CF834867 | Citrus Genome Database

Overview


Name	CF834867
Unique Name	CF834867
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	594

Libraries


Library Name	Type
Ruby Orange Ovary at Anthesis cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2387	contig	Csv1_Contig2387:467..1060.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_ACTCH (Pyrroline-5-carboxylate reductase OS=Actinidia chinensis PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.720e-54
Identity = 67/89 (75.28%), Postives = 69/89 (77.53%), Query Frame = -3

Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAYIF              LPRELALGLASQTVLGAASMV + GKHPGQLKDDVAS GGTTIAGIHELEK GFRG LMNAVV+A
Sbjct:  180 LSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVARGGKHPGQLKDDVASAGGTTIAGIHELEKGGFRGTLMNAVVSA 268

HSP 2 Score: 108.227 bits (269), Expect = 1.720e-54
Identity = 51/60 (85.00%), Postives = 57/60 (95.00%), Query Frame = -2

Query:  414 HSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 593
            HSRFIRVMPNTP+AVGEAA+VMSLG TAT EDGELI KLFG++GKIW+ADEKLFDA+TGL
Sbjct:  121 HSRFIRVMPNTPAAVGEAASVMSLGATATGEDGELITKLFGAIGKIWKADEKLFDAVTGL 180

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_PEA (Pyrroline-5-carboxylate reductase OS=Pisum sativum GN=PROC PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.197e-52
Identity = 66/90 (73.33%), Postives = 69/90 (76.67%), Query Frame = -3

Query:  134 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 403
            L GSGPAYI+              LPR+LAL LASQTVLGAASM T SGKHPGQLKDDV SPGGTTIAG+HELEK GFRG LMNAVVAAA
Sbjct:  177 LSGSGPAYIYLAIEALADGGVAAGLPRDLALSLASQTVLGAASMATLSGKHPGQLKDDVTSPGGTTIAGVHELEKGGFRGTLMNAVVAAA 266

HSP 2 Score: 102.449 bits (254), Expect = 1.197e-52
Identity = 47/60 (78.33%), Postives = 54/60 (90.00%), Query Frame = -2

Query:  414 HSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 593
            H RFIRVMPNTP+AVG+AA+VMSLGG ATEED  LI +LFGS+GKIW+AD+K FDAITGL
Sbjct:  118 HERFIRVMPNTPAAVGQAASVMSLGGAATEEDANLISQLFGSIGKIWKADDKFFDAITGL 177

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_SOYBN (Pyrroline-5-carboxylate reductase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 8.317e-51
Identity = 62/90 (68.89%), Postives = 69/90 (76.67%), Query Frame = -3

Query:  134 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 403
            L GSGPAY++              LPR+L+L LASQTVLGAASMV+++GKHPGQLKDDV SPGGTTI GIHELE  GFRG LMNAVVAAA
Sbjct:  178 LSGSGPAYVYLAIEALADGGVAAGLPRDLSLSLASQTVLGAASMVSQTGKHPGQLKDDVTSPGGTTITGIHELENGGFRGTLMNAVVAAA 267

HSP 2 Score: 100.908 bits (250), Expect = 8.317e-51
Identity = 46/60 (76.67%), Postives = 56/60 (93.33%), Query Frame = -2

Query:  414 HSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 593
            + RFIRVMPNTP+AVG+AA+VMSLGG+ATEEDG +I +LFGS+GKIW+A+EK FDAITGL
Sbjct:  119 NDRFIRVMPNTPAAVGQAASVMSLGGSATEEDGNIIAQLFGSIGKIWKAEEKYFDAITGL 178

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR1_ARATH (Pyrroline-5-carboxylate reductase OS=Arabidopsis thaliana GN=PROC1 PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 2.831e-48
Identity = 64/90 (71.11%), Postives = 68/90 (75.56%), Query Frame = -3

Query:  134 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 403
            L GSGPAYIF              LPRELAL LASQTVLGAA+MV+K+GKHPG LKDDV SPGGTTIAG+HELEK  FR  LMNAVVAAA
Sbjct:  178 LSGSGPAYIFLAIEALADGGVAAGLPRELALSLASQTVLGAATMVSKTGKHPGVLKDDVTSPGGTTIAGVHELEKGSFRATLMNAVVAAA 267

