CF835333

Overview
NameCF835333
Unique NameCF835333
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length793
Libraries
Library NameType
Washington Navel Orange Shoot Meristem cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1296 contig Csv1_Contig1296:730..1522. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CF835333 vs. ExPASy Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 9.048e-31
Identity = 74/199 (37.19%), Postives = 113/199 (56.78%), Query Frame = 2
Query:   29 ATTLPFLILALASNSLLVLGTSSVPEPLLDVHGNKVESTIKYYIVSAIWXXXXXXXXXXXXXXXYCPLDVIQLPSDMQNGIELTLSPYNNST-IVRESTDLNLRFSVLLSGRD-YCNEQPLWKVDNYDAASGKWFITTGGLDGHPGAETLLNWFKLEKIGNFPGIYKIVHCPSVCESCVKLCHNVGR-SFEDGVRRLVL 616
            AT +  L+ A  S S      ++   P+LD  G+++++ ++YY++S+I                 CP  V+Q  SD+ NG  +  S  ++   +VR STD+N+ F  +   RD  C+   +W++DNYD ++GKW++TT G+ G PG  TL +WFK+EK G     YK   CPSVC+SC  LC ++GR S +DG  RL L
Sbjct:    4 ATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPI---RDRLCSTSTVWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLG--YKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLAL 197          
BLAST of CF835333 vs. ExPASy Swiss-Prot
Match: MIRA_RICDU (Miraculin OS=Richadella dulcifica PE=1 SV=3)

HSP 1 Score: 120.168 bits (300), Expect = 1.352e-26
Identity = 72/210 (34.29%), Postives = 108/210 (51.43%), Query Frame = 2
Query:   20 ISLATTLPFLILALASNSLLVLGTSSVPEPLLDVHGNKVESTIKYYIVSAI--WXXXXXXXXXXXXXXXYCPLDVIQLPSDMQNGIELTLSPYN-NSTIVRESTDLNLRFSVLLSGRDYCNEQPLWKVDNYDAASGKWFITTGGLDGHPGAETLLNWFKLEKIGNFPGIYKIVHCPSVCESCVKLCHNVGRSFEDGVRRLVLVRDDEPAF 640
            +SL+      +LA A+N LL     S P P+LD+ G K+ +   YYIV  +                   CP  V+Q   ++ +   L   P N    +VR STDLN+ FS  +  R       +W++D YD ++G++F+T GG+ G+PG ET+ +WFK+E+     G YK+V CP+VC SC   C +VG   +   RR + + D   AF
Sbjct:    7 LSLSFFFVSALLAAAANPLLS-AADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCR--WTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCG-SGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAF 212          
BLAST of CF835333 vs. ExPASy Swiss-Prot
Match: LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 7.185e-20
Identity = 64/179 (35.75%), Postives = 92/179 (51.40%), Query Frame = 2
Query:  101 PEPLLDVHGNKVESTIKYYIVSAIWXXXXXXXXXXXXXXXY-CPLDVIQLPSDMQNGIELTLSPYNN--STIVRESTDLNLRFSVLLSGRDYCNEQPLWKVDNYDAASGKWFITTGGLDGHPGAETLLNWFKLEKIGNFPGIYKIVHCPSVCESCVKLCHNVGRSFED-GVRRLVLVRD 625
            P+P++D+ G  V   + Y++VSAIW                 CPL V+Q P D  NG  +  S   N    IV ES DLN++F++ ++    CNE   WKVD +    G W +T GG  G+ G E+  + FK++K G  P  YK   CPS   + +  C+NV   F+   +RRL+L  D
