CF835163

Overview
NameCF835163
Unique NameCF835163
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length657
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835163_ssr113 microsatellite CF835163_ssr113:113..128. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947 contig Csv1_Contig10947:93..750. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_LITCN (S-adenosylmethionine synthetase OS=Litchi chinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 2.993e-68
Identity = 127/145 (87.59%), Postives = 133/145 (91.72%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EILKIVKENFDFRPGMIS+NLDLKRGGNGRFLKTAAYGHFGR+DPDFTWE+VKPLKW+K+QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILKIVKENFDFRPGMISVNLDLKRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWDKVQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_VITVI (S-adenosylmethionine synthetase 2 OS=Vitis vinifera GN=METK2 PE=3 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 2.993e-68
Identity = 128/145 (88.28%), Postives = 132/145 (91.03%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKWEK QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKTQA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_SOLTU (S-adenosylmethionine synthetase 1 OS=Solanum tuberosum GN=METK1 PE=2 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 6.667e-68
Identity = 128/144 (88.89%), Postives = 131/144 (90.97%), Query Frame = -3 
Query:  224 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQ 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKENFDFRPGM+SINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKWEK Q
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMMSINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 392
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK5_VITVI (S-adenosylmethionine synthetase 5 OS=Vitis vinifera GN=METK5 PE=3 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 1.137e-67
Identity = 127/142 (89.44%), Postives = 130/142 (91.55%), Query Frame = -3 
Query:  230 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEK 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMI+INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKWEK
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMIAINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 390
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_ELAUM (S-adenosylmethionine synthetase 2 OS=Elaeagnus umbellata GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 5.644e-67
Identity = 127/145 (87.59%), Postives = 131/145 (90.34%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVP+PLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMI+INLDLKRGGN RFLKTAAYGHFGRDDPDFTWEIVKPLKWEK Q+
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPDPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMITINLDLKRGGNDRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKPQS 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_MEDTR (S-adenosylmethionine synthetase OS=Medicago truncatula GN=METK PE=3 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 9.628e-67
Identity = 125/145 (86.21%), Postives = 131/145 (90.34%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAY+VRQAAKSIVA+GLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL+IVKENFDFRPGMI+INLDLKRGGN RFLKTAAYGHFGRDDPDFTWE+VKPLKWEK QA
Sbjct:  250 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYVVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKENFDFRPGMITINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 394
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_CATRO (S-adenosylmethionine synthetase 1 OS=Catharanthus roseus GN=SAMS1 PE=1 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 9.628e-67
Identity = 125/142 (88.03%), Postives = 130/142 (91.55%), Query Frame = -3 
Query:  230 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEK 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKENFDFRPGMI+INLDLKRGG+GRFLKTAAYGHFGRDDPDFTWE+VKPLKWEK
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMIAINLDLKRGGSGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 390
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_SOLLC (S-adenosylmethionine synthetase 1 OS=Solanum lycopersicum GN=SAM1 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.257e-66
Identity = 126/144 (87.50%), Postives = 130/144 (90.28%), Query Frame = -3 
Query:  224 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQ 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKENFDFRPGM+SINLDLKRGGN RFLKTAAYGHFGRDDPDFTWE+VKPLKWEK Q
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKENFDFRPGMMSINLDLKRGGNRRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 392
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_PETCR (S-adenosylmethionine synthetase 1 (Fragment) OS=Petroselinum crispum GN=SMS-1 PE=2 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 1.642e-66
Identity = 125/142 (88.03%), Postives = 129/142 (90.85%), Query Frame = -3 
Query:  230 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEK 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKE FDFRPGMISINLDLKRGGNGRFLKTAAYGHFGR+DPDFTWE+VKPLKWEK
Sbjct:   92 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 233
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_PETCR (S-adenosylmethionine synthetase 2 (Fragment) OS=Petroselinum crispum GN=SMS-2 PE=2 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 2.145e-66
Identity = 125/142 (88.03%), Postives = 129/142 (90.85%), Query Frame = -3 
Query:  230 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEK 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL+IVKE FDFRPGMISINLDLKRGGNGRFLKTAAYGHFGR+DPDFTWE+VKPLKWEK
Sbjct:    3 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 144
The following BLAST results are available for this feature:
BLAST of CF835163 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_LITCN2.993e-6887.59S-adenosylmethionine synthetase OS=Litchi chinensi... [more]
METK2_VITVI2.993e-6888.28S-adenosylmethionine synthetase 2 OS=Vitis vinifer... [more]
METK1_SOLTU6.667e-6888.89S-adenosylmethionine synthetase 1 OS=Solanum tuber... [more]
METK5_VITVI1.137e-6789.44S-adenosylmethionine synthetase 5 OS=Vitis vinifer... [more]
METK2_ELAUM5.644e-6787.59S-adenosylmethionine synthetase 2 OS=Elaeagnus umb... [more]
METK_MEDTR9.628e-6786.21S-adenosylmethionine synthetase OS=Medicago trunca... [more]
METK1_CATRO9.628e-6788.03S-adenosylmethionine synthetase 1 OS=Catharanthus ... [more]
METK1_SOLLC1.257e-6687.50S-adenosylmethionine synthetase 1 OS=Solanum lycop... [more]
METK1_PETCR1.642e-6688.03S-adenosylmethionine synthetase 1 (Fragment) OS=Pe... [more]
METK2_PETCR2.145e-6688.03S-adenosylmethionine synthetase 2 (Fragment) OS=Pe... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835163 ID=CF835163; Name=CF835163; organism=Citrus sinensis; type=EST; length=657bp
TTGGTTTACCCTTAAGAGAATTTCAGAACAATTCATATTCGCCTCTCCCC
CCATTATGCCTTGGATTGAGAATCCATTAAACATATTTATTAAAAAAAAA
GGGGAAAAGAAGAAGAAAGAAAGAAAGAAAACATTCATGTAACTGAAACA
GAAGTAAAAAAGAAAAGTAGACATATATAAGCACCAATGAGAGGCCAGCA
GCTCATCCTTGATTAGTTTAAGCTTGAAGTTTCTCCCACTTGAGTGGCTT
AACAATCTCCCAAGTGAAATCAGGATCATCTCTGCCAAAGTGCCCATAAG
CGGCAGTCTTCAAGAACCTGCCGTTGCCACCCCTCTTGAGATCAAGATTA
ATAGAAATCATCCCAGGCCTAAAATCAAAATTCTCCTTCACAATCTTCAA
AATTTCCTTGTCAGGGATCTTCCCAGTCCCATAACTATCAACAAAAACTG
ACAAAGGCTCGGGCACACCAATAGCGTAAGAAACCTGAACAATGCATCTC
CTGGCAAGCCCGCTTGCCACAATACTCTTAGCAGCTTGCCTTACAATGTA
AGCACCGCTTCTATCAACCTTGGTAGGATCTTTCCCTGAGAAAGCCCCAC
CACCGTGTGCCCCCCAGCCACCATAAGTATCAATAATAATCTTCCGGCCG
GTGAGCC
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