CK739901

Overview
NameCK739901
Unique NameCK739901
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length290
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2225 contig Csv1_Contig2225:119..412. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC10_ARATH (Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis thaliana GN=UBC10 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.752e-27
Identity = 42/50 (84.00%), Postives = 44/50 (88.00%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPF**PHMQEVY 230
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP   P+   V+
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVF 50

HSP 2 Score: 53.5286 bits (127), Expect = 3.752e-27
Identity = 22/27 (81.48%), Postives = 24/27 (88.89%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PYAGGVFLVTIHFPPDYPF+ PK  F
Sbjct:   43 SPYAGGVFLVTIHFPPDYPFKPPKVAF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC9_ARATH (SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana GN=UBC9 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.278e-27
Identity = 42/50 (84.00%), Postives = 44/50 (88.00%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPF**PHMQEVY 230
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP   P+   V+
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVF 50

HSP 2 Score: 52.373 bits (124), Expect = 8.278e-27
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PY+GGVFLVTIHFPPDYPF+ PK  F
Sbjct:   43 SPYSGGVFLVTIHFPPDYPFKPPKVAF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC8_ARATH (Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana GN=UBC8 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.278e-27
Identity = 42/50 (84.00%), Postives = 44/50 (88.00%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPF**PHMQEVY 230
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP   P+   V+
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVF 50

HSP 2 Score: 52.373 bits (124), Expect = 8.278e-27
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PY+GGVFLVTIHFPPDYPF+ PK  F
Sbjct:   43 SPYSGGVFLVTIHFPPDYPFKPPKVAF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC28_ARATH (Ubiquitin carrier protein E2 28 OS=Arabidopsis thaliana GN=UBC28 PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.278e-27
Identity = 42/50 (84.00%), Postives = 44/50 (88.00%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPF**PHMQEVY 230
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP   P+   V+
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVF 50

HSP 2 Score: 52.373 bits (124), Expect = 8.278e-27
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PY+GGVFLVTIHFPPDYPF+ PK  F
Sbjct:   43 SPYSGGVFLVTIHFPPDYPFKPPKVAF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC4_SOLLC (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.078e-26
Identity = 42/50 (84.00%), Postives = 44/50 (88.00%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPF**PHMQEVY 230
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP   P+   V+
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVF 50

HSP 2 Score: 51.9878 bits (123), Expect = 1.078e-26
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PYAGGVFLV+IHFPPDYPF+ PK  F
Sbjct:   43 SPYAGGVFLVSIHFPPDYPFKPPKVAF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC11_ARATH (Ubiquitin-conjugating enzyme E2 11 OS=Arabidopsis thaliana GN=UBC11 PE=2 SV=2)

HSP 1 Score: 84.3445 bits (207), Expect = 1.156e-25
Identity = 38/40 (95.00%), Postives = 40/40 (100.00%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            MASKRILKELKDLQKDPP++CSAGPVAEDMFHWQATIMGP
Sbjct:    1 MASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGP 40

HSP 2 Score: 51.6026 bits (122), Expect = 1.156e-25
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PYAGGVFLV+IHFPPDYPF+ PK  F
Sbjct:   43 SPYAGGVFLVSIHFPPDYPFKPPKVSF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC29_ARATH (Ubiquitin carrier protein E2 29 OS=Arabidopsis thaliana GN=UBC29 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 4.954e-23
Identity = 33/40 (82.50%), Postives = 37/40 (92.50%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            MA++RILKELK+LQ+DPP SCSAGP  EDMFHWQATIMGP
Sbjct:    1 MATRRILKELKELQRDPPVSCSAGPTGEDMFHWQATIMGP 40

