CF504368

Overview
NameCF504368
Unique NameCF504368
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length336
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig7217 contig Csv1_Contig7217:969..1302. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_CUCMA (Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 5.061e-47
Identity = 75/81 (92.59%), Postives = 78/81 (96.30%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            SVLPDMFKSTYE+ITKGNP WNQLSVP+  LYSWDPNSTYIHEPPYFK+MTMDPPGAHGVKDAYCLLNFGDSITTDHISPA
Sbjct:  607 SVLPDMFKSTYESITKGNPMWNQLSVPSGTLYSWDPNSTYIHEPPYFKNMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 687

HSP 2 Score: 39.6614 bits (91), Expect = 5.061e-47
Identity = 19/33 (57.58%), Postives = 21/33 (63.64%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      K  P   YLLERGV+R+DFNSYGS
Sbjct:  683 HISPAGSIHKDSPAAKYLLERGVDRKDFNSYGS 715
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_ORYSJ (Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os08g0191100 PE=3 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 9.322e-46
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            SVLPDMFKSTYEAITKGNP WNQL+VP + LYSWDPNSTYIHEPPYFKDMTM PPG HGVK+AYCLLNFGDSITTDHISPA
Sbjct:  607 SVLPDMFKSTYEAITKGNPMWNQLTVPEASLYSWDPNSTYIHEPPYFKDMTMSPPGPHGVKNAYCLLNFGDSITTDHISPA 687

HSP 2 Score: 39.6614 bits (91), Expect = 9.322e-46
Identity = 19/33 (57.58%), Postives = 21/33 (63.64%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      K  P   YLLERGV+R+DFNSYGS
Sbjct:  683 HISPAGSIHKDSPAAKYLLERGVDRKDFNSYGS 715
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_SOLTU (Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 7.781e-45
Identity = 72/81 (88.89%), Postives = 74/81 (91.36%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            SVLPDMFKSTYEAITKGN  WN+LSVP +KLY WDP STYIHEPPYFK MTMDPPG HGVKDAYCLLNFGDSITTDHISPA
Sbjct:  325 SVLPDMFKSTYEAITKGNTMWNELSVPTTKLYQWDPKSTYIHEPPYFKGMTMDPPGPHGVKDAYCLLNFGDSITTDHISPA 405

HSP 2 Score: 39.6614 bits (91), Expect = 7.781e-45
Identity = 19/33 (57.58%), Postives = 21/33 (63.64%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      K  P   YL+ERGV+RRDFNSYGS
Sbjct:  401 HISPAGSIHKDSPAARYLMERGVDRRDFNSYGS 433
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACO2M_ARATH (Aconitate hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=ACO2 PE=1 SV=2)

HSP 1 Score: 161.384 bits (407), Expect = 2.234e-44
Identity = 71/81 (87.65%), Postives = 76/81 (93.83%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            SVLPDMF++TYE+ITKGNP WN+LSVP + LYSWDPNSTYIHEPPYFKDMTMDPPG H VKDAYCLLNFGDSITTDHISPA
Sbjct:  699 SVLPDMFRATYESITKGNPMWNKLSVPENTLYSWDPNSTYIHEPPYFKDMTMDPPGPHNVKDAYCLLNFGDSITTDHISPA 779

HSP 2 Score: 37.3502 bits (85), Expect = 2.234e-44
Identity = 17/33 (51.52%), Postives = 21/33 (63.64%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      K  P   +L+ERGV+R+DFNSYGS
Sbjct:  775 HISPAGNIQKDSPAAKFLMERGVDRKDFNSYGS 807
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACO1_ARATH (Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2)

HSP 1 Score: 152.91 bits (385), Expect = 1.186e-42
Identity = 69/81 (85.19%), Postives = 71/81 (87.65%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            SVLPDMFK+TYEAITKGN  WNQLSV +  LY WDP STYIHEPPYFK MTM PPG HGVKDAYCLLNFGDSITTDHISPA
Sbjct:  607 SVLPDMFKATYEAITKGNSMWNQLSVASGTLYEWDPKSTYIHEPPYFKGMTMSPPGPHGVKDAYCLLNFGDSITTDHISPA 687

