CF504235 | Citrus Genome Database

Overview


Name	CF504235
Unique Name	CF504235
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	540

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig4551	contig	Csv1_Contig4551:111..647.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: G11A_ORYSJ (Protein kinase G11A OS=Oryza sativa subsp. japonica GN=Os06g0291600 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 3.878e-24
Identity = 53/66 (80.30%), Postives = 57/66 (86.36%), Query Frame = 2

Query:  173 GSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRGAT 370
            G F    LFGKTPFKGS NRATL+NV+GQPLRFPE PVVSFSARDLI+GLLVKEPQ RL C+RGAT
Sbjct:  460 GIFLYELLFGKTPFKGSGNRATLFNVIGQPLRFPEYPVVSFSARDLIRGLLVKEPQQRLGCKRGAT 525

HSP 2 Score: 89.7373 bits (221), Expect = 9.387e-18
Identity = 60/156 (38.46%), Postives = 78/156 (50.00%), Query Frame = 1

Query:   58 LLSQPMPAPCLLWEHT-EYLAPEIIKGEGHGSAVDWWTFGIFLYELPVR*NSF*RI*E*GHLV*CCGATVAISRVTCC*FFSKGFDKGLTSERAPE*ISLQTRSNPRSSNIPSFKV*IGH*FRCASPPEIPKPVEIVHSPAPALSTKAKAVAAGNA 522
            L+++P  A  + +  T EYLAPEIIKGEGHGSAVDWWTFGIFLYEL      F        L    G  +         F ++   +GL  +   + +  + R        P F+       RCASPPE+P+PVEI   P   +ST   A A  +A
Sbjct:  421 LIAEPSDARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVIGQPLRFPEYPVVSFSARDLIRGLLVKEPQQRLGCK-RGATEIKQHPFFEGVNWALIRCASPPEVPRPVEIERPPKQPVSTSEPAAAPSDA 575

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: G11A_ORYSI (Protein kinase G11A OS=Oryza sativa subsp. indica GN=OsI_021818 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 3.878e-24
Identity = 53/66 (80.30%), Postives = 57/66 (86.36%), Query Frame = 2

Query:  173 GSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRGAT 370
            G F    LFGKTPFKGS NRATL+NV+GQPLRFPE PVVSFSARDLI+GLLVKEPQ RL C+RGAT
Sbjct:  460 GIFLYELLFGKTPFKGSGNRATLFNVIGQPLRFPEYPVVSFSARDLIRGLLVKEPQQRLGCKRGAT 525

HSP 2 Score: 89.7373 bits (221), Expect = 9.387e-18
Identity = 60/156 (38.46%), Postives = 78/156 (50.00%), Query Frame = 1

Query:   58 LLSQPMPAPCLLWEHT-EYLAPEIIKGEGHGSAVDWWTFGIFLYELPVR*NSF*RI*E*GHLV*CCGATVAISRVTCC*FFSKGFDKGLTSERAPE*ISLQTRSNPRSSNIPSFKV*IGH*FRCASPPEIPKPVEIVHSPAPALSTKAKAVAAGNA 522
            L+++P  A  + +  T EYLAPEIIKGEGHGSAVDWWTFGIFLYEL      F        L    G  +         F ++   +GL  +   + +  + R        P F+       RCASPPE+P+PVEI   P   +ST   A A  +A
Sbjct:  421 LIAEPSDARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVIGQPLRFPEYPVVSFSARDLIRGLLVKEPQQRLGCK-RGATEIKQHPFFEGVNWALIRCASPPEVPRPVEIERPPKQPVSTSEPAAAPSDA 575

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: KPK1_PHAVU (Protein kinase PVPK-1 OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.925e-23
Identity = 52/66 (78.79%), Postives = 58/66 (87.88%), Query Frame = 2

Query:  173 GSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRGAT 370
            G F    LFG+TPFKGS NRATL+NV+GQPLRFPESP VSF+ARDLI+GLLVKEPQ+RLA RRGAT
Sbjct:  492 GIFLYELLFGRTPFKGSANRATLFNVIGQPLRFPESPTVSFAARDLIRGLLVKEPQHRLAYRRGAT 557

HSP 2 Score: 72.7886 bits (177), Expect = 1.187e-12
Identity = 51/134 (38.06%), Postives = 66/134 (49.25%), Query Frame = 1