Sbjct:    3 PKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEPIIFSAIKNVKDNIVFESVDLNVKFNITIN----CNETTAWKVDRFPGVIG-WTVTLGGEKGYHGFESTHSMFKIKKAG-LPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTND 173          
BLAST of CF835333 vs. ExPASy Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.714e-13
Identity = 57/185 (30.81%), Postives = 87/185 (47.03%), Query Frame = 2
Query:  104 EPLLDVHGNKVESTIKYYIVSAIWXXXXXXXXXXXXXXXYCPLDVIQLPSDMQNGIELTLS-PYNNSTIVRESTDLNLRFSVLLSGRDYCNEQPLWKVDNYDAASGKWFITTGGLDGHPGAETLLNWFKLEKIGNFPGIYKIVHCPSVCESCVKLCHNVGRSFEDGVRRLVLVRDDEPAFPVVLI 655
            E + D+ G  V    +YYIVSAI                 CP+ +IQ  SD+Q G+ +  S P  +   +   T+L + F      +  C E   W +      SG+  +  GG + HP  E +  +FK+EK+G+    YK+V CP   +S    C ++G ++E G R LVL   D+  F VV +
Sbjct:    4 EKVYDIEGYPVFLGSEYYIVSAIIGAGGGGVRPGRTRGSMCPMSIIQEQSDLQMGLPVRFSSPEESQGKIYTDTELEIEFVE----KPDCAESSKWVIVK---DSGEARVAIGGSEDHPQGELVRGFFKIEKLGSL--AYKLVFCP---KSSSGSCSDIGINYE-GRRSLVLKSSDDSPFRVVFV 175          
BLAST of CF835333 vs. ExPASy Swiss-Prot
Match: SPORB_IPOBA (Sporamin B OS=Ipomoea batatas GN=GSPO-B1 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.233e-12
Identity = 58/193 (30.05%), Postives = 89/193 (46.11%), Query Frame = 2
Query:   89 TSSVPEPLLDVHGNKVESTIKYYIVSAIW--XXXXXXXXXXXXXXXYCPLDVIQLPSDMQNGIELTLSPYN-NSTIVRESTDLNLRFSVLLSGRDYCNEQPLWKVDNYDAASGKWFITTGGLDGHPGAETLLNWFKLEKIGNFPGIYKIVHCPSVCESCVKLCHNVGRSFEDGVRRLVLVRDDEPAFPVVLIP 658
            T+S   P+LD++G++V +   YYIVSAIW                  C  DVI   SD  NG  +T++P +  +T+V  ST    RF++  +    C     W +  +D+ SG++F+  G            N FK+E + +    YKI    S C+   + C NVGR ++   R   L   + P F  V+ P
Sbjct:   34 TASSETPVLDINGDEVRAGENYYIVSAIWGAGGGGLRLVRLDSSSNECASDVIVSRSDFDNGDPITITPADPEATVVMPSTYQTFRFNI--ATNKLCVNNVNWGI-KHDSESGQYFVKAGEF-----VSDNSNQFKIEVVNDNLNAYKI----SYCQFGTEKCFNVGRYYDPLTRATRLALSNTP-FVFVIKP 213          
BLAST of CF835333 vs. ExPASy Swiss-Prot
Match: SPOR1_IPOBA (Sporamin A OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 5.514e-12
Identity = 56/193 (29.02%), Postives = 89/193 (46.11%), Query Frame = 2
Query:   92 SSVPEPLLDVHGNKVESTIKYYIVSAIW-XXXXXXXXXXXXXXXYCPLDVIQLPSDMQNGIELTLSPYN---NSTIVRESTDLNLRFSVLLSGRDYCNEQPLWKVDNYDAASGKWFITTGGLDGHPGAETLLNWFKLEKIGNFPGIYKIVHCPSVCESCVKLCHNVGRSFEDGVRRLVLVRDDEPAFPVVLIP 658
            +S   P+LD++G++V +   YY+VSAIW                 C  DVI  P+D+ NG  +T++P      ST+V  ST    RF++  +    C     W +  +D+ASG++F+  G            N FK+E +      YK+ +C    +     C+NVGR  +  +R   L   + P F  V+ P
Sbjct:   37 ASSETPVLDINGDEVRAGGNYYMVSAIWGAGGGGLRLAHLDMMSKCATDVIVSPNDLDNGDPITITPATADPESTVVMASTYQTFRFNI--ATNKLCVNNVNWGI-QHDSASGQYFLKAGEF-----VSDNSNQFKIELVDANLNSYKLTYCQFGSDK----CYNVGRFHDHMLRTTRLALSNSP-FVFVIKP 216          
BLAST of CF835333 vs. ExPASy Swiss-Prot
