Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime

Publication Overview
TitleAccumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime
AuthorsLÓPEZ C, CERVERA M, FAGOAGA C, MORENO P, NAVARRO L, FLORES R, PEÑA L
TypeJournal Article
Journal NameMolecular plant pathology
Volume11
Issue1
Year2010
Page(s)33-41
CitationLÓPEZ C, CERVERA M, FAGOAGA C, MORENO P, NAVARRO L, FLORES R, PEÑA L. Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime. Molecular plant pathology. 2010; 11(1):33-41.

Abstract

Mexican lime plants transformed with the 3′-terminal 549 nucleotides of the Citrus tristeza virus (CTV) genome in sense, antisense and intron-hairpin formats were analysed for transgene-derived transcript and short interfering RNA (siRNA) accumulation, and for CTV resistance. Propagations from all sense, antisense and empty-vector transgenic lines were susceptible to CTV, except for a single sense-line plant with a complex transgene integration pattern that showed transgene-derived siRNAs in association with low levels of the transgene-derived transcript. In contrast, nine of 30 intron-hairpin lines showed CTV resistance, with 9%-56% of bud-propagated plants, depending on the line, remaining uninfected on graft inoculation, and the others being susceptible. Although resistance was always associated with the presence of transgene-derived siRNAs, their level in different sense and intron-hairpin transformants was variable irrespective of the response to CTV infection. In intron-hairpin lines with single transgene integration, CTV resistance was correlated with low accumulation of the transgene-derived transcript rather than with high accumulation of transgene-derived siRNAs.
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
Mexican limeMexican limebreeding_research_material
Features
This publication contains information about 7 features:
Feature NameUniquenameType
RF-402/BamHIRF-402/BamHIgenetic_marker
RF-402/XhoIRF-402/XhoIgenetic_marker
RF-403/BamHIRF-403/BamHIgenetic_marker
RF-403/ClaIRF-403/ClaIgenetic_marker
RF-403/EcoRIRF-403/EcoRIgenetic_marker
RF-404/EcoRIRF-404/EcoRIgenetic_marker
RF-405/ClaIRF-405/ClaIgenetic_marker
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2010
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1111/j.1364-3703.2009.00566.x
KeywordsCitrus tristeza virus, nucleotides, small interfering RNA, transgenes
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4311584
PMID: PubMedPMID:20078774