CF835164

Overview
NameCF835164
Unique NameCF835164
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length548
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835164_ssr33 microsatellite CF835164_ssr33:33..42. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig12594 contig Csv1_Contig12594:11..558. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_SUASA (S-adenosylmethionine synthetase 2 OS=Suaeda salsa GN=METK2 PE=2 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 1.089e-61
Identity = 112/119 (94.12%), Postives = 117/119 (98.32%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ME+FLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKAN+DYEKIVRDTCRSIGF SADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTKRPE++G
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANIDYEKIVRDTCRSIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEDVG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK_LITCN (S-adenosylmethionine synthetase OS=Litchi chinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 1.858e-61
Identity = 113/119 (94.96%), Postives = 117/119 (98.32%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCT+TNM MVFGEITTKANVDYE+IVRDTCRSIGFTS DVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTRTNMVMVFGEITTKANVDYEQIVRDTCRSIGFTSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_TOBAC (S-adenosylmethionine synthetase 2 OS=Nicotiana tabacum GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 1.858e-61
Identity = 112/119 (94.12%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR IGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRGIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_PETHY (S-adenosylmethionine synthetase 1 OS=Petunia hybrida GN=SAM1 PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 1.858e-61
Identity = 112/119 (94.12%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR IGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_BETVU (S-adenosylmethionine synthetase 2 OS=Beta vulgaris GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 3.170e-61
Identity = 114/119 (95.80%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDP+SKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCRSIGFTS DVGLDAD CKVLVNIEQQSPDIAQGVHGHLTKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPESKVACETCTKTNMVMVFGEITTKAEVDYEKIVRDTCRSIGFTSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_ORYSJ (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. japonica GN=SAM1 PE=2 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 3.170e-61
Identity = 111/119 (93.28%), Postives = 116/119 (97.48%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ++TFLFTSESVNEGHPDKLCDQVSDAVLDACLA+DPDSKVACETCTKTNM MVFGEITTKANVDYEKIVR+TCR+IGF SADVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    4 LDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 122
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_ORYSI (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. indica GN=SAM1 PE=2 SV=2)

HSP 1 Score: 234.187 bits (596), Expect = 3.170e-61
Identity = 111/119 (93.28%), Postives = 116/119 (97.48%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ++TFLFTSESVNEGHPDKLCDQVSDAVLDACLA+DPDSKVACETCTKTNM MVFGEITTKANVDYEKIVR+TCR+IGF SADVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    4 LDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 122
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK4_POPTR (S-adenosylmethionine synthetase 4 OS=Populus trichocarpa GN=METK4 PE=2 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 4.140e-61
Identity = 111/119 (93.28%), Postives = 117/119 (98.32%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            M+TFLFTSESVNEGHPDKLCDQVSDA+LDACLAQDP+SKVACETC+KTNM MVFGEITTKANVDYEKIVRDTCR IGFTSADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLAQDPESKVACETCSKTNMVMVFGEITTKANVDYEKIVRDTCRGIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK4_HORVU (S-adenosylmethionine synthetase 4 OS=Hordeum vulgare GN=SAM4 PE=2 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 5.407e-61
Identity = 111/119 (93.28%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            +ETFLFTSESVNEGHPDKLCDQ+SDAVLDACLA+DPDSKVACETCTKTNM MVFGEITTKANVDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    4 VETFLFTSESVNEGHPDKLCDQISDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRGIGFVSNDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 122
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK5_VITVI (S-adenosylmethionine synthetase 5 OS=Vitis vinifera GN=METK5 PE=3 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.061e-61
Identity = 111/119 (93.28%), Postives = 116/119 (97.48%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKA++DYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADIDYEKIVRDTCRTIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 119
The following BLAST results are available for this feature:
BLAST of CF835164 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK2_SUASA1.089e-6194.12S-adenosylmethionine synthetase 2 OS=Suaeda salsa ... [more]
METK_LITCN1.858e-6194.96S-adenosylmethionine synthetase OS=Litchi chinensi... [more]
METK2_TOBAC1.858e-6194.12S-adenosylmethionine synthetase 2 OS=Nicotiana tab... [more]
METK1_PETHY1.858e-6194.12S-adenosylmethionine synthetase 1 OS=Petunia hybri... [more]
METK2_BETVU3.170e-6195.80S-adenosylmethionine synthetase 2 OS=Beta vulgaris... [more]
METK1_ORYSJ3.170e-6193.28S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK1_ORYSI3.170e-6193.28S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK4_POPTR4.140e-6193.28S-adenosylmethionine synthetase 4 OS=Populus trich... [more]
METK4_HORVU5.407e-6193.28S-adenosylmethionine synthetase 4 OS=Hordeum vulga... [more]
METK5_VITVI7.061e-6193.28S-adenosylmethionine synthetase 5 OS=Vitis vinifer... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835164 ID=CF835164; Name=CF835164; organism=Citrus sinensis; type=EST; length=548bp
GAAACTACAAATCCTTGCTGCCGTTTTACCTCCTCTCTCTCTTCTCTCAG
CGTATGCATATCGGCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGT
ATATCACCACCTTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAA
GCGAAAGCTGCAGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATT
TCTTTTCACATCAGAATCAGTGAACGAGGGTCACCCGGACAAGCTGTGTG
ACCAAGTTTCGGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGAC
AGCAAAGTTGCTTGTGAAACATGCACAAAGACCAACATGGACATGGTTTT
TGGAGAAATTACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTG
ATACATGCAGATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT
GATCACTGCAAAGTTCTTGTTAATATTGAGCAGCAAAGCCCTGACATTGC
TCAGGGTGTTCATGGGCATCTCACAAAGCGGCCTGAGGAGATTGGTGC
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