Query:   58 LLSQPMPAPCLLWEHT-EYLAPEIIKGEGHGSAVDWWTFGIFLYELPVR*NSF*RI*E*GHLV*CCGATVAISRVTCC*FFSKGFDKGLTSERAPE*ISLQTRSNPRSSNIPSFKV*IGH*FRCASPPEIPKPV 456
            L+++P  A  + +  T EYLAPEIIKGEGHGSAVDWWTFGIFLYEL      F        L    G  +         F ++   +GL   + P+      R        P F+       RCA+PPE+P+ V
Sbjct:  453 LIAEPTNARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGRTPFKGSANRATLFNVIGQPLRFPESPTVSFAARDLIRGLL-VKEPQHRLAYRRGATEIKQHPFFQNVNWALIRCATPPEVPRQV 585

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: PHOT1_ARATH (Phototropin-1 OS=Arabidopsis thaliana GN=PHOT1 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 7.046e-22
Identity = 23/46 (50.00%), Postives = 30/46 (65.22%), Query Frame = 1

Query:   55 LLLSQPMPAPCLLWEHTEYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            + +++PM A        EY+APEII G GH SAVDWW  GI +YE+
Sbjct:  840 IFMAEPMRASNSFVGTEEYIAPEIISGAGHTSAVDWWALGILMYEM 885

HSP 2 Score: 51.6026 bits (122), Expect = 7.046e-22
Identity = 26/66 (39.39%), Postives = 36/66 (54.55%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRGA 367
            LG      L+G TPF+G   + T  NV+ + L+FP S   S   + LI  LL ++P+ RL C  GA
Sbjct:  878 LGILMYEMLYGYTPFRGKTRQKTFTNVLQKDLKFPASIPASLQVKQLIFRLLQRDPKKRLGCFEGA 943

HSP 3 Score: 35.4242 bits (80), Expect = 7.046e-22
Identity = 13/21 (61.90%), Postives = 16/21 (76.19%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISLCKP 434
            E+KQH FF+G+NWALI    P
Sbjct:  945 EVKQHSFFKGINWALIRCTNP 965

HSP 4 Score: 21.557 bits (44), Expect = 7.046e-22
Identity = 6/12 (50.00%), Postives = 9/12 (75.00%), Query Frame = 1

Query:  421 RCASPPEIPKPV 456
            RC +PPE+  P+
Sbjct:  961 RCTNPPELETPI 972

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: KPK7_ARATH (Putative serine/threonine-protein kinase PK7 OS=Arabidopsis thaliana GN=PK7 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.379e-21
Identity = 50/66 (75.76%), Postives = 56/66 (84.85%), Query Frame = 2

Query:  173 GSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRGAT 370
            G F    LFG TPF+G +NRATL+NVVGQPLRFPE P VSF+ARDLI+GLLVKEPQ+RLA RRGAT
Sbjct:  443 GIFLYELLFGITPFRGGDNRATLFNVVGQPLRFPEHPNVSFAARDLIRGLLVKEPQHRLAYRRGAT 508

HSP 2 Score: 73.559 bits (179), Expect = 6.961e-13
Identity = 52/135 (38.52%), Postives = 67/135 (49.63%), Query Frame = 1

Query:   58 LLSQPMPAPCLLWEHT-EYLAPEIIKGEGHGSAVDWWTFGIFLYELPVR*NSF*RI*E*GHLV*CCGATVAISRVTCC*FFSKGFDKGLTSERAPE*ISLQTRSNPRSSNIPSFKV*IGH*FRCASPPEIPKPVE 459
            L+++P  A  + +  T EYLAPEIIKGEGHGSAVDWWTFGIFLYEL      F        L    G  +         F ++   +GL   + P+      R        P F+       RC SPP+IP+PV+
Sbjct:  404 LIAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGITPFRGGDNRATLFNVVGQPLRFPEHPNVSFAARDLIRGLL-VKEPQHRLAYRRGATEIKQHPFFQSVNWALIRCTSPPQIPQPVK 537

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: KN8R_YEAST (Probable serine/threonine-protein kinase YNR047W OS=Saccharomyces cerevisiae GN=YNR047W PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 3.906e-20
Identity = 27/66 (40.91%), Postives = 35/66 (53.03%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRGA 367
            LG      LFG TPFKG     T  N++   + FP +  +S + +DLIK LL K    RL C+ GA
Sbjct:  703 LGILIYEMLFGFTPFKGDNTNETFTNILKNEVSFPNNNEISRTCKDLIKKLLTKNESKRLGCKMGA 768

