LSR+T VV G D+ + ++++ L+KLI+  +V +IT
Sbjct:    3 HTIAVLVENKPGVLARVAGLFRRRGFNIESLTVGTTERDDLSRMTIVVEGDDKVVEQVIKQLNKLIETIKVSEIT 77

HSP 2 Score: 34.6538 bits (78), Expect = 5.885e-18
Identity = 15/34 (44.12%), Postives = 24/34 (70.59%), Query Frame = 3 
Query:  516 RRDVLDIAKIFRARAVDVSDHTITLELTGDLTKL 617
            R +++++  IFRAR VDVS  +  +E+TGD  K+
Sbjct:   97 RGEIVELTNIFRARIVDVSRDSFIIEVTGDEDKV 130
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_MYCLE (Acetolactate synthase small subunit OS=Mycobacterium leprae GN=ilvH PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 7.627e-18
Identity = 37/75 (49.33%), Postives = 54/75 (72.00%), Query Frame = 1 
Query:  220 GLRSHTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEV 444
            GL +HTLS+LV +TPGVL  V  + SRRG+NI+SLAVG  E + +SR+T VV   +  + ++ + LHKLI++ +V
Sbjct:    3 GLPTHTLSVLVEDTPGVLARVAALFSRRGFNIESLAVGATECKTMSRMTIVVSAEETPLEQVTKQLHKLINVIKV 77

HSP 2 Score: 37.7354 bits (86), Expect = 7.627e-18
Identity = 19/53 (35.85%), Postives = 31/53 (58.49%), Query Frame = 3 
Query:  459 LAICRARADINKNCCNTAARRDVLDIAKIFRARAVDVSDHTITLELTGDLTKL 617
            LA+ + RA+          R  V++   +FRAR +DVS  ++T+E TGD +K+
Sbjct:   90 LALIKVRAE-------AGTRSQVIEAVHLFRARVIDVSLESLTVEATGDCSKI 135
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_AQUAE (Acetolactate synthase small subunit OS=Aquifex aeolicus GN=ilvH PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.283e-17
Identity = 35/86 (40.70%), Postives = 56/86 (65.12%), Query Frame = 1 
Query:  223 LRSHTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDITHLPFAERE 480
            +R H +++ V N  GVL  +  +I+ +GYNI+ L+VG   ++G+SR+T  V G D  I ++V+ L +LID  +V D+T +P  ERE
Sbjct:   25 VRKHIITVKVRNEMGVLARIATLIAGKGYNIEGLSVGETHEKGISRMTIEVIGDDIVIEQVVKQLRRLIDTLKVSDLTDVPHVERE 110

HSP 2 Score: 39.6614 bits (91), Expect = 1.283e-17
Identity = 19/38 (50.00%), Postives = 27/38 (71.05%), Query Frame = 3 
Query:  504 NTAARRDVLDIAKIFRARAVDVSDHTITLELTGDLTKL 617
            ++ AR +VL I +IFR + VDVS  T T+E+TGD  K+
Sbjct:  120 SSRARDEVLRITEIFRGKVVDVSPDTYTIEVTGDEDKI 157
[Close]
BLAST of CF504058 vs. ExPASy Swiss-Prot
Match: ILVH_METTH (Probable acetolactate synthase small subunit OS=Methanobacterium thermoautotrophicum GN=ilvH PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.781e-16
Identity = 32/84 (38.10%), Postives = 56/84 (66.67%), Query Frame = 1 
Query:  229 SHTLSMLVNNTPGVLNIVTGVISRRGYNIQSLAVGPAEKEGLSRITTVVPGTDESIGKLVQLLHKLIDLHEVQDITHLPFAERE 480
            +H +S LV + PGVL  V G+ +RRG+NI+++ VG +E  G++R+T +  G D  + ++ + L+KLID+ +V+D+      +RE
Sbjct:    8 THIISALVEHKPGVLQRVAGLFTRRGFNIENITVGESETPGIARMTIIARGDDRVLEQITKQLNKLIDVIKVRDLEPAATVKRE 91

HSP 2 Score: 35.4242 bits (80), Expect = 1.781e-16
Identity = 15/34 (44.12%), Postives = 22/34 (64.71%), Query Frame = 3 
Query:  516 RRDVLDIAKIFRARAVDVSDHTITLELTGDLTKL 617
            R +++    IFR R VDVS   +T+E+TGD  K+
Sbjct:  105 RSEIIQYTNIFRGRIVDVSPDALTVEVTGDSEKI 138
The following BLAST results are available for this feature:
BLAST of CF504058 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 21
Match NameE-valueIdentityDescription
ILVH_CYACA8.161e-2551.81Acetolactate synthase small subunit OS=Cyanidium c... [more]
ILVH_GALSU1.796e-2451.81Acetolactate synthase small subunit OS=Galdieria s... [more]
ILVH_SYNY31.799e-2453.01Acetolactate synthase small subunit OS=Synechocyst... [more]
ILVH_GUITH3.049e-2454.22Acetolactate synthase small subunit OS=Guillardia ... [more]
ILVH_PORYE1.138e-2353.01Acetolactate synthase small subunit OS=Porphyra ye... [more]
ILVH_PORPU1.480e-2353.01Acetolactate synthase small subunit OS=Porphyra pu... [more]
ILVH_ARCFU5.885e-1848.00Probable acetolactate synthase small subunit OS=Ar... [more]
ILVH_MYCLE7.627e-1849.33Acetolactate synthase small subunit OS=Mycobacteri... [more]
ILVH_AQUAE1.283e-1740.70Acetolactate synthase small subunit OS=Aquifex aeo... [more]
ILVH_METTH1.781e-1638.10Probable acetolactate synthase small subunit OS=Me... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-546 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-12_H06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504058 ID=CF504058; Name=CF504058; organism=Citrus sinensis; type=EST; length=626bp
GCTCCCTTTTGGAACTTTTCAGCAGCCTCTTATCCAGATCTTGAAGAGAA
AAAGCGAACTGGTATTTTTATACCAGAAACTAATCAGTCTCTAAATGGAA
ATGCAAATGTCGATACATCCTCAAGGGGTGATGTATATCCTGTGGAGCCT
TATGATGACTTTTCAGCAAATCAAGTTCTTGATGCCCATTGGGGTATTCT
CTATGATGAAGATCCAAGTGGCCTCCGGTCACACACTTTATCTATGCTCG
TGAATAACACTCCCGGTGTTCTCAACATTGTTACCGGGGTCATATCACGA
AGAGGTTACAACATTCAGAGTCTTGCTGTGGGGCCTGCTGAAAAGGAGGG
TCTTTCTCGCATCACAACTGTTGTTCCTGGAACTGATGAGTCAATTGGCA
AGTTGGTTCAGCTATTGCACAAGCTAATAGATCTCCACGAGGTTCAAGAT
ATAACTCACTTGCCATTTGCCGAGCGAGAGCTGATATTAATAAAAATTGC
TGTAATACTGCTGCTAGGAGGGATGTCCTTGACATTGCCAAAATATTCCG
GGCCAGAGCAGTTGACGTGTCTGATCATACAATTACTCTTGAGCTTACAG
GCGATTTAACAAAATTATTGCATTGC
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