CF835725

Overview
NameCF835725
Unique NameCF835725
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length708
Libraries
Library NameType
Washington Navel Orange Shoot Meristem cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835725_ssr226 microsatellite CF835725_ssr226:226..240. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig9675 contig Csv1_Contig9675:32..739. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CF835725 vs. ExPASy Swiss-Prot
Match: ELI_PEA (Early light-induced protein, chloroplastic OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 1.003e-51
Identity = 113/177 (63.84%), Postives = 132/177 (74.58%), Query Frame = 3
Query:  135 RSGANQLSSVK--YLPSLRRNVNLSVRCTAXXXXXXXXXXXX-PTIPKAE---PQPP--KKPRMSTGFSEVFAFSGPAPERINGRLAMIGFVAALGVEISKGQDVFAQLSDGGVAWFLGTSVLLTVASLVPLFKGVSVESKSDGFMTSDAELWNGRFAMLGLVALAFTEFVKGGTLV 641
            RS  NQ +++   Y+P+LRRNV+L VR  A             PT P A    PQP   + P+MST FS++ AFSGPAPERINGRLAMIGFVAA+GVEI+KGQ +  QLS GGVAWFLGTSVLL++ASL+P F+GVSVESKS   M+SDAE WNGR AMLGLVALAFTEFVKG +LV
Sbjct:   20 RSRVNQFTNIPSVYIPTLRRNVSLKVRSMAEGEPKEQSKVAVDPTTPTASTPTPQPAYTRPPKMSTKFSDLMAFSGPAPERINGRLAMIGFVAAMGVEIAKGQGLSEQLSGGGVAWFLGTSVLLSLASLIPFFQGVSVESKSKSIMSSDAEFWNGRIAMLGLVALAFTEFVKGTSLV 196          
BLAST of CF835725 vs. ExPASy Swiss-Prot
Match: DS22_CRAPL (Desiccation stress protein DSP-22, chloroplastic OS=Craterostigma plantagineum GN=DSP-22 PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.836e-37
Identity = 91/198 (45.96%), Postives = 126/198 (63.64%), Query Frame = 3
Query:   66 MAASLATQSMLASPVAARVTGIRRSGANQLSSVKY---LPSLRRNVNLSVRCTAXXXXXXXXXXXXP--TIPKAEPQPPKKPRMSTGFS-EVFAFSGPAPERINGRLAMIGFVAALGVEISKGQDVFAQLSDGGVAWFLGTSVLLTVASLVPLFKGVSVESKSDGFMTSDAELWNGRFAMLGLVALAFTEFVKGGTLV 641
            MA+S    ++ A     + T I R G N L   K    LP +RRN   +VR                  T        P +  ++T  + ++F+F G APERINGR AMIGFVAA+GVE++ G+DVF+Q+ +GGV WFL TS +L +A+L+P+++G+S E+K++GF  SDAE+WNGRFAM+GLVALAFTE+VKGG L+
Sbjct:    1 MASSTCYATIPAMSCRGQST-ITRFGPNNLFLGKQSYELPLMRRNAKFTVRSMREDNEKEEQQQQKQQQTHDGGPDLTPNRTEVTTKRTVDLFSFDGLAPERINGRSAMIGFVAAVGVELATGRDVFSQVFNGGVMWFLLTSAVLVLATLIPIYRGLSPEAKNNGFWNSDAEIWNGRFAMIGLVALAFTEYVKGGPLI 197          
BLAST of CF835725 vs. ExPASy Swiss-Prot
Match: ELI5_HORVU (High molecular mass early light-inducible protein HV58, chloroplastic OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 7.982e-33
Identity = 75/131 (57.25%), Postives = 93/131 (70.99%), Query Frame = 3
Query:  255 PTIPKAEPQPPKKPRMSTGFSEVFAFSGPAPERINGRLAMIGFVAALGVEISKGQDVFAQLS--DGGVAWFLGTSVLLTVASLVPLFKGVSVESKSDGFMTSDAELWNGRFAMLGLVALAFTEFVKGGTLV 641
            PT   A P+P  K + +    +  AFSGPAPERINGRLAM+GFVAAL VE ++G  +  Q+     G+AWFL T+ + +VASL+PL +G SVESKS G  ++DAELWNGRFAMLGLVALA TEF+ G   V
Sbjct:  100 PTTIPAAPKPVTK-KANPSVWDALAFSGPAPERINGRLAMVGFVAALSVEAARGGGLLDQVGMWSSGLAWFLATAGVFSVASLLPLLQGQSVESKSSGIWSADAELWNGRFAMLGLVALAATEFITGAPFV 229          
BLAST of CF835725 vs. ExPASy Swiss-Prot
Match: ELI9_HORVU (Low molecular mass early light-inducible protein HV90, chloroplastic OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.322e-32
Identity = 86/189 (45.50%), Postives = 111/189 (58.73%), Query Frame = 3
Query:   78 LATQSMLASPVAARVTGIRRSGANQLSSVKYLPSLRRNVNLSVRCTAXXXXXXXXXXXXPTIPKAEPQPPKKPRMSTGFSEVFAFSGPAPERINGRLAMIGFVAALGVEISKGQDVFAQLSDG-GVAWFLGTSVLLTVASLVPLFKGVSVESKSDGFMTSDAELWNGRFAMLGLVALAFTEFVKGGTLV 641
            +AT   ++S   A V  + RS A+   + + LP+L R   L VR               P+ P     PP KP+ ST   +  AFSGPAPERINGRLAM+GFV AL VE  +G  + +QL  G G AWF  T  +L++ASLVPL +G S E ++   M ++AELWNGRFAMLGLVALA TE + G   +
