CF832327

Overview
NameCF832327
Unique NameCF832327
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length646
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3965 contig Csv1_Contig3965:730..1375. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 346.665 bits (888), Expect = 6.217e-95
Identity = 170/215 (79.07%), Postives = 184/215 (85.58%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            YRE VEV KK  N+MF            SRNVVDGSTTF SATVAVVG+GFLARDITFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHS RQF+ +C+IAGTVDFIFGNAAAV QNCDIHAR+PN  Q+NMVTAQGR DPNQNTGIVIQK RIGATSDL  V+GSF TYLGRPWK YSRTV+MQS I+DVI+PAGW+EW GNFAL+T
Sbjct:  311 YRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNT 525
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 345.125 bits (884), Expect = 1.809e-94
Identity = 169/215 (78.60%), Postives = 181/215 (84.19%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            YRE VEV KKK N+MF            SRNVVDGSTTF+SATVA VG+ FLARDITFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVHS RQF+  C I GTVDFIFGNAAAV Q+CDI+ARRPN  Q+NMVTAQGR DPNQNTGIVIQ CRIG TSDLLAVKG+F TYLGRPWK YSRTV+MQSDISDVI P GW+EWSG+FALDT
Sbjct:  314 YRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDT 528
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 344.354 bits (882), Expect = 3.086e-94
Identity = 168/215 (78.14%), Postives = 183/215 (85.12%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            YRE VEV KK  N+MF            SRNVVDGSTTF SAT AVVG+GFLARDITFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHS RQF+ +C+IAGTVDFIFGNAAAV QNCDIHAR+PN  Q+NMVTAQGR DPNQNTGIVIQK RIGATSDL  V+GSF TYLGRPWK YSRTV+MQS I+D+I+PAGW+EW GNFAL+T
Sbjct:  311 YRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNT 525
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 341.273 bits (874), Expect = 2.612e-93
Identity = 168/215 (78.14%), Postives = 180/215 (83.72%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            YRE VEV KKK N+MF            SRNVVDGSTTF+SATVA VG+ FLARDITFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLYVHS RQF+  C+IAGTVDFIFGNAA V Q+CDIHARRPN  Q+NMVTAQGR DPNQNTGIVIQKCRIGATSDL +VKGSF TYLGRPWK YS+TV+MQS ISDVI P GW EW+G FAL+T
Sbjct:  319 YRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNT 533
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME_DAUCA (Pectinesterase OS=Daucus carota PE=1 SV=1)

HSP 1 Score: 335.109 bits (858), Expect = 1.872e-91
Identity = 165/215 (76.74%), Postives = 179/215 (83.26%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            YRE V+VPKKK N+MF            S+NV DGSTTFNSATVA VG GFLARDITFQNTAG +KHQAVALRVGSDLSAFYRCD+LAYQD+LYVHS RQF+ +C IAGTVDFIFGNAA V Q+CDIHARRP   Q+NMVTAQGR DPNQNTGIVIQK RIGATSDL  V+ SF TYLGRPWK YSRTVVMQS I++VINPAGW+ W GNFALDT
Sbjct:   46 YRENVDVPKKKKNIMFLGDGRTSTIITASKNVQDGSTTFNSATVAAVGAGFLARDITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQFFINCFIAGTVDFIFGNAAVVLQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVQSSFPTYLGRPWKEYSRTVVMQSSITNVINPAGWFPWDGNFALDT 260
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 333.183 bits (853), Expect = 7.113e-91
Identity = 164/215 (76.28%), Postives = 177/215 (82.33%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            YRE V+VPKKK N+MF            SRNV DGSTTF+SATV  V    LARDITFQNTAG SKHQAVAL VGSDLSAFYRCDMLAYQDTLYVHS RQF+  C++AGTVDFIFGN AAVFQ+CDIHARRP   Q+NMVTAQGR DPNQNTGIVIQKCRIGATSDL  V+ SF TYLGRPWK YSRTV+MQS I+DVI PAGW+EW+GNFALDT
Sbjct:  310 YRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDT 524
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME_ACTDE (Pectinesterase OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.009e-77
Identity = 137/215 (63.72%), Postives = 166/215 (77.21%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            Y E V+V KKK NLMF            +R+VVDG TTF S+TVAVVG GF+AR I+F+N AGPSKHQAVALR G+D SAFY+C  + YQDTLYVHSLRQFY+ C + GT+DFIFGNAAAV Q C+++AR+PN NQ+N+ TAQGRDDPNQNTGI I  C++ A +DL+ V  SF+TYLGRPWK YSRTV + S +  +I+PAGW EWSG+FAL T
Sbjct:   47 YFEYVDVDKKKTNLMFIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSGADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNENQKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQMESLIDPAGWLEWSGDFALTT 261
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME18_ARATH (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana GN=PME18 PE=1 SV=2)

