CF832740

Overview
NameCF832740
Unique NameCF832740
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA_ARATH (Xylose isomerase OS=Arabidopsis thaliana GN=XYLA PE=2 SV=2)

HSP 1 Score: 250.366 bits (638), Expect = 2.172e-66
Identity = 114/134 (85.07%), Postives = 129/134 (96.27%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGS 403
            WDAATAANFLRKYGLI++FKLNIECNHATLSGH+CHH++ETAR+NGLLGNIDANTGD Q GWDTDQFLTD+GEAT++M+SVIKNGG+APGGFNFDAKLRRESTDVEDLFIAHI GMDT+ARGLR A K++E+GS
Sbjct:  286 WDAATAANFLRKYGLIDEFKLNIECNHATLSGHTCHHELETARINGLLGNIDANTGDAQTGWDTDQFLTDVGEATMVMMSVIKNGGIAPGGFNFDAKLRRESTDVEDLFIAHISGMDTMARGLRNAVKILEEGS 419          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA_HORVU (Xylose isomerase OS=Hordeum vulgare GN=XYLA PE=1 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 6.768e-60
Identity = 106/134 (79.10%), Postives = 119/134 (88.81%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGS 403
            WD AT  +FL+K+GL  +FK+N+ECNHATLSGHSCHH++ETAR+N +LGNIDANTGDPQVGWDTD+FLTDI EATLIM SV+KN GLAPGGFNF AKLRRESTDVEDLFIAHI GMDT+ARG R   KLIEDGS
Sbjct:  288 WDVATTFSFLQKFGLTGEFKINVECNHATLSGHSCHHELETARINDILGNIDANTGDPQVGWDTDEFLTDISEATLIMSSVVKNDGLAPGGFNFYAKLRRESTDVEDLFIAHISGMDTMARGRRNVVKLIEDGS 421          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA_BACA2 (Xylose isomerase OS=Bacillus amyloliquefaciens (strain FZB42) GN=xylA PE=3 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 2.584e-43
Identity = 80/131 (61.07%), Postives = 101/131 (77.10%), Query Frame = 2
Query:    5 DAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIED 397
            DAAT   FL++YGL N FKLN+E NHATL+GH+  H++  AR++GLLG++DAN GDP +GWDTD+F TD+  ATL M  ++KNGGL  GG NFDAK+RR S + +DL  AH+ GMDT ARGL+ A KLIED
Sbjct:  252 DAATTIAFLKQYGLDNHFKLNLEANHATLAGHTFEHELRMARVHGLLGSVDANQGDPLLGWDTDEFPTDLYSATLAMYEILKNGGLGSGGLNFDAKVRRSSFEPDDLLYAHVAGMDTFARGLKAARKLIED 382          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA_LISW6 (Xylose isomerase OS=Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) GN=xylA PE=3 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 5.756e-43
Identity = 81/132 (61.36%), Postives = 102/132 (77.27%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIED 397
            +DAAT   FL+KYGL NDFKLN+E NHATL+GH+  H++  AR    LG+IDAN GD  +GWDTD+F T+I + TL M  +++NGG+APGG NFDAK+RR S ++EDL +AHI GMDT ARGL+ AAKL ED
Sbjct:  243 FDAATTMAFLQKYGLENDFKLNLEANHATLAGHTFEHELNVARTYNALGSIDANQGDLLLGWDTDEFPTNIYDTTLTMYEILQNGGIAPGGINFDAKVRRTSFEMEDLLLAHIAGMDTYARGLKAAAKLTED 374          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA_XANOM (Xylose isomerase OS=Xanthomonas oryzae pv. oryzae (strain MAFF 311018) GN=xylA1 PE=3 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 1.086e-41
Identity = 82/130 (63.08%), Postives = 99/130 (76.15%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLI 391
            +D+AT   FLR++GL  DFKLNIE NHATLSGHS  HD++ A   GLLG+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+
Sbjct:  253 FDSATVIGFLRQHGLDQDFKLNIEANHATLSGHSFEHDLQVASDAGLLGSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVANALL 382          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA_XANC5 (Xylose isomerase OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=xylA1 PE=3 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 1.086e-41
Identity = 82/130 (63.08%), Postives = 99/130 (76.15%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLI 391
            +D+AT   FLR++GL  DFKLNIE NHATLSGHS  HD++ A   GLLG+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+
Sbjct:  253 FDSATVIGFLRQHGLDQDFKLNIEANHATLSGHSFEHDLQVASDAGLLGSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVANALL 382          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA2_XANOR (Xylose isomerase 2 OS=Xanthomonas oryzae pv. oryzae GN=xylA2 PE=3 SV=2)

