CF834747

Overview
NameCF834747
Unique NameCF834747
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length746
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12192 contig Csv1_Contig12192:322..1067. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4_SPIOL (Proteasome subunit alpha type-4 OS=Spinacia oleracea GN=PAC1 PE=2 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 8.039e-87
Identity = 157/187 (83.96%), Postives = 172/187 (91.98%), Query Frame = -2 
Query:  185 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKDDISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMPSGKVKYQVCSPEALSKLLVKHGVTQPAAETS 745
            KMYKIDDHVACAVAGIMSDANILINTARVQAQRYT++YQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWK           QS+LKQDYKDD++RE+AV+LALK LSKTMDSTSLTS+KLELAEV+L+PSGKVKYQV SPE+L++LL + G+TQPAAETS
Sbjct:   64 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTFSYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKATAIGANNQAAQSMLKQDYKDDVTREDAVKLALKALSKTMDSTSLTSEKLELAEVYLLPSGKVKYQVHSPESLNRLLTESGLTQPAAETS 250
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4_ARATH (Proteasome subunit alpha type-4 OS=Arabidopsis thaliana GN=PAC1 PE=1 SV=1)

HSP 1 Score: 317.39 bits (812), Expect = 5.211e-86
Identity = 159/187 (85.03%), Postives = 169/187 (90.37%), Query Frame = -2 
Query:  185 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKDDISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMPSGKVKYQVCSPEALSKLLVKHGVTQPAAETS 745
            KMYKIDDHVACAVAGIMSDANILINTARVQAQRYT+ YQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDK++GFQLYMSDPSGNYGGWK           QSILKQDYKDD +REEAV+LALKVL+KTMDSTSLTS+KLELAEV+L PS  VKY V SPE+L+KLLVKHGVTQPAAETS
Sbjct:   64 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTFMYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYGGWKAAAVGANNQAAQSILKQDYKDDATREEAVELALKVLTKTMDSTSLTSEKLELAEVYLTPSKTVKYHVHSPESLTKLLVKHGVTQPAAETS 250
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4_PETHY (Proteasome subunit alpha type-4 OS=Petunia hybrida GN=PAC1 PE=2 SV=1)

HSP 1 Score: 307.375 bits (786), Expect = 5.393e-83
Identity = 154/187 (82.35%), Postives = 167/187 (89.30%), Query Frame = -2 
Query:  185 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKDDISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMPSGKVKYQVCSPEALSKLLVKHGVTQPAAETS 745
            KMYKIDDHVACAVAGIMSDANILINTARVQAQRYT++YQEPMPVEQLVQSLCDTKQGYTQ+GGL PFGVSFLFAGWDKN+GFQL+MSDPSGNY GWK           QS+LKQDYKDDI+REEAVQLALKVLSKTMDSTSLTS+KLELAEVFL  +GKVKYQ CSPE L+ +LVK G+TQP+AE S
Sbjct:   64 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTFSYQEPMPVEQLVQSLCDTKQGYTQYGGLPPFGVSFLFAGWDKNFGFQLFMSDPSGNYAGWKAAAIGANNQAAQSMLKQDYKDDITREEAVQLALKVLSKTMDSTSLTSEKLELAEVFL-SNGKVKYQACSPEKLNSMLVKSGLTQPSAEES 249
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4B_ORYSJ (Proteasome subunit alpha type-4-2 OS=Oryza sativa subsp. japonica GN=Os06g0167600 PE=2 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 1.164e-77
Identity = 143/186 (76.88%), Postives = 162/186 (87.10%), Query Frame = -2 
Query:  191 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKDDISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMP-SGKVKYQVCSPEALSKLLVKHGVTQPAAE 745
            KMYKID H+ACAVAGIMSDANIL+NTAR+ AQRY  +YQEP+PVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDK++GFQLYMSDPSGNY GWK           QS+LKQDY+D ++REEAV LALKVLSKTMDSTSLT++KLELAEVFL P +G+V+YQVCSPEA+ KLL K G++QPA E
Sbjct:   64 KMYKIDSHLACAVAGIMSDANILLNTARLHAQRYALSYQEPIPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYSGWKAAAVGANSQAAQSMLKQDYRDGLTREEAVALALKVLSKTMDSTSLTAEKLELAEVFLQPGTGEVQYQVCSPEAMGKLLAKAGLSQPAPE 249
