CF832536

Overview
NameCF832536
Unique NameCF832536
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length651
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRFC_ARATH (Ribosome-recycling factor, chloroplastic OS=Arabidopsis thaliana GN=RRF PE=1 SV=2)

HSP 1 Score: 246.128 bits (627), Expect = 1.155e-64
Identity = 124/146 (84.93%), Postives = 139/146 (95.21%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 440
            SN  MLDKIEVEYYGSPVSLKSIAQI+TPD SSLL+QPYDKSSLK+IEKAIV+SDLG+TPNNDG+VIRL+LP LTS+RRKELSKVVAKQ+EEGKVA+RNIRRDALK+Y+KL+KEKKLSEDNVKDLSSDLQKL D YMKKI+ +YKQ
Sbjct:  122 SNAAMLDKIEVEYYGSPVSLKSIAQISTPDGSSLLLQPYDKSSLKAIEKAIVNSDLGVTPNNDGDVIRLSLPPLTSDRRKELSKVVAKQSEEGKVALRNIRRDALKSYDKLEKEKKLSEDNVKDLSSDLQKLIDVYMKKIEELYKQ 267          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRFC_DAUCA (Ribosome-recycling factor, chloroplastic (Fragment) OS=Daucus carota GN=RRF PE=2 SV=2)

HSP 1 Score: 245.743 bits (626), Expect = 1.508e-64
Identity = 120/146 (82.19%), Postives = 141/146 (96.58%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 440
            SNP MLDKI+VEYYG+P SLKSIAQI+TPDSSSLL+ PYDKSSLK IEKAIV+SDLG+TPNNDG+VIRL++PQLT++RRKELSK+VAKQAEEGKVA+RNIRRDA+K+Y+KL+KEKKLSEDNVKDLSSDLQK+ DEY+KK+DSI+KQ
Sbjct:   74 SNPDMLDKIKVEYYGTPTSLKSIAQISTPDSSSLLVNPYDKSSLKDIEKAIVNSDLGITPNNDGDVIRLSIPQLTADRRKELSKIVAKQAEEGKVALRNIRRDAIKSYDKLEKEKKLSEDNVKDLSSDLQKVIDEYIKKVDSIFKQ 219          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRFC_SPIOL (Ribosome-recycling factor, chloroplastic OS=Spinacia oleracea GN=RRF PE=1 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 3.360e-64
Identity = 122/146 (83.56%), Postives = 141/146 (96.58%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 440
            ++P MLD+IEVEYYG+PVSLKSIAQI+TPD+SSLLI PYDKSSLK+IEKAIV+S LG++PNNDGEVIRL+LP LTS+RRKEL+KVV+K AEEGKVAVRNIRRDALK+YEKL+KEKKLSEDNVKDLS+DLQKLTDEYMKK++SIYKQ
Sbjct:  118 ASPTMLDRIEVEYYGTPVSLKSIAQISTPDASSLLISPYDKSSLKAIEKAIVTSQLGVSPNNDGEVIRLSLPPLTSDRRKELAKVVSKLAEEGKVAVRNIRRDALKSYEKLEKEKKLSEDNVKDLSADLQKLTDEYMKKVESIYKQ 263          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRFC_ORYSJ (Ribosome-recycling factor, chloroplastic OS=Oryza sativa subsp. japonica GN=Os07g0570700 PE=2 SV=2)

HSP 1 Score: 236.884 bits (603), Expect = 7.005e-62
Identity = 116/146 (79.45%), Postives = 139/146 (95.21%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 440
            +NP MLD+IEVEYYG+PV+LKSIAQINTPD++SLLIQPYDKSSLK IEK IV+++LG+TP+NDGEVIR+T+P LTS+RRKEL+K VAK AEEGKVA+RNIRRDA+KAY+KL+KEKKLSEDNVKDLS+DLQK+TDEYMKKI++I KQ
Sbjct:  113 ANPAMLDRIEVEYYGTPVNLKSIAQINTPDATSLLIQPYDKSSLKLIEKTIVAANLGVTPSNDGEVIRVTVPPLTSDRRKELAKTVAKLAEEGKVAIRNIRRDAIKAYDKLEKEKKLSEDNVKDLSADLQKVTDEYMKKIEAIQKQ 258          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRFC_ORYSI (Ribosome-recycling factor, chloroplastic OS=Oryza sativa subsp. indica GN=OsI_26546 PE=1 SV=2)

