CF835552

Overview
NameCF835552
Unique NameCF835552
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Washington Navel Orange Shoot Meristem cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F26_YEAST (Fructose-2,6-bisphosphatase OS=Saccharomyces cerevisiae GN=FBP26 PE=1 SV=2)

HSP 1 Score: 143.28 bits (360), Expect = 1.231e-33
Identity = 68/122 (55.74%), Postives = 92/122 (75.41%), Query Frame = -2 
Query:  313 FPKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEIQMGVTGVQEKRYK 678
            + K+QW+ALDE++AGVCDGMTYEEI+K  PE+++AR  DK  YRY  GESY DV+ RLEPVI+ELERQ   V++I+HQAVLR +YAYF + P +E P +  PLHT+I+++    G +  + K
Sbjct:  292 YKKLQWKALDELDAGVCDGMTYEEIEKEYPEDFKARDNDKYEYRYRGGESYRDVVIRLEPVIMELERQE-NVLIITHQAVLRCIYAYFMNVPQEESPWMSIPLHTLIKLEPRAYGTKVTKIK 412
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F261_BOVIN (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 OS=Bos taurus GN=PFKFB1 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 6.111e-33
Identity = 65/110 (59.09%), Postives = 89/110 (80.91%), Query Frame = -2 
Query:  352 GFPKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 681
            G P  QW+AL+EI+AGVC+ MTYEEI+++ PEE+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+   E+P+++ PLHT++++
Sbjct:  317 GLPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKL 425
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F26L_CHICK (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase OS=Gallus gallus PE=2 SV=2)

HSP 1 Score: 140.584 bits (353), Expect = 7.982e-33
Identity = 65/110 (59.09%), Postives = 88/110 (80.00%), Query Frame = -2 
Query:  352 GFPKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 681
            G P  QW+AL+EI+AGVC+ MTYEEI++  PEE+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+  +E+P++  PLHT++++
Sbjct:  316 GVPYEQWKALNEIDAGVCEEMTYEEIQERYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKSSEELPYLRCPLHTVLKL 424
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F261_RAT (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 OS=Rattus norvegicus GN=Pfkfb1 PE=1 SV=3)

HSP 1 Score: 140.584 bits (353), Expect = 7.982e-33
Identity = 65/110 (59.09%), Postives = 89/110 (80.91%), Query Frame = -2 
Query:  352 GFPKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 681
            G P  QW+AL+EI+AGVC+ MTYEEI+++ PEE+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+   E+P+++ PLHT++++
Sbjct:  317 GVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKL 425
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F261_MOUSE (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 OS=Mus musculus GN=Pfkfb1 PE=2 SV=2)

HSP 1 Score: 140.584 bits (353), Expect = 7.982e-33
Identity = 65/110 (59.09%), Postives = 89/110 (80.91%), Query Frame = -2 
Query:  352 GFPKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 681
            G P  QW+AL+EI+AGVC+ MTYEEI+++ PEE+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+   E+P+++ PLHT++++
Sbjct:  317 GVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKL 425
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F261_HUMAN (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 OS=Homo sapiens GN=PFKFB1 PE=1 SV=3)

HSP 1 Score: 140.584 bits (353), Expect = 7.982e-33
Identity = 65/110 (59.09%), Postives = 89/110 (80.91%), Query Frame = -2 
Query:  352 GFPKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 681
            G P  QW+AL+EI+AGVC+ MTYEEI+++ PEE+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+   E+P+++ PLHT++++
Sbjct:  317 GVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKL 425
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F26_RANCA (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase OS=Rana catesbeiana PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 1.042e-32
Identity = 64/108 (59.26%), Postives = 88/108 (81.48%), Query Frame = -2 
Query:  352 PKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 675
            P  QW+AL+EI+AGVC+ MTYEEI+ + PEE+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+  +E+P+++ PLHT++++
Sbjct:  318 PYEQWKALNEIDAGVCEEMTYEEIQDHFPEEFALRDQDKYRYRYPKGESYEDIVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKTAEELPYLKCPLHTVLKL 424
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F264_MACFA (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 OS=Macaca fascicularis GN=PFKFB4 PE=2 SV=3)