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4B_ORYSI (Proteasome subunit alpha type-4-2 OS=Oryza sativa subsp. indica GN=OsI_021067 PE=1 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 1.164e-77
Identity = 143/186 (76.88%), Postives = 162/186 (87.10%), Query Frame = -2 
Query:  191 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKDDISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMP-SGKVKYQVCSPEALSKLLVKHGVTQPAAE 745
            KMYKID H+ACAVAGIMSDANIL+NTAR+ AQRY  +YQEP+PVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDK++GFQLYMSDPSGNY GWK           QS+LKQDY+D ++REEAV LALKVLSKTMDSTSLT++KLELAEVFL P +G+V+YQVCSPEA+ KLL K G++QPA E
Sbjct:   64 KMYKIDSHLACAVAGIMSDANILLNTARLHAQRYALSYQEPIPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYSGWKAAAVGANSQAAQSMLKQDYRDGLTREEAVALALKVLSKTMDSTSLTAEKLELAEVFLQPGTGEVQYQVCSPEAMGKLLAKAGLSQPAPE 249
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4A_ORYSJ (Proteasome subunit alpha type-4-1 OS=Oryza sativa subsp. japonica GN=PAC1 PE=2 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 1.520e-77
Identity = 143/186 (76.88%), Postives = 162/186 (87.10%), Query Frame = -2 
Query:  191 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKDDISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMP-SGKVKYQVCSPEALSKLLVKHGVTQPAAE 745
            KMYKID H+ACAVAGIMSDANIL+NTAR+ AQRY  +YQEP+PVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDK++GFQLYMSDPSGNY GWK           QS+LKQDY+D ++REEAV LALKVLSKTMDSTSLT++KLELAEVFL P +G+V+YQVCSPEA+ KLL K G++QPA E
Sbjct:   64 KMYKIDSHLACAVAGIMSDANILLNTARLHAQRYALSYQEPIPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYSGWKAAAVGANSQAAQSMLKQDYRDGMTREEAVALALKVLSKTMDSTSLTAEKLELAEVFLQPGTGEVQYQVCSPEAMGKLLAKAGLSQPAPE 249
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4A_ORYSI (Proteasome subunit alpha type-4-1 OS=Oryza sativa subsp. indica GN=OsI_021120 PE=1 SV=2)

HSP 1 Score: 289.271 bits (739), Expect = 1.520e-77
Identity = 143/186 (76.88%), Postives = 162/186 (87.10%), Query Frame = -2 
Query:  191 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKDDISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMP-SGKVKYQVCSPEALSKLLVKHGVTQPAAE 745
            KMYKID H+ACAVAGIMSDANIL+NTAR+ AQRY  +YQEP+PVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDK++GFQLYMSDPSGNY GWK           QS+LKQDY+D ++REEAV LALKVLSKTMDSTSLT++KLELAEVFL P +G+V+YQVCSPEA+ KLL K G++QPA E
Sbjct:   64 KMYKIDSHLACAVAGIMSDANILLNTARLHAQRYALSYQEPIPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYSGWKAAAVGANSQAAQSMLKQDYRDGMTREEAVALALKVLSKTMDSTSLTAEKLELAEVFLQPGTGEVQYQVCSPEAMGKLLAKAGLSQPAPE 249
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4_DICDI (Proteasome subunit alpha type-4 OS=Dictyostelium discoideum GN=psmA4 PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 2.361e-54
Identity = 106/182 (58.24%), Postives = 133/182 (73.08%), Query Frame = -2 
Query:  203 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWK-XXXXXXXXXXXQSILKQDYKDDISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMPSGKVKYQVCSPEALSKLLVKHGVTQ 745
            KM+KID+HV CAVAGI SDANILIN AR+ +QR+ Y YQEPMPVEQLV  +CDTKQGYTQ+GGLRPFGVSFL+AGWD++YGFQLY SDPSGN+ GWK            QS+L+ +YK DIS +EA+QLALKVL+KTMD +++ S+KLE +  F      V Y + +   L   + +  + Q
Sbjct:   65 KMFKIDEHVVCAVAGITSDANILINYARLSSQRFFYQYQEPMPVEQLVSQICDTKQGYTQYGGLRPFGVSFLYAGWDRHYGFQLYQSDPSGNFAGWKATSIGGENSQVAQSVLRSNYKPDISLKEALQLALKVLTKTMDRSNINSEKLEFS-YFTKQGDNVVYHIFTAAELDAFIKETDLEQ 245