HSP 1 Score: 168.318 bits (425), Expect = 1.086e-41
Identity = 82/130 (63.08%), Postives = 99/130 (76.15%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLI 391
            +D+AT   FLR++GL  DFKLNIE NHATLSGHS  HD++ A   GLLG+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+
Sbjct:  253 FDSATVIGFLRQHGLDQDFKLNIEANHATLSGHSFEHDLQVASDAGLLGSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVANALL 382          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA2_XANCP (Xylose isomerase 2 OS=Xanthomonas campestris pv. campestris GN=xylA2 PE=3 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 1.086e-41
Identity = 82/130 (63.08%), Postives = 99/130 (76.15%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLI 391
            +D+AT   FLR++GL  DFKLNIE NHATLSGHS  HD++ A   GLLG+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+
Sbjct:  253 FDSATVIGFLRQHGLDQDFKLNIEANHATLSGHSFEHDLQVASDAGLLGSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVADALL 382          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA2_XANC8 (Xylose isomerase 2 OS=Xanthomonas campestris pv. campestris (strain 8004) GN=xylA2 PE=3 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 1.086e-41
Identity = 82/130 (63.08%), Postives = 99/130 (76.15%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLI 391
            +D+AT   FLR++GL  DFKLNIE NHATLSGHS  HD++ A   GLLG+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+
Sbjct:  253 FDSATVIGFLRQHGLDQDFKLNIEANHATLSGHSFEHDLQVASDAGLLGSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVADALL 382          
BLAST of CF832740 vs. ExPASy Swiss-Prot
Match: XYLA2_XANAC (Xylose isomerase 2 OS=Xanthomonas axonopodis pv. citri GN=xylA2 PE=3 SV=2)

HSP 1 Score: 168.318 bits (425), Expect = 1.086e-41
Identity = 82/130 (63.08%), Postives = 99/130 (76.15%), Query Frame = 2
Query:    2 WDAATAANFLRKYGLINDFKLNIECNHATLSGHSCHHDIETARLNGLLGNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLI 391
            +D+AT   FLR++GL  DFKLNIE NHATLSGHS  HD++ A   GLLG+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+
Sbjct:  253 FDSATVIGFLRQHGLDQDFKLNIEANHATLSGHSFEHDLQVASDAGLLGSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVANALL 382          
The following BLAST results are available for this feature:
BLAST of CF832740 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 173
Match NameE-valueIdentityDescription
XYLA_ARATH2.172e-6685.07Xylose isomerase OS=Arabidopsis thaliana GN=XYLA P... [more]
XYLA_HORVU6.768e-6079.10Xylose isomerase OS=Hordeum vulgare GN=XYLA PE=1 S... [more]
XYLA_BACA22.584e-4361.07Xylose isomerase OS=Bacillus amyloliquefaciens (st... [more]
XYLA_LISW65.756e-4361.36Xylose isomerase OS=Listeria welshimeri serovar 6b... [more]
XYLA_XANOM1.086e-4163.08Xylose isomerase OS=Xanthomonas oryzae pv. oryzae ... [more]
XYLA_XANC51.086e-4163.08Xylose isomerase OS=Xanthomonas campestris pv. ves... [more]
XYLA2_XANOR1.086e-4163.08Xylose isomerase 2 OS=Xanthomonas oryzae pv. oryza... [more]
XYLA2_XANCP1.086e-4163.08Xylose isomerase 2 OS=Xanthomonas campestris pv. c... [more]
XYLA2_XANC81.086e-4163.08Xylose isomerase 2 OS=Xanthomonas campestris pv. c... [more]
XYLA2_XANAC1.086e-4163.08Xylose isomerase 2 OS=Xanthomonas axonopodis pv. c... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02F15_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02F15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832740 ID=CF832740; Name=CF832740; organism=Citrus sinensis; type=EST; length=405bp
CTGGGATGCAGCAACAGCAGCTAATTTCTTGCGGAAATATGGCCTCATAA
ATGACTTTAAACTAAACATTGAGTGTAACCATGCCACCCTATCTGGTCAC
AGCTGCCATCATGATATTGAAACTGCAAGACTCAATGGTCTGCTAGGAAA
CATCGATGCAAACACTGGTGACCCTCAAGTTGGTTGGGATACAGATCAAT
TCCTGACAGATATTGGAGAGGCTACTTTGATTATGCTCAGTGTGATTAAA
AATGGAGGTTTGGCACCTGGAGGATTCAACTTTGATGCAAAATTACGAAG
AGAGAGCACTGACGTTGAAGACTTGTTCATTGCTCATATTGTTGGAATGG
ATACCGTTGCTCGTGGACTTCGAACTGCTGCCAAGCTGATTGAGGATGGT
TCTCT
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