Match: SPOR2_IPOBA (Sporamin B OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.228e-11
Identity = 57/193 (29.53%), Postives = 89/193 (46.11%), Query Frame = 2
Query:   89 TSSVPEPLLDVHGNKVESTIKYYIVSAIW--XXXXXXXXXXXXXXXYCPLDVIQLPSDMQNGIELTLSPYN-NSTIVRESTDLNLRFSVLLSGRDYCNEQPLWKVDNYDAASGKWFITTGGLDGHPGAETLLNWFKLEKIGNFPGIYKIVHCPSVCESCVKLCHNVGRSFEDGVRRLVLVRDDEPAFPVVLIP 658
            T+S   P+LD++G++V +   YY+VSAIW                  C  DVI   SD  NG  +T++P +  +T+V  ST    RF++  +    C     W +  +D+ SG++F+  G            N FK+E + +    YKI    S C+   + C NVGR ++   R   L   + P F  V+ P
Sbjct:   34 TASSETPVLDINGDEVRAGENYYMVSAIWGAGGGGLRLVRLDSSSNECASDVIVSRSDFDNGDPITITPADPEATVVMPSTYQTFRFNI--ATNKLCVNNVNWGI-KHDSESGQYFVKAGEF-----VSDNSNQFKIEVVNDNLNAYKI----SYCQFGTEKCFNVGRYYDPLTRATRLALSNIP-FVFVIKP 213          
The following BLAST results are available for this feature:
BLAST of CF835333 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 7
Match NameE-valueIdentityDescription
ASP_THECC9.048e-3137.1921 kDa seed protein OS=Theobroma cacao GN=ASP PE=2... [more]
MIRA_RICDU1.352e-2634.29Miraculin OS=Richadella dulcifica PE=1 SV=3[more]
LSPI_CARPA7.185e-2035.75Latex serine proteinase inhibitor OS=Carica papaya... [more]
DRTI_DELRE1.714e-1330.81Kunitz-type serine protease inhibitor DrTI OS=Delo... [more]
SPORB_IPOBA3.233e-1230.05Sporamin B OS=Ipomoea batatas GN=GSPO-B1 PE=2 SV=1[more]
SPOR1_IPOBA5.514e-1229.02Sporamin A OS=Ipomoea batatas PE=2 SV=1[more]
SPOR2_IPOBA1.228e-1129.53Sporamin B OS=Ipomoea batatas PE=2 SV=1[more]
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Properties
Property NameValue
Genbank descriptionUCRCS03_01A24_r Washington Navel Orange Shoot Meristem cDNA Library Citrus sinensis cDNA clone CS_PEa01A24, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835333 ID=CF835333|Name=CF835333|organism=Citrus sinensis|type=EST|length=793bp
TGAAAACAACAAAATGAAGATTTCATTAGCAACAACACTCCCTTTCCTTA
TCCTTGCCTTGGCCTCAAACTCTTTGTTGGTCTTGGGCACCTCATCAGTT
CCTGAACCTCTCCTTGATGTCCACGGCAACAAGGTCGAATCCACCATTAA
GTACTACATAGTCTCAGCCATCTGGGGAGCTGGCGGTGGTGGAGTTAGCC
TACACGGTGGCAGAAACGGATATTGTCCGCTGGACGTTATCCAGTTACCT
TCTGATATGCAAAATGGAATCGAATTAACACTGTCACCGTATAACAATTC
TACAATTGTCCGAGAATCCACTGATCTCAACTTGAGATTCTCAGTGTTGC
TATCTGGACGTGACTATTGTAACGAACAACCGTTGTGGAAAGTTGATAAT
TATGACGCGGCATCAGGCAAATGGTTTATAACAACCGGGGGACTTGATGG
TCACCCTGGAGCTGAAACTTTGCTAAATTGGTTTAAGCTTGAGAAAATTG
GAAATTTTCCAGGTATATACAAAATCGTTCATTGTCCATCCGTTTGCGAG
TCTTGTGTCAAACTCTGCCACAACGTTGGCCGTTCGTTTGAGGATGGCGT
TCGACGTCTGGTTCTGGTTCGTGATGATGAGCCGGCATTTCCTGTTGTGT
TAATTCCCGCTACAGAAAGATCAACGTCTGTGTAATCAATGTACCCTAAC
CAGCTAGCGGTATTATTATTCTAAATAACGGACCAGGCTAGCTGCTCTTT
AATCTCTAGGATTAAAATTATGAATAAGTACCCTGTGATCCTT
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