HSP 1 Score: 236.884 bits (603), Expect = 7.005e-62
Identity = 116/146 (79.45%), Postives = 139/146 (95.21%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYKQ 440
            +NP MLD+IEVEYYG+PV+LKSIAQINTPD++SLLIQPYDKSSLK IEK IV+++LG+TP+NDGEVIR+T+P LTS+RRKEL+K VAK AEEGKVA+RNIRRDA+KAY+KL+KEKKLSEDNVKDLS+DLQK+TDEYMKKI++I KQ
Sbjct:  113 ANPAMLDRIEVEYYGTPVNLKSIAQINTPDATSLLIQPYDKSSLKLIEKTIVAANLGVTPSNDGEVIRVTVPPLTSDRRKELAKTVAKLAEEGKVAIRNIRRDAIKAYDKLEKEKKLSEDNVKDLSADLQKVTDEYMKKIEAIQKQ 258          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRF_SYNJB (Ribosome-recycling factor OS=Synechococcus sp. GN=frr PE=3 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 5.389e-46
Identity = 88/143 (61.54%), Postives = 116/143 (81.12%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 431
            +N  +LD+I+VEYYG P  LK++A + TPDSS+LLIQP+D S+L +IE+AIV+SDLG+TP+NDG+V+RLT+P LT ERRKELSK VAK AEEG+V++RNIRRD + +  K +K  +LSED  K L  D+QKLTD Y+KKID +
Sbjct:   29 ANSSLLDRIQVEYYGVPTPLKALASLTTPDSSTLLIQPFDPSTLAAIERAIVASDLGLTPSNDGKVVRLTIPPLTEERRKELSKQVAKLAEEGRVSIRNIRRDGIDSVRKREKNGELSEDESKSLQDDIQKLTDRYIKKIDEL 171          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRF_ANASP (Ribosome-recycling factor OS=Anabaena sp. (strain PCC 7120) GN=frr PE=3 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 6.587e-44
Identity = 85/143 (59.44%), Postives = 113/143 (79.02%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 431
            +N  +LDK+ V+YYGSP  LKS+A I+TPD++++LIQPYDKSSL  +EKAI  SD+G+TP+NDG VIRL +P LTS+RRKEL K+ AK AEEG+VA+RNIRRDA+ +  KL+K  ++SED  KD    LQKLT++Y  +ID +
Sbjct:   29 ANASLLDKVLVDYYGSPTPLKSLANISTPDATTILIQPYDKSSLNIVEKAISLSDVGLTPSNDGAVIRLNIPPLTSDRRKELVKIAAKYAEEGRVAIRNIRRDAVDSIRKLEKNAEVSEDEAKDQQDKLQKLTNKYTARIDEL 171          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRF_SYNP6 (Ribosome-recycling factor OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=frr PE=3 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 9.512e-43
Identity = 81/143 (56.64%), Postives = 114/143 (79.72%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 431
            +N  +LDKI V+YYGS   LKS+A I+TPD+S+L IQP+D+SS+ +IEKAI  SD+G+TPNNDG++IRL +P LTS+RRKEL K+ AK AEEGKVA+RN+RRDA+ +  KL+K  ++SED+ +D    +QKLTD++  K++ +
Sbjct:   29 ANASLLDKIVVDYYGSETPLKSLASISTPDASTLQIQPFDRSSMGAIEKAISLSDIGLTPNNDGQIIRLNIPPLTSDRRKELVKLAAKYAEEGKVAIRNVRRDAIDSIRKLEKSGEISEDDARDQQDQVQKLTDKFSAKVEQL 171          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRF_SYNE7 (Ribosome-recycling factor OS=Synechococcus elongatus (strain PCC 7942) GN=frr PE=3 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 9.512e-43
Identity = 81/143 (56.64%), Postives = 114/143 (79.72%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 431
            +N  +LDKI V+YYGS   LKS+A I+TPD+S+L IQP+D+SS+ +IEKAI  SD+G+TPNNDG++IRL +P LTS+RRKEL K+ AK AEEGKVA+RN+RRDA+ +  KL+K  ++SED+ +D    +QKLTD++  K++ +