HSP 2 Score: 40.0466 bits (92), Expect = 1.186e-42
Identity = 19/33 (57.58%), Postives = 21/33 (63.64%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      K  P   YL+ERGV+RRDFNSYGS
Sbjct:  683 HISPAGSIHKDSPAAKYLMERGVDRRDFNSYGS 715
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_CUCMC (Aconitate hydratase (Fragment) OS=Cucumis melo var. conomon GN=ACO PE=2 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 9.873e-42
Identity = 66/81 (81.48%), Postives = 73/81 (90.12%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            +VLPDMF++TY+AIT+GN TWN LSVP   LYSWDP STYIHEPPYFKDM+M PPG HGVK+AYCLLNFGDSITTDHISPA
Sbjct:  472 NVLPDMFRATYQAITEGNATWNLLSVPEGTLYSWDPTSTYIHEPPYFKDMSMSPPGPHGVKNAYCLLNFGDSITTDHISPA 552

HSP 2 Score: 39.2762 bits (90), Expect = 9.873e-42
Identity = 19/32 (59.38%), Postives = 20/32 (62.50%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYG 328
            H S      K  P   YLLERGV+RRDFNSYG
Sbjct:  548 HISPAGSIHKDSPAAKYLLERGVDRRDFNSYG 579
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACO3M_ARATH (Aconitate hydratase 3, mitochondrial OS=Arabidopsis thaliana GN=ACO3 PE=1 SV=2)

HSP 1 Score: 148.288 bits (373), Expect = 2.550e-39
Identity = 65/81 (80.25%), Postives = 72/81 (88.89%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            SVLP MFKS+YE IT+GNP WN+LS P+S LYSWDPNSTYIHEPPYFK+MT +PPG   VKDAYCLLNFGDS+TTDHISPA
Sbjct:  704 SVLPSMFKSSYETITEGNPLWNELSAPSSTLYSWDPNSTYIHEPPYFKNMTANPPGPREVKDAYCLLNFGDSVTTDHISPA 784

HSP 2 Score: 33.4982 bits (75), Expect = 2.550e-39
Identity = 16/33 (48.48%), Postives = 19/33 (57.58%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      KT P   +L++RGV   DFNSYGS
Sbjct:  780 HISPAGNIQKTSPAAKFLMDRGVISEDFNSYGS 812
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_DICDI (Probable cytoplasmic aconitate hydratase OS=Dictyostelium discoideum GN=aco1 PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.583e-24
Identity = 47/89 (52.81%), Postives = 58/89 (65.17%), Query Frame = 3 
Query:    3 SVLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPARKYPQRQS 269
            SVLPDM++  Y  +  GN +WN+L VP   LY WD  STYIH PP+FK M +       + +AYCLLN GDSITTDHISPA    ++ S
Sbjct:  605 SVLPDMYERVYSNVNDGNKSWNELKVPTGLLYPWDEKSTYIHNPPFFKTMELTVSKRPAITNAYCLLNLGDSITTDHISPAGNINRKSS 693

HSP 2 Score: 25.7942 bits (55), Expect = 1.583e-24
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = 2 
Query:  281 YLLERGVERRDFNSYGS 331
            YL  +GV+  DFN+YGS
Sbjct:  697 YLESKGVKPEDFNTYGS 713
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_BOVIN (Cytoplasmic aconitate hydratase OS=Bos taurus GN=ACO1 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 2.060e-24
Identity = 44/80 (55.00%), Postives = 56/80 (70.00%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+P MFK  Y+ I   N +WN L+ P+ KLY W+P STYI  PP+F+D+T+D      + DAY LLN GDS+TTDHISPA
Sbjct:  604 VIPGMFKEVYQKIETVNESWNALAAPSDKLYCWNPKSTYIKSPPFFEDLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPA 683