Sbjct:    1 MATMMSMSSFAGAAV--VPRSSASSFGA-RSLPALGRRA-LVVRAQTEG----------PSAP-----PPNKPKASTSIWDEMAFSGPAPERINGRLAMVGFVTALAVEAGRGDGLLSQLGSGTGQAWFAYTVAVLSMASLVPLLQGESAEGRAGAIMNANAELWNGRFAMLGLVALAATEIITGAPFI 170          
BLAST of CF835725 vs. ExPASy Swiss-Prot
Match: ELI6_HORVU (Low molecular mass early light-inducible protein HV60, chloroplastic OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.174e-32
Identity = 68/117 (58.12%), Postives = 84/117 (71.79%), Query Frame = 3
Query:  282 PPKKPRMSTGFSEVFAFSGPAPERINGRLAMIGFVAALGVEISKGQDVFAQLSDG-GVAWFLGTSVLLTVASLVPLFKGVSVESKSDGFMTSDAELWNGRFAMLGLVALAFTEFVKG 629
            PP KP+ ST   +  AFSGPAPERINGRLAM+GFV AL VE  +G  + +QL  G G AWF  T  +L++ASLVPL +G S E ++   M ++AELWNGRFAM+GLVALA TE + G
Sbjct:   45 PPNKPKASTSIWDAMAFSGPAPERINGRLAMVGFVTALAVEAGRGDGLLSQLGSGTGQAWFAYTVAMLSMASLVPLLQGESAEGRAGAIMNANAELWNGRFAMIGLVALAATEIITG 161          
BLAST of CF835725 vs. ExPASy Swiss-Prot
Match: CBR_DUNBA (Carotene biosynthesis-related protein CBR, chloroplastic OS=Dunaliella bardawil GN=CBR PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.909e-18
Identity = 61/128 (47.66%), Postives = 73/128 (57.03%), Query Frame = 3
Query:  258 TIPKAEPQPPKKPRMSTG--FSEVFAFSGPAPERINGRLAMIGFVAALGVEISKGQDVFAQLSDGGVAWFLGTSVLLTVASLVPLFKGVSVESKSD--GFMTSDAELWNGRFAMLGLVALAFTEFVKG 629
            T P + P PP  P        +EV  FSG APE INGRLAM+GFVAALG E+S G+ V  QL D      L T VL + ASL+P F       K D  G  T DAE+ NGRFAM+G  A+   E ++G
Sbjct:   47 TPPPSSPSPPPPPPTPAAPTVTEVMGFSG-APEIINGRLAMLGFVAALGAELSTGESVLTQLGDQPTLIAL-TFVLFSAASLIPAF----ARRKGDAMGPFTPDAEMTNGRFAMIGFAAMLVYEGIQG 168          
The following BLAST results are available for this feature:
BLAST of CF835725 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 6
Match NameE-valueIdentityDescription
ELI_PEA1.003e-5163.84Early light-induced protein, chloroplastic OS=Pisu... [more]
DS22_CRAPL1.836e-3745.96Desiccation stress protein DSP-22, chloroplastic O... [more]
ELI5_HORVU7.982e-3357.25High molecular mass early light-inducible protein ... [more]
ELI9_HORVU2.322e-3245.50Low molecular mass early light-inducible protein H... [more]
ELI6_HORVU5.174e-3258.12Low molecular mass early light-inducible protein H... [more]
CBR_DUNBA1.909e-1847.66Carotene biosynthesis-related protein CBR, chlorop... [more]
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Properties
Property NameValue
Genbank descriptionUCRCS03_01M10_r Washington Navel Orange Shoot Meristem cDNA Library Citrus sinensis cDNA clone CS_PEa01M10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835725 ID=CF835725|Name=CF835725|organism=Citrus sinensis|type=EST|length=708bp
CGCCGACCTGAAGCACCCAATTCCCGAATTATACTTTGTTCTGTTTTCTA
CTTCATAAATTAAAAATGGCCGCATCACTTGCTACTCAATCTATGCTGGC
AAGTCCTGTAGCAGCTCGTGTGACTGGTATCAGGAGATCCGGGGCAAACC
AACTTTCCTCCGTCAAGTACTTGCCTAGTCTGCGTAGAAATGTCAACTTA
TCCGTGCGCTGCACGGCAGAGGATGAGCAGCAGCAGCAGCAGAAAGATGA
TCAACCAACCATACCGAAAGCTGAGCCTCAGCCACCAAAGAAGCCGCGGA
TGAGCACGGGGTTCTCTGAGGTGTTTGCGTTCAGCGGGCCGGCACCGGAG
AGGATAAACGGGAGGCTTGCGATGATTGGGTTCGTGGCTGCATTGGGGGT
GGAGATATCCAAGGGTCAGGATGTGTTTGCCCAGTTATCAGACGGTGGGG
TTGCCTGGTTCTTGGGGACCAGTGTTTTGCTCACGGTTGCGTCCCTGGTT
CCTTTGTTTAAAGGTGTCAGCGTGGAGTCTAAATCGGATGGGTTCATGAC
TTCGGATGCTGAGTTGTGGAATGGGAGGTTTGCTATGCTGGGCCTTGTTG
CTTTGGCTTTCACCGAGTTTGTTAAGGGTGGCACCCTTGTTTAAGCTTAA
TTTTGTTTAGATCTGTAGATCTGTATTTTTTTTAACTAATTAATGTATGA
GATCCTAC
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