HSP 2 Score: 50.0618 bits (118), Expect = 4.954e-23
Identity = 20/27 (74.07%), Postives = 23/27 (85.19%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PY+GGVFLV IHFPPDYPF+ PK  F
Sbjct:   43 SPYSGGVFLVNIHFPPDYPFKPPKVVF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC30_ARATH (Ubiquitin carrier protein E2 30 OS=Arabidopsis thaliana GN=UBC30 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.405e-22
Identity = 32/40 (80.00%), Postives = 35/40 (87.50%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            MASKRI KEL+DLQ+DPP SCSAGP  +DMF WQATIMGP
Sbjct:    1 MASKRINKELRDLQRDPPVSCSAGPTGDDMFQWQATIMGP 40

HSP 2 Score: 51.9878 bits (123), Expect = 2.405e-22
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +P+AGGVFLVTIHFPPDYPF+ PK  F
Sbjct:   43 SPFAGGVFLVTIHFPPDYPFKPPKVAF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UB2D4_HUMAN (Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens GN=UBE2D4 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.742e-20
Identity = 33/50 (66.00%), Postives = 38/50 (76.00%), Query Frame = 3 
Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPF**PHMQEVY 230
            MA KRI KEL DLQ+DPP  CSAGPV +D+FHWQATIMGP   P+   V+
Sbjct:    1 MALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVF 50

HSP 2 Score: 45.8246 bits (107), Expect = 2.742e-20
Identity = 18/27 (66.67%), Postives = 21/27 (77.78%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PY GGVF +TIHFP DYPF+ PK  F
Sbjct:   43 SPYQGGVFFLTIHFPTDYPFKPPKVAF 69
[Close]
BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC4_YEAST (Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae GN=UBC4 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 4.639e-20
Identity = 31/49 (63.27%), Postives = 40/49 (81.63%), Query Frame = 3 
Query:   84 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPF**PHMQEVY 230
            +SKRI KEL DL++DPPTSCSAGPV +D++HWQA+IMGP   P+   V+
Sbjct:    3 SSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVF 51

HSP 2 Score: 45.4394 bits (106), Expect = 4.639e-20
Identity = 18/27 (66.67%), Postives = 22/27 (81.48%), Query Frame = 2 
Query:  209 APYAGGVFLVTIHFPPDYPFQAPKGCF 289
            +PYAGGVF ++IHFP DYPF+ PK  F
Sbjct:   44 SPYAGGVFFLSIHFPTDYPFKPPKISF 70
The following BLAST results are available for this feature:
BLAST of CK739901 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 41
Match NameE-valueIdentityDescription
UBC10_ARATH3.752e-2784.00Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis ... [more]
UBC9_ARATH8.278e-2784.00SUMO-conjugating enzyme UBC9 OS=Arabidopsis thalia... [more]
UBC8_ARATH8.278e-2784.00Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis t... [more]
UBC28_ARATH8.278e-2784.00Ubiquitin carrier protein E2 28 OS=Arabidopsis tha... [more]
UBC4_SOLLC1.078e-2684.00Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum ... [more]
UBC11_ARATH1.156e-2595.00Ubiquitin-conjugating enzyme E2 11 OS=Arabidopsis ... [more]
UBC29_ARATH4.954e-2382.50Ubiquitin carrier protein E2 29 OS=Arabidopsis tha... [more]
UBC30_ARATH2.405e-2280.00Ubiquitin carrier protein E2 30 OS=Arabidopsis tha... [more]
UB2D4_HUMAN2.742e-2066.00Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens... [more]
UBC4_YEAST4.639e-2063.27Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6293 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE61F04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK739901 ID=CK739901; Name=CK739901; organism=Citrus sinensis; type=EST; length=290bp
CGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAAGGTTCGTTGTAG
TTTCAAATTTTAATAATCCCCTGAAGAGTCATGGCTTCAAAGCGTATTTT
GAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTCTTGCAGCGCTG
GCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAATTATGGGGCCC
TTCTGATAGCCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTT
CCTCCTGATTATCCCTTTCAAGCCCCCAAAGGTTGCTTTT
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