CF835216

Overview
NameCF835216
Unique NameCF835216
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length504
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2871 contig Csv1_Contig2871:680..1183. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PALY_VITVI (Phenylalanine ammonia-lyase (Fragment) OS=Vitis vinifera GN=PAL PE=2 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 9.043e-46
Identity = 85/110 (77.27%), Postives = 98/110 (89.09%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            LNNGE+EK  ++SIFQKI AFEEELK VLPKEVE+AR  VE+G+P+IPNRIKECRSYPLY+ VREELG+  LTGEKV SPGE+FDKVFTAMC+GKIIDP+L+CL  WNGA
Sbjct:  302 LNNGESEKNGSTSIFQKIGAFEEELKAVLPKEVESARDGVESGNPSIPNRIKECRSYPLYKFVREELGTGLLTGEKVRSPGEDFDKVFTAMCEGKIIDPLLDCLSAWNGA 411
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PAL3_TOBAC (Phenylalanine ammonia-lyase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 7.655e-45
Identity = 87/110 (79.09%), Postives = 95/110 (86.36%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            L NGENEK ANSSIFQKI AFE ELK VLPKEVE+AR ++ENG+P I NRIKECRSYPLYR VREELG+  LTGEKV SPGEE DKVFTAMC G+IID +LECL+EWNGA
Sbjct:  598 LENGENEKNANSSIFQKILAFEGELKAVLPKEVESARISLENGNPAIANRIKECRSYPLYRFVREELGAELLTGEKVRSPGEECDKVFTAMCNGQIIDSLLECLKEWNGA 707
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PAL1_TOBAC (Phenylalanine ammonia-lyase OS=Nicotiana tabacum GN=TPA1 PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 7.655e-45
Identity = 84/110 (76.36%), Postives = 95/110 (86.36%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            +NNGE+EK  NSSIFQKI AFE+ELK VLPKEVE+AR  +E+G+P IPNRI ECRSYPLYR VR+ELG+  LTGEKV SPGEE DKVFTAMC G+IIDPMLECL+ WNGA
Sbjct:  601 MNNGESEKNVNSSIFQKIGAFEDELKAVLPKEVESARAALESGNPAIPNRITECRSYPLYRFVRKELGTELLTGEKVRSPGEECDKVFTAMCNGQIIDPMLECLKSWNGA 710
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PAL1_ARATH (Phenylalanine ammonia-lyase 1 OS=Arabidopsis thaliana GN=PAL1 PE=1 SV=3)

HSP 1 Score: 179.104 bits (453), Expect = 9.998e-45
Identity = 86/110 (78.18%), Postives = 94/110 (85.45%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            L NGE+EK A +SIF KI AFEEELK VLPKEVE AR   +NG+  IPNRIKECRSYPLYR VREELG+  LTGEKVTSPGEEFDKVFTA+C+GKIIDPM+ECL EWNGA
Sbjct:  611 LINGESEKNAVTSIFHKIGAFEEELKAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELLTGEKVTSPGEEFDKVFTAICEGKIIDPMMECLNEWNGA 720
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PALY_CITLI (Phenylalanine ammonia-lyase OS=Citrus limon GN=PAL6 PE=2 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 2.227e-44
Identity = 81/110 (73.64%), Postives = 99/110 (90.00%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            L+NG+ EK + +SIFQKI AFE+ELKT+LPKEVE AR  +E+G+  IPNRIKECRSYPLY++VRE++G++ LTGEKV SPGEEFDKVFTAMC+GK+IDPMLECL+EWNGA
Sbjct:  606 LDNGDREKNSTTSIFQKIGAFEDELKTLLPKEVEIARTELESGNAAIPNRIKECRSYPLYKIVREDIGTSLLTGEKVRSPGEEFDKVFTAMCEGKLIDPMLECLKEWNGA 715
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PAL2_ARATH (Phenylalanine ammonia-lyase 2 OS=Arabidopsis thaliana GN=PAL2 PE=1 SV=2)

HSP 1 Score: 177.563 bits (449), Expect = 2.909e-44
Identity = 84/110 (76.36%), Postives = 94/110 (85.45%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            L+NGE EK A +SIFQKI AFEEELK VLPKEVE AR    NG+  IPNRIKECRSYPLYR VREELG+  LTGEKV SPGEEFDKVFTAMC+GK+IDP+++CL+EWNGA
Sbjct:  603 LSNGETEKNAVTSIFQKIGAFEEELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVSPGEEFDKVFTAMCEGKLIDPLMDCLKEWNGA 712
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PAL2_TOBAC (Phenylalanine ammonia-lyase OS=Nicotiana tabacum GN=PALA PE=2 SV=2)