HSP 2 Score: 52.7582 bits (125), Expect = 3.906e-20
Identity = 19/29 (65.52%), Postives = 25/29 (86.21%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EY+APE+I+G GH +AVDWWT GI +YE+
Sbjct:  682 EYIAPEVIRGNGHTAAVDWWTLGILIYEM 710

HSP 3 Score: 29.261 bits (64), Expect = 3.906e-20
Identity = 12/30 (40.00%), Postives = 19/30 (63.33%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISLCKPARDPETS*D 461
            ++K+HPFF+ V W+L+   +P   P  S D
Sbjct:  770 DVKKHPFFKKVQWSLLRNQEPPLIPVLSED 799

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: NRC2_NEUCR (Serine/threonine-protein kinase nrc-2 OS=Neurospora crassa GN=nrc-2 PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 5.123e-20
Identity = 21/29 (72.41%), Postives = 25/29 (86.21%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EY+APE+IKG GH SAVDWWT GI +YE+
Sbjct:  434 EYIAPEVIKGSGHTSAVDWWTLGILIYEM 462

HSP 2 Score: 51.6026 bits (122), Expect = 5.123e-20
Identity = 26/70 (37.14%), Postives = 39/70 (55.71%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPE---SPVVSFSARDLIKGLLVKEPQNRLACRRGAT 370
            LG      L+G TPFKG    AT  N++ + + FP+   +P +S   + LI+ LL+K+   RL  R GA+
Sbjct:  455 LGILIYEMLYGTTPFKGKNRNATFANILREDIPFPDHAGAPQISNLCKSLIRKLLIKDENRRLGARAGAS 524

HSP 3 Score: 30.0314 bits (66), Expect = 5.123e-20
Identity = 12/21 (57.14%), Postives = 14/21 (66.67%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISLCKP 434
            +IK HPFF+   WALI   KP
Sbjct:  525 DIKTHPFFRTTQWALIRHMKP 545

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: PHOT2_ARATH (Phototropin-2 OS=Arabidopsis thaliana GN=PHOT2 PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 6.554e-20
Identity = 26/66 (39.39%), Postives = 37/66 (56.06%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRGA 367
            LG      L+G+TPF+G   + T  N++ + L FP S  VS   R LI  LL ++P +RL  + GA
Sbjct:  790 LGILLYEMLYGRTPFRGKNRQKTFANILHKDLTFPSSIPVSLVGRQLINTLLNRDPSSRLGSKGGA 855

HSP 2 Score: 51.2174 bits (121), Expect = 6.554e-20
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EY+APEII G GH SA+DWW  GI LYE+
Sbjct:  769 EYIAPEIITGAGHTSAIDWWALGILLYEM 797

HSP 3 Score: 32.7278 bits (73), Expect = 6.554e-20
Identity = 13/21 (61.90%), Postives = 15/21 (71.43%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISLCKP 434
            EIKQH FF+G+NW LI    P
Sbjct:  857 EIKQHAFFRGINWPLIRGMSP 877

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: PPK22_SCHPO (Serine/threonine-protein kinase ppk22 OS=Schizosaccharomyces pombe GN=ppk22 PE=1 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 9.452e-20
Identity = 28/70 (40.00%), Postives = 38/70 (54.29%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPE---SPVVSFSARDLIKGLLVKEPQNRLACRRGAT 370
            LG F    L+G TPFKG    AT  N++   + FPE   +P VS + + LI+ LLVK+   R     GA+
Sbjct:  368 LGIFIYEILYGTTPFKGKNRHATFSNILYSDVSFPEYHGAPNVSSTCKSLIRRLLVKDESKRCGSVAGAS 437

HSP 2 Score: 50.0618 bits (118), Expect = 9.452e-20
Identity = 19/29 (65.52%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EY+APE+I+  GH  AVDWWT GIF+YE+
Sbjct:  347 EYIAPEVIRSCGHTVAVDWWTLGIFIYEI 375

HSP 3 Score: 33.4982 bits (75), Expect = 9.452e-20
Identity = 13/26 (50.00%), Postives = 18/26 (69.23%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALISLCKPARDPE 449
            +IKQHPFF+ + WAL+   KP   P+
Sbjct:  438 DIKQHPFFRHIQWALLRSMKPPIIPK 463

HSP 4 Score: 21.1718 bits (43), Expect = 9.452e-20
Identity = 11/30 (36.67%), Postives = 17/30 (56.67%), Query Frame = 3

Query:   12 PKNEMGNQVSPLPELIAEPTNARSMSFVGT 101
            PK+   +Q  P  +  +  +N R+ SFVGT
Sbjct:  316 PKHSTFSQEKPALDTNSYFSNFRTNSFVGT 345