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4_MOUSE (Proteasome subunit alpha type-4 OS=Mus musculus GN=Psma4 PE=1 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.341e-49
Identity = 98/186 (52.69%), Postives = 131/186 (70.43%), Query Frame = -2 
Query:  191 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKD-DISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMPSGKVKYQVCSPEALSKLLVKHGVTQPAAE 745
            K+YK+++ +AC+VAGI SDAN+L N  R+ AQRY   YQEP+P EQLV +LCD KQ YTQFGG RPFGVS L+ GWDK+YGFQLY SDPSGNYGGWK            S+LKQDYK+ +++ + A+ LA+KVL+KTMD + L+++K+E+A      SGK   +V   + + +L+ KH   +  AE
Sbjct:   64 KIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAVKVLNKTMDVSKLSAEKVEIA-TLTRESGKTVIRVLKQKEVEQLIKKHEEEEAKAE 248
[Close]
BLAST of CF834747 vs. ExPASy Swiss-Prot
Match: PSA4_HUMAN (Proteasome subunit alpha type-4 OS=Homo sapiens GN=PSMA4 PE=1 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 2.287e-49
Identity = 97/186 (52.15%), Postives = 131/186 (70.43%), Query Frame = -2 
Query:  191 KMYKIDDHVACAVAGIMSDANILINTARVQAQRYTYAYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKNYGFQLYMSDPSGNYGGWKXXXXXXXXXXXQSILKQDYKD-DISREEAVQLALKVLSKTMDSTSLTSDKLELAEVFLMPSGKVKYQVCSPEALSKLLVKHGVTQPAAE 745
            K+YK+++ +AC+VAGI SDAN+L N  R+ AQRY   YQEP+P EQLV +LCD KQ YTQFGG RPFGVS L+ GWDK+YGFQLY SDPSGNYGGWK            S+LKQDYK+ +++ + A+ LA+KVL+KTMD + L+++K+E+A      +GK   +V   + + +L+ KH   +  AE
Sbjct:   64 KIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIA-TLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAE 248
The following BLAST results are available for this feature:
BLAST of CF834747 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 156
Match NameE-valueIdentityDescription
PSA4_SPIOL8.039e-8783.96Proteasome subunit alpha type-4 OS=Spinacia olerac... [more]
PSA4_ARATH5.211e-8685.03Proteasome subunit alpha type-4 OS=Arabidopsis tha... [more]
PSA4_PETHY5.393e-8382.35Proteasome subunit alpha type-4 OS=Petunia hybrida... [more]
PSA4B_ORYSJ1.164e-7776.88Proteasome subunit alpha type-4-2 OS=Oryza sativa ... [more]
PSA4B_ORYSI1.164e-7776.88Proteasome subunit alpha type-4-2 OS=Oryza sativa ... [more]
PSA4A_ORYSJ1.520e-7776.88Proteasome subunit alpha type-4-1 OS=Oryza sativa ... [more]
PSA4A_ORYSI1.520e-7776.88Proteasome subunit alpha type-4-1 OS=Oryza sativa ... [more]
PSA4_DICDI2.361e-5458.24Proteasome subunit alpha type-4 OS=Dictyostelium d... [more]
PSA4_MOUSE1.341e-4952.69Proteasome subunit alpha type-4 OS=Mus musculus GN... [more]
PSA4_HUMAN2.287e-4952.15Proteasome subunit alpha type-4 OS=Homo sapiens GN... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06A21_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06A21, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834747 ID=CF834747; Name=CF834747; organism=Citrus sinensis; type=EST; length=746bp
TTTTTTTTTTTTTTTAGGTAAACTGCATCCAACATCATAACATCTGCCAT
TACCAAGCTTCTGATCATCAACATCAAACACATCGATCTGCAGTTCTTTG
GGCAAAAAGCATAAAAGTAATACAAGTACTGTCCAAATTGCTTTGAAATT
AAGTTATAATGAAAGCATGGAAGATCTTCATTCAAGAAGTCTCAGCAGCA
GGTTGCGTTACTCCATGCTTCACCAACAACTTGCTCAGAGCTTCGGGTGA
GCAAACTTGGTACTTCACTTTGCCAGAAGGCATCAGGAAAACCTCAGCTA
ATTCAAGCTTATCAGAAGTAAGACTGGTGCTGTCCATGGTTTTGCTGAGC
ACCTTCAGTGCTAGTTGAACTGCTTCTTCCCTTGAAATATCATCCTTGTA
ATCTTGTTTGAGGATTGATTGTGCAGCCTGGTTATTTGCACCAATAGCAG
CAGCTTTCCAACCACCATAGTTTCCACTAGGATCACTCATGTAAAGTTGG
AAGCCATAATTTTTGTCCCACCCTGCAAAAAGAAATGAAACACCAAACGG
GCGGAGACCGCCAAACTGGGTGTAACCTTGCTTTGTATCACAAAGAGATT
GAACAAGCTGCTCAACAGGCATTGGCTCTTGGTAGGCATATGTGTAACGT
TGGGCTTGGACCCTGGCAGTGTTGATTAGGATGTTCGCATCAGACATTAT
TCCAGCTACGGCACAAGCAACGTGATCATCAATCTTGTACATCTTC
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