HSP 1 Score: 176.792 bits (447), Expect = 4.962e-44
Identity = 85/110 (77.27%), Postives = 94/110 (85.45%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            L NGENEK ANSSIFQKI AFE+ELK VLPKEVE+AR  +E+G+P I NRIKECRSYPLYR VR ELG+  LTGEKV SPGEE DKVFTAMC G+IID +LECL+EWNGA
Sbjct:  598 LQNGENEKNANSSIFQKILAFEDELKAVLPKEVESARAALESGNPAIANRIKECRSYPLYRFVRGELGAELLTGEKVRSPGEECDKVFTAMCNGQIIDSLLECLKEWNGA 707
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PAL1_POPKI (Phenylalanine ammonia-lyase G1 OS=Populus kitakamiensis GN=PALG1 PE=3 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 4.962e-44
Identity = 81/110 (73.64%), Postives = 97/110 (88.18%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            L NGE E  + +SIFQKI +FEEELKT+LPKEVE+AR  VENG+P +PNRIKECRSYPLY+ VREELG++ LTGEKV SPGE+FDKVFTA+C GK++DP+LECL+EWNGA
Sbjct:  568 LVNGERETNSTTSIFQKIRSFEEELKTLLPKEVESARLEVENGNPVVPNRIKECRSYPLYKFVREELGTSLLTGEKVKSPGEDFDKVFTAICAGKLMDPLLECLKEWNGA 677
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PAL1_LITER (Phenylalanine ammonia-lyase 1 OS=Lithospermum erythrorhizon PE=2 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 4.962e-44
Identity = 82/110 (74.55%), Postives = 93/110 (84.55%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            L+NGENEK  N+SIF KIA FEEELK +LPKEVENAR +VENG P I NRI+ECRSYPLY+ VREELG+  LTGEKV SPGEE DKVFTAMC+GK++DP+L CL  WNGA
Sbjct:  596 LDNGENEKDVNTSIFHKIAIFEEELKAILPKEVENARASVENGIPAISNRIEECRSYPLYKFVREELGTELLTGEKVRSPGEELDKVFTAMCEGKLVDPLLACLEAWNGA 705
[Close]
BLAST of CF835216 vs. ExPASy Swiss-Prot
Match: PAL5_SOLLC (Phenylalanine ammonia-lyase OS=Solanum lycopersicum GN=PAL5 PE=3 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 6.481e-44
Identity = 82/110 (74.55%), Postives = 94/110 (85.45%), Query Frame = -2 
Query:  174 LNNGENEKTANSSIFQKIAAFEEELKTVLPKEVENARQTVENGSPTIPNRIKECRSYPLYRLVREELGSNFLTGEKVTSPGEEFDKVFTAMCQGKIIDPMLECLREWNGA 503
            + NGE+EK  NSSIFQKI AFE+ELK VLPKEVE+AR  VE+G+P IPNRI ECRSYPLYRLVR+E+G+  LTGEKV SPGEE DKVFTA C G+IIDP+LECL+ WNGA
Sbjct:  607 MKNGESEKNLNSSIFQKIVAFEDELKAVLPKEVESARAVVESGNPAIPNRITECRSYPLYRLVRQEVGTELLTGEKVRSPGEEIDKVFTAFCNGQIIDPLLECLKSWNGA 716
The following BLAST results are available for this feature:
BLAST of CF835216 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 47
Match NameE-valueIdentityDescription
PALY_VITVI9.043e-4677.27Phenylalanine ammonia-lyase (Fragment) OS=Vitis vi... [more]
PAL3_TOBAC7.655e-4579.09Phenylalanine ammonia-lyase OS=Nicotiana tabacum P... [more]
PAL1_TOBAC7.655e-4576.36Phenylalanine ammonia-lyase OS=Nicotiana tabacum G... [more]
PAL1_ARATH9.998e-4578.18Phenylalanine ammonia-lyase 1 OS=Arabidopsis thali... [more]
PALY_CITLI2.227e-4473.64Phenylalanine ammonia-lyase OS=Citrus limon GN=PAL... [more]
PAL2_ARATH2.909e-4476.36Phenylalanine ammonia-lyase 2 OS=Arabidopsis thali... [more]
PAL2_TOBAC4.962e-4477.27Phenylalanine ammonia-lyase OS=Nicotiana tabacum G... [more]
PAL1_POPKI4.962e-4473.64Phenylalanine ammonia-lyase G1 OS=Populus kitakami... [more]
PAL1_LITER4.962e-4474.55Phenylalanine ammonia-lyase 1 OS=Lithospermum eryt... [more]
PAL5_SOLLC6.481e-4474.55Phenylalanine ammonia-lyase OS=Solanum lycopersicu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06N18_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06N18, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835216 ID=CF835216; Name=CF835216; organism=Citrus sinensis; type=EST; length=504bp
AAAGGACAATAAGTCTTATAACCTTATATGAAAAAACCAATAGAAGTGAT
TGAAATATTACAGATGAATTTGTACCACTGACTCGCATGGGCAAATTCTA
TGAACACAAGAACACAAGGGATTGAAGACCGTGTTATTAAACAAAATAAA
AACAACTAACAAATTGGAAGAGGGGCGCCATTCCATTCCCTGAGACATTC
CAGCATTGGATCAATGATCTTGCCCTGGCACATTGCTGTAAACACTTTGT
CAAATTCCTCGCCGGGCGATGTAACTTTTTCCCCTGTTAGAAAATTAGAC
CCGAGCTCCTCCCTCACTAACCTGTACAAAGGGTAAGACCTGCATTCTTT
GATCCTGTTGGGAATTGTTGGACTTCCATTCTCAACAGTCTGCCTGGCGT
TCTCAACTTCTTTGGGCAAAACGGTTTTCAATTCCTCCTCAAAGGCTGCA
ATCTTCTGGAAGATTGAAGAGTTGGCAGTCTTCTCGTTCTCTCCATTGTT
CAGT
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