HSP 1 Score: 137.117 bits (344), Expect = 8.825e-32
Identity = 63/110 (57.27%), Postives = 87/110 (79.09%), Query Frame = -2 
Query:  352 GFPKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 681
            G P  QW+ L+EI+AGVC+ MTYEEI+ N P E+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+  +++P+++ PLHT++++
Sbjct:  315 GVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTVLKL 423
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F264_HUMAN (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 OS=Homo sapiens GN=PFKFB4 PE=2 SV=6)

HSP 1 Score: 137.117 bits (344), Expect = 8.825e-32
Identity = 63/110 (57.27%), Postives = 87/110 (79.09%), Query Frame = -2 
Query:  352 GFPKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 681
            G P  QW+ L+EI+AGVC+ MTYEEI+ N P E+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+  +++P+++ PLHT++++
Sbjct:  315 GVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTVLKL 423
[Close]
BLAST of CF835552 vs. ExPASy Swiss-Prot
Match: F263_RAT (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 OS=Rattus norvegicus GN=Pfkfb3 PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 8.825e-32
Identity = 64/108 (59.26%), Postives = 86/108 (79.63%), Query Frame = -2 
Query:  352 PKIQWRALDEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 675
            P  QW+AL+EI+AGVC+ +TYEEI+   PEEY  R++DK  YRYP GESY D++QRLEPVI+ELERQ   V+VI HQAVLR L AYF D+  +E+P+++ PLHT++++
Sbjct:  314 PYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQE-NVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKL 420
The following BLAST results are available for this feature:
BLAST of CF835552 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 27
Match NameE-valueIdentityDescription
F26_YEAST1.231e-3355.74Fructose-2,6-bisphosphatase OS=Saccharomyces cerev... [more]
F261_BOVIN6.111e-3359.096-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F26L_CHICK7.982e-3359.096-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F261_RAT7.982e-3359.096-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F261_MOUSE7.982e-3359.096-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F261_HUMAN7.982e-3359.096-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F26_RANCA1.042e-3259.266-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F264_MACFA8.825e-3257.276-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F264_HUMAN8.825e-3257.276-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F263_RAT8.825e-3259.266-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS03_01H11_f Washington Navel Orange Shoot Meristem cDNA Library Citrus sinensis cDNA clone CS_PEa01H11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835552 ID=CF835552; Name=CF835552; organism=Citrus sinensis; type=EST; length=706bp
TTTCTTTATTCATTTCTTCCCTTTTTTAATACATACACATATTGAATTTG
GGACCCTCTACATTCAATACTGTCCCAGACGTGCCCAACAGTAGCGGAAA
AAAAGGACCGCGAAATGTGATTAATTAGTAAGAGGAGCACTTGGCTTTTG
CCCCACAACTTCATAAAATTTTGGTTATTAGCATTAACATTGGTAACAAT
GAATGACACAGTTGAGTAATGAATTGCGATTCAATCCATCCAAATGTTGA
GTCGCATCGAGTTATAAAGCGACGAACTTCAGAATCCGTTGAAGCAATTT
TTAGTCCATGAGTTTGTATCTTTTCTCTTGGACACCTGTGACGCCCATCT
GTATTTCGATTATTGTATGGAGTGGCGTCTCAATGTGGGGAATTTCTTTG
AGAGGCCTATCAGCAAAATAAGCATATAAAGCTCTCAACACCGCCTGGTG
AGATATCACTACAACTGGTGCTCGTTGTCTTTCAAGTTCAATTATTACAG
GTTCTAGCCTTTGGATAACATCAAGATAAGATTCTCCACGTGGATACCGA
TACCTCAGTTTGTCCTTTTTACGAGCCTCATACTCCTCAGGCATATTTTT
CTTAATCTCTTCATACGTCATTCCATCACACACTCCAGCATTTATCTCAT
CAAGTGCACGCCATTGAATCTTTGGAAATCCGGCAATTGGACTCGCTGTC
AAAATT
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