HSP 1 Score: 277.715 bits (709), Expect = 3.547e-74
Identity = 136/215 (63.26%), Postives = 160/215 (74.42%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            Y+E +++ KKK NLM             S NV+DGSTTF SATVA  GDGF+A+DI FQNTAGP+KHQAVALRV +D +   RC + AYQDTLY H+LRQFY    I GTVDFIFGN+A VFQNCDI AR P   Q+NM+TAQGR+D NQNT I IQKC+I A+SDL  VKGS +T+LGRPWK YSRTV+MQS I + I+PAGW+ W G FAL T
Sbjct:  284 YKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALST 498
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME36_ARATH (Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana GN=PME36 PE=2 SV=1)

HSP 1 Score: 270.396 bits (690), Expect = 5.662e-72
Identity = 131/215 (60.93%), Postives = 157/215 (73.02%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            Y E +E+ +   N+M             +RNV DGSTT+ SAT  V GDGF ARDITF+NTAGP KHQAVALRV SDLS FYRC    YQDTL+ HSLRQFY    I GT+DFIFG+AAAVFQNCDI  RRP  +Q NM+TAQGRDDP+ N+GI IQ  RI A  +  AVKG F++YLGRPWK+YSRTV +++DI ++I+P GW EWSG++AL T
Sbjct:  246 YNEKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITFENTAGPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDRHIYGTIDFIFGDAAAVFQNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRPWKKYSRTVFLKTDIDELIDPRGWREWSGSYALST 460
[Close]
BLAST of CF832327 vs. ExPASy Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 1.261e-71
Identity = 134/215 (62.33%), Postives = 160/215 (74.42%), Query Frame = 2 
Query:    2 YREPVEVPKKKINLMFXXXXXXXXXXXXSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPNQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDISDVINPAGWYEWSGNFALDT 646
            Y E +EV  K  N+MF            S++V  G+TTF SATVAVVGD F+ARDIT +NTAGP+ HQAVALR GSDLS FYRC    YQDTLYVHS RQFY  C I GTVDFIFGNAA V QNC+I AR+P PN+ N +TAQGR DPNQ+TGI+I  CR+ A SDL  V+ S +T+LGRPWK+YSRTV +++ +  +INPAGW EWSG+FAL+T
Sbjct:  240 YNENIEVKLK--NIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNT 451
The following BLAST results are available for this feature:
BLAST of CF832327 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 87
Match NameE-valueIdentityDescription
PME3_CITSI6.217e-9579.07Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME2_ARATH1.809e-9478.60Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 P... [more]
PME1_CITSI3.086e-9478.14Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE... [more]
PME3_ARATH2.612e-9378.14Pectinesterase/pectinesterase inhibitor 3 OS=Arabi... [more]
PME_DAUCA1.872e-9176.74Pectinesterase OS=Daucus carota PE=1 SV=1[more]
PMEU1_SOLLC7.113e-9176.28Pectinesterase/pectinesterase inhibitor U1 OS=Sola... [more]
PME_ACTDE2.009e-7763.72Pectinesterase OS=Actinidia deliciosa PE=1 SV=1[more]
PME18_ARATH3.547e-7463.26Pectinesterase/pectinesterase inhibitor 18 OS=Arab... [more]
PME36_ARATH5.662e-7260.93Probable pectinesterase/pectinesterase inhibitor 3... [more]
PME2_CITSI1.261e-7162.33Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01L01_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01L01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832327 ID=CF832327; Name=CF832327; organism=Citrus sinensis; type=EST; length=646bp
GTACAGAGAACCTGTTGAGGTGCCGAAGAAGAAAATTAATCTTATGTTTA
TTGGAGATGGGAGGACTACTACAATAATCACCGGTAGCAGGAATGTGGTT
GATGGCAGCACTACATTCAACTCTGCAACTGTTGCTGTGGTAGGGGACGG
ATTCTTGGCCAGAGACATAACATTTCAAAACACGGCGGGTCCGTCAAAAC
ATCAAGCCGTGGCACTGAGAGTAGGATCAGATTTATCAGCATTCTATAGG
TGTGACATGCTAGCATACCAAGACACTCTCTATGTACACTCACTTCGCCA
ATTTTATACCAGCTGCATTATAGCAGGCACAGTGGACTTCATTTTCGGAA
ATGCAGCAGCTGTGTTCCAAAACTGTGACATCCATGCTCGCCGCCCCAAT
CCAAACCAAAGGAACATGGTCACGGCACAAGGCCGCGACGACCCGAATCA
GAACACAGGCATAGTGATACAAAAATGCAGGATTGGTGCAACATCAGATT
TACTTGCTGTAAAGGGCAGCTTTGAGACATATTTAGGCAGGCCATGGAAG
CGGTACTCGAGGACTGTTGTGATGCAATCTGATATAAGTGATGTTATTAA
CCCAGCTGGGTGGTATGAATGGAGTGGGAATTTTGCATTGGACACA
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