Sbjct:   29 ANASLLDKIVVDYYGSETPLKSLASISTPDASTLQIQPFDRSSMGAIEKAISLSDIGLTPNNDGQIIRLNIPPLTSDRRKELVKLAAKYAEEGKVAIRNVRRDAIDSIRKLEKSGEISEDDARDQQDQVQKLTDKFSAKVEQL 171          
BLAST of CF832536 vs. ExPASy Swiss-Prot
Match: RRF_CYAP4 (Ribosome-recycling factor OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=frr PE=3 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 9.512e-43
Identity = 83/143 (58.04%), Postives = 112/143 (78.32%), Query Frame = 3
Query:    3 SNPVMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 431
            +N  +LD++ VEYYG+   L+S+A I+TPDSS++ IQP+D++SL  IEKAI  SDLG+TPNNDG VIRL +P LTS+RRKEL K  +K AEEGKV++RN+RRDA+ + +K  K  +LSED VKDL   +QKLTD+Y+ K+D +
Sbjct:   29 ANTSLLDRVTVEYYGAETPLRSLANISTPDSSTIAIQPFDRNSLNLIEKAISMSDLGLTPNNDGTVIRLNIPPLTSDRRKELVKTASKLAEEGKVSIRNVRRDAVDSVKKQGKAGELSEDEVKDLQDKIQKLTDKYIAKVDGL 171          
The following BLAST results are available for this feature:
BLAST of CF832536 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RRFC_ARATH1.155e-6484.93Ribosome-recycling factor, chloroplastic OS=Arabid... [more]
RRFC_DAUCA1.508e-6482.19Ribosome-recycling factor, chloroplastic (Fragment... [more]
RRFC_SPIOL3.360e-6483.56Ribosome-recycling factor, chloroplastic OS=Spinac... [more]
RRFC_ORYSJ7.005e-6279.45Ribosome-recycling factor, chloroplastic OS=Oryza ... [more]
RRFC_ORYSI7.005e-6279.45Ribosome-recycling factor, chloroplastic OS=Oryza ... [more]
RRF_SYNJB5.389e-4661.54Ribosome-recycling factor OS=Synechococcus sp. GN=... [more]
RRF_ANASP6.587e-4459.44Ribosome-recycling factor OS=Anabaena sp. (strain ... [more]
RRF_SYNP69.512e-4356.64Ribosome-recycling factor OS=Synechococcus sp. (st... [more]
RRF_SYNE79.512e-4356.64Ribosome-recycling factor OS=Synechococcus elongat... [more]
RRF_CYAP49.512e-4358.04Ribosome-recycling factor OS=Cyanothece sp. (strai... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02A06_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02A06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832536 ID=CF832536|Name=CF832536|organism=Citrus sinensis|type=EST|length=651bp
GATCGAATCCAGTTATGCTAGATAAGATTGAGGTGGAATACTATGGAAGC
CCAGTAAGTTTGAAGAGCATAGCTCAAATCAATACCCCTGATTCAAGTTC
TCTATTGATTCAACCATATGACAAATCCAGCTTAAAGTCTATAGAGAAGG
CCATAGTGAGCTCTGATCTTGGCATGACCCCAAACAATGATGGTGAAGTC
ATACGGTTAACCCTTCCTCAATTGACATCCGAAAGGAGGAAGGAACTGTC
AAAGGTTGTGGCCAAACAGGCTGAAGAAGGGAAGGTGGCAGTTAGGAATA
TAAGAAGAGATGCGTTAAAAGCTTATGAAAAACTTCAGAAGGAGAAGAAG
CTCTCTGAAGACAATGTGAAGGACTTGTCAAGTGATTTACAGAAACTAAC
AGATGAGTATATGAAGAAGATTGATAGCATCTACAAACAGAAAGAGAAGG
AGTTGTTGAAGGTTTAATGATTGTTTACACACAAATGTAATGGTGAGCTC
TCTAGTGAATCTCAGAATGCACTCTTTATTTTTTGGTTTGCCCATAGAAT
GGCATTTTTTGATACTAATGGGAGACACGGGTGGATATTTTGTTATGAAA
TGAAATGTGGAAATTAGTTATTCTAGACTCTTTGTAAAAAAAAAAAAAAA
A
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