HSP 2 Score: 92.4337 bits (228), Expect = 2.831e-48
Identity = 44/58 (75.86%), Postives = 52/58 (89.66%), Query Frame = -2

Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            RFIRVMPNTP+AVGEAA+VMSLG  ATEEDG ++  LFG+VGKI +ADEK+FDA+TGL
Sbjct:  121 RFIRVMPNTPAAVGEAASVMSLGTGATEEDGAIVAMLFGAVGKILKADEKMFDAVTGL 178

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR1_PONAB (Pyrroline-5-carboxylate reductase 1, mitochondrial OS=Pongo abelii GN=PYCR1 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.427e-26
Identity = 46/89 (51.69%), Postives = 57/89 (64.04%), Query Frame = -3

Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              LPR LA+ L +Q +LGAA M+  S +HPGQLKD+V+SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEAS 261

HSP 2 Score: 52.7582 bits (125), Expect = 1.427e-26
Identity = 29/58 (50.00%), Postives = 34/58 (58.62%), Query Frame = -2

Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            R IR M NTP  V E ATV + G  A  EDG L+ +L  SVG     +E L DA+TGL
Sbjct:  116 RVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGL 173

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR1_HUMAN (Pyrroline-5-carboxylate reductase 1, mitochondrial OS=Homo sapiens GN=PYCR1 PE=1 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 1.427e-26
Identity = 46/89 (51.69%), Postives = 57/89 (64.04%), Query Frame = -3

Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              LPR LA+ L +Q +LGAA M+  S +HPGQLKD+V+SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEAS 261

HSP 2 Score: 52.7582 bits (125), Expect = 1.427e-26
Identity = 29/58 (50.00%), Postives = 34/58 (58.62%), Query Frame = -2

Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            R IR M NTP  V E ATV + G  A  EDG L+ +L  SVG     +E L DA+TGL
Sbjct:  116 RVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGL 173

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR2_BOVIN (Pyrroline-5-carboxylate reductase 2 OS=Bos taurus GN=PYCR2 PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.147e-26
Identity = 46/89 (51.69%), Postives = 56/89 (62.92%), Query Frame = -3

Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              LPR LA+ L +Q +LGAA M+  S +HPGQLKD+V SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLDSEQHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEAS 261

HSP 2 Score: 52.373 bits (124), Expect = 3.147e-26
Identity = 28/58 (48.28%), Postives = 35/58 (60.34%), Query Frame = -2

Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR M NTP  V E ATV + G  A  EDG+L+ +L  SVG     +E L DA+TGL
Sbjct:  116 KVIRCMTNTPVLVREGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGL 173

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR2_RAT (Pyrroline-5-carboxylate reductase 2 OS=Rattus norvegicus GN=Pycr2 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 4.097e-26
Identity = 45/89 (50.56%), Postives = 55/89 (61.80%), Query Frame = -3

Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              +PR LA+ L +Q +LGAA M+  S  HPGQLKD+V SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQALLGAAKMLLDSEDHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEAS 261

HSP 2 Score: 52.7582 bits (125), Expect = 4.097e-26
Identity = 29/58 (50.00%), Postives = 35/58 (60.34%), Query Frame = -2

Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR M NTP  V E ATV + G  A  EDG+L+ +L  SVG     +E L DAITGL
Sbjct:  116 KVIRCMTNTPVVVREGATVYATGTHALVEDGKLLEQLMSSVGFCTEVEEDLIDAITGL 173

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR2_MOUSE (Pyrroline-5-carboxylate reductase 2 OS=Mus musculus GN=Pycr2 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 4.097e-26
Identity = 45/89 (50.56%), Postives = 55/89 (61.80%), Query Frame = -3

Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              +PR LA+ L +Q +LGAA M+  S  HPGQLKD+V SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQALLGAAKMLLDSEDHPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEAS 261

HSP 2 Score: 52.7582 bits (125), Expect = 4.097e-26
Identity = 29/58 (50.00%), Postives = 35/58 (60.34%), Query Frame = -2

Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR M NTP  V E ATV + G  A  EDG+L+ +L  SVG     +E L DAITGL
Sbjct:  116 KVIRCMTNTPVVVREGATVYATGTHALVEDGKLLEQLMSSVGFCTEVEEDLIDAITGL 173