HSP 2 Score: 29.6462 bits (65), Expect = 2.060e-24
Identity = 14/33 (42.42%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      +  P   YL  RG+  R+FNSYGS
Sbjct:  679 HISPAGNIARNSPAARYLTNRGLTPREFNSYGS 711
[Close]
BLAST of CF504368 vs. ExPASy Swiss-Prot
Match: ACOC_RAT (Cytoplasmic aconitate hydratase OS=Rattus norvegicus GN=Aco1 PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 2.060e-24
Identity = 43/80 (53.75%), Postives = 56/80 (70.00%), Query Frame = 3 
Query:    6 VLPDMFKSTYEAITKGNPTWNQLSVPASKLYSWDPNSTYIHEPPYFKDMTMDPPGAHGVKDAYCLLNFGDSITTDHISPA 245
            V+P MFK  Y+ I   N +WN L+ P+ KLY+W+P STYI  PP+F+ +T+D      + DAY LLN GDS+TTDHISPA
Sbjct:  604 VIPGMFKEVYQKIETVNKSWNALAAPSEKLYAWNPKSTYIKSPPFFESLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPA 683

HSP 2 Score: 31.187 bits (69), Expect = 2.060e-24
Identity = 15/33 (45.45%), Postives = 17/33 (51.52%), Query Frame = 2 
Query:  233 HFSGEEVSTKTVPLQSYLLERGVERRDFNSYGS 331
            H S      +  P   YL  RG+  RDFNSYGS
Sbjct:  679 HISPAGNIARNSPAARYLTNRGLTPRDFNSYGS 711
The following BLAST results are available for this feature:
BLAST of CF504368 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48
Match NameE-valueIdentityDescription
ACOC_CUCMA5.061e-4792.59Aconitate hydratase, cytoplasmic OS=Cucurbita maxi... [more]
ACOC_ORYSJ9.322e-4690.12Putative aconitate hydratase, cytoplasmic OS=Oryza... [more]
ACOC_SOLTU7.781e-4588.89Aconitate hydratase, cytoplasmic (Fragment) OS=Sol... [more]
ACO2M_ARATH2.234e-4487.65Aconitate hydratase 2, mitochondrial OS=Arabidopsi... [more]
ACO1_ARATH1.186e-4285.19Aconitate hydratase 1 OS=Arabidopsis thaliana GN=A... [more]
ACOC_CUCMC9.873e-4281.48Aconitate hydratase (Fragment) OS=Cucumis melo var... [more]
ACO3M_ARATH2.550e-3980.25Aconitate hydratase 3, mitochondrial OS=Arabidopsi... [more]
ACOC_DICDI1.583e-2452.81Probable cytoplasmic aconitate hydratase OS=Dictyo... [more]
ACOC_BOVIN2.060e-2455.00Cytoplasmic aconitate hydratase OS=Bos taurus GN=A... [more]
ACOC_RAT2.060e-2453.75Cytoplasmic aconitate hydratase OS=Rattus norvegic... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-857 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-17_E07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504368 ID=CF504368; Name=CF504368; organism=Citrus sinensis; type=EST; length=336bp
CCAGTGTGTTGCCAGACATGTTCAAAAGTACCTATGAGGCTATCACAAAG
GGAAATCCCACGTGGAATCAGTTATCTGTACCAGCTAGTAAACTCTATTC
TTGGGACCCTAACTCAACCTACATTCATGAACCTCCTTACTTCAAGGACA
TGACTATGGATCCTCCTGGAGCCCATGGAGTTAAGGATGCCTACTGCTTG
CTGAACTTTGGTGACAGTATCACTACAGATCACATTTCTCCGGCGAGGAA
GTATCCACAAAGACAGTCCCACTGCAAAGTTACCTGCTAGAGCGTGGTGT
GGAACGCAGGGACTTCAATTCTTATGGTAGTCCGTC
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