[Close]

BLAST of CF504235 vs. ExPASy Swiss-Prot
Match: KIN82_YEAST (Probable serine/threonine-protein kinase KIN82 OS=Saccharomyces cerevisiae GN=KIN82 PE=1 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 1.478e-18
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 1

Query:  106 EYLAPEIIKGEGHGSAVDWWTFGIFLYEL 192
            EYLAPE+I+G GH +AVDWWT GI +YE+
Sbjct:  507 EYLAPEVIRGNGHTAAVDWWTLGILIYEM 535

HSP 2 Score: 51.9878 bits (123), Expect = 1.478e-18
Identity = 27/66 (40.91%), Postives = 36/66 (54.55%), Query Frame = 2

Query:  170 LGSFYMSYLFGKTPFKGSENRATLYNVVGQPLRFPESPVVSFSARDLIKGLLVKEPQNRLACRRGA 367
            LG      LFG TPFKG  +  T  N++ + ++FP    VS + +DLIK LL K    RL  + GA
Sbjct:  528 LGILIYEMLFGCTPFKGDNSNETFSNILTKDVKFPHDKEVSKNCKDLIKKLLNKNEAKRLGSKSGA 593

HSP 3 Score: 26.1794 bits (56), Expect = 1.478e-18
Identity = 8/16 (50.00%), Postives = 13/16 (81.25%), Query Frame = 3

Query:  372 EIKQHPFFQGVNWALI 419
            +IK+HPFF+ V W+ +
Sbjct:  595 DIKRHPFFKKVQWSFL 610

The following BLAST results are available for this feature:

BLAST of CF504235 vs. ExPASy Swiss-Prot

BLAST of CF504235 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 25


Match Name	E-value	Identity	Description
G11A_ORYSJ	3.878e-24	80.30	Protein kinase G11A OS=Oryza sativa subsp. japonic...	[more]
G11A_ORYSI	3.878e-24	80.30	Protein kinase G11A OS=Oryza sativa subsp. indica ...	[more]
KPK1_PHAVU	1.925e-23	78.79	Protein kinase PVPK-1 OS=Phaseolus vulgaris PE=2 S...	[more]
PHOT1_ARATH	7.046e-22	50.00	Phototropin-1 OS=Arabidopsis thaliana GN=PHOT1 PE=...	[more]
KPK7_ARATH	1.379e-21	75.76	Putative serine/threonine-protein kinase PK7 OS=Ar...	[more]
KN8R_YEAST	3.906e-20	40.91	Probable serine/threonine-protein kinase YNR047W O...	[more]
NRC2_NEUCR	5.123e-20	72.41	Serine/threonine-protein kinase nrc-2 OS=Neurospor...	[more]
PHOT2_ARATH	6.554e-20	39.39	Phototropin-2 OS=Arabidopsis thaliana GN=PHOT2 PE=...	[more]
PPK22_SCHPO	9.452e-20	40.00	Serine/threonine-protein kinase ppk22 OS=Schizosac...	[more]
KIN82_YEAST	1.478e-18	68.97	Probable serine/threonine-protein kinase KIN82 OS=...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-724 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-16_A01 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504235 ID=CF504235; Name=CF504235; organism=Citrus sinensis; type=EST; length=540bp
AAGCACAGAAGCCTAAGAATGAAATGGGGAACCAAGTGAGCCCATTGCCA
GAACTTATTGCTGAGCCAACCAATGCCCGCTCCATGTCTTTTGTGGGAAC
ACACTGAGTACCTGGCACCGGAAATCATCAAAGGTGAAGGGCATGGCAGT
GCTGTTGATTGGTGGACTTTTGGGATCTTTCTATATGAGCTACCTGTTCG
GTAAAACTCCTTTTAAAGGATCTGAGAATAGGGCCACCTTGTATAATGTT
GTGGGGCAACCGTTGCGATTTCCAGAGTCACCTGTTGTTAGTTTTTCAGC
AAGGGATTTGATAAAGGGCTTACTAGTGAAAGAGCCCCAGAATAGATTAG
CCTGCAGACGAGGAGCAACCCGAGATCAAGCAACATCCCTTCTTTCAAGG
TGTAAATTGGGCATTGATTTCGTTGTGCAAGCCCGCCAGAGATCCCGAAA
CCAGTTGAGATTGTCCATAGTCCAGCCCCAGCATTATCAACTAAGGCAAA
AGCTGTTGCTGCTGGTAATGCTGATCATAATAATTATTTG