CF832325

Overview
NameCF832325
Unique NameCF832325
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:162..870. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK2_ARATH (S-adenosylmethionine synthetase 2 OS=Arabidopsis thaliana GN=SAM2 PE=1 SV=1)

HSP 1 Score: 413.305 bits (1061), Expect = 6.380e-115
Identity = 198/207 (95.65%), Postives = 202/207 (97.58%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA +DYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE MPLSHVLATK+GARLTEVRKN TC WLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK1_POPTR (S-adenosylmethionine synthetase 1 OS=Populus trichocarpa GN=METK1 PE=2 SV=1)

HSP 1 Score: 413.305 bits (1061), Expect = 6.380e-115
Identity = 197/207 (95.17%), Postives = 203/207 (98.07%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTK K+DYEKIVRDTCR+IGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDETPE+MPLSHVLATKLGARLTEVRKN TC WLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKGKIDYEKIVRDTCRNIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPEYMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK_BRARP (S-adenosylmethionine synthetase OS=Brassica rapa subsp. pekinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 412.535 bits (1059), Expect = 1.088e-114
Identity = 198/207 (95.65%), Postives = 202/207 (97.58%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE MPLSHVLATK+GA+LTEVRKN TC WLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKIGAKLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK5_BRAJU (S-adenosylmethionine synthetase 5 OS=Brassica juncea GN=MSAMS5 PE=2 SV=1)

HSP 1 Score: 412.535 bits (1059), Expect = 1.088e-114
Identity = 198/207 (95.65%), Postives = 202/207 (97.58%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE MPLSHVLATK+GA+LTEVRKN TC WLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKIGAKLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK1_BRAJU (S-adenosylmethionine synthetase 1 OS=Brassica juncea GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 410.994 bits (1055), Expect = 3.166e-114
Identity = 197/207 (95.17%), Postives = 202/207 (97.58%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE MPLSHVLATK+GA+LTEVRKN TC WLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTN+EIA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKIGAKLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNEEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK1_CATRO (S-adenosylmethionine synthetase 1 OS=Catharanthus roseus GN=SAMS1 PE=1 SV=1)

HSP 1 Score: 410.223 bits (1053), Expect = 5.401e-114
Identity = 197/207 (95.17%), Postives = 203/207 (98.07%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGFVSDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKN TC WLRPDGKTQVTVEYYN+NGAMVPVRVHTV+ISTQHDETVTND+IA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNENGAMVPVRVHTVVISTQHDETVTNDQIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK2_CATRO (S-adenosylmethionine synthetase 2 OS=Catharanthus roseus GN=SAMS2 PE=1 SV=1)

HSP 1 Score: 409.838 bits (1052), Expect = 7.054e-114
Identity = 197/207 (95.17%), Postives = 202/207 (97.58%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDPESKVACETCTKTNMVMVFGEITTKA+VDYEKIVRDTCR+IGFVSDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDETPE MPLSHVLATKLGARLTEVRKN TC WLRPDGKTQVTVEYYNDNGAMVP+RVHTVLISTQHDETVTNDEIA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPESKVACETCTKTNMVMVFGEITTKAQVDYEKIVRDTCRAIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNDNGAMVPIRVHTVLISTQHDETVTNDEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK_CARPA (S-adenosylmethionine synthetase OS=Carica papaya GN=SAMS PE=2 SV=1)

HSP 1 Score: 409.453 bits (1051), Expect = 9.212e-114
Identity = 198/207 (95.65%), Postives = 201/207 (97.10%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGFVSDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKN TC WLRPDGKTQVTVEYYN+NGA VPVRVHTVLISTQHDETVTNDEIA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRSIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNENGARVPVRVHTVLISTQHDETVTNDEIA 207
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK4_HORVU (S-adenosylmethionine synthetase 4 OS=Hordeum vulgare GN=SAM4 PE=2 SV=1)

HSP 1 Score: 409.453 bits (1051), Expect = 9.212e-114
Identity = 195/208 (93.75%), Postives = 204/208 (98.08%), Query Frame = 3 
Query:   84 KMETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            ++ETFLFTSESVNEGHPDKLCDQISDAVLDACL +DP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGFVS+DVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKN+TC WLRPDGKTQVTVEY+NDNGAMVP+RVHTVLISTQHDETVTNDEIA
Sbjct:    3 EVETFLFTSESVNEGHPDKLCDQISDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRGIGFVSNDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNATCPWLRPDGKTQVTVEYHNDNGAMVPIRVHTVLISTQHDETVTNDEIA 210
[Close]
BLAST of CF832325 vs. ExPASy Swiss-Prot
Match: METK2_PEA (S-adenosylmethionine synthetase 2 OS=Pisum sativum GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 409.068 bits (1050), Expect = 1.203e-113
Identity = 197/206 (95.63%), Postives = 200/206 (97.09%), Query Frame = 3 
Query:   90 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGARLTEVRKNSTCSWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIA 707
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR+IGFVSDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGA LTEVRKN TC WLRPDGKTQVTVEYYND GAMVP+RVHTVLISTQHDETVTNDEIA
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEDIGAGDQGHMFGYATDETPEFMPLSHVLATKLGASLTEVRKNGTCPWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQHDETVTNDEIA 209
The following BLAST results are available for this feature:
BLAST of CF832325 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK2_ARATH6.380e-11595.65S-adenosylmethionine synthetase 2 OS=Arabidopsis t... [more]
METK1_POPTR6.380e-11595.17S-adenosylmethionine synthetase 1 OS=Populus trich... [more]
METK_BRARP1.088e-11495.65S-adenosylmethionine synthetase OS=Brassica rapa s... [more]
METK5_BRAJU1.088e-11495.65S-adenosylmethionine synthetase 5 OS=Brassica junc... [more]
METK1_BRAJU3.166e-11495.17S-adenosylmethionine synthetase 1 OS=Brassica junc... [more]
METK1_CATRO5.401e-11495.17S-adenosylmethionine synthetase 1 OS=Catharanthus ... [more]
METK2_CATRO7.054e-11495.17S-adenosylmethionine synthetase 2 OS=Catharanthus ... [more]
METK_CARPA9.212e-11495.65S-adenosylmethionine synthetase OS=Carica papaya G... [more]
METK4_HORVU9.212e-11493.75S-adenosylmethionine synthetase 4 OS=Hordeum vulga... [more]
METK2_PEA1.203e-11395.63S-adenosylmethionine synthetase 2 OS=Pisum sativum... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01K23_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01K23, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832325 ID=CF832325; Name=CF832325; organism=Citrus sinensis; type=EST; length=709bp
TTGAGTTCCGCTCTTTTATTGAGGTCTGGTCTTAAGTCTGAATCTTAGAT
CTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACCTTTCT
ATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTGCGACC
AGATCTCTGATGCGGTGCTTGATGCCTGCCTTGAGCAGGATCCTGAGAGC
AAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTCTTTGG
AGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCGCGACA
CTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGATGCCGAT
CACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCTGATATTGCCCA
GGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGCTGGTG
ACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCCCTGAATTTATG
CCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACGGAGGT
TAGGAAGAATAGTACCTGCTCTTGGCTAAGGCCCGATGGGAAGACCCAGG
TCACTGTTGAGTACTACAATGACAATGGTGCCATGGTCCCAGTCCGTGTC
CACACGGTCCTCATTTCAACCCAGCATGATGAGACTGTCACAAATGATGA
AATTGCTGC
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