[Close]

BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_METAC (Pyrroline-5-carboxylate reductase OS=Methanosarcina acetivorans GN=proC PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 5.352e-26
Identity = 47/90 (52.22%), Postives = 58/90 (64.44%), Query Frame = -3

Query:  134 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 403
            L GSGPA+IF              + R+ AL LA+QTVLGAA M  ++G HPG+LKD V SP GTTI GIH LE++G R   MNAV+ A+
Sbjct:  172 LSGSGPAFIFPVIEAMADGAVLEGMDRKSALTLAAQTVLGAAKMALETGMHPGELKDMVTSPAGTTIQGIHSLEEAGIRAAFMNAVIRAS 261

HSP 2 Score: 48.521 bits (114), Expect = 5.352e-26
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = -2

Query:  414 SRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 590
            +R +RVMPN  + V EAA+ ++ G  AT ED +   ++F +VG   +  E L DA+TGL
Sbjct:  114 TRVVRVMPNIAATVSEAASGIAPGKNATPEDLKAALEIFSAVGTAVQVPESLMDAVTGL 172

The following BLAST results are available for this feature:

BLAST of CF834867 vs. ExPASy Swiss-Prot

BLAST of CF834867 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 35


Match Name	E-value	Identity	Description
P5CR_ACTCH	1.720e-54	75.28	Pyrroline-5-carboxylate reductase OS=Actinidia chi...	[more]
P5CR_PEA	1.197e-52	73.33	Pyrroline-5-carboxylate reductase OS=Pisum sativum...	[more]
P5CR_SOYBN	8.317e-51	68.89	Pyrroline-5-carboxylate reductase OS=Glycine max P...	[more]
P5CR1_ARATH	2.831e-48	71.11	Pyrroline-5-carboxylate reductase OS=Arabidopsis t...	[more]
P5CR1_PONAB	1.427e-26	51.69	Pyrroline-5-carboxylate reductase 1, mitochondrial...	[more]
P5CR1_HUMAN	1.427e-26	51.69	Pyrroline-5-carboxylate reductase 1, mitochondrial...	[more]
P5CR2_BOVIN	3.147e-26	51.69	Pyrroline-5-carboxylate reductase 2 OS=Bos taurus ...	[more]
P5CR2_RAT	4.097e-26	50.56	Pyrroline-5-carboxylate reductase 2 OS=Rattus norv...	[more]
P5CR2_MOUSE	4.097e-26	50.56	Pyrroline-5-carboxylate reductase 2 OS=Mus musculu...	[more]
P5CR_METAC	5.352e-26	52.22	Pyrroline-5-carboxylate reductase OS=Methanosarcin...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS02_06E05_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06E05, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF834867 ID=CF834867; Name=CF834867; organism=Citrus sinensis; type=EST; length=594bp
ATGCAACTGCCCCTTTCAATATCCATACAGAAAACAAAAAAAAGGAGAGG
GTTAATCGCCATACTAGAAGTGGAAGAGCTGAGAAACCGGAATCAAAATG
AATTAGCTCTAGGACAGTTCTCGGCTTCGCGTGAGCAGCAGCAACAACAG
CATTCATTAATATCCCACGAAATCCACTTTTCTCCAACTCATGAATACCA
GCAATAGTAGTCCCACCAGGCGATGCAACATCATCTTTTAGCTGACCAGG
ATGCTTCCCACTTTTAGTGACCATTGATGCTGCTCCTAATACTGTCTGAG
AAGCTAGACCAAGTGCAAGTTCTCGCGGTAGCCCTGCAGCCACAGCTGCA
TCAGCCAAAGCTTCAATTGCTAAAAATATATATGCTGGACCGCTGCCAAG
AAGCCACATACCTCAGGCCCGTGATTGCATCAAACAATTTCTCATCCGCT
CGCCATATCTTGCCAACTGATCCGAATAATTTACCGATTAGTTCCCCATC
CTCTTCTGTTGCAGTCCCTCCCAAGCTCATAACTGTTGCAGCCTCACCTA
CAGCAGAAGGTGTATTAGGCATAACTCTAATAAACCGGCTGTGA